Decellularization method based on trypsin-digestion is widely used to construct small diameter vascular grafts.However,this method will reduce the opening angle of the blood vessel and result in the reduction of resid...Decellularization method based on trypsin-digestion is widely used to construct small diameter vascular grafts.However,this method will reduce the opening angle of the blood vessel and result in the reduction of residual stress.Residual stress reduced has an adverse effect on the compliance and permeability of small diameter vascular grafts.To improve the situation,acellular blood vessels were treated with glutaraldehyde and photooxidation crosslinking respectively,and the changes of opening angle,circumferential residual strain of native blood vessels,decellularized arteries and crosslinked blood vessels were measured by means of histological examination,scanning electron microscopy(SEM)and transmission electron microscopy(TEM)in this study.The opening angle of decellularized arteries significantly restored after photooxidation crosslinking(P紏0.0216),while that of glutaraldehyde crosslinking blood vessels reduced.The elastic fibers inside the blood vessels became densely rearranged after photooxidation crosslinking.The results of finite element simulation showed that the residual stress increased with the increase of opening angle.In this study,we found at the first time that photooxidation crosslinking method could significantly increase the residual stress of decellularized vessels,which provides biomechanical support for the development of new biomaterials of vascular grafts.展开更多
基金This work was supported by grants from the National Key R&D Program(2016YFC1101101,2016YFC1102305)Preface Technology Project of Chongqing(cstc2018jcyjAX0530,cstc2019jcyj-msxmX0329)+2 种基金Fundamental Research Funds for Central Universities(2018CDXYSW0023)the National Natural Science Foundation of China(30570453,31971242)We are also thankful for the support from the Chongqing Engineering Laboratory in Vascular Implants,the Public Experiment Center of State Bioindustrial Base(Chongqing),China.
文摘Decellularization method based on trypsin-digestion is widely used to construct small diameter vascular grafts.However,this method will reduce the opening angle of the blood vessel and result in the reduction of residual stress.Residual stress reduced has an adverse effect on the compliance and permeability of small diameter vascular grafts.To improve the situation,acellular blood vessels were treated with glutaraldehyde and photooxidation crosslinking respectively,and the changes of opening angle,circumferential residual strain of native blood vessels,decellularized arteries and crosslinked blood vessels were measured by means of histological examination,scanning electron microscopy(SEM)and transmission electron microscopy(TEM)in this study.The opening angle of decellularized arteries significantly restored after photooxidation crosslinking(P紏0.0216),while that of glutaraldehyde crosslinking blood vessels reduced.The elastic fibers inside the blood vessels became densely rearranged after photooxidation crosslinking.The results of finite element simulation showed that the residual stress increased with the increase of opening angle.In this study,we found at the first time that photooxidation crosslinking method could significantly increase the residual stress of decellularized vessels,which provides biomechanical support for the development of new biomaterials of vascular grafts.
