In May of 2007,the second generation selected (SS) and control (SC) groups of pearl oyster Pinctada martensii were established by selecting 10% breeders with the largest and mean shell length,respectively,from the...In May of 2007,the second generation selected (SS) and control (SC) groups of pearl oyster Pinctada martensii were established by selecting 10% breeders with the largest and mean shell length,respectively,from the first generation selected group.Growth performance of the SS and SC groups were compared on the basis of measurement data at Days 8,18,60,95,195 and 365.On Day 365,100 individuals (60.0–75.0 mm at shell length) were sampled from each group and then subjected to the experiment where physiological parameters such as filtrate rate,oxygen consumption and ammonia excretion were measured at 15,20,25 and 30°C.The results show that the SS group had significantly larger mean shell length and shell height than the SC group at Days 8,18,60,95,195 and 365 (P 0.05).The genetic gains at different ages varied from 6.0% to 17.0% for shell length and 5.7% to 14.6% for shell height,respectively.At 15,20,25 and 30 ° C,the SS groups had significantly larger filtrate rate than the SC group (P 0.05).At 15 and 25 °C,the differences in oxygen consumption rate between the SS and SC groups were not significant (P 0.05).At 20 and 30 °C,however,the oxygen consumption rate of the SS group was significantly larger than the SC group (P 0.05).At 15,20,25 and 30 °C,there were no significant differences in ammonia excretion rate between the SS and SC groups (P 0.05).The present results indicate that there existed considerable genetic variability in the base population and a further selection could be likely fruitful.Mass selection for faster growth might produce animals that had higher intake of metabolizable energy by virtue of faster filtrating behavior.展开更多
The experiment was set up for examining the physiological and biological indexes quickly and exactly, for obtaining information of tobacco-field fertilizing and tobacco growing. The ASD Field spec FR 2500 was used to ...The experiment was set up for examining the physiological and biological indexes quickly and exactly, for obtaining information of tobacco-field fertilizing and tobacco growing. The ASD Field spec FR 2500 was used to measure spectra reflectance of flue-cured tobacco and the relationship between hyperspectral parameters and biochemical contents (total nitrogen, chlorophyll, carotenoid), and physiological indexes (fresh weight, dry weight, moisture content) of flue-cured tobacco leaves was studied by correlation and stepwise regression statistic methods at different nitrogen and potassium levels. The results indicated that the spectra curves of different treatments had obvious rules and great diversities. There were high correlations between different types of spectra parameters and ten physiological and biochemical indexes of flue-cured tobacco leaves. Hyperspectral characteristic variables of ten physiological and biochemical indexes were found through stepwise regression, and SDr/SDb was the characteristic variable closest to seven biochemical contents. Simultaneously, the R2 and regression coefficient of equations reached 0.05 significant level and the equations had good estimating effects through the examination of other samples. Accordingly, this study suggested that the ten physiological and biochemical indexes could be estimated quickly by the estimating models, at the same time nitrogen-potassium fertilization and growth condition of flue-cured tobacco could be inspected.展开更多
The study was designated to explore the physiological mechanism of cold tolerance enhanced by phosphate in rice. An experiment was conducted to investigate the effects of different levels of phosphate fertilizer on co...The study was designated to explore the physiological mechanism of cold tolerance enhanced by phosphate in rice. An experiment was conducted to investigate the effects of different levels of phosphate fertilizer on cold tolerance and its related physiological parameters in rice seedings (chilling-sensitive cv. Changbai 9 and chilling-tolerant cv. Jijing 81) under low temperature stress. At the same time, the identification of cold tolerance was conducted. Compared with the normal temperature treatment, the relative chlorophyll content, photosynthesis rate, Fv/Fm and qP decreased and index of unsaturated fatty acid increased in rice under low temperature stress. The effect of chilling-sensitive cultivars was more than that of chilling-tolerant cultivars, more phosphorus fertilizer properly improved seedling quality of rice, slowed relative chlorophyll content dropping degree of rice seeding, increased photosynthesis rate, Fv/Fm, qP and index of unsaturated fatty acids, and enhanced the ability to chilling-tolerant cultivars under low temperature. The effect on chilling-tolerant cultivars was significantly higher than that on chilling sensitive cultivars by applying more phosphorus fertilizer. Phosphate regulated photosynthetic physiology and membrane fluidity to reduce injury by low temperature, and increasd the cold tolerance capacity of rice.展开更多
Winged bean (Psophocarpus tetragonolobus (L.) DC.) is a potential legume crop of the tropics with high protein and oil content in the seeds. Analysis of the mutual genotypic relationships among twenty four genotypes o...Winged bean (Psophocarpus tetragonolobus (L.) DC.) is a potential legume crop of the tropics with high protein and oil content in the seeds. Analysis of the mutual genotypic relationships among twenty four genotypes of P. tetragonolobus through Mantel test found a significant correlation (r = 0.839) between similarity matrices of the results obtained from the use of the RAPD and ISSR molecular markers. The UPGMA tree based on Jaccard’s similarity coefficient generated from their cumulative data showed two distinct clusters and seven sub-clusters among these accessions. Quantification of total polyphenols, flavonoids and tannin revealed the highest percentage of occurrence of kaempferol (1.07-790.5 μg/g) and the lowest percentage of gallic acid (0.09-3.49 μg/g) in the seeds. Phytochemical analysis of the winged bean genotypes revealed that, some of the exotic lines are distinct. Analysis of photosynthesis rate, photosynthetic yield and stomatal conductance data also showed two clusters and was in congruence with the phytochemical affinities of the genotypes. The overall high level of polymorphism and varied range of genetic distances across the genotypes revealed a wide range of genetic base of P. tetragonolobus. The present investigation therefore, has provided significant insights for further improvement of winged bean germplasm for its qualitative and quantitative traits.展开更多
