Efficacy of Yiqi Fumai (Freeze-Dried Powder) on Ischemic Diastolic Heart Failure

Objective: To evaluate the efficacy of Yiqi Fumai freeze-dry powder (YFP) on ischemic diastolic heart failure. Methods: 100 patients diagnosed with unstable angina accompanying ischemic diastolic heart failure (IDHF) were selected randomly. 52 patients with TCM syndrome of qi-yin deficiency were divided into Chinese and Western combination therapy group. 48 patients have no TCM syndrome of qi-yin deficiency, and were treated with standard western medicine. After treatment, Seattle Angina Questionnaire Evaluation, ECG, conventional and stress echocardiography (SE) index, NT-proBNP were compared between before and after treatment. Results: The differences of PL, TS, DP between before and after treatment by YFP were statistically significant

Component Analysis of Shuanghuanglian Freeze-dried Powder and Its Effects on Human Hepatocyte Function when Combined with Cefradine

[Objectives]To detect the contents of components in Shuanghuanglian freeze-dried powder and to explore the effects of Shuanghuanglian freeze-dried powder and its components on human hepatocytes(HL-7702)alone or in combination with cefradine.[Methods]High performance liquid chromatography(HPLC)was used to detect the contents of baicalin,wogonin,chlorogenic acid and forsythin,the main components of Shuanghuanglian freeze-dried powder.HL-7702 cells were cultured with Shuanghuanglian freeze-dried powder and the main components of Shuanghuanglian freeze-dried powder alone or in combination with cefradine.Enzyme linked immunosorbent assay(ELISA)was used to detect the contents of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in the cell supernatant after culture,and HPLC was used to detect the expression level of adenosine diphosphate(ADP)and adenosine triphosphate(ATP);agarose gel electrophoresis was used to detect the expression of cyclooxygenase 2(COX-2)and heme oxygenase-1(HO-1)in HL-7702 cells.[Results]In Shuanghuanglian freeze-dried powder for injection,the content of baicalin was the highest,and the content of wogonin was the lowest.Compared with the control group,the expressions of AST and ALT in human hepatocytes(HL-7702)in high-dose baicalin group,forsythin group and wogonin group decreased(P<0.05,P<0.01),while the expression of ALT in chlorogenic acid+cefradine group(0.046 mg/mL)and forsythin+cefradine group(0.046 mg/mL)increased(P<0.05,P<0.01),and the expression of AST had no significant difference(P>0.05);the results in the low-dose group were similar to those in the high-dose group.Compared with the control group,ATP expression in chlorogenic acid group,chlorogenic acid+cefradine group(0.046 mg/mL)and forsythin+cefradine group(0.046 mg/mL)in the high-dose group decreased(P<0.05,P<0.01),and ADP expression was not significantly different(P>0.05);in the low-dose group,the expression of ATP and ADP increased in baicalin group(P<0.05),but decreased in wogonin group,baicalin+cefradine group(0.046 mg/mL)and wogonin group+cefradine group(0.046 mg/mL)(P<0.05,P<0.01).Compared with the control group,the expressions of COX-2 and HO-1 in HL-7702 cells in the cefradine group showed no significant difference(P>0.05).The expression of HO-1 and COX-2 in the different dose groups of Shuanghuanglian and the group combined with cefradine increased,and the difference was significant(P<0.05,P<0.01).[Conclusions]The components of Shuanghuanglian freeze-dried powder for injection had effects on hepatocytes,of which baicalin had a significant effect,and the effect of cefradine on hepatocytes was increased when used in combination with cefradine.

Effect of Yizhikang Powder on Hemorheological Indexes in Dairy Cattle with Retained Placenta

[ Objective ] The aim of the research was to reveal the mechanism of Yizhikang powder treatment on dairy cattle with retained placenta from the hemorheological perspective. [ Method] Dairy cattle with retained placenta were treated with oral administration of Yizhikang powder. And their hemorheological indexes were measured and compared with the corresponding indicators of pre-administration group, healthy group, and control group （sick but untreated）.[ Result] There was large decrease amplitude in the indexes （whole blood viscosity, plasma viscosity, whole blood viscosity reduction viscosity, ESR, ESR equation K value, fibrinogen content, platelet aggregation rate, RBC deformability IF value） of treated dairy cattle with retained placenta. By t test, the indexes, except hematocrit, decreased significantly after treatment （P〈0.05） and reached the status of post partum healthy cows. The hemorheological indexes didn＇t change significantly in the control group before and after treatment. [ Conclusion] Yizhikang powder could significantly improve blood flow state and reduced the occurrence of qi stagnation and blood stasis in perinatal period.

