Soybean mosaic virus （SMV）, a member of the genus Potyvirus, is a major pathogen of soybean plants in China, and 16 SMV strains have been identified nationwide based on a former detailed SMV classification system. A...Soybean mosaic virus （SMV）, a member of the genus Potyvirus, is a major pathogen of soybean plants in China, and 16 SMV strains have been identified nationwide based on a former detailed SMV classification system. As the P3 gene is thought to be involved in viral replication, systemic infection, pathogenicity, and overcoming resistance, knowledge of the P3 gene sequences of SMV and other potyviruses would be useful in efforts to know the genetic relationships among them and control the disease. P3 gene sequences were obtained from representative isolates of the above-mentioned 16 SMV strains and were compared with other SMV strains and 16 Potyvirus species from the National Center for Biotechnology GenBank database. The P3 genes from the 16 SMV isolates are composed of 1041 nucleotides, encoding 347 amino acids, and share 90.7-100% nucleotide （NT） sequence identities and 95.1-100% amino acid （AA） sequence identities. The P3 coding regions of the 16 SMV isolates share high identities （92.4-98.9% NT and 96.0-100% AA） with the reported Korean isolates, followed by the USA isolates （88.5-97.9% NT and 91.4-98.6% AA）, and share low identities （80.5-85.2% NT and 82.1-84.7% AA） with the reported HZ 1 and P isolates from Pinellia ternata. The sequence identities of the P3 genes between SMV and the 16 potyviruses varied from 44.4 to 81.9% in the NT sequences and from 21.4 to 85.3% in the AA sequences, respectively. Among them, SMV was closely related to Watermelon mosaic virus （WMV）, with 76.0-81.9% NT and 77.5-85.3% AA identities. In addition, the SMV isolates and potyvirus species were clustered into six distinct groups. All the SMV strains isolated from soybean were clustered in Group I, and the remaining species were clustered in other groups. A multiple sequence alignment analysis of the C-terminal regions indicated that the P3 genes within a species were highly conserved, whereas those among species were relatively variable.展开更多
快速准确的害虫种类识别是有效筛选天敌昆虫开展靶向性生物防控的关键。入侵种西花蓟马Frankliniella occidentalis与本地种花蓟马F.intonsa常同域同期同种寄主上发生,因体型小、形态相似,难以快速准确鉴定。本研究以mt DNA COI与r DNA ...快速准确的害虫种类识别是有效筛选天敌昆虫开展靶向性生物防控的关键。入侵种西花蓟马Frankliniella occidentalis与本地种花蓟马F.intonsa常同域同期同种寄主上发生,因体型小、形态相似,难以快速准确鉴定。本研究以mt DNA COI与r DNA ITS2基因为标记对我国10个采样点的西花蓟马和花蓟马开展分子鉴定研究。结果显示,当以COI或ITS2基因为靶标时,西花蓟马与花蓟马间的平均遗传距离分别为0.221和0.113,是种内遗传距离的22.1和18.8倍,且种内、种间遗传距离间无重叠,并在系统发育树上聚为独立的分支,表明2种基因均可准确区分西花蓟马和花蓟马。此外,基于COI基因构建的系统发育树,西花蓟马的各单倍型聚为了2个亚分支,并分别对应温室品系和羽扇豆品系,表明COI基因还可用于西花蓟马品系的识别鉴定。研究结果对近缘种花蓟马的快速鉴定、专食性天敌昆虫的筛选,及其生防效果评价具有重要意义。展开更多
基金supported by the National Natural Science Foundation of China(30671266,31101164)the National Basic Research Program of China(2006CB101708,2009CB118404)+2 种基金the National 863 Program of China(2006AA100104)the 111 Project from Ministry of Education of China(B08025)the Youth Science and Technology Innovation Foundation of Nanjing Agriculture University,China(KJ2010002)
文摘Soybean mosaic virus （SMV）, a member of the genus Potyvirus, is a major pathogen of soybean plants in China, and 16 SMV strains have been identified nationwide based on a former detailed SMV classification system. As the P3 gene is thought to be involved in viral replication, systemic infection, pathogenicity, and overcoming resistance, knowledge of the P3 gene sequences of SMV and other potyviruses would be useful in efforts to know the genetic relationships among them and control the disease. P3 gene sequences were obtained from representative isolates of the above-mentioned 16 SMV strains and were compared with other SMV strains and 16 Potyvirus species from the National Center for Biotechnology GenBank database. The P3 genes from the 16 SMV isolates are composed of 1041 nucleotides, encoding 347 amino acids, and share 90.7-100% nucleotide （NT） sequence identities and 95.1-100% amino acid （AA） sequence identities. The P3 coding regions of the 16 SMV isolates share high identities （92.4-98.9% NT and 96.0-100% AA） with the reported Korean isolates, followed by the USA isolates （88.5-97.9% NT and 91.4-98.6% AA）, and share low identities （80.5-85.2% NT and 82.1-84.7% AA） with the reported HZ 1 and P isolates from Pinellia ternata. The sequence identities of the P3 genes between SMV and the 16 potyviruses varied from 44.4 to 81.9% in the NT sequences and from 21.4 to 85.3% in the AA sequences, respectively. Among them, SMV was closely related to Watermelon mosaic virus （WMV）, with 76.0-81.9% NT and 77.5-85.3% AA identities. In addition, the SMV isolates and potyvirus species were clustered into six distinct groups. All the SMV strains isolated from soybean were clustered in Group I, and the remaining species were clustered in other groups. A multiple sequence alignment analysis of the C-terminal regions indicated that the P3 genes within a species were highly conserved, whereas those among species were relatively variable.
文摘快速准确的害虫种类识别是有效筛选天敌昆虫开展靶向性生物防控的关键。入侵种西花蓟马Frankliniella occidentalis与本地种花蓟马F.intonsa常同域同期同种寄主上发生,因体型小、形态相似,难以快速准确鉴定。本研究以mt DNA COI与r DNA ITS2基因为标记对我国10个采样点的西花蓟马和花蓟马开展分子鉴定研究。结果显示,当以COI或ITS2基因为靶标时,西花蓟马与花蓟马间的平均遗传距离分别为0.221和0.113,是种内遗传距离的22.1和18.8倍,且种内、种间遗传距离间无重叠,并在系统发育树上聚为独立的分支,表明2种基因均可准确区分西花蓟马和花蓟马。此外,基于COI基因构建的系统发育树,西花蓟马的各单倍型聚为了2个亚分支,并分别对应温室品系和羽扇豆品系,表明COI基因还可用于西花蓟马品系的识别鉴定。研究结果对近缘种花蓟马的快速鉴定、专食性天敌昆虫的筛选,及其生防效果评价具有重要意义。
