AIM： To investigate the prognostic significance of phosphatase regenerating liver 3 （PRL-3） protein expression in gastric cancer.METHODS： PRL-3 expression in paraffin-embedded tumor specimens from 293 patients wit...AIM： To investigate the prognostic significance of phosphatase regenerating liver 3 （PRL-3） protein expression in gastric cancer.METHODS： PRL-3 expression in paraffin-embedded tumor specimens from 293 patients with gastric cancer was studied retrospectively by immunohistochemistry. Nonoclonal antibody specifically against PRL-3, 3B6, was obtained with hybridoma technique.RESULTS： Positive PRL-3 expression was detected in 43.3% （227 of 293） of gastric cancer cases. High expression of PRL-3 was positively correlated with tumor size, depth of invasion, vascular/lymphatic invasion, lymph node metastasis, high TNM stage and tumor recurrence. Patients with positive PRL-3 expression had a significantly lower 5-year survival rate than those with negative expression （28.3% vs 52.9%, P 〈 0.0001）. Patients who received curative surgery, and with positive PRL-3 expression had a significant shorter overall survival and disease-free disadvantage over patients with negative expression （hazard ratio of 16.7 and 16.6, respectively; P 〈 0.0001 for both）. Multivariate analysis revealed that PRL-3 expression was an independent prognostic indicator for overall and disease-free survival of gastric cancer patients, particularly for survival in TNM stage Ⅲ patients. CONCLUSION： PRL-3 expression is a new independent prognostic indicator to predict the potential of recurrence and survival in patients with gastric cancer at the time of tumor resection,展开更多
目的:研究miR495、miR551a干扰质粒对SGC7901胃癌细胞中促肝细胞再生磷酸酶-3(phosphatase of regenerating liver-3,PRL-3)表达的影响,探讨基因干扰在胃癌治疗中的价值.方法:分别体外培养稳定转染后的SGC7901细胞系及未经处理的SGC790...目的:研究miR495、miR551a干扰质粒对SGC7901胃癌细胞中促肝细胞再生磷酸酶-3(phosphatase of regenerating liver-3,PRL-3)表达的影响,探讨基因干扰在胃癌治疗中的价值.方法:分别体外培养稳定转染后的SGC7901细胞系及未经处理的SGC7901细胞系,取对数生长期瘤细胞,0.5mL(1×107/mL)细胞悬液接种于Balb/ca(nu/nu)裸鼠腹腔内,在SPF条件下饲养1mo后处死,观察裸鼠成瘤率、瘤体生长情况及腹膜转移等情况,并行组织病理学检查.取各组部分移植瘤行荧光定量PCR检测,对比各组miR495、miR551a及PRL-3mRNA的相对表达水平.结果:各组裸鼠成瘤率均100%,其中两实验组裸鼠一般情况及生存期均好于对照组.取各组移植瘤行荧光定量PCR检测显示,两实验组裸鼠miRNA表达量显著高于对照组,PRL-3mRNA表达量低于对照组.转染质粒组胃癌迁移能力明显减弱.结论:转染靶向干扰PRL-3表达的miR495、mi551a真核质粒可以明显抑制胃癌细胞体内转移侵袭能力.展开更多
目的观察慢病毒介导的sh RNA沉默肝再生磷酸酶-3(PRL-3)基因对结肠癌SW480细胞增殖、侵袭、凋亡的影响。方法实验分组为空白对照组、阴性对照组、转染组。将携带PRL-3 sh RNA的慢病毒载体转染结肠癌SW480细胞,建立稳定沉默PRL-3的细胞株...目的观察慢病毒介导的sh RNA沉默肝再生磷酸酶-3(PRL-3)基因对结肠癌SW480细胞增殖、侵袭、凋亡的影响。方法实验分组为空白对照组、阴性对照组、转染组。将携带PRL-3 sh RNA的慢病毒载体转染结肠癌SW480细胞,建立稳定沉默PRL-3的细胞株,real-time PCR检测转染后PRL-3 m RNA的相对表达水平。采用MTT法、平板克隆形成实验检测转染后细胞增殖能力;采用Transwell侵袭实验、侵袭小室法检测转染后细胞迁移及侵袭能力;采用流式细胞术检测转染后细胞凋亡率变化。结果稳定沉默PRL-3的细胞株构建成功,转染组PRL-3m RNA的相对表达水平低于空白对照组、阴性对照组(P<0.05),空白对照组、阴性对照组比较差异无统计学意义。PRL-3 sh RNA转染SW480细胞72 h后,转染组与空白对照组、阴性对照组比较,细胞增殖能力受到抑制,转染120 h时最明显(P<0.05)。转染组克隆形成能力较空白对照组、阴性对照组下降(P<0.05)。转染组与空白对照组、阴性对照组比较,细胞迁移、侵袭能力下降,凋亡率增加(P<0.05)。结论结肠癌SW480细胞转染PRL-3 sh RNA可减少PRL-3的表达,有效抑制SW480细胞增殖,促进其凋亡,PRL-3可能成为治疗结肠癌的靶基因。展开更多
