考虑碳化影响的纤维增强再生混凝土吸水特性研究

考虑再生粗骨料取代率与聚丙烯纤维体积率的影响,开展了再生混凝土快速碳化以及碳化后的毛细吸水试验研究。试验结果表明:混凝土碳化深度随着再生粗骨料取代率的增加而增大,随聚丙烯纤维体积率增大呈先下降后上升的趋势,纤维会降低再生混凝土的碳化深度。再生混凝土毛细吸水系数随碳化程度的增加先减小后增大;随再生粗骨料取代率增加而增大;随聚丙烯纤维体积率增加先降低后增大。碳化对再生混凝土初始毛细吸水系数的影响大于后期吸水系数,且混凝土孔隙率越大,碳化对初始吸水系数的影响越显著。最后,建立了考虑碳化影响的再生混凝土初始毛细吸水系数预测模型。

Molecular Structure of α-Phenylselanyl-α-Acetylmethylenen Triphenylarsenium

The molecular structure of the title compound, ph3AsC (COCH3 )Seph, has been determined by X--ray diffraction. Crystallographic data: Mr= 517. 36'monoclinic, P21,/c, a=11. 499(4), b=9. 619(4), c=20. 919(7),A, β=98. 82(2)°,V= 2286(l) A 3, Z=4, Dc= 1. 509 g/cm3, μ(MoKa) = 3. 073 cm-1, F(000) = 1040and the final R=0. 042, Rw= 0. 041. In the molecule, the geometry of arsenic atom isa distorted tetrahedron, the bonds of Se--C (3) and As--C(31 ) have striking characteristics of double bond, the delocalization of electronic density occurs to be restrictedfrom O(1 ) to Se atoms.

Study on the Synthesis of Phenylacetic Acid by Carbonylation of Benzyl Chloride under Normal Pressure

The influences of some factors on the yield of phenylacetic acid in thecarbonylation of benzyl chloride were studied in this paper. These factors included the variety andcontent of catalyst, and that of solvent, way of material feeding, reaction temperature, sodiumhydrate concentration, triphenylphosphine content, presence of surfactant, the ratio of organicphase volume to aqueous phase volume etc. The optimum reaction conditions were found to be: with aone-time pour of 0.15 g Pd(PPh_3)_2Cl_2, 0.24 g PPh_3, 75 ml NaOH of 3.5 mol/L, 20 ml benzylchloride and 55 ml n-butyl alcohol, and the reaction was carried out at 50℃ for about 3 hours. Theas-obtained yield of phenylacetic acid was as high as 97.6%. In addition, the influences of thepresence of phenylacetic acid and air in the reaction system were also studied. The results showedthat the presence of air in the system and the entrainment of phenylacetic acid in the circulatingorganic phase had great influence on the reaction rate, the stability and performance of catalystand the yield of phenylacetic acid.

Synthesis, Crystal Structure and Luminescence Property of a Cd(Ⅱ) Complex Assembled by 4-Carboxymethoxy Phenylacetic Acid

A new structure with the molecular formula [CdL]n was formed by CdSO4 with 4-carboxymethoxy phenylacetic acid （H2L） through the hydrothermal method. The complex was characterized by elemental analysis and infrared spectroscopy. The structure of the complex was determined by single-crystal X-ray diffraction, which is of monoclinic system, space group P21/c with a = 10.3887（2）, b = 7.10710（10）, c = 14.7212（2） , β = 120.6940（10）°, V = 934.65（3） 3, Dc = 2.278 g·cm-3, Z = 4, F（000） = 624, S = 1.022, the final R = 0.0187 and wR = 0.0487 for 2000 observer reflections （Ⅰ 〉 2σ（Ⅰ））. The center metal ion Cd（Ⅱ） in the complex is six-coordinated in a distorted octahedral geometry, and is connected with L ligands to form a 3D fishing net structure, which is a novel （3,6） network topology. The luminescence of the complex has been investigated, and the result reveals that it displays luminescent property in the voilet region.

