Two New C-glucoside Flavonoids from Leaves of Crataegus pinnatifida Bge.var.major N.E.Br.

Two new C-glucoside flavonoids, namely 8-C-b-D-(2-O-acetyl) glucofuranosyl apigenin and 3-O-acetylvitexin, were isolated from leaves of Crataegus pinnatifida Bge. var. major N. E. Br.. Their structures were elucidated by the spectroscopic means and chemical evidence.

山楂叶中新黄酮化合物的分离与结构鉴定

Two new flavonoids,namely pinnatifida A (1) and pinnatifida B (2),along with four known compounds,vitexin(3),6″ O acetyl vitexin(4),2″ O acetyl vitexin (5) and 2″ O rhamnosyl vitexin (6),were isolated from the leaves of Crataegus pinnatifida Bge.var. major N.E.Br.Their structures were elucidated by the spectroscopic analysis and chemical evidence.Compound (4) and (5) were first isolated from the leaves of Crataegus pinnatifida Beg.var. major N.E.Br.

健脾和胃颗粒质量标准的研究

目的 研究并建立健脾和胃颗粒的质量标准。方法 采用TLC法对健脾和胃颗粒中的丹参、山楂和党参三味药进行定性鉴别;采用HPLC法测定健脾和胃颗粒丹参中的丹酚酸B含量,Agilent Eclipse Plus C_（18）柱（4.6×250 mm,5μm）,以甲醇-乙腈-1.67%甲酸水溶液（27∶9∶64）为流动相;检测波长为286 nm,柱温25℃,流速1.0 m L/min,进样量10μL。结果 试验采用TLC定性鉴别的三味药色谱斑点清晰,阴性对照无干扰;以HPLC法测定健脾和胃颗粒丹参中的丹酚酸B,丹酚酸B浓度在12.1856~389.94μg/m L范围内与峰面积呈良好的线性关系,线性回归方程为Y=6.5459X-3.7811（r=0.9999,n=6）,回收率为99.97%,RSD为1.07%。结论 本试验确定的方法操作简单、灵敏、准确,可用于健脾和胃颗粒的质量控制。