Two rice genotypes with different drought tolerance, namely Jin 23B (drought tolerant) and Zhenshan 97B (drought sensitive), were used to study the antioxidant enzyme activities, soluble sugar and starch contents ...Two rice genotypes with different drought tolerance, namely Jin 23B (drought tolerant) and Zhenshan 97B (drought sensitive), were used to study the antioxidant enzyme activities, soluble sugar and starch contents in spikelets, pistil water potential and pollen number on a stigma under water-deficit stress at the flowering stage, which were involved in the spikelet sterility. Compared with respective controls, drought stress induced more serious decreases of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities and more significant increase of malonaldehyde (MDA) content in spikekets of Zhenshan 97B than in Jin 23B on 9 and 12 days after water stress (DAWS). The soluble sugar and starch contents increased significantly in spikelets of Jin 23B, but decreased significantly in spikelets of Zhenshan 97B during 9-12 DAWS. The pistil maintained higher water potential in Jin 23B than in Zhenshan 97B during 3-6 DAWS and 9-12 DAWS. In addition, water stress induced more significant decrease in the pollen number on a stigma as well as the percentage of unfilled grains in Zhenshan 97B than in Jin 23B. Thus, it is suggested that water stress induced spikelet sterility by damaging antioxidant enzyme activities, reducing carbohydrate content in spikelets and decreasing pistil water potential at the flowering stage in rice.展开更多
Brassinosteroids(BRs)play critical roles in a wide range of plant developmental processes.However,it is unknown whether and how BRs mediate the effect of high temperature(HT)stress during anthesis on the pistil activi...Brassinosteroids(BRs)play critical roles in a wide range of plant developmental processes.However,it is unknown whether and how BRs mediate the effect of high temperature(HT)stress during anthesis on the pistil activity of photo-thermosensitive genetic male-sterile(PTSGMS)rice(Oryza sativa L.)lines.This study investigated the question.Three pot-grown PTSGMS rice lines were subjected to HT stress during anthesis.The contents of 24-epibrassinolide(24-EBL)and 28-homobrassinolide(28-HBL),the major forms of BR in rice plants,and levels of reactive oxygen species(ROS)or antioxidants(AOS),hydrogen peroxide(H2O2),1-aminocylopropane-1-carboxylic acid(ACC),ascorbic acid(AsA),and catalase activity in pistils,were determined.HT stress significantly reduced the contents of both 24-EBL and 28-EBL relative to those under normal temperatures,but the reduction varied by PTSGMS line.A line with higher BR contents under HT stress showed lower contents of ACC and H2O2,higher catalase activity and AsA content in pistils,and higher fertilization rate,seed-setting rate,and seed yield when the line was crossed with a restorer line,indicating that higher levels of BRs increase HT stress resistance.Applying 24-EBL,28-HBL or an inhibitor of BR biosynthesis confirmed the roles of BRs in response to HT stress.The results suggest that BRs mediate the effect of HT stress on pistil activity during anthesis and alleviate the harm of HT stress by increasing AOS and suppressing ROS generation.展开更多
Plant height and fertility are two important traits of wheat (Triticum aestivum L.), whose mutants are ideal materials for studies on molecular mechanisms of stem and lforal organ development. In this study, we identi...Plant height and fertility are two important traits of wheat (Triticum aestivum L.), whose mutants are ideal materials for studies on molecular mechanisms of stem and lforal organ development. In this study, we identiifed a dwarf, multi-pistil and male sterile (dms hereafter) wheat mutant from Zhoumai 18. Simple sequence repeat (SSR) marker assay with 181 primer pairs showed that only one locus of GWM148-2B was divergent between Zhoumai 18 and dms. There were three typical phenotypes in the progeny of dms, tal (T;ca. 0.8 m), semi-dwarf (M;ca. 0.6 m) and dwarf (D;under 0.3 m) plants. Morphological investigation indicated that the internode length of M was shortened by about 20–50 mm each;the internode number of D was 2 less than that of T and Zhoumai 18, and its internode length was shorter also. The pol en vigor and hybridization test demonstrated that dms mutant was male sterility. Segregated phenotypes in progeny of M suggested that the multi-pistils and sterility were control ed by one recessive gene locus which was designated as dms temporarily, and the plant height was control ed by a semi-dominant gene locus Dms. Therefore, progeny individuals of the dms had three genotypes, DmsDms for tal plants, Dmsdms for semi-dwarf plants and dmsdms for dwarf plants. The mutant progenies were individual y selected and propagated for more than 6 generations, thus a set of near isogenic lines of T, M and D for dms were developed. This study provides a set germplasms for studies on molecular mechanisms of wheat stem and spike development.展开更多
