期刊文献+
共找到4篇文章
< 1 >
每页显示 20 50 100
青鱼生长激素在毕赤酵母中的表达 被引量:4
1
作者 谢帝芝 刘臻 +3 位作者 王赏初 张建设 肖调义 鲁双庆 《淡水渔业》 CSCD 北大核心 2011年第6期19-24,共6页
根据GenBank数据库中登记的青鱼(Mylopharyngodon picens)生长激素(bcGH)基因序列(AF389238)设计特异性引物,采用RT-PCR技术扩增青鱼生长激素cDNA编码区,并通过定向插入含强启动子AOX I的表达载体pPIC3.5k、电击转入毕赤酵母GS115菌株、... 根据GenBank数据库中登记的青鱼(Mylopharyngodon picens)生长激素(bcGH)基因序列(AF389238)设计特异性引物,采用RT-PCR技术扩增青鱼生长激素cDNA编码区,并通过定向插入含强启动子AOX I的表达载体pPIC3.5k、电击转入毕赤酵母GS115菌株、G418多克隆子筛选和测序验证等步骤进行了重组生长激素酵母菌诱导表达等相关实验,成功获得重组生长激素酵母菌(pPIC3.5k-bcGH重组酵母菌)。蛋白表达产物经SDS-PAGE和ELISA检测,在23 kD处发现一条与预期蛋白分子量大小一致的特异性条带,并且目的蛋白能与青鱼生长激素多克隆抗体特异性结合,说明bcGH在酵母胞内得到正确表达。表达条件优化过程中发现,诱导24 h已有重组蛋白表达,pH值4.5~7.0之间都能表达重组蛋白。在pH值6.5、0.5%甲醇、温度30℃和培养时间72 h的优化条件下,目的蛋白的表达量高达893 mg/L。 展开更多
关键词 青鱼(Mylopharyngodon picens) 生长激素 毕赤酵母 表达
下载PDF
浅议建设工程规划验收制度 被引量:1
2
作者 邢翔 夏利芬 《规划师》 2005年第9期73-75,共3页
《广州市城市规划条例》第四十四条明确规定了建设工程规划验收制度。它的作用是发现,制止和处理违法建设,落实公共建筑配套项目,实施环境建设。它是一种行政许可行为,是合乎法律规定的,其合法性不应受到质疑。
关键词 城市规划 规划验收 行政许可
下载PDF
Imaging Molecular Orbitals of Single Picene Molecules Adsorbed on Cu(111) Surface: a Combined Experimental and Theoretical Study
3
作者 Chun-shengZhou Huan Shan +1 位作者 Bin Li Ai-di Zhao 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2017年第1期29-35,I0001,共8页
Picene, which attracts the great interest of researchers, not only can be used to fabricate thin film transistors with high hole mobilities, but also is the parent material of a new type organic superconductor. Here, ... Picene, which attracts the great interest of researchers, not only can be used to fabricate thin film transistors with high hole mobilities, but also is the parent material of a new type organic superconductor. Here, we investigate the electronic properties of individual picene molecules directly adsorbed on Cu(111) surface by a combination of experimental scanning tunneling microscopy/spectroscopy measurements and theoretical calculations based on the density functional theory. At low coverage, the picene molecules exhibit mono-dispersed adsorption behavior with the benzene ring planes parallel to the surface. The highest occupied state around -1.2 V and the lowest unoccupied state around 1.6 V with an obvious energy gap of the singly adsorbed picene molecule are identified by the dI/dV spectra and maps. In addition, we observe the strong dependence of the dI/dV signal of the unoccupied states on the intramolecular positions. Our first-principles calculations reproduce the above experimental results and interpret them as a specific molecule-substrate interaction and energy/spatial distributions of hybrid states mainly derived from different molecular orbitals of picene with some intermixing between them. This work provides direct information on the local electronic structure of individual picene on a metallic substrate and will facilitate the understanding the dependence of electron transport properties on the coupling between molecules and metal electrodes in single-molecule devices. 展开更多
关键词 Scanning tunneling microscopy Picene Molecular orbitals Density functional theory
下载PDF
NBO/NMR Analysis of Interaction between Picene and Boron Nitrid Nano Ring
4
作者 MEHRNOOSH Khaleghian 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2019年第7期1116-1122,共7页
In order to investigate the non-bonded interaction between picene and B12 N12 nano ring(BNNR) in different orientations and distances, we focused on a single-wall boronnitride B12 N12 nano-ring. The geometry of molecu... In order to investigate the non-bonded interaction between picene and B12 N12 nano ring(BNNR) in different orientations and distances, we focused on a single-wall boronnitride B12 N12 nano-ring. The geometry of molecules was optimized using the B3 LYP method with 6-31 g* basis set. The reactivity and stability of picene as an aromatic compound alone and upon exposure to BNNR were checked. Then NBO, FREQ, NICS and adsorption energy of picene alone and in the presence of BNNR Field were derived to estimate the picene structural stability.The results of any order were found to reduce the reactivity and increase the stability for picene in the presence BNNR field. 展开更多
关键词 picene DFT Freq NBO NICS
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部