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可诱导型敲低PICH的MDA-MB-231细胞系的构建及鉴定
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作者 李万金 黄燕 +3 位作者 吕璐冶 韩秋影 周涛 陈亮 《生物技术通讯》 CAS 2019年第2期153-157,共5页
目的:构建稳定表达可诱导型polo样激酶1相关检查点解旋酶(PICH)短发夹RNA(shRNA)的三阴乳腺癌细胞系MDA-MB-231,并通过检测加入诱导剂后细胞的增殖评价敲低效果。方法:设计并合成2条特异靶向PICH的shRNA序列,用分子克隆技术构建pLKO.1-t... 目的:构建稳定表达可诱导型polo样激酶1相关检查点解旋酶(PICH)短发夹RNA(shRNA)的三阴乳腺癌细胞系MDA-MB-231,并通过检测加入诱导剂后细胞的增殖评价敲低效果。方法:设计并合成2条特异靶向PICH的shRNA序列,用分子克隆技术构建pLKO.1-tet-on-shPICH-Puromycin质粒,经菌落PCR及基因测序验证;慢病毒包装并感染细胞,通过抗性筛选获得嘌呤霉素抗性的细胞;经强力霉素(DOX)诱导表达后,Western印迹检测PICH的敲低效果,并通过MTT法对细胞增殖进行检测。结果:菌落PCR凝胶电泳及DNA测序结果显示pLKO.1-tet-on-shPICHPuromycin质粒构建成功;Western印迹结果表明,在DOX诱导下,MDA-MB-231细胞中的PICH蛋白质表达下降,表明可诱导型PICH稳定敲低细胞系构建成功;进一步实验结果显示,MDA-MB-231细胞的增殖随PICH的表达下调而受到显著抑制。结论:构建了可诱导稳定敲低PICH的三阴乳腺癌细胞系MDA-MB-231,为进一步研究PICH在三阴乳腺癌中的作用以及具体的分子机制奠定了实验基础。 展开更多
关键词 polo样激酶1相关检查点解旋酶(pich) 三阴乳腺癌 可诱导稳定敲低 细胞增殖
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Modeling and Free Vibration Behavior of Rotating Composite Thin-walled Closed-section Beams with SMA Fibers 被引量:4
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作者 REN Yongsheng YANG Shulian DU Xianghong 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2012年第5期1029-1043,共15页
Smart structure with active materials embedded in a rotating composite thin-walled beam is a class of typical structure which is using in study of vibration control of helicopter blades and wind turbine blades. The dy... Smart structure with active materials embedded in a rotating composite thin-walled beam is a class of typical structure which is using in study of vibration control of helicopter blades and wind turbine blades. The dynamic behavior investigation of these structures has significance in theory and practice. However, so far dynamic study on the above-mentioned structures is limited only the rotating composite beams with piezoelectric actuation. The free vibration of the rotating composite thin-walled beams with shape memory alloy(SMA) fiber actuation is studied. SMA fiber actuators are embedded into the walls of the composite beam. The equations of motion are derived based on Hamilton's principle and the asymptotically correct constitutive relation of single-cell cross-section accounting for SMA fiber actuation. The partial differential equations of motion are reduced to the ordinary differential equations of motion by using the Galerkin's method. The formulation for free vibration analysis includes anisotropy, pitch and precone angle, centrifugal force and SMA actuation effect. Numerical results of natural frequency are obtained for two configuration composite beams. It is shown that natural frequencies of the composite thin-walled beam decrease as SMA fiber volume and initial strain increase and the decrease in natural frequency becomes more significant as SMA fiber volume increases. The actuation performance of SMA fibers is found to be closely related to the rotational speeds and ply-angle. In addition, the effect of the pitch angle appears to be more significant for the lower-bending mode ones. Finally, in all cases, the precone angle appears to have marginal effect on free vibration frequencies. The developed model can be capable of describing natural vibration behaviors of rotating composite thin-walled beam with active SMA fiber actuation. The present work extends the previous analysis done for modeling passive rotating composite thin-walled beam. 展开更多
关键词 free vibration thin-walled composite beams shape memory alloy rotating beams pich angle precone angle
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Predicting Reference Evaporation for the Ethiopian Highlands