THE DETERMINATION OF ESTRADIOL IN SHEEP PLACENTA POWDER BY RADIO-IMMUNOASSAY

The present study is to determine Estradiol in sheep placenta powder by radio immunoassay. The relative standard deviation was 2 3%～9 8%, the average rate of recovery was 102 9%. The sensitivity and specificity of this method were high. This method can be applied to determine estradiol in health protective food.

Preparation technology of ultra-fine powders of Auricularia auricular

Conventional mechanical method and mechanical method combined with vacuum freeze-drying technology were used to make the ultra-fine powders of edible fungus (Auricularia auricular). The content of basic nutrients, amino acid, micro structure and their properties of raw edible fungus and the edible fungus powders obtained with the two methods were analyzed and compared. The granularity size and micro-structure of the pulverized samples were analyzed by SEM and TEM technology. The average granularity size of the edible fungus powder obtained with mechanical method was 1–5 μm, while that obtained with mechanical method combined with vacuum freeze-drying process was 0.5–1 μm. The ultra-fine powders of edible fungus obtained with the two methods had better water recovery capability and quality, and their preserving time was longer than that of raw edible fungus. All the properties of the ultra-fine powders of edible fungus obtained with the vacuum freeze-drying technology were evidently superior to that of the conventional mechanical method. Keywords Auricularia auricula - Edible fungus - Ultra-fine powders - Vacuum freeze-drying CLC number TS205 Document code B Biography: YANG Chun-yu (1975), female, Ph. D. in Engineering Technology Center of Forestry and Wood Workine, Machinery, Northeast Forestry University, Harbin 150040, P. R. China.Responsible editor: Zhu Hong

羊胎盘预防绝经后骨质疏松症的实验研究

目的探讨口服羊胎盘粉能否预防绝经后骨质疏松症。方法建立骨质疏松大鼠模型,将大鼠随机分为假手术组(Sham)、模型组(OVX)、羊胎盘粉低(L)、中(M)、高剂量组(H)和炔雌醇组(EE)。每两周检测一次全身骨密度,1个半月后处死所有大鼠,称量并计算主要脏器系数,检测离体骨密度、血清骨代谢指标,进行Micro-CT扫描,观察骨组织形态变化。结果6周后OVX组大鼠的全身骨密度显著低于Sham组(P<0.01),但EE组未明显下降,羊胎盘三个组均显著高于OVX组(P<0.01)。离体股骨骨密度的变化呈相似趋势;Micro-CT扫描分析发现,羊胎盘三个组中只有M组的各项参数与Sham组和EE组接近,股骨脱钙后的组织病理学切片HE染色显示出与Micro-CT相似的结果;股骨三点弯曲试验发现,M组的最大载荷及强度均显著高于OVX组(P<0.01,P<0.05);血清骨代谢指标中,M组的骨形成指标BAP和PINP显著高于OVX组,骨吸收指标CTX-I和TRACP则显著低于OVX组。实验过程中,各组大鼠体重始终未出现显著性差异,心、肝、脾、肺、肾等主要脏器的外观无明显异常,器官系数除炔雌醇组的子宫外均无显著性差异。结论口服羊胎盘粉[300 mg/(kg?d)]可以通过促进骨形成、抑制骨吸双向作用,共同调节骨密度来预防切除卵巢大鼠发生骨质疏松,且羊胎盘粉没有产生使子宫内膜增厚的副作用。

Technological Study on Instant Date Powder in Natural Ziziphus jujuba through Vacuum Freeze-drying

[Objective] The aim was to get optimized conditions for date powder with good color and taste.[Method] The pectinase enzymolysis and vacuum freeze-drying technology were used in the extract process of date powder.[Result] The production boasts of superior quality were with rich fragrance and uniform particle under certain process conditions.The optimized conditions for pectinase enzymolysis were:the amount of pectinase reached to 0.1% of that of date syrup,enzymolysis temperature and time were 50 ℃ and 50 min respectively,the pH value was 4.0.The optimized conditions for vaccum freeze-drying were:10% altodextrin,three times volumn of water with the thickness of 7 mm.[Conclusion] The optimized conditions were obtained in this study to produce instant date powder from Ziziphus jujuba.

