Polydopamine-coated i-motif DNA/Gold nanoplatforms for synergistic photothermal-chemotherapy

The combination of photothermal therapywith chemotherapy has gradually developed into promising cancer therapy.Here,a synergistic photothermal-chemotherapy nanoplatform based on polydopamine(PDA)-coated gold nanoparticles(AuNPs)were facilely achieved via the in situ polymerization of dopamine(DA)on the surface of AuNPs.This nanoplatform exhibited augmented photothermal conversion efficiency and enhanced colloidal stability in comparison with uncoated PDA shell AuNPs.The i-motif DNA nanostructure was assembled on PDA-coated AuNPs,which could be transformed into a C-quadruplex structure under an acidic environment,showing a characteristic pH response.The PDA shell served as a linker between the AuNPs and the i-motif DNA nanostructure.To enhance the specific cellular uptake,the AS1411 aptamer was introduced to the DNA nanostructure employed as a targeting ligand.In addition,Dox-loaded NPs(DAu@PDA-AS141)showed the pH/photothermal-responsive release of Dox.The photothermal effect of DAu@PDA-AS141 elicited excellent photothermal performance and efficient cancer cell inhibition under 808 nm near-infrared(NIR)irradiation.Overall,these results demonstrate that the DAu@PDA-AS141 nanoplatform shows great potential in synergistic photothermal-chemotherapy.

Facile construction of targeted pH-responsive DNA-conjugated gold nanoparticles for synergistic photothermal-chemotherapy

Recently,stimuii-responsive DNA nano structure-based nanodevices have bee n applied for cancer therapy.In this study,pH-responsive i-motifDNA was modified on gold nanoparticles(AuNPs)via a facile,time-saving freeze-thaw method and utilized to construct stimuii-responsive drug nanocarriers.When the environment pH changes from 7.4 to 5.0,the i-motif DNA would be folded into four-stranded(C-quadruplex)that could be characterized by circular dichroism,and the characteristic of acid stimulate was verified by fluorescence resonanee energy transfer(FRET).To enhance specifical cellular uptake,MUC1 aptamer was employed as the targeting moiety.Doxorubicin(Dox)is an anticancer drug that can be efficiently intercalated into GC base pairs of DNA nanostructure to form drug-loaded nanovehicles(Dox@AuNP-MUCl).Additionally,owing to the excellent photothermal con version efficiency of AuNPs,the synergistic effect between chemotherapy and PTT can be readily achieved by 808 nm near-infrared(NIR)irradiation,which exhibits specifically and efficiently anticancer efficiency.Hence,this multifunctional drug carrier shows the potential for synergistic photothermal-chemotherapy.

Temperature‑Regulating Phase Change Fiber Scaffold Toward Mild Photothermal–Chemotherapy

Photothermal therapy(PTT)is a treatment that increases the temperature of tumors to 42–48℃,or even higher for tumor ablation.PTT has sparked a lot of attention due to its ability to induce apoptosis or increase sensitivity to chemotherapy.Excessive heat not only kills the tumor cells,but also damages the surrounding healthy tissue,reducing therapeutic accuracy and increasing the possible side effects.Herein,a phase change fiber(PCF)scaffold serving as a thermal trigger in mild photothermal–chemo tumor therapy is developed to regulate temperature and control drug release.These prepared PCFs,comprised of hollow carbon fibers(HCFs)loaded with lauric acid as a phase change material(PCM),can effectively store and release any excess heat generated by irradiating with a near-infrared(NIR)laser through the reversible solid–liquid transition process of the PCM.With this feature,the optimal PTT temperature of implanted PCF-based composite scaffolds was identified for tumor therapy with minimal normal tissue damage.In addition,controlled release of chemotherapeutic drugs and heat shock protein(HSP)inhibitors from the PCF-based composite scaffolds have been shown to improve the efficacy of mild PTT.The developed PCF-based scaffold sheds light on the development of a new generation of therapeutic scaffolds for thermal therapy.