Practical application of new methods of а plant genome transformation resulted in the need for strict regulation of the safety assessment process of genetically modified organisms of plant origin, intended for use in...Practical application of new methods of а plant genome transformation resulted in the need for strict regulation of the safety assessment process of genetically modified organisms of plant origin, intended for use in food. The devise of Genetically Modified Organisms (GMO) of plant origin safety assessment system, which is currently valid in the Russian Federation, was initiated in 1995-1996. Not only does the system accumulate all domestic and foreign experience, it also includes the latest scientific approaches, based on achievements of fundamental science. In the present article authors focused on issues of the basic legislative regulations of plant biotechnology in the Russian Federation, and approaches to the safety assessment of GM food and control over such food. GMO safety assessment is carried out at the stage of State registration. The subject of registration is novel food products derived from GMO, manufactured in the Russian Federation, as well as food products, entering the Russian Federation for the first time. In the system of medical and biological assessment of the GMO safety, along with general toxicological research, the study of specific types of toxicity in in vivo experiments takes a prominent place. In accordance with established research practice, which uses an integrated approach, and provides with the most complete and reliable information on potential reprotoxic, genotoxic, immunotoxic and allergenic effects of GMO, as well as enables to reveal possible unintended effects of genetic modification. From 1999 to 2016 22 GM lines passed the entire cycle of medical-biological investigations. By 2016 there had been amassed scientific foundation on GMO safety, which includes the analysis of result of research, carried out within the framework of GMO state registration in the Russian Federation, as well as domestic and international data, presented by scientific literature on the problem of biotechnological food safety, at both registration stage and post-registration monitoring stage. To sum up, in the Russian Federation the most essential issues, allowing to use GMO for food purpose have been resolved: 1) legislation, normative and methodical foundations, regulating safety assessment and control over GMO turnover, as well as ensuring customer awareness about presence of GMO in food products, have been created;2) scientifically proven evidence on the lack of negative effects for human health, i.e. GMO safety has been amassed;3) the possibility of control over GMO turnover throughout the food market of the country has been sustained.展开更多
The evolution of new traits in living organisms occurs via the processes of mutation,recombination,genetic drift,and selection.These processes that have resulted in the immense biol ogical diversity on our planet are ...The evolution of new traits in living organisms occurs via the processes of mutation,recombination,genetic drift,and selection.These processes that have resulted in the immense biol ogical diversity on our planet are also being employed in metabolic engineering to optimize enzymes and pathways,create new-to-nature reactions,and synthesize complex natural products in heterologous systems.In this review,we discuss two evolution-aided strategies for metabolic engineering-directed evolution,which improves upon existing genetic templates using the evolutionary process,and combinatorial pathway reconstruction,which brings together genes evolved in different organisms into a single heterol ogous host.We discuss the general principles of these strategies,describe the technologies involved and the molecular traits they influence,provide examples of their use,and discuss the roadblocks that need to be addressed for their wider adoption.A better understanding of these strategies can provide an impetus to research on gene function discovery and biochemical evolution,which is foundational for improved metabolic engineering.These evolution-aided approaches thus have a substantial potential for improving our understanding of plant metabolism in general,for enhancing the production of plant metabolites,and in sustainable agriculture.展开更多
Betalains are tyrosine-derived red-violet and yellow pigments found exclusively in plants of the Caryophyllales order, which have drawn both scientific and economic interest. Nevertheless, research into betalain chemi...Betalains are tyrosine-derived red-violet and yellow pigments found exclusively in plants of the Caryophyllales order, which have drawn both scientific and economic interest. Nevertheless, research into betalain chemistry, biochemistry, and function has been limited as comparison with other major classes of plant pigments such as anthocyanins and carotenoids. The core biosynthetic pathway of this pigment class has only been fully elucidated in the past few years, opening up the possibility for betalain pigment engineering in plants and microbes. In this review, we discuss betalain metabolism in light of recent advances in the field, with a current survey of characterized genes and enzymes that take part in be- talain biosynthesis, catabolism, and transcriptional regulation, and an outlook of what is yet to be discovered. A broad view of currently used and potential new sources for betalains, including utilization of natural sources or metabolic engineering, is provided together with a summary of potential applications of beta- lains in research and commercial use.展开更多