High-concentration dispersions of exfoliated MoS2 sheets stabilized by freeze-dried silk fibroin powder

Liquid-phase exfoliation （LPE） is an attractive method for the scaling-up of exfoliated MoS2 sheets compared to chemical vapor deposition and mechanical cleavage. However, the MoS2 nanosheet yield from LPE is too small for practical applications. We report a facile method for the scaling-up of exfoliated MoS2 nanosheets using freeze-dried silk fibroin powders. Compared to MoS2 dispersion in the absence of silk fibroin powder, sonicated MoS2 dispersions with silk fibroin powder （MoSJSilk dispersion） show noticeably higher exfoliated MoS2 nanosheet yields, with suspended MoS2 concentrations in MoS2/Silk dispersions sonicated for 2 and 5 h of 1.03 and 1.39 mg.mL-1, respectively. The MoS2 concentration in the MoS2/Silk dispersion after centrifugation above 10,000 rpm is more than four times that without the silk fibroin. The size of the dispersed silk fibroin is controlled by the change of centrifugation rate, showing the removal of silk fibroin above tens of micrometers in size after centrifugation at 2,000 rpm. Size-controlled silk fibroin biomolecules combined with MoS2 nanosheets are expected to increase the practical use of such materials in fields related to tissue engineering, biosensors and electrochemical electrodes. Atomic force microscopy and Raman spectroscopy provide the height of the MoS2 nanosheets spin-cast from MoS2/Silk dispersions, showing thicknesses of 3--6 nm. X-ray photoelectron spectroscopy and X-ray diffraction indicate that the outermost surface layer of the hydrophobic MoS2 crystals interact with oxygen-containing functional groups that exist in the hydrophobic part of silk fibroins. The amphiphilic properties of silk fibroin combined with the MoS2 nanosheets stabilize dispersions by enhancing solvent-material interactions. The large quantities of exfoliated MoS2 nanosheets suspended in the as-synthesized dispersions can be utilized for the fabrication of vapor and electrochemical devices requiring high MoS2 nanosheets contents.

胎盘冻干粉颗粒毒理学评价

目的:对胎盘冻干粉颗粒进行毒理学评价。方法:急性毒性实验选用SPF KM级小鼠,雌、雄各半,共20只,24 h内两次经口灌胃给予受试物,观察小鼠14 d的中毒表现及死亡情况。28 d经口毒性实验选SD大鼠,雌、雄各半,共80只,分为对照组及3个实验组。灌胃28 d后采血,进行血常规指标检测及血液生化指标检测。结果:小鼠急性经口毒性LD_(50)>10 g/(kg bw)。28 d经口毒性实验结果表明,实验期间雌、雄性动物生长状况良好,受试动物均未见明显的中毒症状,一般生理体征、行为、大小便、皮毛等均未见异常;体重、血液学指标、血液生化指标与阴性对照组比较均无差异。结论:胎盘冻干粉颗粒具有食用安全性。

衣滞康散对胎衣不下奶牛血液流变学指标的影响

[目的]从血液流变学角度揭示衣滞康散治疗奶牛胎衣不下的作用机理。[方法]采用衣滞康散以口服给药方式对胎衣不下奶牛进行治疗,测定其血液流变学指标,并与给药前、健康组及对照组(患病未治疗)相应指标进行比较。[结果]用衣滞康散治疗奶牛胎衣不下后,奶牛全血比黏度、血浆比黏度、全血还原比黏度、血沉、血沉方程K值、纤维蛋白原含量、血小板聚集率、红细胞变形性IF均较治疗前显著下降(P<0.05),达到产后健康奶牛水平。而对照组处理前后血液流变学指标未见明显变化。[结论]衣滞康散可以明显改善胎衣不下奶牛的血流状态,减缓围产期气滞血淤的发生。

紫河车超细粉体的表征技术研究

目的:对紫河车细粉和超微细粉体进行表征研究。方法:先用中草药粉碎机将紫河车粉碎成细粉,然后用倍力微粉机对细粉进行超微粉碎;用扫描电镜和粒径测定仪表征。结果细粉与超细粉的d10分别为16.05μm、9.37μm,d50分别为96.69μm、50.57μm,d90分别212.8μm、148.21μm,分布宽度分别为2.035、2.745。结论:经过超微粉碎后,粉体外观性状得到改善。

