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Ge and Cu recovery from precipitating vitriol supernatant in zinc plant 被引量:3
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作者 周兆安 楚广 +2 位作者 甘红祥 杨天足 陈霖 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2013年第5期1506-1511,共6页
A novel technology using Fe powder as reducing agent for Ge and Cu recovery from precipitating vitriol supernatant in Zn hydrometallurgical plant was investigated. The results show that reaction time, temperature, agi... A novel technology using Fe powder as reducing agent for Ge and Cu recovery from precipitating vitriol supernatant in Zn hydrometallurgical plant was investigated. The results show that reaction time, temperature, agitation speed, initial pH value of solution and the amount of reducing Fe have significant effects on recovering Ge and Cu, and the optimum process operating parameters are established as follows: time 120 min, initial pH value 1.5, the dosage of reducing Fe powder 4 g/L, agitation speed 600 r/min and temperature 80 °C. Under these experimental conditions, the recovery ratios of Ge and Cu from precipitating vitriol supernatant in Zn hydrometallurgical plant can reach 96% and 100%, respectively. The content of Ge in the reduced residue reaches up to 2.06% (mass fraction), indicating that the separation and enrichment of Ge from the Zn sulfate solution is realized. The grade of Ge and Cu can reach up to 4.88% and 56.75%, respectively, when the reduced residue is further processed. 展开更多
关键词 RECOVERY Ge CU zinc hydrometallurgy precipitating vitriol supernatant CEMENTATION Fe powder
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Effect of Lactobacillus rhamnosus GG supernatant on serotonin transporter expression in rats with post-infectious irritable bowel syndrome 被引量:10
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作者 Ya-nan Cao Li-juan Feng +7 位作者 Yuan-Yuan Liu Kui jiang Mao-jun Zhang Yi-xin Gu Bang-Mao Wang jia Gao Ze-Lan Wang Yu-Ming Wang 《World Journal of Gastroenterology》 SCIE CAS 2018年第3期338-350,共13页
AIM To evaluate the effect of Lactobacillus rhamnosus GG supernatant(LGG-s) on the expression of serotonin transporter(SERT) in rats with post-infectious irritable bowel syndrome(PI-IBS).METHODS Campylobacter jejuni 8... AIM To evaluate the effect of Lactobacillus rhamnosus GG supernatant(LGG-s) on the expression of serotonin transporter(SERT) in rats with post-infectious irritable bowel syndrome(PI-IBS).METHODS Campylobacter jejuni 81-176(1010 CFU/m L) was used to induce intestinal infection to develop a PI-IBS model. After evaluation of the post-infectious phase by biochemical tests, Dn A agarose gel electrophoresis, abdominal withdrawal reflex(AWR) test, and the intestinal motility test, four PI-IBS groups received different concentrations of LGG-s for 4 wk. The treatments were maintained for 1.0, 2.0, 3.0 or 4.0 wk during the experiment, and the colons and brains were removed for later use each week. SERT m Rn A and protein levels were detected by real-time PCR and Western blot, respectively.RESULTS The levels of SERT m Rn A and protein in intestinal tissue were higher in rats treated with LGG-s than in control rats and PI-IBS rats gavaged with PBS during the whole study. Undiluted LGG-s up-regulated SERT m Rn A level by 2.67 times compared with the control group by week 2, and SERT m Rn A expression kept increasing later. Double-diluted LGG-s was similar to undiluted-LGG-s, resulting in high levels of SERT m Rn A. Triple-diluted LGG-s up-regulated SERT m Rn A expression level by 6.9-times compared with the control group, but SERT m Rn A expression decreased rapidly at the end of the second week. At the first week, SERT protein levels were basically comparable in rats treated with undiluted LGG-s, double-diluted LGG-s, and triplediluted LGG-s, which were higher than those in the control group and PBS-treated PI-IBS group. SERT protein levels in the intestine were also comparable in rats treated with undiluted LGG-s, double-diluted LGG-s, and triple-diluted LGG-s by the second and third weeks. SERT m Rn A and protein levels in the brain had no statistical difference in the groups during the experiment.CONCLUSION LGG-s can up-regulate SERT m Rn A and protein levels in intestinal tissue but has no influence in brain tissue in rats with PI-IBS. 展开更多
关键词 SEROTONIN TRANSPORTER intestinal infection LACTOBACILLUS rhamnosus supernatant IRRITABLE BOWEL syndrome
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Effect of Bacillus subtilis,Enterococcus faecium,and Enterococcus faecalis supernatants on serotonin transporter expression in cells and tissues 被引量:2
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作者 Yi-Ming Chen Ying Li +8 位作者 Xin Wang Ze-Lan Wang Jun-Jie Hou Shuai Su Wei-Long Zhong Xin Xu Jie Zhang Bang-Mao Wang Yu-Ming Wang 《World Journal of Gastroenterology》 SCIE CAS 2022年第5期532-546,共15页