Exposure to sound,heat,and increased physical workload can change physiological parameters.This study was conducted to evaluate the effect of concomitant exposure to sound,heat,and physical workload changes on physiol...Exposure to sound,heat,and increased physical workload can change physiological parameters.This study was conducted to evaluate the effect of concomitant exposure to sound,heat,and physical workload changes on physiological parameters in controlled laboratory conditions.This experimental crosssectional study was conducted in 35 male university students with a mean age of 25.75 years and a mean BMI of 22.69 kg/m2.Systolic and diastolic blood pressure and heart rate were measured after 15 min rest in the laboratory,5 and 10 min after starting the experiment,and then after 20 min in controlled laboratory conditions in five combination modes.The combination modes were(Sound:65 dB,WBGT:22°C,Speed:1.7,Slope:10%),(Sound:65 dB,WBGT:22°C,Speed:3.4,Slope:14%),(Sound:95 dB,WBGT:22°C,Speed:1.7,Slope:10%),(Sound:65 dB,WBGT:32°C,Speed:1.7,Slope:10%),and(Sound:95 dB,WBGT:32°C,Speed:3.4,Slope:14%).Mixed model analysis and paired t-test were applied for analysis.The results showed that the mean physiological parameters(Systolic and diastolic blood pressure and heart rate)increased when different combination modes worsened(Sound from 65 to 95 dB,WBGT from 22°C to 32°C,speed from 1.7 to 3.4,and slope from 10%to 14%,and when sound:95 dB,WBGT:32°C,Speed:3.4,and Slope:14%).Moreover,the mean changes of systolic and diastolic blood pressure were significant in all conditions when compared with the reference condition(Sound:65 dB,WBGT:22°C,Speed:1.7,and Slope:10%).The mean heart rate changes were also significant except for exposure to the second condition(Sound:65 dB,WBGT:22°C,Speed:3.4,Slope:14%)and the third condition(Sound:95 dB,WBGT:22°C,Speed:1.7,Slope:10%).Exposure to hazardous levels of sound,heat,and workload has adverse effects on physiological parameters.Concomitant exposure to all three hazards has a synergistic effect and increases the adverse effect.展开更多
Background: Anesthetic agents are commonly utilized in the handling of non‐human primates for prevent the stress caused in physical exploration or physical restrain. For this reason, the objective of this work was to...Background: Anesthetic agents are commonly utilized in the handling of non‐human primates for prevent the stress caused in physical exploration or physical restrain. For this reason, the objective of this work was to describe the effect of age and dissociative anesthetics (ketamine and tiletamine), and their combinations with acepromazine, xylazine and zolazepam, on the physiological and blood biochemical parameters in Macaca mulatta. Methods: Eighty male Macaca mulatta were divided into four experimental groups depending on the anesthetic mixture applied. Each group of 20 males was divided into five sub‐groups according to age. Physiological parameters were recorded every five minutes during a 30‐minute period. A blood sample was drawn to analyze blood biochemistry. Results: Statistical analyses revealed significant differences in the physiological parameters between the ketamine‐acepromazine and ketamine‐xylazine groups compared to the control group. The analysis of blood biochemistry found significant differences by age and by anesthetic mixture among all groups. Conclusion: These findings contribute to standardizing this animal model in biological research.展开更多
[ Objective] This study aimed to investigate the effects of 1-naphthol on primary productivity in aquatic ecosystems. [ Method] The effects of 1-naphthol on biomass, chlorophyll a content, malondialdehyde (MDA) cont...[ Objective] This study aimed to investigate the effects of 1-naphthol on primary productivity in aquatic ecosystems. [ Method] The effects of 1-naphthol on biomass, chlorophyll a content, malondialdehyde (MDA) content and soluble protein content of Chlorella vulgaris were investigated. [ Result] 1-Naphthol could significantly inhibit the growth of Chlorella vulgaris. On the 7^th d, the biomass of Chlorella vulgaris exposed to 1.0, 3.0, 6.0, 12.0 and 18.0 mg/L 1-naphthol was 60.26%, 48.15%, 41.32%, 28.59% and 27.10% of that in control respectively, exhibiting a significant dose-response relationship. Chlorella vulgaris was exposed to different concentrations of 1-naphthol (0, 1,5 and 10 mg/L) for 7 d ; with the increase of 1-naphthol concentration, chlorophyll a content of Ch/orella vulgaris was reduced, MDA content increased gradually, soluble protein content increased first and then declined. [ Conclusion] According to the experimental results, 1-naphthol can significantly inhibit the growth of Chlorella vulgaris, thus reducing primary productivity in aquatic ecosystems.展开更多
The study was conducted to investigate the characteristics of blood physiological parameters of Apodemus agrarius (A. agrarius). Blood physiological pa- rammers of 10 A. agrarius (half male and half female) were d...The study was conducted to investigate the characteristics of blood physiological parameters of Apodemus agrarius (A. agrarius). Blood physiological pa- rammers of 10 A. agrarius (half male and half female) were determined by automated hematology analyzer. Results showed that difference of A. agrarius hematokrit (HCT) between male and female was significant (P 〈 0.05 ), and differences of all the other blood physiological parameters between male and female were not sig- nificant ( P 〉 0.05 ). In the study, blood physiological reference value of A. agrarius was set up, which provided a basis for the bio-research and prophylaxis and treatment of A. agrarius.展开更多
Drought and salinity are the most widespread soil problems, posing a big threat to food security in rice growing regions. The present study evaluated the performance of eleven rice genotypes using morphological and ph...Drought and salinity are the most widespread soil problems, posing a big threat to food security in rice growing regions. The present study evaluated the performance of eleven rice genotypes using morphological and physiological parameters, under induced drought and salinity conditions. The seedlings were raised in 5 kg of homogenous soil in plastic bags in the </span><span style="font-family:Verdana;">greenhouse</span><span style="font-family:""><span style="font-family:Verdana;">. For the drought experiment, each bag was watered with 200 ml of water twice daily until plants reached the five-leaf stage when watering was suspended for 2 weeks for the drought stressed plants but not suspended for the control plants. The experiment was a 2 × 11 factorial and the set up was arranged using the completely randomized design with three replications. Data were taken on Plant height, Number of tillers, leaf length, Number of green leaves, Number of dead leaves, Leaf rolling score (LRS) and Rate of water loss. The salinity experiment was set up in a similar manner except that the plants were irrigated twice a day for 2 weeks with 200 ml of treatment solution