Synthesis of Fluorescent Carbon Quantum Dots and Their Application in the Plant Cell Imaging

Carbon quantum dots(CQDs) exhibit tremendous advantages for plant growth study due to its strong fluorescence and good biocompatibility. The fluorescent CQDs were synthesized by the onestep microwave method with the raw materials of citric acid(CA) and urea(UR), and expressed a unique green fluorescence with the optimal excitation wavelength of over 400 nm through adjusting the doping of N elements. It is demonstrated that CQDs can act as deliver media in plant and fluorescent probes for plant cell imaging through directly cultivated in the seedlings of melon and wheat, respectively. Based on the effects of the fluorescent CQDs on plants growth, we can further study the mechanisms of the ions transport in plants.

Study on Five-sense Landscape of Chu Ci Plants Based on NVivo

Chu Ci plants carry profound cultural connotation,and entrust with the poets’ rich emotional understanding and sensory experience.The plant-related sentences in Chu Ci were extracted by NVivo software,and the verses were analyzed and compared based on the five-sense theory.The five-sense images of plants in Chu Ci were extracted from the five aspects of vision,hearing,smell,touch and taste,and the five-sense landscape characteristics of Chu Ci plants were analyzed.The results showed that Chu Ci plants had rich five-sense images,profound artistic conception,magnificent momentum and bold imagination,and had good application potential in landscape architecture.This paper provides a reference for the landscape application of five-sense landscape of Chu Ci plants and a new idea for the inheritance and development of Chu Ci culture.

Image based leaf segmentation and counting in rosette plants

This paper proposes an efficient method to extract the leaf region and count the number of leaves in digital plant images.The plant image analysis plays a significant role in viable and productive agriculture.It is used to record the plant growth,plant yield,chlorophyll fluorescence,plant width and tallness,leaf area,etc.frequently and accurately.Plant growth is a major character to be analyzed among these plant characters and it directly depends on the number of leaves in the plants.In this paper,a new method is presented for leaf region extraction from plant images and counting the number of leaves.The proposed method has three steps.The first step involves a new statistical based technique for image enhancement.The second step involves in the extraction of leaf region in plant image using a graph based method.The third step involves in counting the number of leaves in the plant image by applying Circular Hough Transform(CHT).The proposed work has been experimented on benchmark datasets of Leaf Segmentation Challenge(LSC).The proposed method achieves the segmentation accuracy of 95.4%and it also achieves the counting accuracy of
0.7(DiC)and 2.3(|DiC|)for datasets(A1,A2 and A3),which are better than the state-of-the-art methods.

A Hg(Ⅱ)-specific probe for imaging application in living systems and quantitative analysis in environmental/food samples

Mercury ions are highly toxic and can accumulate along food chains in water,soil,crops and animals.Effective detection of mercury ions in various media is of great significance for maintaining the ecological environment and protecting people's health.In this work,a mercury ions specific fluorescent probe was developed by a simple one-step reaction of commercial substrates of 4-chloro-7-nitro-2,1,3-benzoxadiazole and 1-(2-aminoethyl)-4-methylpiperazine.Investigation on sensing behavior showed that this probe had high sensitivity and selectivity towards mercury ions.Furthermore,this probe could be used as a tool to track the level of mercury ions in living system.In living cells,the probe with green emission emitted a bright red fluorescence when it was bound to mercury ions.In Arabidopsis thaliana,similar red emission could be detected from the root tip and stalk when A.thaliana was grown in culture medium containing mercury ions.The imaging in zebrafish showed that mercury ions were mainly concentrated in the stomach and head of zebrafish.Especially,this probe could be applied in quantitative analysis of mercury ions in tap water,green tea,sea shrimp and soil.This work provided a practical tool for the detection of mercury ions in living systems and quantitative analysis in real samples.