BACKGROUND Bacillus subtilis(B.subtilis),Enterococcus faecium(E.faecium),and Enterococcus faecalis(E.faecalis)are probiotics that are widely used in the clinical treatment of irritable bowel syndrome(IBS).Whether the ... BACKGROUND Bacillus subtilis(B.subtilis),Enterococcus faecium(E.faecium),and Enterococcus faecalis(E.faecalis)are probiotics that are widely used in the clinical treatment of irritable bowel syndrome(IBS).Whether the supernatants of these three probiotics can improve gastrointestinal sensation and movement by regulating the serotonin transporter(SERT)expression needs to be clarified.AIM To investigate whether B.subtilis,E.faecium,and E.faecalis supernatants can upregulate SERT expression in vitro and in vivo.METHODS Caco-2 and HT-29 cells were stimulated with probiotic culture supernatants for 12 and 24 h,respectively.A male Sprague-Dawley rat model of post-infectious irritable bowel syndrome(PI-IBS)was established and the rats were treated with phosphate-buffered saline(group A)and three probiotics culture supernatants(groups B,C,and D)for 4 wk.The levels of SERT were detected by quantitative PCR and western blotting.RESULTS The levels of SERT at post-treatment 12 and 24 h were significantly elevated in Caco-2 cells treated with B.subtilis supernatant compared with those in the control group(aP<0.05).Those levels were markedly upregulated in Caco-2 cells stimulated with E.faecium and E.faecalis supernatants at 24 h(aP<0.05).In addition,SERT expression in groups B,C,and D was significantly higher than that in group A in the 2nd wk(aP<0.05).Increased SERT expression was only found in group D in the 3rd wk(aP<0.05).However,there was no significant difference in SERT expression between the groups in the last week(P>0.05).CONCLUSION The supernatants of B.subtilis,E.faecium,and E.faecalis can upregulate SERT expression in intestinal epithelial cells and the intestinal tissues in the rat model of PI-IBS. 展开更多
关键词 Bacillus subtilis supernatant Enterococcus faecalis supernatant Enterococcus faecium supernatant Serotonin transporter expression Irritable bowel syndrome
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Bioactivities of Culture Supernatants from Retroviral Packaging Cells Carrying the Mouse Fas Ligand Gene 被引量:1
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作者 刘凌波 邹萍 +2 位作者 郭荣 肖娟 徐之良 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2001年第3期215-218,共4页
The bioactivities of culture supernatants from retroviral packaging cells carrying the mouse Fas ligand (mFasL) gene was investigated. FasLcDNA was cloned into PLXIN with an internal ribosome entry site to link two ci... The bioactivities of culture supernatants from retroviral packaging cells carrying the mouse Fas ligand (mFasL) gene was investigated. FasLcDNA was cloned into PLXIN with an internal ribosome entry site to link two cistrons through gene recombination technology, PLXIN and the recombinant vector PLFIN were separately transfected into PA317 retrovirus packing cell line by lipofectamine 2000, and the resistant clones were selected with G418 selective medium. The integration of genome DNA was assayed by genomic DNA PCR. NIH3T3 cells were transduced by the culture supernatants from PA317 carrying the mFasLcDNA gene, and were selected with G418 selective medium, so as to select the PLFIN-PA317 clone capable of producing higher titer of supernatants. The levels of mFasL protein on NIH3T3 cells membrane were assayed by flow cytometry (FCM). The biological activity of mFasL on NIH3T3 cells membrane was investigated by the inducing apoptosis of Fas + Yac-1 cells co-cultured with NIH3T3 cells expressing Fas ligand. To explore the direct mFasL cytotoxicity of culture supernatants from retroviral packaging cells carrying the mFasL gene, the culture supernatants from PLFIN-PA317 and PLXIN-PA317 were separately co-cultured with Yac-1 cells in parallel. The recombinant PLFIN was successfully constructed. The highest titer of supernatants from twelve resistant clones was 8.5×10 5 colony-forming-unit (CFU)/ml. The NIH3T3 cells transfected by above supernatants had a higher level of mFasL (53.81±6.9 %), and significantly induced the apoptosis of Fas + Yac-1 cells (56.78±4.5 %), as both were cocultured for 5 h at 1∶1 ratio, whereas it is 7.08±3.4 % in control group (P<0.01). Supernatant from PLFIN-PA317 could also directly induce the apoptosis of Yac-1 within 5 h of incubation. Thus, the culture supernatants from PLFIN-PA317 possessed both infectivity and cytotoxicity of mFasL. 展开更多
关键词 mFasL retroviral packing cell culture supernatant bioactivity
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Rheological properties in supernatant of peach gum from almond(Prunus dulcis)
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作者 王森 谢碧霞 +1 位作者 钟秋平 杜红岩 《Journal of Central South University》 SCIE EI CAS 2008年第S1期509-515,共7页
The rheological properties in the supernatant of peach gum from Prunnus dulcis were discussed in order to provide more scientific technical parameters and references for developing peach gum as a kind of medicinal gum... The rheological properties in the supernatant of peach gum from Prunnus dulcis were discussed in order to provide more scientific technical parameters and references for developing peach gum as a kind of medicinal gum.The rheological properties in the supernatant of peach gum were comparatively studied in different material ratios,temperatures,shaking times,pH values and salinities.The results show that,1) the mathematical model of shear rate with material ratio and shear stress is Y=0.069X12+0.035X2 -1.174,R2=0.942;2) the mathematical model of shear rate with temperature and shear stress is Y=4.936X12+0.023 2X2-1.688,R2=0.937;3) the mathematical model of shear rate with shaking time and shear stress is Y=0.005 192 X13-0.140 73X12+1.249 045X1+ 0.036 546 X2-3.644 29,R2=0.954 3;4) the effects of pH value on the rheological properties in the supernatant of peach gum are comparatively complicated with a varying range of 3-11 and the shear rate shows a change trend of saddle model;5) the mathematical model of shear rate with the concentration of NaCl and shear stress is Y=-0.037 44X1+0.012 93 X2,R2=0.998;6) the mathematical model of shear rate with the concentration of CaCl2 and shear stress is Y=0.025 789X1+0.016 19X2,R2 =0.999;and 7) the mathematical model of shear rate with the concentration of sorbic acid potassium and shear stress is Y=0.079 5X1+0.017 3X2,R2=0.998.The results show that the material ratio,temperature,shaking time,pH value significantly affect the rheological properties in the supernatant of peach gum,and the concentrations of NaCl and CaCl2 also significantly affect the rheological properties expect the concentration of sorbic acid potassium. 展开更多