containing either 0 mM NaCl or 75 mM and data were collected on plant height, number of tillers, shoot fresh weight, shoot dry weight, Na</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;"> and K</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;"> concentrations, relative water content and chlorophyll content. Data from both experiments were subjected to Analysis of variance test using the GenStat software 10</span><sup><span style="font-family:Verdana;">th</span></sup><span style="font-family:Verdana;"> edition and the means separated using least significant difference test. Individual stress response index (ISRI) was calculated for each parameter and the means used in grouping the varieties. Of the genotypes evaluated, four (FARO 44, NERICA 2, NERICA 8 and NERICA 5) were identified as tolerant, two (NERICA 4 and FARO 57) as moderately tolerant, while the rest were found to be sensitive to drought. Equally, two varieties (FARO 44 and RAM 137) stood out in the salinity screening as tolerant varieties, five were moderately tolerant while four (FARO 64, FARO 52, NERICA 2 and FARO 55) were clearly susceptible. FARO 44 is the only genotype that </span><span style="font-family:Verdana;">showed </span></span><span style="font-family:Verdana;">tolerance</span><span style="font-family:""> </span><span style="font-family:Verdana;">to both drought and salinity. The identified drought and salinity tolerant rice genotypes from this study can be recommended as genetic sources for future breeding programs for drought and salinity </span><span style="font-family:Verdana;">tolerance</span><span style="font-family:""> </span><span style="font-family:Verdana;">in rice.展开更多
This study investigated the effect of fixed height standing-workstation on different people with diverse anthropometry dimensions. Measurements of some anthropometric and physiological parameters are carried out as ba...This study investigated the effect of fixed height standing-workstation on different people with diverse anthropometry dimensions. Measurements of some anthropometric and physiological parameters are carried out as bases for the calculation of body mass index (BMI) and the determination of the maximum heart rate (HRmax) and aerobic power (VO2max) of individual subject while performing manual cutting operation with hacksaw on fixed vice height of 940 mm. Twenty subjects (S1 to S20) parted 2 mm thick square-pipe of 25 mm × 25 mm. Each subject carried out cutting operation in 5 replicates and their physiological parameters during activities are measured to determine their expended energy (EE) and oxygen consumption rate (VO2). The results showed that subject S4 with BMI of 20.76 kg/m2 has maximum cutting rate of 5.33 stroke/s, while subject S8 with BMI of 23.39 kg/m2 has minimum cutting rate of 0.92 stroke/s. There was a statistically significant effect on the interaction between BMI, EE and Cutting rate, with F = 827.54, P = 0.000, R2 = 0.967 and S = 1.749 units. Subject S11 was discovered to have VO2 (28.54 l/min) and VO2max (24.36 ml/min/kg), with highest value of EE (2.94 kcal/min). Wear rates of 1.86 teeth/s and 9.55 teeth/s have the same energy cost (EE = 0.87 kcal/min) but different cutting time of 36.65 s (S18) and 10.89 s (S20) respectively. This could explain in-part that excess 25.76 s utilized in operation time by subject S18 is responsible for keeping approximately 7.7 teeth intact as regards tool management. EE and Tool Wear Rate in one-way analysis of variance, were statistically significant (F = 45.87, P = 0.000, R2 = 54.69% and S = 1.617 units) at 0.05 level.展开更多
Rose (Rosa hybrida L.) is one of the most important specialty cut flowers produced. Characterization of physiological variability in photosynthetic efficiencies, respiration rate and chlorophyll content is one of the ...Rose (Rosa hybrida L.) is one of the most important specialty cut flowers produced. Characterization of physiological variability in photosynthetic efficiencies, respiration rate and chlorophyll content is one of the greatest challenges in assessing rose net primary production of flowering, quality of cut flowers and other rose uses. Two modern rose varieties Floribunda and Hybrid Tea were used to optimize different methods for analysing physiological characteristics (photosynthesis rate, respiration rate and chlorophyll content). Many parameters were optimized, five different lights intensity were used (600, 900, 1200, 1500 and 2000 μmol·m-2·s-1) of which 1200 μmol·m-2·s-1 gave the highest photosynthesis rate. Five different measuring times were used (8 am, 10 am, 12 pm, 2 pm and 4 pm) and 12 pm was shown to be the optimum time for measuring photosynthesis rate. Among two different weather conditions (sunny day and cloudy day) sunny day was selected. For respiration rate two different measuring times (1 and 2 hours) after darkness were studied and 1 hour was chosen. Among three different times (10 am, 12 pm and 2 pm) for measuring chlorophyll content, 12 pm was selected. Using these optimized variables will allow researchers to collect robust and reproducible results to be obtained from different studies, and in turn lead to improved yields of horticultural plants.展开更多
Exploring the response differences of leaf physiology parameters to enhanced nitrogen deposition between saplings and trees is vital for predicting the variations of terrestrial ecosystem structure and function under ...Exploring the response differences of leaf physiology parameters to enhanced nitrogen deposition between saplings and trees is vital for predicting the variations of terrestrial ecosystem structure and function under future global climate change. In this study, the ecophysiological parameters of saplings and trees of Fraxinus mandshurica Rupr. were measured at different levels of nitrogen addition in a temperate forest. The results show that ecophysiological parameters maximum net photosynthetic rate(Pmax), apparent quantum efficiency(a), dark respiration(Rd), light saturation point(Lsp), photosynthetic nitrogen use efficiency(PNUE),specific leaf area(SLA)and stomatal conductance under saturated light intensity(Gsmax) were higher in saplings than in trees. These physiological parameters and not Nleaf(leaf nitrogen content)led to relatively lower Pmaxand Rdin trees. For both saplings and trees, low and median nitrogen addition(23 and 46 kg ha-1a-1) resulted in significant increases in Pmax, Rd, Lsp, Chl, PNUE, SLA and Gsmax. These parameters tended to decline under high additions of nitrogen(69 kg ha-1a-1),whereas Nleaf was always enhanced with increasing nitrogen. Variations in Pmax and Rd with increasing nitrogen were attributed to variations in the strongly related parameters of, Lsp, Chl, PNUE, SLA and Gsmax. Overall, the response sensitivity of physiological parameters to enhanced nitrogen levels was lower in trees compared with saplings.展开更多