Syntheses and Crystal Structures of Three Ag(Ⅰ) Complexes with Chloro-phenylacetic Acid and Nitrogen Heterocyclic Ligand

Three new coordination polymers {[Ag2（2,4-DCPA）（4,4＂-bipy）2（NO3）].（H20）}, （1）, [Ag（2,4-DCPA）（bpp）], （2） and {[Ag（4-DCPA）（bpp）]＇（H20）}, （3）, were synthesized by evaporation methods using 2,4-dichlorophenylacetic acid （2,4-DCPA）, 4-chlorophenylacetic acid （4-DCPA）, 4,4＂-dipyridyl （4,4＂-bipy）, 1,3-bis（4-pyridyl）propane（bpp） and AgNO3. Compound 1 crystallizes in monoclinic, space group P21/c with a = 10.035（2）, b = 17.796（3）, c = 16.448（2） A, fl = 104.086（2）°, V= 2705.9（2） A3, Dc = 1.893 g/cm-3, C28H23C12NsO6Ag2, Mr = 812.15, F（000） = 1608,μ（MoKot） = 1.615 mm-1, Z = 4, R = 0.0310, wR = 0.0832 for 4357 observed reflections （I 〉 20（/））, and R = 0.0362, wR = 0.0873 for all data. Compound 2 crystallizes in monoclinic, space group P21/c with a = 9.840（2）, b = 24.971（5）, c = 9.3301（2）°, fl = 117.504（2）°, V= 2033.5（7） A3, Dc = 1.666 g/cm3, C2tH19C12N202Ag, Mr = 510.15, F（000） = 1024,/I（MoKa） = 1.273 mml, Z= 4, R = 0.0315 and wR = 0.0680 for 2853 observed reflections （1 〉 20（/）） and R = 0.0454, wR = 0.0736 for all data. Compound 3 crystallizes in monoclinic, space group P21/n with a = 15.472（4）, b = 9.000（2）, c = 16.262（4） A, fl = 112.049（4）°, V = 2099.0（8） A3, Dc = 1.562 g/cm-3, C2H22C1N203Ag, Mr = 493.73, F（000） = 1000, μ（MoKa） = 1.111 mm-1, Z = 4, R = 0.0296, wR = 0.0821 for 3059 observed reflections （I 〉 2δ-（I））, and R = 0.0398, wR = 0.0986 for all data. The complexes were characterized by elemental analysis, FT-IR, thermogravimetrie analysis （TGA） and X-ray single-crystal structure analysis. Fluorescence properties of complex 3 and PXRD of 1 and 2 have been studied. As a result, in complex 1, the Ag（I） ion is surrounded by two nitrogen atoms from two bipy ligands to form an infinite chain, and adjacent Ag-bipy chains produce ＂＇rungs＂ of a ladder by silver ions contact. And in complexes 2 and 3, the two-dimensional ,β-sheet like layers are obtained by Ag……Ag interactions.

Structure and Properties of Polytetrafluorethylene and Polyurethane Layered Membrane for Protective Clothing

This paper presents a new idea for intensifying protective and stretch recovery properties of micro porous polytetrafluorethylene and hydrophilic polyurethane (PTFE/PU) layered membrane through a co-stretching process. The structure and properties of co-stretching PTFE/PU layered membrane and coated PTFE/PU layered membrane by means of directly coating the PU on the PTFE membrane were investigated using Electron Microscope, Universal Materials Testing Machine, and the water vapor permeability (WVP) was measured according to absorption method of water vapor of GB/T 12704-91. Contrasted to PU coating process, the PU membrane on the co-stretching PTFE/PU membrane is nonporous because of heat treatment process, which can prevent the SARS virus from permeating the Co-stretching PTFE/PU membrane. The stretch and recovery properties of the Co-stretching PTFE/PU membrane is at least 66% after being stretched to 50% of its original length in transverse directions and that of the coated PTFE/PU membrane is 52%. The WVP of the Co-stretching PTFE/PU membrane is 13 523 g/24 h·m^2. The results suggest that when Co-stretching PTFE/PU membrane is laminated to a stretchable fabric, the fabric would have excellent stretch and recovery properties while waterproof and being permeable to water vapor. So, the Co-stretching PTFE/PU membrane laminated fabric will be a comfortable protective clothing material.