关键词 PEACH gum supernatant RHEOLOGICAL PROPERTY
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PROLIFERATION OF ANTI-CD_3 McAb AND IL-2 INDUCED SPLENOCYTES AND ANTITUMOR EFFECT OF THEIR CULTURE SUPERNATANTS
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作者 沈关心 王晓林 +2 位作者 朱慧芬 张悦 邵静芳 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1995年第1期15-19,共5页
The proliferation of splenocytes from health adults was induced by anti-CD3 McAb and IL-2.The proliferative potential of the splenocytes and antitumor activity of their culture supernatants of splenocytes were studie... The proliferation of splenocytes from health adults was induced by anti-CD3 McAb and IL-2.The proliferative potential of the splenocytes and antitumor activity of their culture supernatants of splenocytes were studied.The results showed that anti-CD3 McAb not only enhanced the proliferation of the splenocytes directly,but also enhanceil that of induced by IL-2.Their enhancing effect was more significant when the incubation time in vitro was prolonged.The culture supernatants of anti-CD3 and IL-2 induced splenocytes also had the antitumor activity and enhancing capability to the antitumor activity of LAK tells.The results suggested that LAK cells could secret lymphokine,and this effect would be synergically promoted when anti-CD3 and IL-2 were simultaneously used. 展开更多
关键词 Anti-CD_3 McAb IL-2 SPLENOCYTES Cultured supernatants.
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Antifungal Activity against <i>Aspergillus parasiticus</i>of Supernatants from Whey Permeates Fermented with Kefir Grains
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作者 Raúl Ricardo Gamba Carolina Ni Colo +4 位作者 Mariana Correa Andrea Astoreca Teresa Alconada Graciela De Antoni Angela León Peláez 《Advances in Microbiology》 2015年第6期479-492,共14页
Aspergillus parasiticus, a common fungal contaminant in food, produces aflatoxin B1, which is classified as human carcinogen. Kefir is an ancient fermented beverage obtained by the fermentation of different substrates... Aspergillus parasiticus, a common fungal contaminant in food, produces aflatoxin B1, which is classified as human carcinogen. Kefir is an ancient fermented beverage obtained by the fermentation of different substrates with kefir grains. A very important waste produced by the dairy cheese industry is the whey permeate, which nowadays is a strong ambient contaminant. The aim of this work was to assess the effect of whey permeates fermented with kefir grains against A. parasiticus growth, aflatoxin B1 biosynthesis, and the kefir microorganisms protection against the cell damage produced by aflatoxin B1. It was observed that kefir-cell-free-supernatants (CFS) produced fungal inhibition. A fungicidal effect was observed with 65% v/v of CFS in the culture medium (final pH 4.55 and total undissociated lactic and acetic acid concentration 34.08 mM). Under these conditions, aflatoxin production was not detected. Finally, it was found that non-viable kefir microorganisms protected HepG2 cells from the damage produced by aflatoxin B1. 展开更多
关键词 Aspergillus PARASITICUS WHEY Permeate Aflatoxin B1 KEFIR supernatants Lactic ACID Acetic ACID Cytotoxicity HepG2 Cells
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Phosphorus Recovery as Struvite from the Supernatant of Anaerobic Digestion in Wastewater Treatment Plant
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作者 Gang Xue Yanan Liu Yanqun Wang 《Journal of Environmental Science and Engineering》 2010年第1期14-19,共6页
Phosphorus is an irreplaceable and depletable element. Furthermore, it has an almost one-way circulation on earth, so it is necessary to close the phosphorus cycle loop. Phosphorus could be recovered as struvite, whic... Phosphorus is an irreplaceable and depletable element. Furthermore, it has an almost one-way circulation on earth, so it is necessary to close the phosphorus cycle loop. Phosphorus could be recovered as struvite, which is a good slow-released fertilizer for agriculture. The supernatant of anaerobic digestion used to treat sludge from wastewater treatment plant is one main source from which phosphorus can be recovered. Studies have proven that phosphorus recovery from digester supematant is a feasible choice to preserve phosphorus rock technically and economically. A modified "PHOSNIX" P-recovery process was applied under the operating conditions of a 9.0 pH value and a 1.8 mg: P ratio with the influent of the centrate coming from the sludge centrifuge of the Songjiang Wastewater Treatment Plant in Shanghai. More than 80% influent phosphorus was recovered as struvite. Crystal products with good purity and low heavy metal content were gained. The largest crystal had a length of up to 0.26 mm. It was found in our study that the reaction time did not play an important role in crystal growth. Therefore, the optimization of the reaction condition for crystal growth should be examined in future study. 展开更多
关键词 Phosphorus recovery STRUVITE supernatant anaerobic digestion wastewater treatment plant.