Objective:To analyse the seasonal effect on physiological parameters, reproductive profiles andin vitro fertility in breeding mithun bulls.Methods: A total of ten adult mithun bulls age of 5 to 6 years old with good b...Objective:To analyse the seasonal effect on physiological parameters, reproductive profiles andin vitro fertility in breeding mithun bulls.Methods: A total of ten adult mithun bulls age of 5 to 6 years old with good body condition (score 5-6) were selected from ICAR-NRC on Mithun, Jharnapani, Nagaland, India. The seasons were categorised into winter, spring, summer and autumn seasons based on the meteorological data and sunshine hours. The physiological parameters, reproductive profiles andin vitro fertility parameters were assessed during different seasons in mithun under the semi-intensive system of management.Results:The statistical analysis revealed that these experimental parameters were differed significantly (P<0.05) among the seasons and in overall spring and winter seasons were more beneficial in mithun breeding programme, although, the breeding in mithun occurred throughout the year with variation.Conclusions:It is concluded that collection & preservation of mithun semen and artificial insemination in mithun species during the season of spring and winter has significant beneficial effect in terms of semen production, freezability and fertility for artificial breeding programme in mithun under the semi-intensive system.展开更多
Understanding the mechanisms of drought resistance in crop species is crucial for the selection and breeding of tolerant rapeseed(Brassica napus L.)varieties.The present study aimed to assess the physiological and ana...Understanding the mechanisms of drought resistance in crop species is crucial for the selection and breeding of tolerant rapeseed(Brassica napus L.)varieties.The present study aimed to assess the physiological and anatomical responses of two rapeseed genotypes,P287(drought-tolerant)and T88(drought-sensitive)under three intensities of drought stress.All physiological and anatomical parameters related to drought acclimation were significantly altered in both genotypes under stress conditions.At the fourth-leaf stage,the relative water content,chlorophyll content,protein content,malondialdehyde content,and the activities of peroxidase and catalase in P287 were significantly higher than those in T88,particularly under severe drought conditions.After rehydration,all physiological indexes recovered rapidly,especially in P287.In addition,under drought stress,compared with T88,P287 had thicker palisade tissue,thinner spongy tissue,higher ratio of chloroplast length to chloroplast width,higher stomatal density and stomatal closure rate.Overall,the interaction between physiological and anatomical features improved the drought tolerance of P287 under drought stress conditions.展开更多
Split-root solution culture was used to study the promoting effect of lanthanum on rice (Oryza sativa) growth and its physiological mechanisms. Results sho w that low concentration (0.05~1.5 mg·L -1) increases...Split-root solution culture was used to study the promoting effect of lanthanum on rice (Oryza sativa) growth and its physiological mechanisms. Results sho w that low concentration (0.05~1.5 mg·L -1) increases rice yield an d grain numbers. High concentration depresses grain formation (9~30 mg·L -1 ) and root elongation (1.5~30 mg·L -1). No significant influence on str aw dry weight was found over the whole concentration range except the 0.05 mg·L -1 treatment. With the increase of La concentration from 0.05 to 0.75 mg· L -1, catalase (CAT) activity in the first fully expandeing leaves and root s decreases. When La concentration is greater than 0.75 mg·L -1 or less than 9 mg·L -1, it significantly decreases superoxide dismutase activity ( SOD) in the leaves and roots. No significant effects were found on chlorophyll, protein and malondialdehyde (MDA) content. Possible mechanisms of La′s promotin g effect on rice growth and reduction effect of ·O- 2 were discussed.展开更多
Heavy metal pollution has become a worldwide problem in aquaculture. We studied copper (Cu2+ ) accumulation and physiological responses of two red algae Gracilaria lemaneiformis and Gracilaria lichenoides from China u...Heavy metal pollution has become a worldwide problem in aquaculture. We studied copper (Cu2+ ) accumulation and physiological responses of two red algae Gracilaria lemaneiformis and Gracilaria lichenoides from China under Cu2+ exposure of 0-500 μg/L in concentration. Compared with G. lemaneiformis, G. lichenoides was more capable in accumulating Cu2+ , specifically, more Cu2+ on extracellular side (cell wall) than on intracellular side (cytoplasm) and in cell organelles (especially chloroplast, cell nucleus, mitochondria, and ribosome). In addition, G. lichenoides contained more insoluble polysaccharide in cell wall, which might promote the extracellular Cu2+ -binding as an efficient barrier against metal toxicity. Conversely, G. lemaneiformis was more vulnerable than G. lichenoides to Cu 2+ toxin for decreases in growth, pigment (chlorophyll a, chlorophyll b, phycobiliprotein, and β-carotene) content, and photosynthetic activity. Moreover, more serious oxidative damages in G. lemaneiformis than in G. lichenoides, in accumulation of reactive oxidative species and malondialdehyde, and in electrolyte leakage, because of lower antioxidant enzyme (superoxide dismutase and glutathione reductase) activities. Therefore, G. lichenoides was less susceptible to Cu2+ stress than G. lemaneiformis.展开更多
The majority of rice(Oryza sativa L.) produced in the southern USA is drill-seeded and grown under upland-like conditions because permanent flooding is established after the four-leaf stage. Therefore, rice during the...The majority of rice(Oryza sativa L.) produced in the southern USA is drill-seeded and grown under upland-like conditions because permanent flooding is established after the four-leaf stage. Therefore, rice during the seedling growth stage will be subjected to variable soil moisture content. A greenhouse experiment was conducted to evaluate the performance of 15 rice cultivars commonly grown in Mississippi of USA under early-season soil moisture stress. Twenty morpho-physiological parameters of rice seedlings subjected to three different levels(100%, 66% and 33% field capacity) of soil moisture, from 10 to 30 d after sowing, were measured. Significant moisture stress × treatment interaction(P < 0.001) was observed for most of the parameters. Further, the total drought response index(TDRI) was developed to score the cultivars for drought tolerance with the variation from 26.88 to 36.21. Accordingly, the cultivars were classified into different groups of tolerance. The cultivars CL152 and CL142-AR were classified as the least and the most tolerant to drought based on TDRI and standard deviation, respectively. Even though both total root(R^2 = 0.98) or shoot(R^2 = 0.76) drought responses indices were positively correlated with TDRI, root traits were important in deriving the indices. Therefore, TDRI could be used to select cultivars for drought tolerance in a given environment and develop rice varieties with early-season drought tolerance. However, further research is needed to identify and characterize tolerance at other stages to assist breeding programs in rice.展开更多