Crystal Structure and Characterization of Neodymium Phenylacetate Hydrate

The crystal structure of Nd(ph-CH_2COO)_3H_2O has been determined by X-ray diffraction technique.The crystal behmgs to orthorhombic system with space group P_(na)2_1,cell parameters a=0.7983(1)nm, h=1.3957(1)nm,c=2.0282(2)nm;V=2.2598(15)nm^3,Z=4.The Mechanism and enthalpy changes of thermal decomposition reaction of the title complex have been studied by TG-DTG-DTA and DSC.

Effects of Phenylacetate on Cell Proliferation and Homeobox Genes Expression in the HCT-8 Colorectal Carcinoma Cell Line

To study the effects of phenylacetate （PA） on cell proliferation and homeobox （HOX） genes expression in the colorectal carcinoma HCT-8 cell line, HCT-8 cells were grown in the presence or absence of PA. The cellular proliferation inhibition was evaluated by the MTT assay. Twenty-two HOX genes were divided into three groups （ P1, P2, P3） according to their primer sequences, and the samples of cells were analyzed for the HOX genes＇ mRNA expression by means of the semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR）. The level of the HOX genes＇ expression was expressed as the ratio expression rate of HOX gene to the β-actin. HCT-8 cells were treated with 1.0-5.0 mmol/L PA for 24-72 h. With the increase of the PA concentration or the prolongation of the treating time, the cell proliferation is inhibited in a dose- and time-dependent manner. The P1 group mRNA＊ expression（0. 5781 ±0. 0836） is significantly lower than that of the untreated group （0. 7701 ± 0. 0883 ） in HCT-8 cells （p 〈 0. 001 ）. Both the mRNA expressions of groups P2 （0. 3941 ± 0. 0819） and P3 （0. 5601 ± 0. 0736） in the PA treated group are significantly higher than those of the untreated groups P2（0. 1221±0. 0782） and P3 （0. 1806 ± 0. 0811 ） in HCT-8 cells（p 〈 0. 001）. PA could effectively inhibit cell proliferation by regulating the HOX genes expression and the mechanisms of the PA action are correlated with the transcription process in HCT-8 cells.

Inhibitory Effects of Phenylacetic Acid on Proliferation of Human Pancreatic Carcinoma BXPC-3 Cells by the Regulation of Adenosine Deaminases Acting on RNA

The effect and mechanism of phenylacetic acid on the proliferation of pancreatic carcinoma cells were investigated in cultured pancreatic carcinoma BXPC-3 cells by means of 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay and flow cytometry assay.The results show that the treatment of pancreatic carcinoma cells with phenylacetic acid significantly inhibited the cell proliferation in time-dependent and dose-dependent manners.The proliferation of BXPC-3 cells was inhibited at the stage of S phase,the cells at the end stage of S phase were accumulated abundantly,and thus DNA synthesis could not be accomplished entirely.In addition,the expression of adenosine deaminases acting on RNA（ADARs） mRNA in BXPC-3 cells and pancreatic carcinoma specimen were detected by RT-PCR.Having been treated with phenylacetic acid,ADAR2 mRNA in BXPC-3 cells was significantly decreased,the differences were of statistical significance（P0.01）.Taken together,these results suggest that phenylacetic acid may likely regulate the proliferation of pancreatic carcinoma cells through the regulation of ADAR2 mRNA expression.