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冠突散囊菌Ec-12上清抑制小鼠的伤寒沙门菌机制的初步分析
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作者 李兆龙 孔祥瑞 +4 位作者 林锋强 王秀萍 赵冉 彭小莉 陈常颂 《畜牧兽医学报》 CAS CSCD 北大核心 2024年第2期739-750,共12页
本研究旨在筛选黑茶中有益的冠突散囊菌(Eurotium cristatum,EC)并揭示该菌上清抑制鼠伤寒沙门菌(Salmonella Typhimurium)的初步机制。采用稀释涂布法分离冠突散囊菌;通过形态学和ITS rRNA基因序列分析其分类地位;采用生长速率法分析... 本研究旨在筛选黑茶中有益的冠突散囊菌(Eurotium cristatum,EC)并揭示该菌上清抑制鼠伤寒沙门菌(Salmonella Typhimurium)的初步机制。采用稀释涂布法分离冠突散囊菌;通过形态学和ITS rRNA基因序列分析其分类地位;采用生长速率法分析其增殖条件及增殖的稳定性;采用琼脂扩散抑菌试验、耐酸碱、耐热及紫外光稳定性试验、电镜观察及小鼠动物试验等以评估该Ec-12上清抑制鼠伤寒沙门菌的初步机制。结果显示:筛选到一株有较强抑制鼠伤寒沙门菌作用的冠突散囊菌Ec-12,抑菌圈分别为10.4 mm±1.5 mm。根据形态学、生长特征及ITS rRNA基因序列分析,将其鉴定为冠突散囊菌,并命名菌株Ec-12。粗分离的菌株Ec-12上清在pH近中性条件产生较好的抑菌效果,抑菌稳定好。经电镜观察可见粗分离菌株Ec-12上清导致鼠伤寒沙门菌的菌体变形,表层粗糙等抑菌现象。并明显降低小鼠感染鼠伤寒沙门菌引起的腹泻率和死亡率,提高小鼠绒毛的完整度和增加黏液蛋白的分泌(P<0.05),减少结肠细胞的凋亡(P<0.05),抑制鼠伤寒沙门菌对小鼠的危害。冠突散囊菌Ec-12菌株上清作用于鼠胃肠道内的鼠伤寒沙门菌,破坏了鼠伤寒沙门菌结构完整性,同时又作用于鼠的肠道,提高肠绒毛完整性,促进结肠黏液蛋白的分泌,减少结肠细胞的凋亡,进而降低鼠伤寒沙门菌引起的腹泻及死亡,它具备一定益生的潜力。 展开更多
关键词 冠突散囊菌 上清 鼠伤寒沙门菌 抑制
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凝结芽孢杆菌培养液及其离心上清液、菌悬液的抑菌性能研究
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作者 王雪艳 王乙茹 +5 位作者 黄遵锡 陈德近 周安树 何雪 安清聪 程志斌 《饲料工业》 CAS 北大核心 2024年第6期111-117,共7页
试验旨在比较研究凝结芽孢杆菌培养液(B.coagulans culture solution,BCS)及其离心上清液(centrifuged supernatant of B.coagulans culture solution,CSS)、菌悬液(centrifuged bacterial suspension of B.coagulans culture solution,... 试验旨在比较研究凝结芽孢杆菌培养液(B.coagulans culture solution,BCS)及其离心上清液(centrifuged supernatant of B.coagulans culture solution,CSS)、菌悬液(centrifuged bacterial suspension of B.coagulans culture solution,CBS)在不同pH条件下的抑菌性能差异。试验设计4个处理组:无菌蒸馏水组(sterile distilled water,SDW组,对照组)、BCS组、CSS组、CBS组。采用牛津杯体外抑菌试验法,参照畜禽胃肠道pH,检测4组在不同pH(2.5、4.0、5.5、7.0)条件下,对3个肠道有害菌(猪霍乱沙门氏菌、鸡白痢沙门氏菌、大肠杆菌K88)的抑菌圈值,统计比较抑菌性能的差异。模拟肠道pH环境的结果显示:pH 4.0、5.5、7.0条件下,BCS组和CBS组对3个指示菌均有抑制作用,且CBS组抑制效果显著大于BCS组(P<0.05);CSS组在pH 4.0条件下,对3个指示菌有抑制作用,但在pH 5.5和7.0时均无抑制作用;SDW组在pH 4.0、5.5和7.0时,对3个指示菌均无抑制作用。模拟胃pH环境的结果显示:pH 2.5条件下,4个处理组均对3个指示菌有强抑制作用,BCS、CSS、CBS 3组之间抑制效果差异不显著(P>0.05),均显著大于SDW组(P<0.05)。以上结果表明,凝结芽孢杆菌的培养液(BCS)及其离心菌悬液(CBS)可用于牛津杯抑菌试验操作,能反映菌株的抑菌性能;相反,离心上清液(CSS)的抑菌效果较弱,不适合凝结芽孢杆菌的体外抑菌试验操作。此外,本次体外抑菌研究支持了凝结芽孢杆菌在肠道环境对有害菌的有效抑制作用不受肠道pH的影响。 展开更多
关键词 凝结芽孢杆菌 培养液 离心上清液 菌悬液 抑菌性能 体外牛津杯抑菌法
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枯草芽孢杆菌无细胞上清液抑制单增李斯特菌生物被膜形成的研究 被引量:2
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作者 胡安祺 杨慧轩 +5 位作者 姚现琦 董鹏程 朱立贤 罗欣 张一敏 刘昀阁 《食品与发酵工业》 CAS CSCD 北大核心 2024年第13期1-8,共8页
