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过表达NOLC1诱导人非小细胞肺癌细胞凋亡
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作者 杜亚兰 张茂盛 +5 位作者 王旌羽 张璐燕 周常博 佘晓双 郑方亮 朱春玉 《微生物学杂志》 CAS CSCD 北大核心 2024年第2期87-96,共10页
人核仁磷酸化蛋白1(Nucleolar and coiledbody phosphoprotein 1,NOLC1)在癌症的发生发展过程中起着至关重要的调控作用,为探讨NOLC1对肺癌细胞的作用,本研究通过Gateway系统构建重组NOLC1腺病毒载体,成功包装NOLC1腺病毒后,分别感染正... 人核仁磷酸化蛋白1(Nucleolar and coiledbody phosphoprotein 1,NOLC1)在癌症的发生发展过程中起着至关重要的调控作用,为探讨NOLC1对肺癌细胞的作用,本研究通过Gateway系统构建重组NOLC1腺病毒载体,成功包装NOLC1腺病毒后,分别感染正常人类胚胎肺细胞(HEL)和非小细胞肺癌细胞(A549细胞),过表达NOLC1。通过MTT实验、AnnexinV-APC/PI双染法和线粒体膜电位实验,证明与HEL细胞相比,NOLC1的过表达对A549细胞的活性降低、凋亡增加、线粒体膜电位下降影响较为显著;通过Real-time PCR检测Caspase家族、TNF与受体家族和BCL2家族基因的表达,发现过表达NOLC1明显上调了A549细胞中促凋亡基因的表达,下调了抗凋亡基因的表达,其中两种重要的促凋亡蛋白CASP8和BAX均显著上调,但是在HEL细胞中这种影响不明显。研究结果表明过表达NOLC1蛋白通过对线粒体通路和死亡受体通路的共同作用,对非小细胞癌具有显著的抗肿瘤活性。 展开更多
关键词 NOLC1(Nucleolar and coiledbody phosphoprotein 1) 肺癌细胞 腺病毒载体 过表达 细胞凋亡
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肺腺癌关键基因的筛选及分泌型磷酸蛋白1在肺腺癌中的表达
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作者 赵丹 王俊苹 +6 位作者 牟海军 王显艳 毕红霞 郑红艳 孔维丽 李晶 程薪樾 《当代医学》 2023年第34期21-29,共9页
目的基于美国国家生物技术信息中心(NCBI)基因表达数据库(GEO)和美国国家癌症研究所(NCI)癌症基因组图谱(TCGA)联合生物学信息分析筛选肺腺癌关键基因(HUB)及相关信号通路、分泌型磷酸蛋白1(SPP1)表达及与肺腺癌患者临床特征的相关性。... 目的基于美国国家生物技术信息中心(NCBI)基因表达数据库(GEO)和美国国家癌症研究所(NCI)癌症基因组图谱(TCGA)联合生物学信息分析筛选肺腺癌关键基因(HUB)及相关信号通路、分泌型磷酸蛋白1(SPP1)表达及与肺腺癌患者临床特征的相关性。方法从GEO数据库下载包括肺腺癌与正常或癌旁肺组织基因表达2个数据集GSE10072、GSE43458,收集2020年1月至2021年3月于齐齐哈尔医学院附属第三医院行手术治疗且病理明确诊断为肺腺癌患者的癌组织和癌旁标本作为肺腺癌临床验证数据集(QY3F)并进行标准化及处理,筛选出肺腺癌与正常肺组织之间的差异基因,并进行基因本体(GO)功能、京都基因与基因组百科全书(KEGG)通路富集分析;通过筛选出的差异基因构建蛋白质相互作用网络(PPI),分析并筛选HUB基因;通过TCGA数据集验证SPP1表达及与肺腺癌患者临床特征和预后的相关性;通过QY3F验证SPP1表达及与肺腺癌患者临床特征的相关性;通过TCGA数据库分析肺腺癌患者SPP1基因与相关通路的相关性。结果通过2个GEO数据集筛选出差异表达基因120个,上调表达23个,下调表达97个。GO功能富集分析结果显示,差异基因参与细胞外基质组织及细胞膜结构的形成和功能及调节细胞对生长因子刺激的反应、骨小梁形成、肽及糖胺聚糖绑定;KEGG通路富集分析结果显示,差异基因参与细胞外基质的黏附、蛋白质消化吸收及过氧化物酶体增殖物激活受体(PPAR)信号通路。筛选出前10的HUB基因分别为DNA拓扑异构酶Ⅱα(TOP2A)、基质金属蛋白酶9(MMP9)、SPP1、基质金属蛋白酶抑制剂1(TIMP1)、羟基类固醇17-β脱氢酶6(HSD17B6)、细胞周期蛋白B1(CCNB1)、尿激酶型纤维蛋白溶酶原激活因子(PLAU)、丝氨酸/苏氨酸蛋白激酶(PBK)、周期蛋白依赖激酶抑制因子3(CDKN3)和人Ⅰ型胶原α1(COL1A1)。10个HUB基因中,除HSD17B6在TCGA数据库肺腺癌患者癌组织中的表达下降外,其他基因表达均高于癌旁正常组织,差异有统计学意义(P<0.05),其中SPP1上调表达差异最明显[癌旁正常组织(4.02±1.57)vs.肺腺癌组织(8.49±2.08)(P<0.05)];SPP1表达与性别、年龄、种族、肿瘤大小、肿瘤远处转移、病理分期无相关性,但与肿瘤淋巴结转移相关(P<0.05),与预后呈负相关(P<0.05);ROC曲线分析结果显示,SPP1表达诊断肺腺癌的AUC为0.95。QY3F数据集分析结果显示,肺腺癌组织中SPP1表达水平明显高于癌旁正常组织(P<0.05)。SPP1表达与性别、年龄、肿瘤大小、TNM分期及病理分期无相关性。SPP1与上皮间质细胞转化(EMT)相关性最高(P<0.05)。结论SPP1在肺腺癌组织中呈明显高表达,与肿瘤淋巴转移相关,机制通路可能与EMT相关,且SPP1对肺腺癌诊断及预后判断具有一定价值,是肺腺癌发展的关键基因。 展开更多
关键词 肺腺癌 分泌型磷酸蛋白1 生物标志物 生物信息学 关键基因
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STIP1、HE4和CA125对卵巢癌的诊断(英文) 被引量:2
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作者 ADZABE Luckresse 蔡云朗 《东南大学学报(医学版)》 CAS 2014年第6期811-815,共5页
Although many tumor markers have been identified and studied in epithelial ovarian cancer,a potential and useful screening marker for ovarian cancer has not been yet clearly established. Ovarian cancer is considered a... Although many tumor markers have been identified and studied in epithelial ovarian cancer,a potential and useful screening marker for ovarian cancer has not been yet clearly established. Ovarian cancer is considered as a "silent killer"because of the absence of specific symptoms until late stage. Several validated biomarkers are currently used to diagnose and monitor the progression of the cancer,but very few of them show adequate specificity and sensitivity for different population screening. There is therefore an urge need to find biomarkers with high diagnostic accuracy and set up screening programs which can help detect ovarian cancer early,predict the response of the patient to anticancer therapy and guide physicians in choosing the best treatment for the patient. CA125,HE4 and STIP1 have been proven to play an important role as biomarkers in detecting and monitoring ovarian cancer. In this paper,we review evaluate,and highlight the role of CA125,HE4 and STIP1 in the detection of ovarian cancer.Potential biomarkers can help us distinguish malignancy from benign pelvic mass. 展开更多