THE EFFECT OF PHENYLACETATE ON THE EXPANSION AND CYTOTOXIC ACTIVITY OF ADHERENT LAK CELLS FROM PATIENTS WITH HEPATOCELLULAR CARCINOMA

Objective: To improve the preparation of adherent lymphokine-activated killer (A-LAK) cells and study the synergistic anti-tumor effect of phenylacetate (PA) and A-LAK cells. Methods: A-LAK cells were obtained from peripheral blood mononuclear cells (PBMC) of patients with hepatocellular carcinoma (HCC) by using L-phenylalanine methyl ester (PME) to deplete immunosuppressive monocytes. The proliferation of SMMC7721 cell line treated with PA was studied. A-LAK cells were treated with the supernatant of SMMC7721 cells which had been pretreated with PA and the changes of the proliferation and anti-tumor activity of A-LAK cells were investigated. Results: The expansion of A-LAK cells was significantly higher than that of non-adherent LAK (NA-LAK) cells as well as regular LAK cells. The growth of SMMC7721 cells was significantly suppressed by PA. The supernatant of cultured tumor cells intensively suppressed the proliferation and cytotoxicity of A-LAK cells, but the suppressive effect of supernatant treated with PA previously was decreased. Conclusion: A-LAK cells could be simply prepared by using PME, and showed a synergistic anti-tumor effect with the combination of PA.

Increasein the Activity of Human Breast CancerCells to Induce Cytotoxic T Lymphocytes by Sodium Phenylacetate

The effects of differentiation inducer sodium phenylacetate on antigen expression of human breas cancer MDA-453 cells and their activity to induce allospecific cytotoxic T lymphocytes (CTL) in peripheral blood mononuclear cells (PBMC) were investigated. The results showed that sodium phenylacetate could the expression of HLA-A2 molecules, intercellular adhesion molecule-1 (ICAW-1) and protein HER-2/neu on the surface of MDA-453 cells. In mixed allogenic PBMC/MDA-453 cell (shared the same HLA-A2 locus) culture in vitro, MDA-453 cells could stimulate PBMC growth in the presence of low dose of interleukin-2 (IL-2 ), but sodium phenylacetate-treated MDA-453 cells were more potent for stimulating PBMC growth than the untreated cells. PBMC obtained from frow culture at 7th day could kill MDA-453 cells. The cytotoxicity of PBMC obtained form the culture at 21st day was restricted to MDA-453 cells (PBMC could hardly kill K562 and Raji cells), so the PBMC at this time could be called allospecific CTL. Sodium phenylacetate-treated MDA-453 cells were more potent for inducing allospecific CTL than the untreated cells in mixed allogenic PBMC/MDA-453 cell culture. In summary, sodium phenylacetate could increase the activity of breast cancer MDA-453 cells to induce allospecific CTL with a corresponding effect on antigen expression.

Phenylacetylglutaminate and Phenylacetate in Combination Upregulate VDUP1, Cause Cell Cycle Blockade and Apoptosis in U87 Glioblastoma Cells

Phenylacetylglutaminate (PG) and Phenylacetate (PN) are metabolites of Phenylbutyrate (PB) and are constituents of antineoplaston AS2-1. These are sodium salts of amino acid derivative and carboxylic acid that inhibit the growth of neoplastic cells without growth inhibitory effect in normal cells. The aim of this study was to identify molecular pathways involved in the anti-proliferative effect of antineoplastons. Using a total human genome microarray we have found that 1) Vitamin D3 upregulated protein (VDUP1) is significantly upregulated in response to PG and PN in the U87 glioblastoma cells;2) Isobologram analysis shows that PG and PN act in an additive or synergistic manner to effectively suppress proliferation of U87 cells;3) PG and PN cause cell cycle arrest, changes in expression of several cell cycle genes and suppress expression and activity of the G2/M checkpoint kinase, CHK1. The multiple cellular targets possibly make these compounds effective anti-proliferative agents. We propose that PG and PN in combination target important cellular pathways and upregulate VDUP1 leading to detachment-induced apoptosis in cancer cells.