单增李斯特菌(Listeria monocytogenes)是一种常见的食源性致病菌,其生物被膜是导致食品污染和疾病传播的重要原因。该研究选用枯草芽孢杆菌(Bacillus subtilis)无细胞上清液(cell free supernatant,CFS)作为抑制剂,探究B.subtilis CFS... 单增李斯特菌(Listeria monocytogenes)是一种常见的食源性致病菌,其生物被膜是导致食品污染和疾病传播的重要原因。该研究选用枯草芽孢杆菌(Bacillus subtilis)无细胞上清液(cell free supernatant,CFS)作为抑制剂,探究B.subtilis CFS在抑制L.monocytogenes野生菌株118(以下简称No.118)生物被膜方面的作用和潜力。首先测定了B.subtilis CFS的最小抑菌浓度(minimum inhibitory concentration,MIC),接着采用结晶紫染色法测定了不同亚最小抑菌浓度下B.subtilis CFS对No.118的生物被膜抑制率,发现不同亚抑菌浓度下的CFS对No.118生物被膜均具有显著的抑制作用,且在1/64 MIC时生物被膜抑制率仍可达到47%;同时B.subtilis CFS对No.118生物被膜代谢活性、自聚集率、表面疏水性、群集泳动能力和膜外聚合物分泌也均有显著抑制作用;最后,通过激光共聚焦显微镜观察了在CFS作用下,No.118生物被膜结构和细胞分布的变化规律,经CFS处理后,No.118生物被膜结构松散,细菌数量明显减少。综上,B.subtilis CFS可通过影响No.118生物被膜细胞的代谢活性、自聚集能力和群集泳动能力以及膜外聚合物的分泌来抑制其生物被膜形成,对探究益生菌代谢产物作为生物被膜抑制剂方面的应用具有重要的参考价值。 展开更多
关键词 枯草芽孢杆菌 无细胞上清液 单增李斯特菌 生物被膜 激光共聚焦显微镜
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培养间充质干细胞的上清液对食管癌细胞ECA109生物学行为的影响
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作者 许彭 张亚杰 +1 位作者 马莉莉 李秀梅 《现代肿瘤医学》 CAS 2024年第11期1967-1972,共6页
目的:探讨脐带间充质干细胞(mesenchymal stem cells,MSCs)培养的上清液对食管癌细胞ECA109增殖、迁移、凋亡等生物学行为的影响。方法:培养人脐带MSCs,提取过滤脐带MSCs培养的上清液与食管癌细胞ECA109共培养,通过细胞划痕实验和CCK-8... 目的:探讨脐带间充质干细胞(mesenchymal stem cells,MSCs)培养的上清液对食管癌细胞ECA109增殖、迁移、凋亡等生物学行为的影响。方法:培养人脐带MSCs,提取过滤脐带MSCs培养的上清液与食管癌细胞ECA109共培养,通过细胞划痕实验和CCK-8实验检测ECA109细胞迁移、增殖能力;采用流式细胞测定技术检测ECA109细胞凋亡情况;利用RT-PCR和Western blot检测相关通路中ERK、AKT、PI3K的表达水平。结果:划痕实验48 h,MSCs上清液共培养组ECA109细胞划痕面积为(17.157±1.425)μm^(2),明显高于对照组划痕面积(14.500±0.563)μm^(2)(P=0.04);CCK-8结果显示,48 h后MSCs上清液培养组ECA109细胞OD值(1.069±0.254)显著低于对照组(1.718±0.520)(P=0.037);流式细胞术检测结果显示MSCs上清液共培养组细胞早期凋亡率(12.36±2.47)%高于对照组(7.23±0.86)%;G2+S期MSCs上清液共培养组细胞比例(39.82±1.01)%相比对照组(55.17±9.60)%显著减少。RT-PCR结果显示,MSCs上清液共培养组AKT mRNA表达量(0.35±0.01)明显低于对照组(1.00±0.05)(P=0.004);Western blot可以看出,MSCs上清液共培养组ERK、AKT蛋白表达均低于对照组,差异有统计学意义。结论:MSCs上清液抑制食管癌细胞ECA109的增殖和迁移能力,促进ECA109细胞的凋亡,且ERK和AKT的蛋白质表达水平均下降。 展开更多
关键词 间充质干细胞上清液 食管癌 增殖迁移能力
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母体尿液铜蓝蛋白、子宫动脉搏动指数与妊娠前BMI联合用于子痫前期早期预测的价值研究
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作者 孙荣荣 顾颖 史玉霞 《中国性科学》 2024年第3期112-117,共6页
目的探讨候选蛋白联合子宫动脉多普勒超声和临床危险因素对子痫前期(PE)的预测价值。方法选取2021年1月1日至12月31日在无锡市妇幼保健院接受产前检查并分娩的60例PE孕妇作为研究对象,分为12例发现集和48例验证集。另外纳入同期同一医... 目的探讨候选蛋白联合子宫动脉多普勒超声和临床危险因素对子痫前期(PE)的预测价值。方法选取2021年1月1日至12月31日在无锡市妇幼保健院接受产前检查并分娩的60例PE孕妇作为研究对象,分为12例发现集和48例验证集。另外纳入同期同一医院60例无妊娠期并发症的正常孕妇作为对照。于孕11~13^(+6)周留存所有孕妇的尿液上清,使用超高效液相色谱-质谱法(UPLC-MS)和酶联免疫吸附试验法检测尿液上清蛋白;同时采用四维彩色多普勒超声记录子宫动脉搏动指数(UtA-PI);采用免疫组织化学染色法分析胎盘组织中蛋白表达。结果经UPLC-MS分析,在发现集中证实铜蓝蛋白在PE组上调,被作为候选蛋白进行验证。在验证集中,PE组孕妇在妊娠早期平均UtA-PI和尿液铜蓝蛋白均显著高于正常孕妇组(P<0.05)。尿液上清铜蓝白、妊娠前体重指数和异常UtA-PI可很好地预测在妊娠20周后出现PE的女性,灵敏度和特异度分别为0.813和0.937。此外,尿液上清铜蓝蛋白水平与胎盘组织铜蓝蛋白免疫组化平均光密度值呈正相关(r=0.624,P<0.001)。结论尿液铜蓝蛋白可能是PE的一种新的生物标志物,联合子宫动脉搏动指数和临床危险因素可能有助于PE的早期预测。 展开更多
关键词 子痫前期 尿液上清 蛋白组学 铜蓝蛋白 子宫动脉搏动指数
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基于非靶向代谢组学揭示灭活处理对植物乳植杆菌代谢物的影响
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作者 焦杨波 郑帅莉 赵洁 《食品与发酵工业》 CAS CSCD 北大核心 2024年第22期131-136,共6页