基金The National Key Technology R&D Program in the 11th Five Year Plan of China under contract No. 2007BAD29B01-2National Department Public Benefit Research Foundation under contract No. nyhyzx 07-048Guangdong Marine and Fishery Bureau under contract Nos A200708C01, A200908A02 and A200908A05
文摘In May of 2007,the second generation selected (SS) and control (SC) groups of pearl oyster Pinctada martensii were established by selecting 10% breeders with the largest and mean shell length,respectively,from the first generation selected group.Growth performance of the SS and SC groups were compared on the basis of measurement data at Days 8,18,60,95,195 and 365.On Day 365,100 individuals (60.0–75.0 mm at shell length) were sampled from each group and then subjected to the experiment where physiological parameters such as filtrate rate,oxygen consumption and ammonia excretion were measured at 15,20,25 and 30°C.The results show that the SS group had significantly larger mean shell length and shell height than the SC group at Days 8,18,60,95,195 and 365 (P 0.05).The genetic gains at different ages varied from 6.0% to 17.0% for shell length and 5.7% to 14.6% for shell height,respectively.At 15,20,25 and 30 ° C,the SS groups had significantly larger filtrate rate than the SC group (P 0.05).At 15 and 25 °C,the differences in oxygen consumption rate between the SS and SC groups were not significant (P 0.05).At 20 and 30 °C,however,the oxygen consumption rate of the SS group was significantly larger than the SC group (P 0.05).At 15,20,25 and 30 °C,there were no significant differences in ammonia excretion rate between the SS and SC groups (P 0.05).The present results indicate that there existed considerable genetic variability in the base population and a further selection could be likely fruitful.Mass selection for faster growth might produce animals that had higher intake of metabolizable energy by virtue of faster filtrating behavior.
文摘The experiment was set up for examining the physiological and biological indexes quickly and exactly, for obtaining information of tobacco-field fertilizing and tobacco growing. The ASD Field spec FR 2500 was used to measure spectra reflectance of flue-cured tobacco and the relationship between hyperspectral parameters and biochemical contents (total nitrogen, chlorophyll, carotenoid), and physiological indexes (fresh weight, dry weight, moisture content) of flue-cured tobacco leaves was studied by correlation and stepwise regression statistic methods at different nitrogen and potassium levels. The results indicated that the spectra curves of different treatments had obvious rules and great diversities. There were high correlations between different types of spectra parameters and ten physiological and biochemical indexes of flue-cured tobacco leaves. Hyperspectral characteristic variables of ten physiological and biochemical indexes were found through stepwise regression, and SDr/SDb was the characteristic variable closest to seven biochemical contents. Simultaneously, the R2 and regression coefficient of equations reached 0.05 significant level and the equations had good estimating effects through the examination of other samples. Accordingly, this study suggested that the ten physiological and biochemical indexes could be estimated quickly by the estimating models, at the same time nitrogen-potassium fertilization and growth condition of flue-cured tobacco could be inspected.
基金Supported by the Special Agricultural Project of Agricultural Department (200903003)the Agricultural Modernization Project in the Science and Technology Development Plan of Jilin Province (2009-2010) the High-yield Project of Science and Technology Department (2011BAD16B10)
文摘The study was designated to explore the physiological mechanism of cold tolerance enhanced by phosphate in rice. An experiment was conducted to investigate the effects of different levels of phosphate fertilizer on cold tolerance and its related physiological parameters in rice seedings (chilling-sensitive cv. Changbai 9 and chilling-tolerant cv. Jijing 81) under low temperature stress. At the same time, the identification of cold tolerance was conducted. Compared with the normal temperature treatment, the relative chlorophyll content, photosynthesis rate, Fv/Fm and qP decreased and index of unsaturated fatty acid increased in rice under low temperature stress. The effect of chilling-sensitive cultivars was more than that of chilling-tolerant cultivars, more phosphorus fertilizer properly improved seedling quality of rice, slowed relative chlorophyll content dropping degree of rice seeding, increased photosynthesis rate, Fv/Fm, qP and index of unsaturated fatty acids, and enhanced the ability to chilling-tolerant cultivars under low temperature. The effect on chilling-tolerant cultivars was significantly higher than that on chilling sensitive cultivars by applying more phosphorus fertilizer. Phosphate regulated photosynthetic physiology and membrane fluidity to reduce injury by low temperature, and increasd the cold tolerance capacity of rice.
文摘Winged bean (Psophocarpus tetragonolobus (L.) DC.) is a potential legume crop of the tropics with high protein and oil content in the seeds. Analysis of the mutual genotypic relationships among twenty four genotypes of P. tetragonolobus through Mantel test found a significant correlation (r = 0.839) between similarity matrices of the results obtained from the use of the RAPD and ISSR molecular markers. The UPGMA tree based on Jaccard’s similarity coefficient generated from their cumulative data showed two distinct clusters and seven sub-clusters among these accessions. Quantification of total polyphenols, flavonoids and tannin revealed the highest percentage of occurrence of kaempferol (1.07-790.5 μg/g) and the lowest percentage of gallic acid (0.09-3.49 μg/g) in the seeds. Phytochemical analysis of the winged bean genotypes revealed that, some of the exotic lines are distinct. Analysis of photosynthesis rate, photosynthetic yield and stomatal conductance data also showed two clusters and was in congruence with the phytochemical affinities of the genotypes. The overall high level of polymorphism and varied range of genetic distances across the genotypes revealed a wide range of genetic base of P. tetragonolobus. The present investigation therefore, has provided significant insights for further improvement of winged bean germplasm for its qualitative and quantitative traits.