ADSORPTION OF PHENYLACETIC ACID ON MACROPOROUS POLYMERIC ADSORBENTS

Several macroporous polymeric adsorbents (NDA-999, XAD-8, X-5 and XAD-2) were employed in the study to adsorb phenylacetic acid from aqueous solution. Effect of salt and ambient temperature on adsorption was studied using NDA-999 adsorbent and the adsorption process conforms to Freundlich抯 model reasonably. Adsorption dynamics were conducted in batch experiments in order to make clear the mechanism of adsorption process. It is proved that the squared driving force mass transfer model can be adopted to elucidate the process. The treatment process of industrial wastewater containing high strength of phenylacetic acid was proposed for cleaner production of phenylacetic acid.

Bacteroides fragilis Supernatant Extracts Enriched in Phenylacetic Acid Induce a Cytotoxic Effect in Mammalian Cells

Bacteroides species are nearly half of the fecal flora community and some are host symbionts crucial to host nutrition and systemic immunity. Among Bacteroides species B. fragilis strains are considered to be the opportunistic ones, being the most isolated anaerobic bacteria in clinical samples. Cell-free supernatants of 65 B. fragilis strains were assayed and they were capable of inducing vacuolating phenotype on Vero cells lineage. The supernatant of the Bacteroides fragilis ATCC 23745 strain was elicited to have the strongest vacuolating effect on Vero cells monolayers and peritoneal macrophages. Some drastic cell alterations were observed, such as a general disorganization of cytoplasm and chromatin condensation, evidencing cell death. By transmission electron microscopy it was confirmed that the vacuoles observed were, in fact, swollen mitochondria. An immunocytochemical assay, TUNEL, was used to confirm this hypothesis and showed that Vero cells and peritoneal macrophages were dying by apoptotic process after exposition of B. fragilis cell-free supernatant. Physical analysis of the apoptotic factor has revealed properties similar to short-chain fatty acids. After gas chromatography and mass spectrometry analysis, phenylacetic acid (PA) was characterized as the major compound present in the most purified active fraction. We believe that the PA is responsible for the pro-apoptotic effect elicited by the supernatant of B. fragilis cultures.

高苯丙氨酸血症患儿尿中代谢物水平分析

目的通过检测高苯丙氨酸血症（HPA）患儿血苯丙氨酸（Phe）及尿苯丙酮酸、苯乙酸及苯乳酸水平，探讨其血Phe水平与尿代谢产物的关系。方法研究对象为55例HPA患儿，其中20例为轻度HPA（血Phe浓度〈360mmol／L）、35例为苯丙酮尿症（PKU，血Phe浓度360M200mmol／L）；血r,he水平检测采用串联质谱干血滤纸片法；尿苯丙酮酸、苯乙酸及苯乳酸水平检测采用尿素酶预处理气相色谱-质谱法。结果HPA患儿血Phe水平与尿苯丙酮酸、苯乙酸及苯乳酸水平呈正相关（r=0．61～0．84，P均〈0．01）。轻度HPA与PKL噫儿尿苯丙酮酸、苯乙酸及苯乳酸水平差异有统计学意义（P均〈0．01）；70％轻度HPA患儿及5．71％PKU患儿的尿苯丙酮酸、苯乙酸及苯乳酸水平正常。结论HPA患儿尿中苯丙酮酸、苯乙酸及苯乳酸水平的变化与血Phe浓度密切相关，单纯检测尿苯丙酮酸、苯乙酸及苯乳酸可能导致假阴性。

两种羟基苯乙酸对大豆萌发的化感效应研究

通过田间和室内试验相结合 ,利用高效液相色谱检测大豆根际土壤和残茬腐解液中的对羟基苯乙酸和间羟基苯乙酸的含量 ;在培养皿内研究两种酚酸对大豆生长抑制效应 ;研究了二者对大豆DNA熔点 (Tm)的影响 ,并采用SDS PAGE方法研究二者对根系蛋白合成的影响 .结果表明 ,在大豆根际土壤和残茬腐解液中检测到对羟基苯乙酸 ;两种羟基苯乙酸处理后 ,大豆侧根数和主根长均显著减少 ,表现出明显的化感抑制效应 ,间羟基苯乙酸的抑制效应更明显 ;两种羟基苯乙酸使大豆DNA的熔点 (Tm)下降 ,一些小分子蛋白的合成受到影响 ,从而抑制大豆生长 ,表现出典型的化感效应 .

