Objective: To study the effects of butylphthalide on bronchial asthma in guinea pigs, and investigate the involvement of endothelin. Methods: In guinea pigs, bronchial asthma was induced by injection of ovalbumin(OVA)...Objective: To study the effects of butylphthalide on bronchial asthma in guinea pigs, and investigate the involvement of endothelin. Methods: In guinea pigs, bronchial asthma was induced by injection of ovalbumin(OVA) and provoked by inhalation of OVA, and the effects of butylphthalide on asthma were evaluated through the changes it induced by OVA, pulmonary function, endothelin-1(ET-1) contents and activity of endothelin converting enzyme-1(ECE-1) in bronchoalveolar lavage fluid(BALF), serum and lung tissue, and the gene expression of ET-1 in lung tissue. Results: Butylphthalide significantly improved pulmonary function, lowered asthmatic behavior score, inhibited the activity of ECE-1, and reduced ET-1 gene expression level in lung tissue. Conclusion: Butylphthalide has an anti-asthma effect and the mechanisms involve inhibition of ECE-1 activity and lowering of ET-1geng expression.展开更多
Guinea pig model of asthma was established with the sensitization of the animals with aerosolized ovalbumin. A group of asthmatic guinea pigs were treated with tripholide. It was found that the airway responsiveness t...Guinea pig model of asthma was established with the sensitization of the animals with aerosolized ovalbumin. A group of asthmatic guinea pigs were treated with tripholide. It was found that the airway responsiveness to histamine was markedly higher in the asthmatic group than in the control. The total cell count, absolute eosinophil count, percentage of hypodense eosinophils and the level of eosinophil viability enhancing activity in the bronchoalveolar lavage fluid (BALF) were significantly higher in the asthmatic group than in the control. The percentage of hypodense eosinophils and absolute eosinophil count were in close correlation with airway responsiveness (r= -0. 76 and - 0. 88, P <0. 05 and 0. 01 respectively). Treatment of the asthmatic guinea pigs with tripholide (8 μg/kg, given orally for 7 d) significantly reduced the airway responsiveness and decreased the percentage of hypodense eosinophils and the level of eosinophil viability enhancing activity but eosinophilia in BALF was not affected. It was concluded that tripholide exerts its effects to decrease airway responsiveness in asthma through its action to reduce active hypodense eosinophils.展开更多
Objective To investigate the effects of antihistamine agents, loratadine and ketotifen, on the model of exercise-induced asthma in guinea pigs with lipopolysaccharide （LPS） and metyapone. Methods Nineteen guinea pig...Objective To investigate the effects of antihistamine agents, loratadine and ketotifen, on the model of exercise-induced asthma in guinea pigs with lipopolysaccharide （LPS） and metyapone. Methods Nineteen guinea pigs were injected peritoneolly with LPS （ 1mg/kg, i. p. ） and metyapone （ 50mg/kg, i. p. ）. Then they were randomized into 3 groups. The control group （ n = 7） did not take any drug, ketotifen group （ n = 6） administered orally ketotifen （ Img·kg^-1· d^-1 ） and loratadine group （ n = 6） administered orally loratadine （ 2mg·kg^-1·d^-1 ） for 4 days. On thefifth day, lung resistance （RL） and dynamic compliance of respiratory system （Cdyn） of 3 groups were measured before and after exercise challenge. The total number of leukocytes in bronchoalveolar lavage fluid （BALF） from 3 groups was counted and differentiated cell type count was measured. Morphometric examination of the animal lungs was performed. Results In control group, RLincreased and Cdyn decreased significantly after exercise challenge. In ketotifen group and loratadine group with no change. There was a significant increase in the number of neutrophils, macrophages and eosinophils in BALF from control group. The infiltration of neutrophils in the bronchial mucosa was shown in control group in the morphometric study. Conclusion Loratadine and ketotifen can inhibit the exercise-induced bronchoconstriction in guinea pigs pretreated with LPS and metyrapone. Inhibiting the formation of inflammation in airway may be the therapeutic mechanism of these H-I receptor antagonists.展开更多
To investigate the dynamic changes of eosinophilic accumulation and apoptosis in the airway of asthmatic guinea pigs and explore the significance of eosinophilic apoptosis in eosinophilia. Methods: we made a detailed ...To investigate the dynamic changes of eosinophilic accumulation and apoptosis in the airway of asthmatic guinea pigs and explore the significance of eosinophilic apoptosis in eosinophilia. Methods: we made a detailed examination of the histology, cytology and apoptosis in the airway up to 7 d after antigen challenge. Apoptosis was assessed with in situ tailing. Results: The findings were as follows: ① After antigen challenge, eosinophil infiltration in the bronchi was the most serious from & to 24 h and then declined. ② Eosinophilia was remarkable in bronchoalveolar lavage fluid (BALF) and bronchial tissue at 8 h,peaked during 24 to 72 h and persisted for 7 days at least. ③ Eosinophilia was accompanied by epithelia damage and subepithelial edema. ④ The apoptosis rate of eosinophils in BALF was significantly lower in the asthmatic guinea pigs than in the normal controls (P<0. 05 ). Conclusion: These findings suggest that the apoptosis of eosinophils in asthmatic guinea pigs is inhibited, which may contribute to protracted course of eosinophilia in the airway.展开更多
基金supported by the National Natural Science Fund (81460668)the Natural Science Foundation of Gansu Province (1310RJZA086, 1606RJZA011)the open fund project of Key Laboratory of Pharmacology and Toxicology for Traditional Chinese Medicine of Gansu Province (ZDSYS-KJ-2015-002)
文摘Objective: To study the effects of butylphthalide on bronchial asthma in guinea pigs, and investigate the involvement of endothelin. Methods: In guinea pigs, bronchial asthma was induced by injection of ovalbumin(OVA) and provoked by inhalation of OVA, and the effects of butylphthalide on asthma were evaluated through the changes it induced by OVA, pulmonary function, endothelin-1(ET-1) contents and activity of endothelin converting enzyme-1(ECE-1) in bronchoalveolar lavage fluid(BALF), serum and lung tissue, and the gene expression of ET-1 in lung tissue. Results: Butylphthalide significantly improved pulmonary function, lowered asthmatic behavior score, inhibited the activity of ECE-1, and reduced ET-1 gene expression level in lung tissue. Conclusion: Butylphthalide has an anti-asthma effect and the mechanisms involve inhibition of ECE-1 activity and lowering of ET-1geng expression.
