The sequence fragment of PHYA, obtained from transcriptome sequencing,was used as the template, and the full-length c DNA sequence of PHYA gene in tall fescue was amplified using 3＇RACE and 5＇RACE techniques. The c ...The sequence fragment of PHYA, obtained from transcriptome sequencing,was used as the template, and the full-length c DNA sequence of PHYA gene in tall fescue was amplified using 3＇RACE and 5＇RACE techniques. The c DNA sequence of PHYA gene has a complete open reading frame（ORF, 293-6 682 bp）, and it encodes a protein composed of 1 129 amino acids. The N-terminal of Fa PHYA is composed of GAF and Phytochrome domains, and its C-terminal contains two repeated PAS domains, one histidine kinase A domain and one histidine kinase-like ATPase domain. The homology analysis showed that the amino acid sequences of Fa PHYA of tall fescue and PHYAs of gramineous plants have higher homologies（85%）, indicating close genetic relationships. However, the homologies between FaPHYA of tall fescue and PHYAs of monocotyledons are lower, indicating far genetic relationships.展开更多
Phytochrome family mainly senses red and far-red light to regulate a range of developmental processes throughout the life cycle of plants. Rice phytochrome gene family is composed of three members known as PHYA, PHYB ...Phytochrome family mainly senses red and far-red light to regulate a range of developmental processes throughout the life cycle of plants. Rice phytochrome gene family is composed of three members known as PHYA, PHYB and PHYC. It has been elucidated that individual phytochromes display both unique and overlapping roles in rice photomorphogenesis by characterization of all rice phytochrome mutants including single mutants, all combinations of double mutants as well as triple mutants. Based on the published data and authors’ ongoing studies, current knowledge of rice phytochrome functions in regulating seedling de-etiolation, root gravitropic response and elongation, plant architecture, flowering time and fertility is summarized. Additionally, the important issues in the field of rice phytochromes are proposed.展开更多
基金Supported by National Natural Science Foundation of China(31360576)~~
文摘The sequence fragment of PHYA, obtained from transcriptome sequencing,was used as the template, and the full-length c DNA sequence of PHYA gene in tall fescue was amplified using 3＇RACE and 5＇RACE techniques. The c DNA sequence of PHYA gene has a complete open reading frame（ORF, 293-6 682 bp）, and it encodes a protein composed of 1 129 amino acids. The N-terminal of Fa PHYA is composed of GAF and Phytochrome domains, and its C-terminal contains two repeated PAS domains, one histidine kinase A domain and one histidine kinase-like ATPase domain. The homology analysis showed that the amino acid sequences of Fa PHYA of tall fescue and PHYAs of gramineous plants have higher homologies（85%）, indicating close genetic relationships. However, the homologies between FaPHYA of tall fescue and PHYAs of monocotyledons are lower, indicating far genetic relationships.
基金supported by the grants from the Chinese National Natural Science Foundations (Grant Nos. 30870192 and 30971744)the National Major Science and Technology Project to Create New Crop Varieties Using Gene Transfer Technical (Grant No. 2009ZX08001-029B)the Open Research Program from the Key Laboratory of Crop Biology, China (Grant No. 2009KF04)
文摘Phytochrome family mainly senses red and far-red light to regulate a range of developmental processes throughout the life cycle of plants. Rice phytochrome gene family is composed of three members known as PHYA, PHYB and PHYC. It has been elucidated that individual phytochromes display both unique and overlapping roles in rice photomorphogenesis by characterization of all rice phytochrome mutants including single mutants, all combinations of double mutants as well as triple mutants. Based on the published data and authors’ ongoing studies, current knowledge of rice phytochrome functions in regulating seedling de-etiolation, root gravitropic response and elongation, plant architecture, flowering time and fertility is summarized. Additionally, the important issues in the field of rice phytochromes are proposed.