We identified a wheat(Triticum aestivum L.) multi-pistil mutant from an F_2 breeding population in 2012, named 12 TP(three pistils in one floret). Genetic analysis showed that one dominant gene locus controlled the mu...We identified a wheat(Triticum aestivum L.) multi-pistil mutant from an F_2 breeding population in 2012, named 12 TP(three pistils in one floret). Genetic analysis showed that one dominant gene locus controlled the multi-pistil trait. Using homozygous normal and multi-pistil lines(near-isogenic lines; NILs) derived from the original mutant 12 TP, a simple sequence repeat(SSR) marker assay located the 12 TP locus on chromosome arm 2 DL. Four SSR markers were linked to 12 TP and their order was Xcfd233→Xcfd62-12 TP→Xwmc41→Xcfd168 at 15.85, 10.47, 2.89, and 10.37 cM, respectively. The average genetic expressivity of the trait ‘three pistils in one floret' was more than 98% in seven homozygous 12 TP lines; however, the average genetic expressivity in heterozygous F_1 plants was about 49%. Thus, the 12 TP is a semi-dominant gene locus, which differ from all previously reported multi-pistil mutants. Mutant 12 TP is a new useful germplasm for study of wheat floral development and for breeding of high yield wheat.展开更多
In order to excavate the function genes of stage-specific proteins in the development process of mulberry pistillate flowers, using fruit mulberry cuhivar ' Da 10' as experiment material, two-dimensional electrophor...In order to excavate the function genes of stage-specific proteins in the development process of mulberry pistillate flowers, using fruit mulberry cuhivar ' Da 10' as experiment material, two-dimensional electrophoresis and mass-spectrometric technology were used to investigate specifically expressed proteins of mul- berry pistillate flowers in different flowering periods. The results showed that 471 ± 4,450 ± 15 and 446 ± 14 protein spots were determined in mulberry pistil- late flowers at full-bloom stage, initial flowering stage and terminal flowering stage respectively, including nine protein spots with characteristics of stage-specific ex- pression and clear electrophoretic bands.. By mass spectrometry analysis, database retrieval and bioinformatics analysis, five components were successfully identi- fied as lactoylglutathione lyase-like protein, perchloric acid soluble translation inhibitor protein, ubiquitin-conjugating enzyme 1 like protein, putative ethylene re- sponse protein and 3-hydroxyisobutyrate dehychngenase, which were involved in stress resistance reaction, protein catabolism, signal transduction, glycometabolism and other biological processes in plants, which indicated that these proteins might play an important biological function in the normal development and pollination fertilization of mulberry pistillate flowers.展开更多
Allium is a complicated genus that includes approximately 1000 species.Although its morphology is well studied,the taxonomic importance of many morphological traits,including floral traits,are poorly understood.Here,w...Allium is a complicated genus that includes approximately 1000 species.Although its morphology is well studied,the taxonomic importance of many morphological traits,including floral traits,are poorly understood.Here,we examined and measured the floral characteristics of 87 accessions of 74 Allium taxa(belonging to 30 sections and nine subgenera)from Central to Eastern Asian countries.We then examined the taxonomic relationships between select flower characteristics and a phylogenetic tree based on ITS sequences.Our results confirm that floral morphology provides key taxonomic information to assess species delimitation in Allium.We found that perianth color is an important characteristic within the subg.Melanocrommyum,Polyprason,and Reticulatobulbosa.In subg.Allium,Cepa,and Rhizirideum,significant characteristics include ovary shape,perianth shape,and inner tepal apex.For species in subg.Angunium,the key taxonomic character is ovule number(only one ovule in per locule).In the subg.Allium,Cepa,Polyprason,and Reticulatobulbosa,which belong to the third evolutionary line of Allium,hood-like appendages occur in the ovary,although these do not occur in subg.Rhizirideum.Our results also indicated that the flower morphology of several species in some sections are not clearly distinguished,e.g.,sect.Sacculiferum(subg.Cepa)and sect.Tenuissima(subg.Rhizirideum).This study provides detailed photographs and descriptions of floral characteristics and information on general distributions,habitats,and phenology of the studied taxa.展开更多
