目的:为证明膀胱二聚体Cajal间质细胞(interstitial cells of Cajal,ICC)发挥起搏作用提供超微结构和功能学证据。方法:电子显微镜下鉴定组织和培养的豚鼠膀胱二聚体ICC并观察ICC之间的超微联系;体外分离、培养豚鼠膀胱ICC,运用细胞膜...目的:为证明膀胱二聚体Cajal间质细胞(interstitial cells of Cajal,ICC)发挥起搏作用提供超微结构和功能学证据。方法:电子显微镜下鉴定组织和培养的豚鼠膀胱二聚体ICC并观察ICC之间的超微联系;体外分离、培养豚鼠膀胱ICC,运用细胞膜片钳技术观察ATP(100μmol/L)刺激下二聚体ICC和单体ICC的兴奋性差别。结果:二聚体ICC中两个ICC细胞胞体紧密相连,细胞之间的间隙小于20 nm,存在大量缝隙连接,局部细胞膜融合,形成联胞体。在ATP(100μmol/L)的刺激下体外培养的二聚体ICC的内向电流幅度为286.9±26.4 pA,显著大于单体ICC的内向电流幅度163.8±18.6 pA(P<0.01)。结论:二聚体ICC具有形成高兴奋起搏电流的超微结构基础,高兴奋性的二聚体ICC可能才是逼尿肌的起搏细胞。展开更多
With the popularity of urine flow dynamic monitoring and indwelling catheter technologies in animal experiments,the urethral catheterization has become a common technique in scientific research.The miniature pig is co...With the popularity of urine flow dynamic monitoring and indwelling catheter technologies in animal experiments,the urethral catheterization has become a common technique in scientific research.The miniature pig is considered as one of the major animal species used in scientific research and is increasingly being used as an alternative to a dog or monkey as the nonrodent species of choice in the preclinical toxicological testing of pharmaceuticals.[1] Since there are three acute angles bending in the urethra of the male miniature pig,and the end of the penis head is cork-screw shaped,it is difficult to implement routine urethral catheterization in male miniature pigs.Currently,researchers usually implement bladder colostomies or dissections in male miniature pigs,while transurethral urethral catheterization can be implemented in female miniature pigs.According to the existing reports,implementing transurethral urethral catheterization in female miniature pigs is seldom seen.[2] We chose 3-month-old female Bama miniature pigs in our experiments and employ the following methods：first,use a ＂V＂-shaped platform with an angle of about 30° to the horizontal plane;second,utilize a pediatric laryngoscope for deep lighting;third,bend the lower limbs and press down to the ventral side.Satisfactory results through transurethral urethral catheterization were achieved in the end.展开更多
基金This study was supported by a grant from the National Natural Science Foundation of China （No. 81271366）.
文摘With the popularity of urine flow dynamic monitoring and indwelling catheter technologies in animal experiments,the urethral catheterization has become a common technique in scientific research.The miniature pig is considered as one of the major animal species used in scientific research and is increasingly being used as an alternative to a dog or monkey as the nonrodent species of choice in the preclinical toxicological testing of pharmaceuticals.[1] Since there are three acute angles bending in the urethra of the male miniature pig,and the end of the penis head is cork-screw shaped,it is difficult to implement routine urethral catheterization in male miniature pigs.Currently,researchers usually implement bladder colostomies or dissections in male miniature pigs,while transurethral urethral catheterization can be implemented in female miniature pigs.According to the existing reports,implementing transurethral urethral catheterization in female miniature pigs is seldom seen.[2] We chose 3-month-old female Bama miniature pigs in our experiments and employ the following methods：first,use a ＂V＂-shaped platform with an angle of about 30° to the horizontal plane;second,utilize a pediatric laryngoscope for deep lighting;third,bend the lower limbs and press down to the ventral side.Satisfactory results through transurethral urethral catheterization were achieved in the end.