纳米氧化镁颗粒对聚丙烯的性能调控

为开发新型的可回收直流电缆绝缘材料,在对聚丙烯(polypropylene,PP)作为高压直流电缆绝缘材料可行性研究的基础上,对纳米Mg O/PP复合材料的微观形貌与结构、热性能、空间电荷和直流击穿特性进行了详细研究。研究了纳米MgO含量对PP微观形貌与结构、热性能、电气性能等特性的影响,并通过测试电荷陷阱能级和密度分布解释了纳米MgO调控PP电气性能的机理。研究结果表明:纳米MgO添加不会明显改变PP的结晶度、晶型、熔融温度等参数,纳米Mg O/PP复合材料依然保持了PP优良的热性能,且复合材料的热分解起始温度相对于PP略有提高。纳米MgO颗粒的加入可以明显抑制PP中同极性电荷注入,减少空间电荷积聚。添加3 phr纳米MgO颗粒的复合材料具有最高的直流击穿强度,相对于纯PP增加了29.3%。热刺激电流测试结果表明纳米MgO添加可增加深陷阱密度,在电极附近形成屏蔽层并降低载流子迁移率,从而抑制同极性电荷注入并提高直流击穿场强。通过研究纳米MgO颗粒调控PP的微观结构、热性能和电气性能的规律,可以为新型可回收直流电缆绝缘材料的开发提供参考。

无机固体酸催化合成苯乙酸异丁酯

用一水硫酸氢钠催化苯乙酸和异丁醇的酯化反应 ,合成了苯乙酸异丁酯。研究结果表明 ,硫酸氢钠具有较高的催化活性。考察了苯乙酸 /异丁醇摩尔比、催化剂用量及反应时间对酯产率的影响。在典型反应条件(苯乙酸 /异丁醇 /硫酸氢钠的摩尔比 =1∶4∶0 .0 36 ,回流分水 2h)下 ,所得苯乙酸异丁酯的产率达 92 .7%。

碳酸二甲酯和醋酸苯酯合成碳酸二苯酯热力学分析

采用Benson基团贡献法,对碳酸二甲酯和醋酸苯酯合成碳酸二苯酯反应体系进行了反应焓变、反应熵变、吉布斯自由能变及平衡常数的计算。结果表明该反应体系较为复杂,为了提高碳酸二苯酯收率,需要深入研究反应工艺过程和开发高效新型催化剂。

苯乙酸钠增强肿瘤细胞表面HLA分子的表达

以分化绣导剂苯乙酸钠处理肿瘤细胞,以ELISA检测肿瘤细胞表面HLA Ⅰ、Ⅱ类分子表达的变化。结果发现MCF-7、MDA-453、MKN-45以及Hela细胞表面HLA Ⅰ类分子表达较低,而MKN-28和3AO细胞表面HLA Ⅰ类分子高表达。MDA-453、MKN-28、MKN-45、Hela以及3AO细胞表面亦表达HLA Ⅱ类分子,而MCF-7细胞表面缺乏HLA Ⅱ类分子。苯乙酸钠能够诱导MCF-7细胞表面表达HLA Ⅱ类分子;增强MCF-7细胞表面HLA Ⅰ类分子,MDA-453、MKN-28、MKN-45、Hela以及3AO细胞表面HLA Ⅰ、Ⅱ类分子的表达。并且肿瘤细胞表面HLA Ⅰ类分子的表达与苯乙酸钠的作用时间和剂量呈正相关。