牦牛胎盘抗氧化作用的研究

[目的]研究牦牛胎盘抗氧化的作用,为牦牛胎盘的开发利用提供理论依据。[方法]采集不同海拔高度牦牛的胎盘,将100只小鼠随机分为高海拔组、中海拔组、低海拔组和对照组,连续28 d分别灌服不同海拔牦牛的胎盘粉溶液和生理盐水,然后快速处死小鼠,采集肝、肺、心肌、骨骼肌、脾脏和肾脏,分别测定各组织内T-SOD的活性和MDA的含量。[结果]高、中海拔的肝脏、各海拔的心肌和肾脏中T-SOD的活性与对照组相比差异显著(P<0.05),高海拔的肺脏和高、中海拔的骨骼肌中T-SOD的活性与对照组相比差异极显著(P<0.01);高、中海拔的骨骼肌和各海拔的肾脏中MDA的含量与对照组相比差异显著(P<0.05),高、中海拔的肝和各海拔的肺脏中MDA的含量与对照组相比差异极显著(P<0.01)。[结论]牦牛胎盘可以显著提高组织中T-SOD的活性,降低MDA的含量,具有抗氧化的作用。

紫河车超微粉体的制备与质量研究

目的选择紫河车超微粉的制备工艺,探讨紫河车的质量控制方法。方法采用超微粉碎技术,并对普通粉和超微粉进行薄层鉴别和氨基酸分析对比试验。结果超微粉粒度、色泽、味道、分散程度较好,薄层鉴别和氨基酸分析表明超微粉与普通粉无显著差异。结论紫河车超微粉可用于产舒康颗粒的生产,其质控方法专属性强。

牦牛胎盘对小鼠组织中T-SOD活性的影响

目的:探讨牦牛胎盘对小鼠组织中T-SOD活性的影响。方法:根据采集到胎盘的海拔高度将100只小白鼠随机分为高海拔组、中海拔组、低海拔组和对照组,连续28d分别灌服不同海拔的牦牛胎盘和生理盐水,然后处死小白鼠快速采取肝、肺、心肌、骨骼肌、脾脏和肾脏,测定各组织内T-SOD的活性。结果:高海拔肺脏,高、中海拔组骨骼肌中T-SOD活性显著高于对照组,差异极显著(P<0.01);各海拔组心肌和肾脏,高、中海拔组肝脏中T-SOD活性高于对照组,差异显著(P<0.05)。结论:牦牛胎盘能够提高小鼠肺、骨骼肌、心肌、肾脏和肝脏中T-SOD的活性。

牦牛胎盘粉对亚急性衰老小鼠部分组织中NO含量的影响

[目的]研究牦牛胎盘粉对亚急性衰老小鼠心、肝、脾、肺、肾、骨骼肌中一氧化氮(NO)含量的影响。[方法]将健康小鼠随机分为正常对照组、模型组、阳性对照组、低海拔胎盘组、中海拔胎盘组和高海拔胎盘组,将除正常对照组外的其他5组用D-半乳糖,造亚急性衰老小鼠模型;然后对低、中和高海拔胎盘组的小鼠建模11 d后,每天灌胃牦牛胎盘粉,剂量为40 mg/kg。阳性对照组每天灌服维生素E 100 mg/kg,45 d后观察心、肝、脾、肺、肾组织中一氧化氮(NO)含量。[结果]高原牦牛胎盘粉可明显降低小鼠心、肝、脾、肺、肾组织中一氧化氮(NO)含量(P<0.01)。[结论]高原牦牛胎盘粉有很好的抗衰老作用。

胎盘冻干粉对小鼠延缓衰老作用的研究

目的研究胎盘冻干粉对小鼠延缓衰老的作用。方法将2月龄雌性小鼠随机分为5组:空白组、衰老组、阳性组、胎盘粉低剂量组和高剂量组。除空白组外,其余各组均采用皮卞注射相同剂量D—半乳糖42 d建立小鼠衰老模型,同时各组分别灌胃相应的药物,最后检测小鼠机体内免疫力及生理调控相关指标。结果胎盘粉2个剂量组小鼠的胸腺指数和脾脏指数显著高于衰老组(P<0,05),大脑中的MDA含量显著低于衰老组(P<0.05),外周血中的CD4和(CD8细胞比例、血清中雌性激素(P和E 2)、免疫球蛋白(lgG)以及造血因子(EPO、G—CSF和M-CSF)的含量均显著高于衰老组(P<0.05)。结论胎盘冻干粉能够通过提高机体免疫力、改善生理调控来达到延缓衰老的作用。

