[Objectives]To study the phosphorus removal performance of phosphate accumulating organisms(PAOs).[Methods]Activated sludge from domestic sewage treatment plant was used as the strain source,and phosphate accumulating...[Objectives]To study the phosphorus removal performance of phosphate accumulating organisms(PAOs).[Methods]Activated sludge from domestic sewage treatment plant was used as the strain source,and phosphate accumulating organisms were screened by plate streaking method and dilution coating plate method.Six kinds of excellent phosphate accumulating organisms were obtained by metachromatic granule staining experiment,total phosphorus experiment and simulated sewage phosphorus removal experiment to assist the observation of bac-terial morphology and experiment of phosphorus removal capacity.In addition,the influencing factors of phosphorus removal capacity(nitrogen source,trace metal ions)were analyzed.[Results]In the case of simulated sewage,the phosphorus removal rate of strain b was the highest,reaching 66.25%,while the phosphorus removal rate of strain e and f was about 10%lower than that of the phosphorus uptake experiment.[Conclusions]This study is expected to provide a theoretical reference for the gradual optimization of the screening method of phosphorus re-moval bacteria in domestic sewage treatment.展开更多
Filamentous bacteria(FB)overgrowth is an important cause of sludge bulking in wastewater treatment plants(WWTPs).However,to date,methods for the cultivation and preservation of isolated FB in the laboratory have not b...Filamentous bacteria(FB)overgrowth is an important cause of sludge bulking in wastewater treatment plants(WWTPs).However,to date,methods for the cultivation and preservation of isolated FB in the laboratory have not been completely described.Furthermore,research on whether FB can function as phosphorus accumulating organisms(PAOs)is limited.In this study,a pure strain,a Pseudomonas putida PAO-1(P.putida PAO-1)isolate with phosphorus removal functions was isolated from the biofilm of an alternating anaerobic/aerobic biofilter(AABF),and its physiological characteristics were studied.Nitrate or nitrite could be used by the strain P.putida PAO-1 as electron acceptors for denitrification during phosphorus anoxic uptake,and 0.63 mg NO-3-N was consumed to reduce 1 mg soluble orthophosphate(SOP)by P.putida PAO-1.The strain P.putida PAO-1 consumed phosphorus within the optimal pH range of 6 to 8 and the temperature range of 25℃to 35℃.Cell deformity was a main morphological trait of the strain P.putida PAO-1,and it could elongate(with an elongation rate of 300%-500%)when it was subjected to oligotrophic or high-salt stress(15 g·L-1 NaCl).The findings in this study provide a microbiological reference for understanding the special characteristics of a denitrifying PAO.展开更多
In this article, polyphosphate-accumulating organisms （PAOs） from a lab-scale continuous-flow two-sludge system was isolated and identified, the different phosphorus accumulation characteristics of the isolates unde...In this article, polyphosphate-accumulating organisms （PAOs） from a lab-scale continuous-flow two-sludge system was isolated and identified, the different phosphorus accumulation characteristics of the isolates under anoxic and aerobic conditions were investigated. Two kinds of PAOs were both found in the anoxic zones of the two-sludge system, one of them utilized only oxygen as electron aeceptor, and the other one utilized either nitrate or oxygen as electron aeceptor. Of the total eight isolates, five isolates were capable of utilizing both nitrate and oxygen as electron acceptors to uptake phosphorus to some extent. And three of the five isolates showed good phosphorus accumulative capacities both under anoxic or aerobic conditions, two identified as Alcaligenes and one identified as Pseudomonas. Streptococcus was observed weak anoxic phosphorus accumulation because of its weak denitrification capacity, but it showed good phosphorus accumulation capacity under aerobic conditions. One isolates identified as Enterobacteriaceae was proved to be a special species of PAOs, which could only uptake small amounts of phosphorus under anoxic conditions, although its denitrification capacity and aerobic phosphorus accumulation capacity were excellent.展开更多
Increasing attention has been paid to phosphate-accumulating organisms （PAOs） for their important role in biological phosphorus removal. In this study, microbial communities of PAOs cultivated under different carbon...Increasing attention has been paid to phosphate-accumulating organisms （PAOs） for their important role in biological phosphorus removal. In this study, microbial communities of PAOs cultivated under different carbon sources （sewage, glucose, and sodium acetate） were investigated and compared through culture-dependent and culture-independent methods, respectively. The results obtained using denaturing gradient gel electrophoresis （DGGE） of polymerase chain reaction-amplified 16S rDNA fragments revealed that the diversity of bacteria in a sewage-fed reactor （1#） was much higher than in a glucose-fed one （2#） and a sodium acetate-fed one （3#）; there were common PAOs in three reactors fed by different carbon sources. Five strains were separated from three systems by using a phosphaterich medium; they were from common bacteria isolated and three isolates could not be found in DGGE profile at all. Two isolates had good phosphorus removal ability. When the microbial diversity was studied, the molecular biological method was better than the culture-dependent one. When phosphorus removal characteristics were investigated, culture-dependent approach was more effective. Thus a combination of two methods is necessary to have a comprehensive view of PAOs.展开更多