基金The authors sincerely thank the deputy of research and technology affiliated to Shiraz University of Medical Sciences for financial support from this study,in the form of a research project by Mr.Hossein Molaeifar approved by the University on No.10652.
文摘Exposure to sound,heat,and increased physical workload can change physiological parameters.This study was conducted to evaluate the effect of concomitant exposure to sound,heat,and physical workload changes on physiological parameters in controlled laboratory conditions.This experimental crosssectional study was conducted in 35 male university students with a mean age of 25.75 years and a mean BMI of 22.69 kg/m2.Systolic and diastolic blood pressure and heart rate were measured after 15 min rest in the laboratory,5 and 10 min after starting the experiment,and then after 20 min in controlled laboratory conditions in five combination modes.The combination modes were(Sound:65 dB,WBGT:22°C,Speed:1.7,Slope:10%),(Sound:65 dB,WBGT:22°C,Speed:3.4,Slope:14%),(Sound:95 dB,WBGT:22°C,Speed:1.7,Slope:10%),(Sound:65 dB,WBGT:32°C,Speed:1.7,Slope:10%),and(Sound:95 dB,WBGT:32°C,Speed:3.4,Slope:14%).Mixed model analysis and paired t-test were applied for analysis.The results showed that the mean physiological parameters(Systolic and diastolic blood pressure and heart rate)increased when different combination modes worsened(Sound from 65 to 95 dB,WBGT from 22°C to 32°C,speed from 1.7 to 3.4,and slope from 10%to 14%,and when sound:95 dB,WBGT:32°C,Speed:3.4,and Slope:14%).Moreover,the mean changes of systolic and diastolic blood pressure were significant in all conditions when compared with the reference condition(Sound:65 dB,WBGT:22°C,Speed:1.7,and Slope:10%).The mean heart rate changes were also significant except for exposure to the second condition(Sound:65 dB,WBGT:22°C,Speed:3.4,Slope:14%)and the third condition(Sound:95 dB,WBGT:22°C,Speed:1.7,Slope:10%).Exposure to hazardous levels of sound,heat,and workload has adverse effects on physiological parameters.Concomitant exposure to all three hazards has a synergistic effect and increases the adverse effect.
文摘Background: Anesthetic agents are commonly utilized in the handling of non‐human primates for prevent the stress caused in physical exploration or physical restrain. For this reason, the objective of this work was to describe the effect of age and dissociative anesthetics (ketamine and tiletamine), and their combinations with acepromazine, xylazine and zolazepam, on the physiological and blood biochemical parameters in Macaca mulatta. Methods: Eighty male Macaca mulatta were divided into four experimental groups depending on the anesthetic mixture applied. Each group of 20 males was divided into five sub‐groups according to age. Physiological parameters were recorded every five minutes during a 30‐minute period. A blood sample was drawn to analyze blood biochemistry. Results: Statistical analyses revealed significant differences in the physiological parameters between the ketamine‐acepromazine and ketamine‐xylazine groups compared to the control group. The analysis of blood biochemistry found significant differences by age and by anesthetic mixture among all groups. Conclusion: These findings contribute to standardizing this animal model in biological research.
基金Supported by Special Fund for the Construction of Modern Agricultural IndustryTechnology System(CARS-49)
文摘[ Objective] This study aimed to investigate the effects of 1-naphthol on primary productivity in aquatic ecosystems. [ Method] The effects of 1-naphthol on biomass, chlorophyll a content, malondialdehyde (MDA) content and soluble protein content of Chlorella vulgaris were investigated. [ Result] 1-Naphthol could significantly inhibit the growth of Chlorella vulgaris. On the 7^th d, the biomass of Chlorella vulgaris exposed to 1.0, 3.0, 6.0, 12.0 and 18.0 mg/L 1-naphthol was 60.26%, 48.15%, 41.32%, 28.59% and 27.10% of that in control respectively, exhibiting a significant dose-response relationship. Chlorella vulgaris was exposed to different concentrations of 1-naphthol (0, 1,5 and 10 mg/L) for 7 d ; with the increase of 1-naphthol concentration, chlorophyll a content of Ch/orella vulgaris was reduced, MDA content increased gradually, soluble protein content increased first and then declined. [ Conclusion] According to the experimental results, 1-naphthol can significantly inhibit the growth of Chlorella vulgaris, thus reducing primary productivity in aquatic ecosystems.
基金Supported by Science and Technology Innovation Team Project of Universities in Heilongjiang Province&Graduate Academic Innovation Project of Mudanjiang Normal University
文摘The study was conducted to investigate the characteristics of blood physiological parameters of Apodemus agrarius (A. agrarius). Blood physiological pa- rammers of 10 A. agrarius (half male and half female) were determined by automated hematology analyzer. Results showed that difference of A. agrarius hematokrit (HCT) between male and female was significant (P 〈 0.05 ), and differences of all the other blood physiological parameters between male and female were not sig- nificant ( P 〉 0.05 ). In the study, blood physiological reference value of A. agrarius was set up, which provided a basis for the bio-research and prophylaxis and treatment of A. agrarius.