随着对益生菌的深入研究,研究人员发现某些灭活菌体及其代谢产物也展现出一定的应用潜力。该研究以植物乳植杆菌为研究对象,采用超高效液相色谱高分辨质谱非靶向代谢组学技术并结合正交偏最小二乘法判别分析多元统计分析方法,探究灭活... 随着对益生菌的深入研究,研究人员发现某些灭活菌体及其代谢产物也展现出一定的应用潜力。该研究以植物乳植杆菌为研究对象,采用超高效液相色谱高分辨质谱非靶向代谢组学技术并结合正交偏最小二乘法判别分析多元统计分析方法,探究灭活处理和未灭活处理植物乳植杆菌代谢物质的差异。结果表明,植物乳植杆菌灭活后共发现了24个潜在的差异代谢物(变量投影重要度>1,P<0.05,差异改变倍数>1.2或<0.8)。与不灭活处理相比,灭活处理后有19种上调的差异代谢物。KEGG富集分析表明,这些差异代谢物富集到的代谢通路有6条,差异极显著(P<0.01)的代谢通路是精氨酸和脯氨酸代谢,富集到这条代谢通路的差异代谢物有L-精氨酸和4-乙酰氨基丁酸酯。这些物质作为益生物质对于促进宿主健康、提高动物的生产性能、临床医疗领域的应用具有重要前景。该研究从代谢组学角度初步揭示了灭活前、后植物乳植杆菌代谢产物的差异性,为后续植物乳植杆菌的应用提供理论依据。 展开更多
关键词 植物乳植杆菌 代谢组学 灭活处理 差异代谢产物 离心上清液
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疏浚泥浆多级絮凝效能与絮体图像特征分析
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作者 程泽海 凌豪俊 +2 位作者 洪瑜泽 张超杰 杨关设 《浙江科技大学学报》 CAS 2024年第5期427-435,共9页
【目的】在水库疏浚工程中,亟须对产生的高含水率泥浆进行有效处理,故通过试验研究探索出一种泥浆多级絮凝的最佳方案,以实现泥浆快速高效脱水的目的。【方法】对有机高分子絮凝剂聚丙烯酰胺(polyacrylamide,PAM)、壳聚糖(chitosan,CTS... 【目的】在水库疏浚工程中,亟须对产生的高含水率泥浆进行有效处理,故通过试验研究探索出一种泥浆多级絮凝的最佳方案,以实现泥浆快速高效脱水的目的。【方法】对有机高分子絮凝剂聚丙烯酰胺(polyacrylamide,PAM)、壳聚糖(chitosan,CTS)单掺及其与明矾-聚氯化铝(alum and polyaluminum chloride,KAl(SO 4)2-PAC)无机絮凝剂组合进行对比研究,分析不同工况下PAM、CTS多级絮凝的去浊效果、形态特征与絮凝机理。【结果】PAM方案四级絮凝达到了最佳效果,此时上清液浊度降低至1.6浊度值(nephelometric turbidity unit,NTU),粒径大于1000μm的成像面积占比达95.82%,且分形维数达到最大值1.786,生成的絮体形态规则且呈团聚状,为最佳絮凝方案;多级絮凝机理分析结果表明,多级絮凝方式可以有效提升絮凝效能。【结论】本试验成果可为疏浚工程絮凝过程中实现自动控制提供理论依据。 展开更多
关键词 疏浚泥浆 多级絮凝 上清液浊度 分形维数 絮凝机理
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搅拌站废浆上清液及干粉在水泥中的作用机理
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作者 孙禹豪 何廷树 +2 位作者 潘小让 李娜 赵王卓 《新型建筑材料》 2024年第6期88-93,共6页
研究了搅拌站废浆上清液与干粉对水泥性能的影响机理及变化趋势。结果表明:废浆干粉会显著增加水泥用水量,当废浆干粉掺量为30%时,用水量增加29.2%。废浆上清液与干粉二者共用时,会略微降低水泥用水量。废浆干粉能够显著缩短水泥凝结时... 研究了搅拌站废浆上清液与干粉对水泥性能的影响机理及变化趋势。结果表明:废浆干粉会显著增加水泥用水量,当废浆干粉掺量为30%时,用水量增加29.2%。废浆上清液与干粉二者共用时,会略微降低水泥用水量。废浆干粉能够显著缩短水泥凝结时间,而上清液对水泥凝结有延缓作用,二者共用时,对凝结时间无明显影响。当干粉掺量不大于10%时,强度符合GB 175—2007《通用硅酸盐水泥》中P·O42.5R水泥的要求;上清液对水泥力学性能几乎没有影响,二者共用时,随着废浆上清液掺量的增加,水泥抗折、抗压强度降低。 展开更多
关键词 搅拌站废浆 干粉 上清液 力学性能
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聚醚废浓缩液絮凝上清液脱色工艺研究
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作者 李永峰 朱立新 +2 位作者 戚楠 马俊杰 孙体昌 《当代化工研究》 CAS 2024年第9期148-150,共3页
本文研究了双氧水氧化、活性炭吸附和二者组合对絮凝上清液脱色的影响。结果表明,双氧水氧化脱色率可以达到88.74%但脱色液随时间延长会有沉淀析出。凹凸棒土、白土、活性炭三种吸附剂脱色效果不理想,活性炭的脱色率可以达到30.69%,脱... 本文研究了双氧水氧化、活性炭吸附和二者组合对絮凝上清液脱色的影响。结果表明,双氧水氧化脱色率可以达到88.74%但脱色液随时间延长会有沉淀析出。凹凸棒土、白土、活性炭三种吸附剂脱色效果不理想,活性炭的脱色率可以达到30.69%,脱色上清液没有沉淀析出。双氧水氧化和活性炭吸附组合效果更好,脱色率可以达到94.52%且脱色上清液没有沉淀析出。脱色后的上清液可以作为水基切削液的原料使用。 展开更多
关键词 絮凝上清液 双氧水 氧化脱色 活性炭 吸附脱色
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Akkermansia muciniphila上清通过抑制内质网应激改善小肠黏膜损伤
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作者 张丽君 袁俊婕 +1 位作者 赵经文 陈鑫 《天津医科大学学报》 2024年第6期528-534,共7页