关键词 ovarian cancer stress-induced phosphoprotein1 human epididymis protein4 cancer antigen 125 early diagnosis
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Phosphoprotein phosphatase 1-interacting proteins as therapeutic targets in prostate cancer
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作者 Juliana Felgueiras Margarida Fardilha 《World Journal of Pharmacology》 2014年第4期120-139,共20页
Prostate cancer is a major public health concern world-wide, being one of the most prevalent cancers in men. Great improvements have been made both in terms of early diagnosis and therapeutics. However, there is still... Prostate cancer is a major public health concern world-wide, being one of the most prevalent cancers in men. Great improvements have been made both in terms of early diagnosis and therapeutics. However, there is still an urgent need for reliable biomarkers that could overcome the lack of cancer-specifcity of prostate-specifc antigen, as well as alternative therapeutic targets for advanced metastatic cases. Reversible phosphorylation of proteins is a post-translational modifcation critical to the regulation of numerous cellular processes. Phosphoprotein phosphatase 1 (PPP1) is a major serine/threonine phos-phatase, whose specifcity is determined by its interacting proteins. These interactors can be PPP1 substrates, regulators, or even both. Deregulation of this protein-protein interaction network alters cell dynamics and underlies the development of several cancer hallmarks. Therefore, the identification of PPP1 interactome in specific cellular context is of crucial importance. The knowledge on PPP1 complexes in prostate cancer remains scarce, with only 4 holoenzymes characterized in human prostate cancer models. However, an increasing number of PPP1 interactors have been identifed as expressed in human prostate tissue, including the tumor suppressors TP53 and RB1. Efforts should be made in order to identify the role of such proteins in prostate carcinogenesis, since only 26 have yet well-recognized roles. Here, we revise literature and human protein databases to provide an in-depth knowledge on the biological significance of PPP1 complexes in human prostate carcinogenesis and their potential use as therapeutic targets for the development of new therapies for prostate cancer. 展开更多
关键词 Prostate cancer Reversible phosphorylation Phosphoprotein phosphatase 1 Phosphoprotein phosphatase 1-interacting proteins Protein complexes Therapeutic targets
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Phosphoprotein Phosphatase 1 Isoforms Alpha and Gamma Respond Differently to Prodigiosin Treatment and Present Alternative Kinase Targets in Melanoma Cells
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作者 Margarida Fardilha Joao Figueiredo +7 位作者 Margarita Espona-Fiedler Juliana Felgueiras Luis Korrodi-Gregorio Sara L.C.Esteves Sandra Rebelo Odete A.B.da Cruz Silva Edgar da Cruz e Silva Ricardo Perez-Tomas 《Journal of Biophysical Chemistry》 2014年第2期67-77,共11页
Reversible protein phosphorylation is a central regulatory mechanism of cell function. Deregulation of the balanced actions of protein kinases and phosphatases has been frequently associated with several pathological ... Reversible protein phosphorylation is a central regulatory mechanism of cell function. Deregulation of the balanced actions of protein kinases and phosphatases has been frequently associated with several pathological conditions, including cancer. Many studies have already addressed the role of protein kinases misregulation in cancer. However, much less is known about protein phosphatases influence. Phosphoprotein Phosphatase 1 (PPP1) is one of the major serine/threonine protein phosphatases who has three catalytic isoforms: PPP1CA, PPP1CB, and PPP1CC. Its function is achieved by binding to regulatory subunits, known as PPP1-interacting proteins (PIPs), which may prefer a catalytic