Objectives To investi-gate the effect of electrical stimulation during absolute refractory period on contraction and relaxation of ventricular myocytes from normal guinea - pigs. Methods The guinea - pig ventricular m...Objectives To investi-gate the effect of electrical stimulation during absolute refractory period on contraction and relaxation of ventricular myocytes from normal guinea - pigs. Methods The guinea - pig ventricular myocytes were obtained by enzyme digesting, and the extent of its contraction and relaxation and Ca2+ transient were recorded through the motion edge detection system. Results (1) The contracting extent of guinea - pig ventricular myocytes increased 15.45±6.48% , and the peak velocity of shorting (contraction) and the peak veloci ty of relengthening (relaxation) increased 15.97 ± 8.37% and 21. 63±8. 06% respectively (n = 10); (2) The extent of ventricular myocytes fluorescence ra tio(360/380) (F360/F380) increased 22. 55±9. 08%, and the peak velocity of ventricular myocytes F360 /F380 increased 36. 75 ± 9. 77% and 23. 62±4. 47% during shorting and relengthening respectively (n = 6) . Conclusions Appropriate electrical stimulation may strengthen the contracting and relaxing function of normal guinea - pig ventricular myocytes.展开更多
文摘Objectives To investi-gate the effect of electrical stimulation during absolute refractory period on contraction and relaxation of ventricular myocytes from normal guinea - pigs. Methods The guinea - pig ventricular myocytes were obtained by enzyme digesting, and the extent of its contraction and relaxation and Ca2+ transient were recorded through the motion edge detection system. Results (1) The contracting extent of guinea - pig ventricular myocytes increased 15.45±6.48% , and the peak velocity of shorting (contraction) and the peak veloci ty of relengthening (relaxation) increased 15.97 ± 8.37% and 21. 63±8. 06% respectively (n = 10); (2) The extent of ventricular myocytes fluorescence ra tio(360/380) (F360/F380) increased 22. 55±9. 08%, and the peak velocity of ventricular myocytes F360 /F380 increased 36. 75 ± 9. 77% and 23. 62±4. 47% during shorting and relengthening respectively (n = 6) . Conclusions Appropriate electrical stimulation may strengthen the contracting and relaxing function of normal guinea - pig ventricular myocytes.