文摘Drought and salinity are the most widespread soil problems, posing a big threat to food security in rice growing regions. The present study evaluated the performance of eleven rice genotypes using morphological and physiological parameters, under induced drought and salinity conditions. The seedlings were raised in 5 kg of homogenous soil in plastic bags in the </span><span style="font-family:Verdana;">greenhouse</span><span style="font-family:""><span style="font-family:Verdana;">. For the drought experiment, each bag was watered with 200 ml of water twice daily until plants reached the five-leaf stage when watering was suspended for 2 weeks for the drought stressed plants but not suspended for the control plants. The experiment was a 2 × 11 factorial and the set up was arranged using the completely randomized design with three replications. Data were taken on Plant height, Number of tillers, leaf length, Number of green leaves, Number of dead leaves, Leaf rolling score (LRS) and Rate of water loss. The salinity experiment was set up in a similar manner except that the plants were irrigated twice a day for 2 weeks with 200 ml of treatment solution containing either 0 mM NaCl or 75 mM and data were collected on plant height, number of tillers, shoot fresh weight, shoot dry weight, Na</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;"> and K</span><sup><span style="font-family:Verdana;">+</span></sup><span style="font-family:Verdana;"> concentrations, relative water content and chlorophyll content. Data from both experiments were subjected to Analysis of variance test using the GenStat software 10</span><sup><span style="font-family:Verdana;">th</span></sup><span style="font-family:Verdana;"> edition and the means separated using least significant difference test. Individual stress response index (ISRI) was calculated for each parameter and the means used in grouping the varieties. Of the genotypes evaluated, four (FARO 44, NERICA 2, NERICA 8 and NERICA 5) were identified as tolerant, two (NERICA 4 and FARO 57) as moderately tolerant, while the rest were found to be sensitive to drought. Equally, two varieties (FARO 44 and RAM 137) stood out in the salinity screening as tolerant varieties, five were moderately tolerant while four (FARO 64, FARO 52, NERICA 2 and FARO 55) were clearly susceptible. FARO 44 is the only genotype that </span><span style="font-family:Verdana;">showed </span></span><span style="font-family:Verdana;">tolerance</span><span style="font-family:""> </span><span style="font-family:Verdana;">to both drought and salinity. The identified drought and salinity tolerant rice genotypes from this study can be recommended as genetic sources for future breeding programs for drought and salinity </span><span style="font-family:Verdana;">tolerance</span><span style="font-family:""> </span><span style="font-family:Verdana;">in rice.
文摘This study investigated the effect of fixed height standing-workstation on different people with diverse anthropometry dimensions. Measurements of some anthropometric and physiological parameters are carried out as bases for the calculation of body mass index (BMI) and the determination of the maximum heart rate (HRmax) and aerobic power (VO2max) of individual subject while performing manual cutting operation with hacksaw on fixed vice height of 940 mm. Twenty subjects (S1 to S20) parted 2 mm thick square-pipe of 25 mm × 25 mm. Each subject carried out cutting operation in 5 replicates and their physiological parameters during activities are measured to determine their expended energy (EE) and oxygen consumption rate (VO2). The results showed that subject S4 with BMI of 20.76 kg/m2 has maximum cutting rate of 5.33 stroke/s, while subject S8 with BMI of 23.39 kg/m2 has minimum cutting rate of 0.92 stroke/s. There was a statistically significant effect on the interaction between BMI, EE and Cutting rate, with F = 827.54, P = 0.000, R2 = 0.967 and S = 1.749 units. Subject S11 was discovered to have VO2 (28.54 l/min) and VO2max (24.36 ml/min/kg), with highest value of EE (2.94 kcal/min). Wear rates of 1.86 teeth/s and 9.55 teeth/s have the same energy cost (EE = 0.87 kcal/min) but different cutting time of 36.65 s (S18) and 10.89 s (S20) respectively. This could explain in-part that excess 25.76 s utilized in operation time by subject S18 is responsible for keeping approximately 7.7 teeth intact as regards tool management. EE and Tool Wear Rate in one-way analysis of variance, were statistically significant (F = 45.87, P = 0.000, R2 = 54.69% and S = 1.617 units) at 0.05 level.
文摘Rose (Rosa hybrida L.) is one of the most important specialty cut flowers produced. Characterization of physiological variability in photosynthetic efficiencies, respiration rate and chlorophyll content is one of the greatest challenges in assessing rose net primary production of flowering, quality of cut flowers and other rose uses. Two modern rose varieties Floribunda and Hybrid Tea were used to optimize different methods for analysing physiological characteristics (photosynthesis rate, respiration rate and chlorophyll content). Many parameters were optimized, five different lights intensity were used (600, 900, 1200, 1500 and 2000 μmol·m-2·s-1) of which 1200 μmol·m-2·s-1 gave the highest photosynthesis rate. Five different measuring times were used (8 am, 10 am, 12 pm, 2 pm and 4 pm) and 12 pm was shown to be the optimum time for measuring photosynthesis rate. Among two different weather conditions (sunny day and cloudy day) sunny day was selected. For respiration rate two different measuring times (1 and 2 hours) after darkness were studied and 1 hour was chosen. Among three different times (10 am, 12 pm and 2 pm) for measuring chlorophyll content, 12 pm was selected. Using these optimized variables will allow researchers to collect robust and reproducible results to be obtained from different studies, and in turn lead to improved yields of horticultural plants.
基金funded by the National Key Research and Development Program of China(2016YFC0400206-04,2017YFC0505304)the National Natural Science Foundation of China(51309016)+1 种基金Central Public-interest Scientific Institution Basal Research Fund(CKSF2016007/TB)Changjiang River Scientific Research Institute Innovation Team(CKSF2017064/NS)
文摘Exploring the response differences of leaf physiology parameters to enhanced nitrogen deposition between saplings and trees is vital for predicting the variations of terrestrial ecosystem structure and function under future global climate change. In this study, the ecophysiological parameters of saplings and trees of Fraxinus mandshurica Rupr. were measured at different levels of nitrogen addition in a temperate forest. The results show that ecophysiological parameters maximum net photosynthetic rate(Pmax), apparent quantum efficiency(a), dark respiration(Rd), light saturation point(Lsp), photosynthetic nitrogen use efficiency(PNUE),specific leaf area(SLA)and stomatal conductance under saturated light intensity(Gsmax) were higher in saplings than in trees. These physiological parameters and not Nleaf(leaf nitrogen content)led to relatively lower Pmaxand Rdin trees. For both saplings and trees, low and median nitrogen addition(23 and 46 kg ha-1a-1) resulted in significant increases in Pmax, Rd, Lsp, Chl, PNUE, SLA and Gsmax. These parameters tended to decline under high additions of nitrogen(69 kg ha-1a-1),whereas Nleaf was always enhanced with increasing nitrogen. Variations in Pmax and Rd with increasing nitrogen were attributed to variations in the strongly related parameters of, Lsp, Chl, PNUE, SLA and Gsmax. Overall, the response sensitivity of physiological parameters to enhanced nitrogen levels was lower in trees compared with saplings.