目的:探究Akkermansia muciniphila(AKK)上清对非甾体类抗炎药(NSAIDs)导致小肠黏膜损伤的干预效果及作用机制。方法:将18只C57BL/6小鼠(6~8周龄)采用随机数字表法随机分为3组:正常对照组(CON组)、造模组(INDO组)、干预组(INDO+AKK组),... 目的:探究Akkermansia muciniphila(AKK)上清对非甾体类抗炎药(NSAIDs)导致小肠黏膜损伤的干预效果及作用机制。方法:将18只C57BL/6小鼠(6~8周龄)采用随机数字表法随机分为3组:正常对照组(CON组)、造模组(INDO组)、干预组(INDO+AKK组),每组6只。INDO组使用吲哚美辛构建NSAIDs小肠损伤模型,INDO+AKK组使用AKK上清灌胃。采集各组肠道标本,常规苏木精-伊红(HE)染色观察黏膜屏障形态,免疫组织化学染色法(IHC)观察黏膜屏障指标咬合蛋白(Occuldin)、闭锁小带蛋白(ZO1)、黏蛋白2(MUC2)的表达水平。采用实时荧光定量PCR(qRT-PCR)从mRNA水平验证MUC2、葡萄糖调节蛋白78(GRP78)、X盒结合蛋白1(XBP1s)、C/EBP坏死因子相关蛋白(CHOP)、激活转录因子4(ATF4)、激活转录因子6(ATF6)、真核翻译起始因子2α激酶3(PERK)等基因的表达变化。结果:小肠组织HE染色观察发现,与CON组相比,INDO组出现小肠黏膜结构破坏、绒毛明显减少等特征。INDO+AKK组小肠黏膜损伤减轻、绒毛丧失现象减少、黏膜及其下层的炎症细胞浸润区域也显著减小。IHC分析显示,与INDO组相比,INDO+AKK组Occuldin(F=11.48,P<0.05)、ZO1(F=68.10,P<0.001)、MUC2(F=19.93,P<0.01)表达水平增加。PAS结果显示,INDO+AKK组杯状细胞数量相对于INDO组明显增加(F=205.9,P<0.001)。qRTPCR结果发现,与INDO组相比,INDO+AKK组MUC2的基因(F=23.67,P<0.01)表达水平升高,GRP78(F=7.869,P<0.01)、CHOP(F=11.45,P<0.05)、XBP1s(F=8.344,P<0.05)、ATF4(F=16.37,P<0.001)、ATF6(F=12.99,P<0.001)、PERK(F=11.58,P<0.01)的基因表达水平均下降。结论:AKK上清通过抑制内质网应激,改善NSAIDs诱导的小肠黏膜屏障损伤。 展开更多
关键词 AKK上清 内质网应激 NSAIDS 小肠黏膜损伤
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Impact of Paenibacillus elgii supernatant on screening bacterial strains with potential for biotechnological applications
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作者 I.C.Cunha-Ferreira C.S.Vizzotto +4 位作者 T.D.Frederico J.Peixoto L.S Carvalho M.R.Tótola R.H.Krüger 《Engineering Microbiology》 2024年第3期1-15,共15页
The biotechnological industry faces a crucial demand for novel bioactive compounds,particularly antimicrobial agents,to address the rising challenge of bacterial resistance to current available antibiotics.Traditional... The biotechnological industry faces a crucial demand for novel bioactive compounds,particularly antimicrobial agents,to address the rising challenge of bacterial resistance to current available antibiotics.Traditional strate-gies for cultivating naturally occurring microorganisms often limit the discovery of novel antimicrobial producers.This study presents a protocol for targeted selection of bacterial strains using the supernatant of Paenibacillus el-gii,which produces abundant signal molecules and antimicrobial peptides.Soil samples were inoculated in these enriched culture media to selectively cultivate bacteria resistant to the supernatant,indicating their potential to produce similar compounds.The bacterial strains isolated through this method were assessed for their antibac-terial activity.In addition,the functional annotation of the genome of one of these strains revealed several gene clusters of biotechnological interest.This study highlights the effectiveness of using this approach for selective cultivation of microorganisms with potential for biotechnological applications. 展开更多
关键词 PROSPECTING supernatant Antibacterial activity
