Interaction between tumour cells and macrophages enables cancer cells to evade immune detection and clearance by interfering with macrophage phagocytosis.The anti-phagocytic signals regulated by anti-phagocytic protei...Interaction between tumour cells and macrophages enables cancer cells to evade immune detection and clearance by interfering with macrophage phagocytosis.The anti-phagocytic signals regulated by anti-phagocytic proteins are termed"don't eat me"signals;these signals include sialic acidbinding immunoglobulin-type lectin-10(Siglec-10)and the recently revealed CD24 immune checkpoint(ICP).In this study,we demonstrate that targeting a specific glycan on CD24 exhibits the potential to inhibit ICP.Sambucus nigra agglutinin(SNA),a sialic acid-binding lectin,was employed to block CD24 and to enhance phagocytosis in melanoma tumours.In addition,we prepared SNA-conjugated hollow gold-iron oxide nanoparticles for photothermal therapy of tumours.Our findings show that the combination treatment of SNA-conjugated photothermal nanoparticles and near-infrared exposure successfully augments tumour cell phagocytosis both in vitro and in vivo models.展开更多
基金a National Research Foundation of Korea(NRF)grant the Korean government(MSIT)(Nos.2020R1A5A1018052,2017M3A7B8061942,2019R1A2C1006018,2021R1A4A5032463 and 2021M3H4A4079629,Republic of Korea)。
文摘Interaction between tumour cells and macrophages enables cancer cells to evade immune detection and clearance by interfering with macrophage phagocytosis.The anti-phagocytic signals regulated by anti-phagocytic proteins are termed"don't eat me"signals;these signals include sialic acidbinding immunoglobulin-type lectin-10(Siglec-10)and the recently revealed CD24 immune checkpoint(ICP).In this study,we demonstrate that targeting a specific glycan on CD24 exhibits the potential to inhibit ICP.Sambucus nigra agglutinin(SNA),a sialic acid-binding lectin,was employed to block CD24 and to enhance phagocytosis in melanoma tumours.In addition,we prepared SNA-conjugated hollow gold-iron oxide nanoparticles for photothermal therapy of tumours.Our findings show that the combination treatment of SNA-conjugated photothermal nanoparticles and near-infrared exposure successfully augments tumour cell phagocytosis both in vitro and in vivo models.