Floral morphogenesis and the development of Cercidiphyllumjaponicum Sieb. et Zucc. were observed by scanning electron microscopy (SEM). The results showed that the pistillate inflorescences were congested spikes wit...Floral morphogenesis and the development of Cercidiphyllumjaponicum Sieb. et Zucc. were observed by scanning electron microscopy (SEM). The results showed that the pistillate inflorescences were congested spikes with the flowers arranged opposite. Great differences between the so-called "bract" and the vegetative leaf were observed both in morphogenesis and morphology. In morphogenesis, the "bract" primordium is crescent-shaped, truncated at the apex and not conduplicate, has no stipule primordium at the base but does have some inconspicuous teeth in the margin that are not glandular. The leaf primordium is triangular, cycloidal at the apex, conduplicate, has two stipule primordia at the base, has one gland-tooth at the apex occurring at first and some gland-teeth in the margin that occur later. In morphology, the "bract" is also different to the vegetative leaf in some characteristics that were also illustrated in the present paper. Based on the hypothesis that the bract is more similar to the vegetative leaf than the tepal, we considered that the so-called "bract" of C.japonicum might be the tepal of the pistillate flower in morphological nature. Therefore, each pistillate flower contains a tepal and a carpel. We did not find any trace of other floral organs in the morphogenesis of the pistillate flower. Therefore we considered that the unicarpellate status of extant Cercidiphyllum might be to highly reduce and advance characteristics that make the extant Cercidiphyllum isolated from both fossil Cercidiphyllum-like plants and its extant affinities.展开更多
In order to investigate the cause of poor fruit set in 'Zuili' plums,anatomical examinations of post-bloom pistils were conducted and the dates of young fruit drop were recorded during the growing seasons of 2...In order to investigate the cause of poor fruit set in 'Zuili' plums,anatomical examinations of post-bloom pistils were conducted and the dates of young fruit drop were recorded during the growing seasons of 2008 and 2009.Pistils of cv.'Black Amber' were also examined as an abundant setting control.Two major dropping periods were detected in 'Zuili':one during the first 5 d after full bloom (DAF) and another between 10 and 17 DAF.Anatomical analyses of the pistils at the full bloom stage revealed that half of the ovules had not developed embryos,which may have caused their early drop.In most dropped pistils collected at 17 DAF,the micropyle had not been penetrated by a pollen tube,indicating that they were not fertilized.'Zuili' ovules initiated embryo division at 10-12 DAF,although thereafter embryo development was retarded when compared to the rates observed in 'Black Amber'.Ovule fertilization failure and inactive embryo development after ovule fertilization may be the major causes of the later fruit drop observed in 'Zuili' plum trees.展开更多
基金supported by the National Natural Science Foundation of China(GrantNo.30871473)the project from the China National Rice Research Institute(Grant No.2009RG003-5)the projects from local government of Zhejiang Province,China(Grant Nos.2008C22073,2009C32048 and SN200806)
文摘Two rice genotypes with different drought tolerance, namely Jin 23B (drought tolerant) and Zhenshan 97B (drought sensitive), were used to study the antioxidant enzyme activities, soluble sugar and starch contents in spikelets, pistil water potential and pollen number on a stigma under water-deficit stress at the flowering stage, which were involved in the spikelet sterility. Compared with respective controls, drought stress induced more serious decreases of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities and more significant increase of malonaldehyde (MDA) content in spikekets of Zhenshan 97B than in Jin 23B on 9 and 12 days after water stress (DAWS). The soluble sugar and starch contents increased significantly in spikelets of Jin 23B, but decreased significantly in spikelets of Zhenshan 97B during 9-12 DAWS. The pistil maintained higher water potential in Jin 23B than in Zhenshan 97B during 3-6 DAWS and 9-12 DAWS. In addition, water stress induced more significant decrease in the pollen number on a stigma as well as the percentage of unfilled grains in Zhenshan 97B than in Jin 23B. Thus, it is suggested that water stress induced spikelet sterility by damaging antioxidant enzyme activities, reducing carbohydrate content in spikelets and decreasing pistil water potential at the flowering stage in rice.
