Background: Amazonian riverside communities consume large quantities of freshwater fish, comparable to marine fish consumption of Inuit, Scandinavian and Japanese populations. Few studies have considered the relation ...Background: Amazonian riverside communities consume large quantities of freshwater fish, comparable to marine fish consumption of Inuit, Scandinavian and Japanese populations. Few studies have considered the relation of high freshwater fish consumption and intake of omega-3 fatty acids (FA). Objective: The objective of the present study was to determine the profile of the concentrations of plasma phospholipid FAs and its relation with freshwater fish intake in 12 riverside communities in the Tapajós River basin (State of Pará, Brazilian Amazon). Design: This cross-sectional study included 333 adults (15 - 86 years old). Fish meal frequency was determined using a 7-day interview-administered dietary recall questionnaire. Fish were categorized as piscivorous and non-piscivorous fish on trophic level. Plasma phospholipid FAs were measured by capillary gas-liquid chromatography. Results: Participants consumed 5 to 6 fish meals a week, with twice as many non-piscivorous fish than piscivorous fish (4.4 fish/week vs 2.2 fish/week, respectively). The omega-3 FA levels in plasma phospholipids were low (EPA + DHA = 31.21 mg/L;%EPA + DHA = 2.59%). Omega-3 FAs increased with frequency of fish intake, and particularly with piscivorous fish, controlling for sociodemographic, anthropometric and lifestyle characteristics. DHA levels were greater in women than in men. Conclusions: Omega-3 FA in this Amazonian population increased with fish consumption, but although they consumed freshwater fish almost daily, the concentrations of omega-3 FA were relatively low and comparable to fish-eater communities for which fish is not a dietary mainstay. It is possible that nutrients present in marine, but not in certain freshwater fish species, may facilitate absorption of omega-3 FA. Sex and/or gender differences must be taken into account when assessing the relationship between fish consumption and plasma phospholipid omega-3 FA levels.展开更多
采用偏最小二乘法(Partial least squares,PLS)作为建模方法,对磷虾油近红外光谱的一阶微分(First-order difference,FD)、FD+SG(Savitzky-Golay,SG)滤波、FD+N(Norris,N)滤波、二阶微分(Second-order difference,SD)、SD+SG和SD+N等6...采用偏最小二乘法(Partial least squares,PLS)作为建模方法,对磷虾油近红外光谱的一阶微分(First-order difference,FD)、FD+SG(Savitzky-Golay,SG)滤波、FD+N(Norris,N)滤波、二阶微分(Second-order difference,SD)、SD+SG和SD+N等6种单一或复合方法进行处理,通过对不同方式处理后预测模型的交互验证均方根误差(Root mean square error of cross validation,RMSECV)、外部验证残差均方根(Root mean square error of external prediction,RMSEP)和外部验证用样品真实值的标准差(SD)与RMSEP的比值(The ratio of the RMSEP to standard deviation of reference data in the prediction,RPDEV)、建模相关系数(Correlation coefficient in calibration,RC)、交互验证相关系数(Correlation coefficient in cross validation,RCV)和外部验证相关系数(Correlation coefficient in external validation,REV)等参数比较,确定了磷虾油磷脂、EPA和DHA的近红外预测模型最佳处理方式为FD、FD和SD+N,酸价指标模型不需处理。在最优条件下,四种成分近红外预测模型的RC、REV和RCV,除了酸价的RCV略小(0.917)其余均达到0.95以上,同时,四种成分的RPDEV和RPDCV值,除酸价的RPDCV为2.365,略小于2.5,其余均符合大于2.5的要求,说明磷虾油磷脂、EPA和DHA的近红外预测模型预测准确度良好;RMSEC和RMSECV相差不大,说明模型稳定性较好。由于含量低、组成复杂等原因,磷虾油虾青素近红外检测模型的RC、RCV和REV均在0.60以下,说明近红外检测不适用于磷虾油中虾青素成分的快速检测。本文证实了近红外光谱技术可作为磷虾油中磷脂、EPA、DHA和酸价等主要指标的快速检测方法,是传统化学检测方法的有效替代和补充。展开更多
文摘Background: Amazonian riverside communities consume large quantities of freshwater fish, comparable to marine fish consumption of Inuit, Scandinavian and Japanese populations. Few studies have considered the relation of high freshwater fish consumption and intake of omega-3 fatty acids (FA). Objective: The objective of the present study was to determine the profile of the concentrations of plasma phospholipid FAs and its relation with freshwater fish intake in 12 riverside communities in the Tapajós River basin (State of Pará, Brazilian Amazon). Design: This cross-sectional study included 333 adults (15 - 86 years old). Fish meal frequency was determined using a 7-day interview-administered dietary recall questionnaire. Fish were categorized as piscivorous and non-piscivorous fish on trophic level. Plasma phospholipid FAs were measured by capillary gas-liquid chromatography. Results: Participants consumed 5 to 6 fish meals a week, with twice as many non-piscivorous fish than piscivorous fish (4.4 fish/week vs 2.2 fish/week, respectively). The omega-3 FA levels in plasma phospholipids were low (EPA + DHA = 31.21 mg/L;%EPA + DHA = 2.59%). Omega-3 FAs increased with frequency of fish intake, and particularly with piscivorous fish, controlling for sociodemographic, anthropometric and lifestyle characteristics. DHA levels were greater in women than in men. Conclusions: Omega-3 FA in this Amazonian population increased with fish consumption, but although they consumed freshwater fish almost daily, the concentrations of omega-3 FA were relatively low and comparable to fish-eater communities for which fish is not a dietary mainstay. It is possible that nutrients present in marine, but not in certain freshwater fish species, may facilitate absorption of omega-3 FA. Sex and/or gender differences must be taken into account when assessing the relationship between fish consumption and plasma phospholipid omega-3 FA levels.
文摘采用偏最小二乘法(Partial least squares,PLS)作为建模方法,对磷虾油近红外光谱的一阶微分(First-order difference,FD)、FD+SG(Savitzky-Golay,SG)滤波、FD+N(Norris,N)滤波、二阶微分(Second-order difference,SD)、SD+SG和SD+N等6种单一或复合方法进行处理,通过对不同方式处理后预测模型的交互验证均方根误差(Root mean square error of cross validation,RMSECV)、外部验证残差均方根(Root mean square error of external prediction,RMSEP)和外部验证用样品真实值的标准差(SD)与RMSEP的比值(The ratio of the RMSEP to standard deviation of reference data in the prediction,RPDEV)、建模相关系数(Correlation coefficient in calibration,RC)、交互验证相关系数(Correlation coefficient in cross validation,RCV)和外部验证相关系数(Correlation coefficient in external validation,REV)等参数比较,确定了磷虾油磷脂、EPA和DHA的近红外预测模型最佳处理方式为FD、FD和SD+N,酸价指标模型不需处理。在最优条件下,四种成分近红外预测模型的RC、REV和RCV,除了酸价的RCV略小(0.917)其余均达到0.95以上,同时,四种成分的RPDEV和RPDCV值,除酸价的RPDCV为2.365,略小于2.5,其余均符合大于2.5的要求,说明磷虾油磷脂、EPA和DHA的近红外预测模型预测准确度良好;RMSEC和RMSECV相差不大,说明模型稳定性较好。由于含量低、组成复杂等原因,磷虾油虾青素近红外检测模型的RC、RCV和REV均在0.60以下,说明近红外检测不适用于磷虾油中虾青素成分的快速检测。本文证实了近红外光谱技术可作为磷虾油中磷脂、EPA、DHA和酸价等主要指标的快速检测方法,是传统化学检测方法的有效替代和补充。