文摘Guinea pig model of asthma was established with the sensitization of the animals with aerosolized ovalbumin. A group of asthmatic guinea pigs were treated with tripholide. It was found that the airway responsiveness to histamine was markedly higher in the asthmatic group than in the control. The total cell count, absolute eosinophil count, percentage of hypodense eosinophils and the level of eosinophil viability enhancing activity in the bronchoalveolar lavage fluid (BALF) were significantly higher in the asthmatic group than in the control. The percentage of hypodense eosinophils and absolute eosinophil count were in close correlation with airway responsiveness (r= -0. 76 and - 0. 88, P <0. 05 and 0. 01 respectively). Treatment of the asthmatic guinea pigs with tripholide (8 μg/kg, given orally for 7 d) significantly reduced the airway responsiveness and decreased the percentage of hypodense eosinophils and the level of eosinophil viability enhancing activity but eosinophilia in BALF was not affected. It was concluded that tripholide exerts its effects to decrease airway responsiveness in asthma through its action to reduce active hypodense eosinophils.
文摘Objective To investigate the effects of antihistamine agents, loratadine and ketotifen, on the model of exercise-induced asthma in guinea pigs with lipopolysaccharide （LPS） and metyapone. Methods Nineteen guinea pigs were injected peritoneolly with LPS （ 1mg/kg, i. p. ） and metyapone （ 50mg/kg, i. p. ）. Then they were randomized into 3 groups. The control group （ n = 7） did not take any drug, ketotifen group （ n = 6） administered orally ketotifen （ Img·kg^-1· d^-1 ） and loratadine group （ n = 6） administered orally loratadine （ 2mg·kg^-1·d^-1 ） for 4 days. On thefifth day, lung resistance （RL） and dynamic compliance of respiratory system （Cdyn） of 3 groups were measured before and after exercise challenge. The total number of leukocytes in bronchoalveolar lavage fluid （BALF） from 3 groups was counted and differentiated cell type count was measured. Morphometric examination of the animal lungs was performed. Results In control group, RLincreased and Cdyn decreased significantly after exercise challenge. In ketotifen group and loratadine group with no change. There was a significant increase in the number of neutrophils, macrophages and eosinophils in BALF from control group. The infiltration of neutrophils in the bronchial mucosa was shown in control group in the morphometric study. Conclusion Loratadine and ketotifen can inhibit the exercise-induced bronchoconstriction in guinea pigs pretreated with LPS and metyrapone. Inhibiting the formation of inflammation in airway may be the therapeutic mechanism of these H-I receptor antagonists.
文摘To investigate the dynamic changes of eosinophilic accumulation and apoptosis in the airway of asthmatic guinea pigs and explore the significance of eosinophilic apoptosis in eosinophilia. Methods: we made a detailed examination of the histology, cytology and apoptosis in the airway up to 7 d after antigen challenge. Apoptosis was assessed with in situ tailing. Results: The findings were as follows: ① After antigen challenge, eosinophil infiltration in the bronchi was the most serious from & to 24 h and then declined. ② Eosinophilia was remarkable in bronchoalveolar lavage fluid (BALF) and bronchial tissue at 8 h,peaked during 24 to 72 h and persisted for 7 days at least. ③ Eosinophilia was accompanied by epithelia damage and subepithelial edema. ④ The apoptosis rate of eosinophils in BALF was significantly lower in the asthmatic guinea pigs than in the normal controls (P<0. 05 ). Conclusion: These findings suggest that the apoptosis of eosinophils in asthmatic guinea pigs is inhibited, which may contribute to protracted course of eosinophilia in the airway.