isoform. Also, some inhibitors/enhancers may exhibit isoform specificity. Here we show that, prodigiosin (PG), a molecule with anticancer properties, promotes the formation of PPP1CA-AKT complex and not of PPP1CC-MAPK complex. Both, AKT and MAPK, are well-known PIPs from two pathways that crosstalk and regulate melanoma cells survival. In addition, the analysis performed using surface plasmon resonance (SPR) technology indicates that PPP1 interacts with obatoclax (OBX), a drug that belongs to the same family of PG. Overall, these results suggest that PG might, at least in part, act through PPP1C/PIPs. Also, this study is pioneer in demonstrating PPP1 isoform-specific modulation by small molecules. 展开更多
关键词 Phosphoprotein Phosphatase 1 Catalytic Subunit Surface Plasmon Resonance Mitogen-Activated Protein Kinase V-Akt Murine Thymoma Viral Oncogene Glycogen Synthase Kinase 3
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Cancer-associated fibroblast-derived secreted phosphoprotein 1 contributes to resistance of hepatocellular carcinoma to sorafenib and lenvatinib 被引量:8
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作者 JungWoo Eun Jung Hwan Yoon +12 位作者 Hye Ri Ahn Seokhwi Kim Young Bae Kim Su Bin Lim Won Park TaeWook Kang Geum Ok Baek Moon Gyeong Yoon Ju A Son Ji HyangWeon Soon Sun Kim Hyo Jung Cho Jae Youn Cheong 《Cancer Communications》 SCIE 2023年第4期455-479,共25页
Background:Cancer-associated fibroblasts(CAFs)play an important role in the induction of chemo-resistance.This study aimed to clarify the mechanism underlying CAF-mediated resistance to two tyrosine kinase inhibitors(... Background:Cancer-associated fibroblasts(CAFs)play an important role in the induction of chemo-resistance.This study aimed to clarify the mechanism underlying CAF-mediated resistance to two tyrosine kinase inhibitors(TKIs),sorafenib and lenvatinib,and to identify a novel therapeutic target for overcoming TKI resistance in hepatocellular carcinoma(HCC).Methods:We performed a systematic integrative analysis of publicly available gene expression datasets and whole-transcriptome sequencing data from 9 pairs of CAFs and para-cancer fibroblasts isolated from human HCC and para-tumor tissues,respectively,to identify key molecules that might induce resistance to TKIs.We then performed in vitro and in vivo experiments to validate selected targets and related mechanisms.The associations of plasma secreted phosphoprotein 1(SPP1)expression levels before sorafenib/lenvatinib treatment with progression-free survival(PFS)and overall survival(OS)of 54 patients with advanced HCC were evaluated using Kaplan-Meier and Cox regression analysis.Results:Bioinformatic analysis identified CAF-derived SPP1 as a candidate molecule driving TKI resistance.SPP1 inhibitors reversed CAF-induced TKI resistance in vitro and in vivo.CAF-derived SPP1 activated rapidly accelerated fibrosarcoma(RAF)/mitogen-activated protein kinase(MAPK)and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(mTOR)through the integrin-protein kinase C-alpha(PKCα)signaling pathway and promoted epithelial-to-mesenchymal transition(EMT).A high plasma SPP1 level before TKI treatment was identified as an independent predictor of poor PFS(P=0.026)and OS(P=0.047)in patients with advanced HCC after TKI treatment.Conclusions:CAF-derived SPP1 enhances TKI resistance in HCC via bypass activation of oncogenic signals and EMT promotion.Its inhibition represents a promising therapeutic strategy against TKI resistance inHCC.Moreover,plasma SPP1 level before TKI treatment represents a potential biomarker for treatment response prediction. 展开更多
关键词 drug resistance epithelial-to-mesenchymal transition hepatocellular carcinoma secreted phosphoprotein 1
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A high level of secreted phosphoprotein 1 is associated with macrophage infiltration and poor prognosis in hepatocellular carcinoma
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作者 Jianping Song Jingxian Sun +3 位作者 Shuhong Jing Tingxiao Zhang Jianlei Wang Yanfeng Liu 《iLIVER》 2023年第1期26-35,共10页