Luminescence properties of nitrogen-rich Ca-SiAlON:Eu2+ phosphors prepared by freeze-drying assisted combustion synthesis

Nitrogen-rich Eu2+-doped Ca-α-SiAlON phosphors(Cam/2-xSi12-m-nAlm+nOnN16-n:xEu) were synthesized by a freeze-drying assisted combustion synthesis(CS) route. Fast-synthesized products with high purity and uniform particle morphology were confirmed by X-ray diffraction(XRD) and scanning electron microscopy(SEM). The analysis of lattice parameters by comparison with empirical equations showed that the as-prepared phosphors had low oxygen content. A series of samples were prepared according to the stoichiometry of Cam/2-0.08Si12-mAlmN16:0.08 Eu for further research. The influences of m value on the luminescence properties were investigated in detail. As m increased, a redshift phenomenon was observed in both the excitation and emission spectra. First-principle electronic structure calculations showed that the 3d energy level of Ca played an important role in the occurrence of the redshift phenomenon.

胎盘组织液-瑞邦注射型骨泰修复牙槽骨缺损的实验性研究

目的观察胎盘组织液-瑞邦注射型骨泰(自固化磷酸钙calcium phosphate cement,CPC)复合材料修复犬的人工牙槽骨缺损的效果。方法在犬的上、下颌牙槽骨处建立骨缺损模型,分别植入胎盘组织液-瑞邦注射型骨泰复合材料为实验组、生理盐水-瑞邦注射型骨泰复合材料为阳性对照组、缺损区不加任何物质为阴性空白对照组。观察术后2周和4周病理组织学的改变,并通过病理图文报告管理系统做组织学评价。结果病理组织学所见:实验组可见膜内化骨,形成新骨明显,与周围组织连接良好;阳性对照组,可见部分新骨形成,植入体与周围组织连接欠佳;阴性空白对照组,有纤维结缔组织生成。结论胎盘组织液-瑞邦注射型骨泰复合材料能够积极促进骨缺损的修复。

Physicochemical Properties and Sensory Evaluation of Sponge Cake Supplemented with Hot Air and Freeze Dried Oyster Mushroom (<i>Pleurotus sajor-caju</i>)

The study was aimed to enhance the nutritional quality of sponge cakes with oyster mushroom (Pleurotus sajor-caju). In this study, the physicochemical, functional, and sensory properties of cake supplemented with five different levels of mushroom powder (0 as control, 5%, 7%, 10%, and 12%) were evaluated. The results revealed that both hot air dried (HD) and freeze dried (FD) mushroom powder contained a higher amount of protein, fat, ash and fiber ontent compared with wheat flour used in cake, but the carbohydrate and moisture content showed the reverse trend. The study also found that increasing the level of fortification from 0% to 12% mushroom powder also increased the protein, fat, ash, and fiber content in the cake. FD mushroom powder significantly (p ≤ 0.05) recorded the highest protein (11.72% - 15.16%), fat (20.01% - 21.9%), ash (1.75% - 1.92%), and fiber (1.39% - 1.74%) with increasing the level of supplementation from 5% - 12%. In contrast, carbohydrate content of cake decreased from 34.13% - 31.15% with increasing the level of FD mushroom from 5% - 12%. Also, the volume and weight of the cake with FD mushroom were higher compared to HD and control sample. The results of sensory evaluation indicated that sponge cake with 10% FD oyster mushroom was rated the most acceptable after the control.

高原牦牛胎盘粉对小鼠部分组织中MDA含量的影响

将100只小白鼠随机分为四个组(高海拔组、中海拔组、低海拔组和对照组),连续28d分别灌服不同海拔的牦牛胎盘粉和生理盐水,28d后采取肝、肺、心肌、骨骼肌、脾脏和肾脏,测定各组织内MDA的含量。结果表明牦牛胎盘粉能够降低小鼠肺脏、肝脏、肾脏、骨骼肌中MDA的含量。