A Pseudomonas strain(named as P.PAO-1) with phosphorous removal function was isolated and characterized.A new method of two-stage cultivation was applied to the strain to induce the synthesis of intracellular polyhydr...A Pseudomonas strain(named as P.PAO-1) with phosphorous removal function was isolated and characterized.A new method of two-stage cultivation was applied to the strain to induce the synthesis of intracellular polyhydroxylbutyrate(PHB) in the cells.In the first stage,bacterial cells were enriched under a high carbon source condition;in the second stage,the bacteria cells were cultivated under the nutrient-imbalanced conditions with the carbon source-regulated medium.As a result,the PHB content of strain P.PAO-1 reached 22.8% compared with the normal 7.41% in the non-acclimated strain.This change had led to the rising of P uptake from 1 to 25 mg·L^(-1).When added the strain P.PAO-1into the activated sludge(with the addition proportion of 1:1),the ratio of biological phosphorus removal increased by 14.9%under the normal alternating anaerobic/aerobic conditions with low-carbon consumption.The results demonstrated that the isolated pure culture strain P.PAO-1 belonged to the functional group of poly-P accumulating microorganism.When cultured by the two-stage cultivation method,the initial accumulation of PHB in the strain cells could be achieved and the phosphorous removal capacity of strain P.PAO-1 could be induced subsequently.When applied to the wastewater,strain P.PAO-1 performed phosphorus removal from the wastewater with or without the addition of activated sludge.展开更多
Performance of biological phosphorus removal in the oxic-settling-anaerobic(OSA) process was investigated. Cell staining and fluorescent in situ hybridization(FISH) were used to analyze characteristics and microbial c...Performance of biological phosphorus removal in the oxic-settling-anaerobic(OSA) process was investigated. Cell staining and fluorescent in situ hybridization(FISH) were used to analyze characteristics and microbial community of sludge. Experimental results showed that phosphorus removal efficiency was near 60% and the amount of biological phosphorus accumulation in aerobic sludge of the OSA system was up to 26.9 mg/g. Biological phosphorus removal efficiency was partially inhibited by carbon sources in the continuous OSA system. Contrasted to the OSA system,biological phosphorus removal efficiency was enhanced by 14% and the average total phosphorus(TP) contents of aerobic sludge were increased by 0.36 mg/g when sufficient carbon sources were supplied in batch experiments. Staining methods indicated that about 35% of microorganisms had typical characteristics of phosphorus accumulating organisms(PAOs) . FISH analysis demonstrated that PAOMIX-binding bacteria were predominant microbial communities in the OSA system,which accounted for around 28% of total bacteria.展开更多
旨在从环境样品中筛选对富营养化水体具有良好脱氮除磷效果的好氧反硝化菌。采集福州某养猪场污水处理池中的水样。通过反硝化细菌培养基培养、BTB培养基平板分离、硝酸盐还原试验和蓝白斑筛选法、异染颗粒以及聚-β-羟基丁酸(PHB)颗粒...旨在从环境样品中筛选对富营养化水体具有良好脱氮除磷效果的好氧反硝化菌。采集福州某养猪场污水处理池中的水样。通过反硝化细菌培养基培养、BTB培养基平板分离、硝酸盐还原试验和蓝白斑筛选法、异染颗粒以及聚-β-羟基丁酸(PHB)颗粒染色试验,筛选获得两株具有脱氮除磷特性的菌株,命名为N1和N2。经16S r RNA基因序列分析,N1和N2分别属于无色杆菌属(Achromobacter.sp)和短波单胞菌属(Brevundimonas.sp)。将菌株N1和N2复配,获得脱氮除磷复合菌FIM-1。考察了菌株对人工合成污水和富营养化水体脱氮除磷的效果。结果表明,两株菌在含磷量较低的水体中,对磷的去除率较高,相对于单菌,复合菌表现出更佳的脱氮除磷效果。展开更多
In order to further explore the mechanism of denitrifying phosphorus removal,the growth characteristics and denitrifying phosphorus removal characteristics of a typical denitrifying phosphate accumulating bacteria(DPB...In order to further explore the mechanism of denitrifying phosphorus removal,the growth characteristics and denitrifying phosphorus removal characteristics of a typical denitrifying phosphate accumulating bacteria(DPB) which was isolated by previous experiment were studied.The effect of different electron acceptors on the efficiency of nitrogen and phosphorus removal was investigated by pure bacteria and reactor statics simulation tests.The growth curve of the DPB was typical,and its incubation period was less than 1 h and the logarithmic phase about 14 h.The result showed that the stain could utilize both nitrite and nitrate as different electron acceptors to take up phosphorus.And there was a good linear correlation between P removal and NO-3-N removal during phosphorus removal process.The sensitivity to nitrite of the strain was low.The effect of electron acceptors on phosphorus removal was not significant.The efficiency of removal for phosphorus and the nitrate was both above 60%,and that for nitrite was 100%.展开更多
基金Supported by Zhaoqing University Innovation and Entrepreneurship Training Program for College Students(202310580018).