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作者 Anwar A. Adem Dessalew W. Aynalem +1 位作者 Seifu A. Tilahun Tammo S. Steenhuis 《Journal of Water Resource and Protection》 2017年第11期1244-1269,共26页
Water is likely the most limiting factor in increasing agricultural production in large parts of Africa. Reference evaporation (ET0) is a key hydrological parameter to use efficiently the scarce supply. Several method... Water is likely the most limiting factor in increasing agricultural production in large parts of Africa. Reference evaporation (ET0) is a key hydrological parameter to use efficiently the scarce supply. Several methods are available for predicting reference evaporation, but the accuracy of any of the methods has not been established for the Ethiopian highlands. The objective of this study is, therefore, to select the best methods for calculating the reference evaporation ET0. For the section, meteorological data of the Bahir Dar station were used, because all data needed for this study including the Class A pan Evaporation were recorded on a daily basis. Pan evaporation was considered as the best estimator of the reference evaporation. The results showed that the FAO-Penman Monteith (using solar radiation, wind speed, temperature and relative humidity) and Enku method (using only maximum daily temperatures) have acceptable daily ET0 ranges and predicted to Class A pan evaporation with correlation coefficients greater than 90% in a monthly basis. Next best was the Thornthwaite’s method with correlation coefficient of 89% with pan evaporation. Piche methods performed relatively well with correlation coefficient of greater than 70%. Blaney-Criddle, Priestley & Taylor, and Hargreaves performed the poorest in predicting pan evaporation. These methods should be recalibrated for local condition and therefore not recommended for use in the Ethiopian highlands. In summary, the FAO-Penman Monteith is recommended for locations where the input data are available;otherwise, the Enku method using maximum daily temperature is best for estimating the reference evaporation. 展开更多
关键词 PAN piche AFRICA Potential EVAPORATION FAO-56
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引入非线性有限元法的同步带传动最佳节距差计算方法
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作者 丁渭平 《机械设计与制造》 北大核心 1999年第6期34-36,共3页
建立了求解同步带传动带与带轮节距差最佳值的数字模型,并对模型中所涉及到的非线性有限元问题进行了分析,从而形成一种引入非线性有限元法的同步带传动最佳节距差计算方法。通过具体算例,并将算例结果用于实验,证明了该方法的可靠性。
关键词 同步带传动 最佳节距差 非线性有限元
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短跑中“扒地”技术动作的生物力学分析与若干思考 被引量:4
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作者 林辉杰 李兵 《安徽体育科技》 2005年第2期47-49,共3页
“扒地”技术动作能否使前支撑阶段产生动力是一直被研究人员关注的重要课题。通过综合前人对“扒地”技术动作的分析结论,进一步深入分析和提出若干思考,旨在为深入了解前支撑阶段的“扒地”技术动作提供参考。
关键词 技术动作 若干思考 生物力学分析 扒地 短跑 重要课题 研究人员 支撑
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塔东顺南1井区火山活动与断裂及热液作用关系 被引量:3
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作者 李坤白 云露 +1 位作者 蒲仁海 曹自成 《地球物理学进展》 CSCD 北大核心 2016年第5期1934-1946,共13页
塔里木盆地虽广泛分布一到两期二叠系火山岩,但它们何时尖灭、如何尖灭、与奥陶系碳酸盐岩热液储层及断裂活动有无关系等均不十分清楚.在塔里木盆地东部顺南1井三维地震区内发育火山岩,且在奥陶系发现可能的热液储层气藏.本文根据钻井... 塔里木盆地虽广泛分布一到两期二叠系火山岩,但它们何时尖灭、如何尖灭、与奥陶系碳酸盐岩热液储层及断裂活动有无关系等均不十分清楚.在塔里木盆地东部顺南1井三维地震区内发育火山岩,且在奥陶系发现可能的热液储层气藏.本文根据钻井和三维地震仔细解释了火山岩的岩性、期次、展布、火山口位置和沉积尖灭线等,认为工区内二叠系只发育了一期库普库兹曼组玄武岩及凝灰岩,开派雷兹克组为陆源碎屑岩,周边顺南2井、古隆1井火山岩的缺失属于沉积缺失,非剥蚀缺失.火山口虽然分布在过顺南4井的北北东断裂两侧,但其位置也受早期NEE和NW向断裂带与晚期NNE向断裂交汇点的控制.此外,顺南1井工区奥陶系地层发育大量由热液溶蚀造成的片状强振幅异常以及管状强振幅异常,其中片状强振幅的分布与火山口位置有一定相似性,即受到多组断裂交汇以及断裂密集程度的控制. 展开更多
关键词 火山岩尖灭 火山期次 火山口 断裂 热液储层
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古莱州方言“去分阴阳”——基于对共时材料的统计分析 被引量:2
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作者 孙克敏 《语言研究》 CSSCI 北大核心 2016年第3期48-54,共7页
莱州方言的去声曾分化为阴去和阳去两个调类。依据内部证据,推断出"去分阴阳"的形成时间应早于阳去与阳平合流,也早于"次浊入归去"。
关键词 胶辽官话 去分阴阳 轻声前调 相关系数
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