基金supported by the National Natural Science Foundation of China(31771710,31901445)the National Key Research and Development Program of China(2016YFD03002064,2018YFD0300800)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the Top Talent Supporting Program of Yangzhou University(2015-01)。
文摘Brassinosteroids(BRs)play critical roles in a wide range of plant developmental processes.However,it is unknown whether and how BRs mediate the effect of high temperature(HT)stress during anthesis on the pistil activity of photo-thermosensitive genetic male-sterile(PTSGMS)rice(Oryza sativa L.)lines.This study investigated the question.Three pot-grown PTSGMS rice lines were subjected to HT stress during anthesis.The contents of 24-epibrassinolide(24-EBL)and 28-homobrassinolide(28-HBL),the major forms of BR in rice plants,and levels of reactive oxygen species(ROS)or antioxidants(AOS),hydrogen peroxide(H2O2),1-aminocylopropane-1-carboxylic acid(ACC),ascorbic acid(AsA),and catalase activity in pistils,were determined.HT stress significantly reduced the contents of both 24-EBL and 28-EBL relative to those under normal temperatures,but the reduction varied by PTSGMS line.A line with higher BR contents under HT stress showed lower contents of ACC and H2O2,higher catalase activity and AsA content in pistils,and higher fertilization rate,seed-setting rate,and seed yield when the line was crossed with a restorer line,indicating that higher levels of BRs increase HT stress resistance.Applying 24-EBL,28-HBL or an inhibitor of BR biosynthesis confirmed the roles of BRs in response to HT stress.The results suggest that BRs mediate the effect of HT stress on pistil activity during anthesis and alleviate the harm of HT stress by increasing AOS and suppressing ROS generation.
基金supported by the National 863 Program of China (2012AA101105)the Henan Key Scientific and Technological Project, China (122101110200)
文摘Plant height and fertility are two important traits of wheat (Triticum aestivum L.), whose mutants are ideal materials for studies on molecular mechanisms of stem and lforal organ development. In this study, we identiifed a dwarf, multi-pistil and male sterile (dms hereafter) wheat mutant from Zhoumai 18. Simple sequence repeat (SSR) marker assay with 181 primer pairs showed that only one locus of GWM148-2B was divergent between Zhoumai 18 and dms. There were three typical phenotypes in the progeny of dms, tal (T;ca. 0.8 m), semi-dwarf (M;ca. 0.6 m) and dwarf (D;under 0.3 m) plants. Morphological investigation indicated that the internode length of M was shortened by about 20–50 mm each;the internode number of D was 2 less than that of T and Zhoumai 18, and its internode length was shorter also. The pol en vigor and hybridization test demonstrated that dms mutant was male sterility. Segregated phenotypes in progeny of M suggested that the multi-pistils and sterility were control ed by one recessive gene locus which was designated as dms temporarily, and the plant height was control ed by a semi-dominant gene locus Dms. Therefore, progeny individuals of the dms had three genotypes, DmsDms for tal plants, Dmsdms for semi-dwarf plants and dmsdms for dwarf plants. The mutant progenies were individual y selected and propagated for more than 6 generations, thus a set of near isogenic lines of T, M and D for dms were developed. This study provides a set germplasms for studies on molecular mechanisms of wheat stem and spike development.
基金supported by the National Science Fund of China (31571646)the Special Fund for Key Agricultural Project in Henan Province, China in 2016 (161100110400)
文摘We identified a wheat(Triticum aestivum L.) multi-pistil mutant from an F_2 breeding population in 2012, named 12 TP(three pistils in one floret). Genetic analysis showed that one dominant gene locus controlled the multi-pistil trait. Using homozygous normal and multi-pistil lines(near-isogenic lines; NILs) derived from the original mutant 12 TP, a simple sequence repeat(SSR) marker assay located the 12 TP locus on chromosome arm 2 DL. Four SSR markers were linked to 12 TP and their order was Xcfd233→Xcfd62-12 TP→Xwmc41→Xcfd168 at 15.85, 10.47, 2.89, and 10.37 cM, respectively. The average genetic expressivity of the trait ‘three pistils in one floret' was more than 98% in seven homozygous 12 TP lines; however, the average genetic expressivity in heterozygous F_1 plants was about 49%. Thus, the 12 TP is a semi-dominant gene locus, which differ from all previously reported multi-pistil mutants. Mutant 12 TP is a new useful germplasm for study of wheat floral development and for breeding of high yield wheat.
基金Supported by National Natural Science Foundation of China(31072087)
文摘In order to excavate the function genes of stage-specific proteins in the development process of mulberry pistillate flowers, using fruit mulberry cuhivar ' Da 10' as experiment material, two-dimensional electrophoresis and mass-spectrometric technology were used to investigate specifically expressed proteins of mul- berry pistillate flowers in different flowering periods. The results showed that 471 ± 4,450 ± 15 and 446 ± 14 protein spots were determined in mulberry pistil- late flowers at full-bloom stage, initial flowering stage and terminal flowering stage respectively, including nine protein spots with characteristics of stage-specific ex- pression and clear electrophoretic bands.. By mass spectrometry analysis, database retrieval and bioinformatics analysis, five components were successfully identi- fied as lactoylglutathione lyase-like protein, perchloric acid soluble translation inhibitor protein, ubiquitin-conjugating enzyme 1 like protein, putative ethylene re- sponse protein and 3-hydroxyisobutyrate dehychngenase, which were involved in stress resistance reaction, protein catabolism, signal transduction, glycometabolism and other biological processes in plants, which indicated that these proteins might play an important biological function in the normal development and pollination fertilization of mulberry pistillate flowers.