文摘[Objectives]To study the phosphorus removal performance of phosphate accumulating organisms(PAOs).[Methods]Activated sludge from domestic sewage treatment plant was used as the strain source,and phosphate accumulating organisms were screened by plate streaking method and dilution coating plate method.Six kinds of excellent phosphate accumulating organisms were obtained by metachromatic granule staining experiment,total phosphorus experiment and simulated sewage phosphorus removal experiment to assist the observation of bac-terial morphology and experiment of phosphorus removal capacity.In addition,the influencing factors of phosphorus removal capacity(nitrogen source,trace metal ions)were analyzed.[Results]In the case of simulated sewage,the phosphorus removal rate of strain b was the highest,reaching 66.25%,while the phosphorus removal rate of strain e and f was about 10%lower than that of the phosphorus uptake experiment.[Conclusions]This study is expected to provide a theoretical reference for the gradual optimization of the screening method of phosphorus re-moval bacteria in domestic sewage treatment.
基金National Natural Science Foundation of China(No.21777024)National Key Research and Development Project,China(No.2019YFC0408503)。
文摘Filamentous bacteria(FB)overgrowth is an important cause of sludge bulking in wastewater treatment plants(WWTPs).However,to date,methods for the cultivation and preservation of isolated FB in the laboratory have not been completely described.Furthermore,research on whether FB can function as phosphorus accumulating organisms(PAOs)is limited.In this study,a pure strain,a Pseudomonas putida PAO-1(P.putida PAO-1)isolate with phosphorus removal functions was isolated from the biofilm of an alternating anaerobic/aerobic biofilter(AABF),and its physiological characteristics were studied.Nitrate or nitrite could be used by the strain P.putida PAO-1 as electron acceptors for denitrification during phosphorus anoxic uptake,and 0.63 mg NO-3-N was consumed to reduce 1 mg soluble orthophosphate(SOP)by P.putida PAO-1.The strain P.putida PAO-1 consumed phosphorus within the optimal pH range of 6 to 8 and the temperature range of 25℃to 35℃.Cell deformity was a main morphological trait of the strain P.putida PAO-1,and it could elongate(with an elongation rate of 300%-500%)when it was subjected to oligotrophic or high-salt stress(15 g·L-1 NaCl).The findings in this study provide a microbiological reference for understanding the special characteristics of a denitrifying PAO.
基金Project supported by the Science and Technology Project of Heilongjiang Province (No. GA01C201-03).
文摘In this article, polyphosphate-accumulating organisms （PAOs） from a lab-scale continuous-flow two-sludge system was isolated and identified, the different phosphorus accumulation characteristics of the isolates under anoxic and aerobic conditions were investigated. Two kinds of PAOs were both found in the anoxic zones of the two-sludge system, one of them utilized only oxygen as electron aeceptor, and the other one utilized either nitrate or oxygen as electron aeceptor. Of the total eight isolates, five isolates were capable of utilizing both nitrate and oxygen as electron acceptors to uptake phosphorus to some extent. And three of the five isolates showed good phosphorus accumulative capacities both under anoxic or aerobic conditions, two identified as Alcaligenes and one identified as Pseudomonas. Streptococcus was observed weak anoxic phosphorus accumulation because of its weak denitrification capacity, but it showed good phosphorus accumulation capacity under aerobic conditions. One isolates identified as Enterobacteriaceae was proved to be a special species of PAOs, which could only uptake small amounts of phosphorus under anoxic conditions, although its denitrification capacity and aerobic phosphorus accumulation capacity were excellent.
基金Project supported by the Young Teacher Foundation of Donghua University(No.113-10-0044065)Key(keygrant)Project of Chinese Ministry of Education(No.107046)Program for New Century Excellent Talents in University(NCET)
文摘Increasing attention has been paid to phosphate-accumulating organisms （PAOs） for their important role in biological phosphorus removal. In this study, microbial communities of PAOs cultivated under different carbon sources （sewage, glucose, and sodium acetate） were investigated and compared through culture-dependent and culture-independent methods, respectively. The results obtained using denaturing gradient gel electrophoresis （DGGE） of polymerase chain reaction-amplified 16S rDNA fragments revealed that the diversity of bacteria in a sewage-fed reactor （1#） was much higher than in a glucose-fed one （2#） and a sodium acetate-fed one （3#）; there were common PAOs in three reactors fed by different carbon sources. Five strains were separated from three systems by using a phosphaterich medium; they were from common bacteria isolated and three isolates could not be found in DGGE profile at all. Two isolates had good phosphorus removal ability. When the microbial diversity was studied, the molecular biological method was better than the culture-dependent one. When phosphorus removal characteristics were investigated, culture-dependent approach was more effective. Thus a combination of two methods is necessary to have a comprehensive view of PAOs.