文摘Practical application of new methods of а plant genome transformation resulted in the need for strict regulation of the safety assessment process of genetically modified organisms of plant origin, intended for use in food. The devise of Genetically Modified Organisms (GMO) of plant origin safety assessment system, which is currently valid in the Russian Federation, was initiated in 1995-1996. Not only does the system accumulate all domestic and foreign experience, it also includes the latest scientific approaches, based on achievements of fundamental science. In the present article authors focused on issues of the basic legislative regulations of plant biotechnology in the Russian Federation, and approaches to the safety assessment of GM food and control over such food. GMO safety assessment is carried out at the stage of State registration. The subject of registration is novel food products derived from GMO, manufactured in the Russian Federation, as well as food products, entering the Russian Federation for the first time. In the system of medical and biological assessment of the GMO safety, along with general toxicological research, the study of specific types of toxicity in in vivo experiments takes a prominent place. In accordance with established research practice, which uses an integrated approach, and provides with the most complete and reliable information on potential reprotoxic, genotoxic, immunotoxic and allergenic effects of GMO, as well as enables to reveal possible unintended effects of genetic modification. From 1999 to 2016 22 GM lines passed the entire cycle of medical-biological investigations. By 2016 there had been amassed scientific foundation on GMO safety, which includes the analysis of result of research, carried out within the framework of GMO state registration in the Russian Federation, as well as domestic and international data, presented by scientific literature on the problem of biotechnological food safety, at both registration stage and post-registration monitoring stage. To sum up, in the Russian Federation the most essential issues, allowing to use GMO for food purpose have been resolved: 1) legislation, normative and methodical foundations, regulating safety assessment and control over GMO turnover, as well as ensuring customer awareness about presence of GMO in food products, have been created;2) scientifically proven evidence on the lack of negative effects for human health, i.e. GMO safety has been amassed;3) the possibility of control over GMO turnover throughout the food market of the country has been sustained.
文摘The evolution of new traits in living organisms occurs via the processes of mutation,recombination,genetic drift,and selection.These processes that have resulted in the immense biol ogical diversity on our planet are also being employed in metabolic engineering to optimize enzymes and pathways,create new-to-nature reactions,and synthesize complex natural products in heterologous systems.In this review,we discuss two evolution-aided strategies for metabolic engineering-directed evolution,which improves upon existing genetic templates using the evolutionary process,and combinatorial pathway reconstruction,which brings together genes evolved in different organisms into a single heterol ogous host.We discuss the general principles of these strategies,describe the technologies involved and the molecular traits they influence,provide examples of their use,and discuss the roadblocks that need to be addressed for their wider adoption.A better understanding of these strategies can provide an impetus to research on gene function discovery and biochemical evolution,which is foundational for improved metabolic engineering.These evolution-aided approaches thus have a substantial potential for improving our understanding of plant metabolism in general,for enhancing the production of plant metabolites,and in sustainable agriculture.
文摘Betalains are tyrosine-derived red-violet and yellow pigments found exclusively in plants of the Caryophyllales order, which have drawn both scientific and economic interest. Nevertheless, research into betalain chemistry, biochemistry, and function has been limited as comparison with other major classes of plant pigments such as anthocyanins and carotenoids. The core biosynthetic pathway of this pigment class has only been fully elucidated in the past few years, opening up the possibility for betalain pigment engineering in plants and microbes. In this review, we discuss betalain metabolism in light of recent advances in the field, with a current survey of characterized genes and enzymes that take part in be- talain biosynthesis, catabolism, and transcriptional regulation, and an outlook of what is yet to be discovered. A broad view of currently used and potential new sources for betalains, including utilization of natural sources or metabolic engineering, is provided together with a summary of potential applications of beta- lains in research and commercial use.
