OBJECTIVE：To compare and elucidate the antioxidant effi cacy of ethanolic and hydroethanolic extracts of Indigofera tinctoria Linn.（Fabaceae family）.METHODS：Various in-vitro antioxidant assays and free radical-sca...OBJECTIVE：To compare and elucidate the antioxidant effi cacy of ethanolic and hydroethanolic extracts of Indigofera tinctoria Linn.（Fabaceae family）.METHODS：Various in-vitro antioxidant assays and free radical-scavenging assays were done.Quantitative measurements of various phytoconstituents,reductive abilities and chelating potential were carried out along with standard compounds.Half inhibitory concentration（IC50）values for ethanol and hydroethanol extracts were analyzed and compared with respective standards.RESULTS：Hydroethanolic extracts showed considerably more potent antioxidant activity in comparison to ethanol extracts.Hydroethanolic extracts had lower IC50values than ethanol extracts in the case of DPPH,metal chelation and hydroxyl radical-scavenging capacity（829,659 and 26.7μg/m L）but had slightly higher values than ethanol in case of SO2-and NO2-scavenging activity（P〈0.001 vs standard）.Quantitative measurements also showed that the abundance of phenolic and fl avonoid bioactive phytoconstituents were signifi cantly（P〈0.001）greater in hydroethanol extracts（212.920 and 149.770 mg GAE and rutin/g of plant extract respectively）than in ethanol extracts（211.691 and 132.603 mg GAE and rutin/g of plant extract respectively）.K arl Pearson’s co rrelation analysis（r2）between various antioxidant parameters and bioactive components also associated the antioxidant potential of I.tinctoria with various phytoconstituents,especially phenolics,fl avonoids,saponins and tannins.CONCLUSION：This study may be helpful to draw the attention of researchers towards the hydroethanol extracts of I.tinctoria,which has a high yield,and great prospects in herbal industries to produce inexpensive and powerful herbal products.展开更多
基金technical and laboratory staff of Department of Bioscience and Biotechnology to give support to complete the study
文摘OBJECTIVE：To compare and elucidate the antioxidant effi cacy of ethanolic and hydroethanolic extracts of Indigofera tinctoria Linn.（Fabaceae family）.METHODS：Various in-vitro antioxidant assays and free radical-scavenging assays were done.Quantitative measurements of various phytoconstituents,reductive abilities and chelating potential were carried out along with standard compounds.Half inhibitory concentration（IC50）values for ethanol and hydroethanol extracts were analyzed and compared with respective standards.RESULTS：Hydroethanolic extracts showed considerably more potent antioxidant activity in comparison to ethanol extracts.Hydroethanolic extracts had lower IC50values than ethanol extracts in the case of DPPH,metal chelation and hydroxyl radical-scavenging capacity（829,659 and 26.7μg/m L）but had slightly higher values than ethanol in case of SO2-and NO2-scavenging activity（P〈0.001 vs standard）.Quantitative measurements also showed that the abundance of phenolic and fl avonoid bioactive phytoconstituents were signifi cantly（P〈0.001）greater in hydroethanol extracts（212.920 and 149.770 mg GAE and rutin/g of plant extract respectively）than in ethanol extracts（211.691 and 132.603 mg GAE and rutin/g of plant extract respectively）.K arl Pearson’s co rrelation analysis（r2）between various antioxidant parameters and bioactive components also associated the antioxidant potential of I.tinctoria with various phytoconstituents,especially phenolics,fl avonoids,saponins and tannins.CONCLUSION：This study may be helpful to draw the attention of researchers towards the hydroethanol extracts of I.tinctoria,which has a high yield,and great prospects in herbal industries to produce inexpensive and powerful herbal products.