基金Supported by Grant for Key Technology Research and Development Program 2002BA711A06National Basic Research Priorities Program 973 Project 1998051203the Ministry of Science and Technology of China,and grant H020920030390,from the Beijing Science and Technology Commission
文摘AIM： To investigate the prognostic significance of phosphatase regenerating liver 3 （PRL-3） protein expression in gastric cancer.METHODS： PRL-3 expression in paraffin-embedded tumor specimens from 293 patients with gastric cancer was studied retrospectively by immunohistochemistry. Nonoclonal antibody specifically against PRL-3, 3B6, was obtained with hybridoma technique.RESULTS： Positive PRL-3 expression was detected in 43.3% （227 of 293） of gastric cancer cases. High expression of PRL-3 was positively correlated with tumor size, depth of invasion, vascular/lymphatic invasion, lymph node metastasis, high TNM stage and tumor recurrence. Patients with positive PRL-3 expression had a significantly lower 5-year survival rate than those with negative expression （28.3% vs 52.9%, P 〈 0.0001）. Patients who received curative surgery, and with positive PRL-3 expression had a significant shorter overall survival and disease-free disadvantage over patients with negative expression （hazard ratio of 16.7 and 16.6, respectively; P 〈 0.0001 for both）. Multivariate analysis revealed that PRL-3 expression was an independent prognostic indicator for overall and disease-free survival of gastric cancer patients, particularly for survival in TNM stage Ⅲ patients. CONCLUSION： PRL-3 expression is a new independent prognostic indicator to predict the potential of recurrence and survival in patients with gastric cancer at the time of tumor resection,
文摘目的:研究miR495、miR551a干扰质粒对SGC7901胃癌细胞中促肝细胞再生磷酸酶-3(phosphatase of regenerating liver-3,PRL-3)表达的影响,探讨基因干扰在胃癌治疗中的价值.方法:分别体外培养稳定转染后的SGC7901细胞系及未经处理的SGC7901细胞系,取对数生长期瘤细胞,0.5mL(1×107/mL)细胞悬液接种于Balb/ca(nu/nu)裸鼠腹腔内,在SPF条件下饲养1mo后处死,观察裸鼠成瘤率、瘤体生长情况及腹膜转移等情况,并行组织病理学检查.取各组部分移植瘤行荧光定量PCR检测,对比各组miR495、miR551a及PRL-3mRNA的相对表达水平.结果:各组裸鼠成瘤率均100%,其中两实验组裸鼠一般情况及生存期均好于对照组.取各组移植瘤行荧光定量PCR检测显示,两实验组裸鼠miRNA表达量显著高于对照组,PRL-3mRNA表达量低于对照组.转染质粒组胃癌迁移能力明显减弱.结论:转染靶向干扰PRL-3表达的miR495、mi551a真核质粒可以明显抑制胃癌细胞体内转移侵袭能力.
文摘目的观察慢病毒介导的sh RNA沉默肝再生磷酸酶-3(PRL-3)基因对结肠癌SW480细胞增殖、侵袭、凋亡的影响。方法实验分组为空白对照组、阴性对照组、转染组。将携带PRL-3 sh RNA的慢病毒载体转染结肠癌SW480细胞,建立稳定沉默PRL-3的细胞株,real-time PCR检测转染后PRL-3 m RNA的相对表达水平。采用MTT法、平板克隆形成实验检测转染后细胞增殖能力;采用Transwell侵袭实验、侵袭小室法检测转染后细胞迁移及侵袭能力;采用流式细胞术检测转染后细胞凋亡率变化。结果稳定沉默PRL-3的细胞株构建成功,转染组PRL-3m RNA的相对表达水平低于空白对照组、阴性对照组(P<0.05),空白对照组、阴性对照组比较差异无统计学意义。PRL-3 sh RNA转染SW480细胞72 h后,转染组与空白对照组、阴性对照组比较,细胞增殖能力受到抑制,转染120 h时最明显(P<0.05)。转染组克隆形成能力较空白对照组、阴性对照组下降(P<0.05)。转染组与空白对照组、阴性对照组比较,细胞迁移、侵袭能力下降,凋亡率增加(P<0.05)。结论结肠癌SW480细胞转染PRL-3 sh RNA可减少PRL-3的表达,有效抑制SW480细胞增殖,促进其凋亡,PRL-3可能成为治疗结肠癌的靶基因。
