The prevalence of human papilloma virus (HPV)-16 in patients with cervical cancer,the physical status of HPV-16 in patients with cervical lesions,and the role of HPV-16 integration in cervi-cal carcinogenesis were inv...The prevalence of human papilloma virus (HPV)-16 in patients with cervical cancer,the physical status of HPV-16 in patients with cervical lesions,and the role of HPV-16 integration in cervi-cal carcinogenesis were investigated.HPV genotyping was performed by using PCR approach with the primer GP5+/GP6+ and type-specific primer on biopsy specimens taken operatively from 198 women.Multiple PCR was done to detect physical status of HPV-16 in a series of cervical liquid-based cytology samples and biopsy specimens obtained from different cervical lesions with HPV-16 infection,includ-ing 112 specimens with cervical cancer,151 specimens with CINⅠ,246 specimens with CINⅡ and 120 specimens with CINⅢ.The results showed that there were 112 cervical cancer samples (56.57% of total cervical cancer patients) with HPV-16 infection.The frequency of HPV-16 pure integration was 65.18% (73/112),56.57% (47/120),23.58% (58/246) and 7.95% (12/151) in cervical cancer,CINⅢ,CINⅡand CINⅠ patients respectively.In situ hybridization was performed on some paraffin-embedded sections of CINⅡ,CINⅢ and cervical cancer to verify the physical status of HPV-16 infection.Sig-nificant difference was observed between cervical cancer and CINⅠ,CINⅡ,CINⅢ in the frequency of HPV-16 integration (P<0.01).It is suggested that HPV-16 is the most prevalent type and is associated with cervical cancer.In the case of HPV-16 infection there are close associations between the severity of cervical lesions and the frequency of HPV-16 integration.The application of testing HPV genotyping and physical status based on detection of HC-Ⅱ HPV DNA would be in favor of predicting the progno-sis of cervical precancerosis and enhancing the screening accuracy of cervical cancer.展开更多
Written November 2013 by M. Carena （Fermi National Accelerator Laboratory and the University of Chicago）, C. Grojean （ICREA at IFAE, Universitat Autbnoma de Barcelona）, M, Kado （Laboratoire de l＇Accelerateur Lin...Written November 2013 by M. Carena （Fermi National Accelerator Laboratory and the University of Chicago）, C. Grojean （ICREA at IFAE, Universitat Autbnoma de Barcelona）, M, Kado （Laboratoire de l＇Accelerateur Lineaire, LAL and CERN）, and V. Sharma （University of California San Diego）.展开更多
基金supported by grants from National Natura Science Foundation of China (No. 81001151,No. 30973205)Natural Science Foundation of Hubei Province (No2010CDB09503)
文摘The prevalence of human papilloma virus (HPV)-16 in patients with cervical cancer,the physical status of HPV-16 in patients with cervical lesions,and the role of HPV-16 integration in cervi-cal carcinogenesis were investigated.HPV genotyping was performed by using PCR approach with the primer GP5+/GP6+ and type-specific primer on biopsy specimens taken operatively from 198 women.Multiple PCR was done to detect physical status of HPV-16 in a series of cervical liquid-based cytology samples and biopsy specimens obtained from different cervical lesions with HPV-16 infection,includ-ing 112 specimens with cervical cancer,151 specimens with CINⅠ,246 specimens with CINⅡ and 120 specimens with CINⅢ.The results showed that there were 112 cervical cancer samples (56.57% of total cervical cancer patients) with HPV-16 infection.The frequency of HPV-16 pure integration was 65.18% (73/112),56.57% (47/120),23.58% (58/246) and 7.95% (12/151) in cervical cancer,CINⅢ,CINⅡand CINⅠ patients respectively.In situ hybridization was performed on some paraffin-embedded sections of CINⅡ,CINⅢ and cervical cancer to verify the physical status of HPV-16 infection.Sig-nificant difference was observed between cervical cancer and CINⅠ,CINⅡ,CINⅢ in the frequency of HPV-16 integration (P<0.01).It is suggested that HPV-16 is the most prevalent type and is associated with cervical cancer.In the case of HPV-16 infection there are close associations between the severity of cervical lesions and the frequency of HPV-16 integration.The application of testing HPV genotyping and physical status based on detection of HC-Ⅱ HPV DNA would be in favor of predicting the progno-sis of cervical precancerosis and enhancing the screening accuracy of cervical cancer.
基金supported by Fermilab,that is operated by Fermi Research Alliance,LLC under Contract No.DE-AC02-07CH11359 with the United States Department of EnergyC.G.is supported by the Spanish Ministry MICINN under contract FPA2010-17747+2 种基金the European Commission under the ERC Advanced Grant 22637 MassTeVthe contract PITN-GA-2009-237920 UNILHC.M.K. is supported by the ANR HiggsNet grant.V.S.is supported by the grant DE-SC0009919 of the United States Department of Energy
文摘Written November 2013 by M. Carena （Fermi National Accelerator Laboratory and the University of Chicago）, C. Grojean （ICREA at IFAE, Universitat Autbnoma de Barcelona）, M, Kado （Laboratoire de l＇Accelerateur Lineaire, LAL and CERN）, and V. Sharma （University of California San Diego）.