文摘Objective:To analyse the seasonal effect on physiological parameters, reproductive profiles andin vitro fertility in breeding mithun bulls.Methods: A total of ten adult mithun bulls age of 5 to 6 years old with good body condition (score 5-6) were selected from ICAR-NRC on Mithun, Jharnapani, Nagaland, India. The seasons were categorised into winter, spring, summer and autumn seasons based on the meteorological data and sunshine hours. The physiological parameters, reproductive profiles andin vitro fertility parameters were assessed during different seasons in mithun under the semi-intensive system of management.Results:The statistical analysis revealed that these experimental parameters were differed significantly (P<0.05) among the seasons and in overall spring and winter seasons were more beneficial in mithun breeding programme, although, the breeding in mithun occurred throughout the year with variation.Conclusions:It is concluded that collection & preservation of mithun semen and artificial insemination in mithun species during the season of spring and winter has significant beneficial effect in terms of semen production, freezability and fertility for artificial breeding programme in mithun under the semi-intensive system.
基金This research was funded by the National Key Research and Development Program of China(2018YFD0100600)the Special Project for Construction of Modern Agricultural Industrial Technology System(CARS-12).
文摘Understanding the mechanisms of drought resistance in crop species is crucial for the selection and breeding of tolerant rapeseed(Brassica napus L.)varieties.The present study aimed to assess the physiological and anatomical responses of two rapeseed genotypes,P287(drought-tolerant)and T88(drought-sensitive)under three intensities of drought stress.All physiological and anatomical parameters related to drought acclimation were significantly altered in both genotypes under stress conditions.At the fourth-leaf stage,the relative water content,chlorophyll content,protein content,malondialdehyde content,and the activities of peroxidase and catalase in P287 were significantly higher than those in T88,particularly under severe drought conditions.After rehydration,all physiological indexes recovered rapidly,especially in P287.In addition,under drought stress,compared with T88,P287 had thicker palisade tissue,thinner spongy tissue,higher ratio of chloroplast length to chloroplast width,higher stomatal density and stomatal closure rate.Overall,the interaction between physiological and anatomical features improved the drought tolerance of P287 under drought stress conditions.
文摘Split-root solution culture was used to study the promoting effect of lanthanum on rice (Oryza sativa) growth and its physiological mechanisms. Results sho w that low concentration (0.05~1.5 mg·L -1) increases rice yield an d grain numbers. High concentration depresses grain formation (9~30 mg·L -1 ) and root elongation (1.5~30 mg·L -1). No significant influence on str aw dry weight was found over the whole concentration range except the 0.05 mg·L -1 treatment. With the increase of La concentration from 0.05 to 0.75 mg· L -1, catalase (CAT) activity in the first fully expandeing leaves and root s decreases. When La concentration is greater than 0.75 mg·L -1 or less than 9 mg·L -1, it significantly decreases superoxide dismutase activity ( SOD) in the leaves and roots. No significant effects were found on chlorophyll, protein and malondialdehyde (MDA) content. Possible mechanisms of La′s promotin g effect on rice growth and reduction effect of ·O- 2 were discussed.
基金Supported by the Society Development Program of the Natural Science Foundation of Jiangsu Province in China (No.BS2002016)
文摘Heavy metal pollution has become a worldwide problem in aquaculture. We studied copper (Cu2+ ) accumulation and physiological responses of two red algae Gracilaria lemaneiformis and Gracilaria lichenoides from China under Cu2+ exposure of 0-500 μg/L in concentration. Compared with G. lemaneiformis, G. lichenoides was more capable in accumulating Cu2+ , specifically, more Cu2+ on extracellular side (cell wall) than on intracellular side (cytoplasm) and in cell organelles (especially chloroplast, cell nucleus, mitochondria, and ribosome). In addition, G. lichenoides contained more insoluble polysaccharide in cell wall, which might promote the extracellular Cu2+ -binding as an efficient barrier against metal toxicity. Conversely, G. lemaneiformis was more vulnerable than G. lichenoides to Cu 2+ toxin for decreases in growth, pigment (chlorophyll a, chlorophyll b, phycobiliprotein, and β-carotene) content, and photosynthetic activity. Moreover, more serious oxidative damages in G. lemaneiformis than in G. lichenoides, in accumulation of reactive oxidative species and malondialdehyde, and in electrolyte leakage, because of lower antioxidant enzyme (superoxide dismutase and glutathione reductase) activities. Therefore, G. lichenoides was less susceptible to Cu2+ stress than G. lemaneiformis.
基金funded by Mississippi Rice Promotion Board and United States Department of AgricultureNational Institute of Food and Agriculture(USDA-NIFA,Grant No.2013-34263-20931)sub-award to Mississippi State University(Grant No.G-7799-2)
文摘The majority of rice(Oryza sativa L.) produced in the southern USA is drill-seeded and grown under upland-like conditions because permanent flooding is established after the four-leaf stage. Therefore, rice during the seedling growth stage will be subjected to variable soil moisture content. A greenhouse experiment was conducted to evaluate the performance of 15 rice cultivars commonly grown in Mississippi of USA under early-season soil moisture stress. Twenty morpho-physiological parameters of rice seedlings subjected to three different levels(100%, 66% and 33% field capacity) of soil moisture, from 10 to 30 d after sowing, were measured. Significant moisture stress × treatment interaction(P < 0.001) was observed for most of the parameters. Further, the total drought response index(TDRI) was developed to score the cultivars for drought tolerance with the variation from 26.88 to 36.21. Accordingly, the cultivars were classified into different groups of tolerance. The cultivars CL152 and CL142-AR were classified as the least and the most tolerant to drought based on TDRI and standard deviation, respectively. Even though both total root(R^2 = 0.98) or shoot(R^2 = 0.76) drought responses indices were positively correlated with TDRI, root traits were important in deriving the indices. Therefore, TDRI could be used to select cultivars for drought tolerance in a given environment and develop rice varieties with early-season drought tolerance. However, further research is needed to identify and characterize tolerance at other stages to assist breeding programs in rice.