Background and aims:Secreted phosphoprotein 1(SPP1)functions in several physiological processes.The role of SPP1 expression in the prognosis and tumor immunity of hepatocellular carcinoma(HCC)is unknown.The aim of thi... Background and aims:Secreted phosphoprotein 1(SPP1)functions in several physiological processes.The role of SPP1 expression in the prognosis and tumor immunity of hepatocellular carcinoma(HCC)is unknown.The aim of this study was to investigate the expression pattern of SPP1 in HCC and its correlation with prognosis and tumor immunity.Methods:Clinical and gene expression data of The Cancer Genome Atlas-liver hepatocellular carcinoma(LIHC)cohort and 11 other HCC datasets were collected.The Kaplan–Meier method and Cox regression analysis were used to analyze the prognostic value of SPP1.The DESeq2 package in R was used to analyze SPP1-related genes.Gene Ontology analysis and gene set enrichment analysis were used to determine the biological function of SPP1 in HCC.The single sample Gene Set Enrichment Analysis(ssGSEA)method was used to analyze the immune infiltrates of HCC.Illumina human methylation 450 data and level 3 HTSeq-FPKM data from The Cancer Genome Atlas-LIHC were used to analyze the effects of DNA methylation level on SPP1 expression.Results:SPP1 was overexpressed in HCC and correlated with T stage,histological grade,adjacent hepatic tissue inflammation,and vascular invasion in HCC.The analysis of survival rates indicated that high SPP1 levels were associated with poor overall survival in HCC.Functional analysis showed that SPP1 is related to tumor immunity,especially macrophage infiltration.Aberrant demethylation of the promoter region is one of the mechanisms underlying the increase of SPP1 in HCC.Conclusion:Our results indicate that SPP1 is an independent prognostic factor for HCC and is correlated with the clinical features and macrophage infiltration in HCC. 展开更多
关键词 Hepatocellular carcinoma Secreted phosphoprotein 1 PROGNOSIS Promoter demethylation Tumor immunity
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Select nutrients and their effects on conceptus development in mammals 被引量:2
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作者 Fuller W.Bazer Xiaoqiu Wang +1 位作者 Greg A.Johnson Guoyao Wu 《Animal Nutrition》 SCIE 2015年第3期85-95,共11页
The dialogue between the mammalian conceptus(embryo/fetus and associated membranes) involves signaling for pregnancy recognition and maintenance of pregnancy during the critical peri-implantation period of pregnancy w... The dialogue between the mammalian conceptus(embryo/fetus and associated membranes) involves signaling for pregnancy recognition and maintenance of pregnancy during the critical peri-implantation period of pregnancy when the stage is set for implantation and placentation that precedes fetal development. Uterine epithelial cells secrete and/or transport a wide range of molecules, including nutrients,collectively referred to as histotroph that are transported into the fetal-placental vascular system to support growth and development of the conceptus. The availability of uterine-derived histotroph has long-term consequences for the health and well-being of the fetus and the prevention of adult onset of metabolic diseases. Histotroph includes numerous amino acids, but arginine plays a particularly important role as a source of nitric oxide and polyamines required for fetal-placental development in rodents, swine and humans through mechanisms that remain to be fully elucidated. Mechanisms whereby arginine regulates expression of genes via the mechanistic target of rapamycin cell signaling pathways critical to conceptus development, implantation and placentation are discussed in detail in this review. 展开更多
关键词 Amino acids Secreted phosphoprotein 1 Pregnancy Interferon tau Conceptus development TROPHECTODERM
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