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Cell-free supernatants of Bacillus inhibit the biofilms of Pseudomonas lundensis cultured with and without Acinetobacter johnsonii
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作者 WU Shiyuan LU Zhong +3 位作者 SHI Honghui ZHANG Jun LU Haixia ZHU Junli 《微生物学报》 CAS CSCD 北大核心 2024年第10期3886-3900,共15页
[Objective] Pseudomonas as one of the dominant spoilage bacteria highly form biofilms in chilled meat products and processing environment when contaminating single or mixed with other species.This study aims to invest... [Objective] Pseudomonas as one of the dominant spoilage bacteria highly form biofilms in chilled meat products and processing environment when contaminating single or mixed with other species.This study aims to investigate the antibiofilm properties of the cell-free supernatants(CFSs) of three Bacillus species isolated from fermented food and rice seeds on Pseudomonas lundensis(PL) or and Acinetobacter johnsonii(AJ) as mono-or dual-species.[Methods] Biofilm biomass,extracellular polymeric substances(EPSs),and biofilm structure were measured by crystal violet staining,spectrophotometry,confocal laser scanning microscopy(CLSM),respectively,as well as transcription of biofilm-related genes determined by qPCR.[Results] The CFSs of Bacillus amyloliquefaciens ZG08,B.velezensis B5,and B.subtilis YB11 inhibited the biofilm formation of PL and AJ without affecting their growth.The treatment with 50% CFSs of ZG08 and B5 decreased the cell viability of two biofilms by 12.73%–21.04%,which was higher than that of YB11(0.15%–4.38%).The inhibition rates of 50% CFSs of the three strains were 59.75%–79.59% against the PL biofilm and 63.62%–78.57% against the biofilm of PL+AJ,in which the CFS of YB11 had weaker activity.The content of exopolysaccharides and exoprotein in the two biofilms treated with these CFSs were reduced by 53.77%–73.30% and 54.84%–62.38%,respectively.The treatment with the three CFSs also reduced the adhesive cells,loosened biofilm structures,and thinned their thickness by 57.63%–74.49% and 60.43%–64.64%,respectively.Moreover,the CFSs of ZG08 and B5 effectively eradicated by 41.77%–69.79% against the mature biofilms of PL and PL+AJ,compared to weak activity of YB11.In addition,the antibiofilm activities of the three CFSs were stable under four enzyme digestion and heating conditions.Compared with the control,the CFSs of ZG08 and B5 significantly down-regulated the expression of six biofilm-related genes,lapA,alg44,pelG,luxR,wspR,and rpoS.[Conclusion] The CFSs of ZG08 and B5 have strong antibiofilm activities against PL and AJ as mono-or dual-species. 展开更多
关键词 Bacillus spp. cell-free supernatant ANTIBIOFILM Pseudomonas lundensis Acinetobacter johnsonii biofilm of mixed species
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