基金supported by research grants from the Korea National Arboretum (Grant No. KNA1-1-26, 20-1)the Mid-level professor Financial Program at Changwon National University in 2023
文摘Allium is a complicated genus that includes approximately 1000 species.Although its morphology is well studied,the taxonomic importance of many morphological traits,including floral traits,are poorly understood.Here,we examined and measured the floral characteristics of 87 accessions of 74 Allium taxa(belonging to 30 sections and nine subgenera)from Central to Eastern Asian countries.We then examined the taxonomic relationships between select flower characteristics and a phylogenetic tree based on ITS sequences.Our results confirm that floral morphology provides key taxonomic information to assess species delimitation in Allium.We found that perianth color is an important characteristic within the subg.Melanocrommyum,Polyprason,and Reticulatobulbosa.In subg.Allium,Cepa,and Rhizirideum,significant characteristics include ovary shape,perianth shape,and inner tepal apex.For species in subg.Angunium,the key taxonomic character is ovule number(only one ovule in per locule).In the subg.Allium,Cepa,Polyprason,and Reticulatobulbosa,which belong to the third evolutionary line of Allium,hood-like appendages occur in the ovary,although these do not occur in subg.Rhizirideum.Our results also indicated that the flower morphology of several species in some sections are not clearly distinguished,e.g.,sect.Sacculiferum(subg.Cepa)and sect.Tenuissima(subg.Rhizirideum).This study provides detailed photographs and descriptions of floral characteristics and information on general distributions,habitats,and phenology of the studied taxa.
基金the National Natural Science Foundation of China(30640013).
文摘Floral morphogenesis and the development of Cercidiphyllumjaponicum Sieb. et Zucc. were observed by scanning electron microscopy (SEM). The results showed that the pistillate inflorescences were congested spikes with the flowers arranged opposite. Great differences between the so-called "bract" and the vegetative leaf were observed both in morphogenesis and morphology. In morphogenesis, the "bract" primordium is crescent-shaped, truncated at the apex and not conduplicate, has no stipule primordium at the base but does have some inconspicuous teeth in the margin that are not glandular. The leaf primordium is triangular, cycloidal at the apex, conduplicate, has two stipule primordia at the base, has one gland-tooth at the apex occurring at first and some gland-teeth in the margin that occur later. In morphology, the "bract" is also different to the vegetative leaf in some characteristics that were also illustrated in the present paper. Based on the hypothesis that the bract is more similar to the vegetative leaf than the tepal, we considered that the so-called "bract" of C.japonicum might be the tepal of the pistillate flower in morphological nature. Therefore, each pistillate flower contains a tepal and a carpel. We did not find any trace of other floral organs in the morphogenesis of the pistillate flower. Therefore we considered that the unicarpellate status of extant Cercidiphyllum might be to highly reduce and advance characteristics that make the extant Cercidiphyllum isolated from both fossil Cercidiphyllum-like plants and its extant affinities.
基金Project(No.20031007)supported by the Jiaxing Municipal Science and Technology Projects,Zhejiang Province,China
文摘In order to investigate the cause of poor fruit set in 'Zuili' plums,anatomical examinations of post-bloom pistils were conducted and the dates of young fruit drop were recorded during the growing seasons of 2008 and 2009.Pistils of cv.'Black Amber' were also examined as an abundant setting control.Two major dropping periods were detected in 'Zuili':one during the first 5 d after full bloom (DAF) and another between 10 and 17 DAF.Anatomical analyses of the pistils at the full bloom stage revealed that half of the ovules had not developed embryos,which may have caused their early drop.In most dropped pistils collected at 17 DAF,the micropyle had not been penetrated by a pollen tube,indicating that they were not fertilized.'Zuili' ovules initiated embryo division at 10-12 DAF,although thereafter embryo development was retarded when compared to the rates observed in 'Black Amber'.Ovule fertilization failure and inactive embryo development after ovule fertilization may be the major causes of the later fruit drop observed in 'Zuili' plum trees.