基金National Natural Science Foundation of China(No.51478099)Scientific Research Foundation for Returned Overseas Chinese Scholars,China(No.SEM-11W11329)Natural Science Foundation of Shanghai,China(No.16ZR1402000)
文摘A Pseudomonas strain(named as P.PAO-1) with phosphorous removal function was isolated and characterized.A new method of two-stage cultivation was applied to the strain to induce the synthesis of intracellular polyhydroxylbutyrate(PHB) in the cells.In the first stage,bacterial cells were enriched under a high carbon source condition;in the second stage,the bacteria cells were cultivated under the nutrient-imbalanced conditions with the carbon source-regulated medium.As a result,the PHB content of strain P.PAO-1 reached 22.8% compared with the normal 7.41% in the non-acclimated strain.This change had led to the rising of P uptake from 1 to 25 mg·L^(-1).When added the strain P.PAO-1into the activated sludge(with the addition proportion of 1:1),the ratio of biological phosphorus removal increased by 14.9%under the normal alternating anaerobic/aerobic conditions with low-carbon consumption.The results demonstrated that the isolated pure culture strain P.PAO-1 belonged to the functional group of poly-P accumulating microorganism.When cultured by the two-stage cultivation method,the initial accumulation of PHB in the strain cells could be achieved and the phosphorous removal capacity of strain P.PAO-1 could be induced subsequently.When applied to the wastewater,strain P.PAO-1 performed phosphorus removal from the wastewater with or without the addition of activated sludge.
基金Project (No. 2006BAC19B04) supported by the National Key Technology R&D Program of China
文摘Performance of biological phosphorus removal in the oxic-settling-anaerobic(OSA) process was investigated. Cell staining and fluorescent in situ hybridization(FISH) were used to analyze characteristics and microbial community of sludge. Experimental results showed that phosphorus removal efficiency was near 60% and the amount of biological phosphorus accumulation in aerobic sludge of the OSA system was up to 26.9 mg/g. Biological phosphorus removal efficiency was partially inhibited by carbon sources in the continuous OSA system. Contrasted to the OSA system,biological phosphorus removal efficiency was enhanced by 14% and the average total phosphorus(TP) contents of aerobic sludge were increased by 0.36 mg/g when sufficient carbon sources were supplied in batch experiments. Staining methods indicated that about 35% of microorganisms had typical characteristics of phosphorus accumulating organisms(PAOs) . FISH analysis demonstrated that PAOMIX-binding bacteria were predominant microbial communities in the OSA system,which accounted for around 28% of total bacteria.
文摘旨在从环境样品中筛选对富营养化水体具有良好脱氮除磷效果的好氧反硝化菌。采集福州某养猪场污水处理池中的水样。通过反硝化细菌培养基培养、BTB培养基平板分离、硝酸盐还原试验和蓝白斑筛选法、异染颗粒以及聚-β-羟基丁酸(PHB)颗粒染色试验,筛选获得两株具有脱氮除磷特性的菌株,命名为N1和N2。经16S r RNA基因序列分析,N1和N2分别属于无色杆菌属(Achromobacter.sp)和短波单胞菌属(Brevundimonas.sp)。将菌株N1和N2复配,获得脱氮除磷复合菌FIM-1。考察了菌株对人工合成污水和富营养化水体脱氮除磷的效果。结果表明,两株菌在含磷量较低的水体中,对磷的去除率较高,相对于单菌,复合菌表现出更佳的脱氮除磷效果。
文摘In order to further explore the mechanism of denitrifying phosphorus removal,the growth characteristics and denitrifying phosphorus removal characteristics of a typical denitrifying phosphate accumulating bacteria(DPB) which was isolated by previous experiment were studied.The effect of different electron acceptors on the efficiency of nitrogen and phosphorus removal was investigated by pure bacteria and reactor statics simulation tests.The growth curve of the DPB was typical,and its incubation period was less than 1 h and the logarithmic phase about 14 h.The result showed that the stain could utilize both nitrite and nitrate as different electron acceptors to take up phosphorus.And there was a good linear correlation between P removal and NO-3-N removal during phosphorus removal process.The sensitivity to nitrite of the strain was low.The effect of electron acceptors on phosphorus removal was not significant.The efficiency of removal for phosphorus and the nitrate was both above 60%,and that for nitrite was 100%.
