Objective: To observe the effect of different combination antihypertensive therapies on plasma endothelin-1(ET-1) concentration and left ventricular diastolic function of essential hypertension patients with left vent...Objective: To observe the effect of different combination antihypertensive therapies on plasma endothelin-1(ET-1) concentration and left ventricular diastolic function of essential hypertension patients with left ventricular hypertrophy(LVH). Methods: A total of 111 patients with essential hypertension were selected, including 60 cases(34 males, 26 females) aged(60.7±5.6) years with simple hypertension, and 51 cases(28 males, 23 females) aged(61.8±7.0) years with essential hypertension complicated with LVH. Essential hypertension patients with LVH were randomly divided into the group of amlodipine(2.5 mg/d) combined with telmisartan(40 mg/d, n=26) and the group of amlodipine(2.5 mg/d) combined with amiloride(half tablet/d, n=25),and the treatment lasted for 1 year. Echocardiography was performed before and after the treatment. Left ventricular mass index(LVMI), left ventricular isovolumic relaxation time(IVRT) and other indicators were detected, and plasma ET-1 concentrations were measured for comparative analysis. And 56 patients(31 males, 25 females) aged(59.3±6.7) years with normal blood pressure in the same period in our hospital were selected as the normal control group. Results: The general clinical characteristics were similar between hypertensive LVH group, simple hypertensive group and normal healthy control group. Plasma ET-1 concentrations, LVMI and IVRT of hypertensive LVH group were significantly higher than those of normal control group and simple hypertension group, and the difference was statistically significant. The patients' baseline blood pressure, ET-1, LVMI, interventricular septal thickness(IVST), left ventricular posterior wall thickness(LVPWT), left ventricular end-diastolic diameter(LVEDd) and other clinical parameters showed no significant difference(P>0.05) between the group of amlodipine combined with telmisartan and the group of amlodipine combined with amiloride. It was found that compared with that before treatment, blood pressure could be effectively controlled(P<0.05), and LVMI, IVST, LVPWT and IVRT(P<0.05) were all lowered, and ET-1(P<0.01) was significantly reduced after 1 year of antihypertensive therapy in both the group of amlodipine combined with telmisartan and the group of amlodipine combined with amiloride. The group of amlodipine combined with telmisartan was better in lowering blood pressure and reducing LVMI, IVST, LVPWT, IVRT and ET-1 than the group of amlodipine combined with amiloride(P<0.05). Conclusion: Amlodipine-based combination antihypertensive therapy could reverse LVH and improve left ventricular diastolic function partly by lowering blood pressure and ET-1, and the effect of amlodipine combined with telmisartan was superior to that of amlodipine combined with amiloride.展开更多
Objective:To observe the expressions and clinical significance of plasma ET-1 and IL-8 in patients with AECOPD merged with CHD.Methods: A total of 120 patients with AECOPD who were admitted in our hospital from Februa...Objective:To observe the expressions and clinical significance of plasma ET-1 and IL-8 in patients with AECOPD merged with CHD.Methods: A total of 120 patients with AECOPD who were admitted in our hospital from February, 2015 to December, 2015 were included in the study and divided into observation 1 group (AECOPD merged with CHD) and observation 2 group (pure AECOPD) with 60 cases in each group. Moreover, 30 healthy individuals who came for physical examinations in the same period were served as the control group. ELISA was used to detect the plasma ET-1 and IL-8 levels, and their correlation was analyzed. The arterial blood gas and coagulation fibrinolysis indicators among the three groups were compared.Results: The plasma ET-1 and IL-8 levels in observation 1 and 2 groups were significantly higher than those in the control group. The plasma ET-1 and IL-8 levels in observation 1 group were significantly higher than those in observation 2 group. PaO2 in observation 1 and 2 groups was significantly lower than that in the control group, while PaCO2 was significantly higher than that in the control group. The comparison of PaO2 and PaCO2 between the two groups was not statistically significant. PT and APTT in observation 1 and 2 groups were significantly shortened when compared with the control group, while TT was significantly delayed. PT in observation 1 group was significantly shortened when compared with observation 2 group. Fbg and t-PA contents in observation 1 and 2 groups were significantly higher than those in the control group. Fbg, t-PA, and D-D contents in observation 1 group were significantly higher than those in observation 2 group.Conclusions:The hypercoagulation state is presenting in patients with AECOPD merged with CHD, with increased ET-1 and IL-8, both of which can play a synergistic effect, and are involved in the pathogenesis.展开更多
目的:探讨针灸在颈性眩晕治疗中的临床效果及对中医症状积分、C反应蛋白(C-reactive protein,CRP)、内皮素-1(Endothelin-1,ET-1)、降钙素基因相关肽(Calcitonin gene related peptide,CGRP)水平及生活质量的影响。方法:选取我院2020年...目的:探讨针灸在颈性眩晕治疗中的临床效果及对中医症状积分、C反应蛋白(C-reactive protein,CRP)、内皮素-1(Endothelin-1,ET-1)、降钙素基因相关肽(Calcitonin gene related peptide,CGRP)水平及生活质量的影响。方法:选取我院2020年3月至2021年11月期间收治的80例颈性眩晕患者作为研究对象。用随机数字法将其分对照组和观察组,每组各40例。对照组接受整脊法治疗,观察组在对照组基础上再接受针灸治疗。分析比较两组的疗效、中医症状积分、CRP、ET-1、CGRP水平以及生活质量。结果:观察组疗效显著高于对照组(P<0.05)。治疗前,两组的中医症状积分比较无显著差异(P>0.05)。治疗后,观察组视物模糊、头痛积分均显著低于对照组(P<0.05)。治疗前,两组的CRP、ET-1、CGRP指标水平比较无显著差异(P>0.05)。治疗后,观察组的CRP、ET-1均显著高于对照组,CGRP水平显著低于对照组(P<0.05)。治疗前,两组的SF-36评分比较无显著差异(P>0.05)。治疗后,观察组的SF-35评分显著高于对照组(P<0.05)。结论:颈性眩晕患者在接受整脊法治疗基础上,再给予针灸治疗,能提高临床疗效,改善CRP、ET-1、CGRP指标水平和临床症状,提高患者的生活质量。展开更多
AIM: To evaluate the effect of glycine on plasma and liver tissue changes of tumor necrosis factor-α (TNF-α),endothelin-1 (ET-1) and nitric oxide (NO) contents in rats with obstructive jaundice.METHODS: Ninety healt...AIM: To evaluate the effect of glycine on plasma and liver tissue changes of tumor necrosis factor-α (TNF-α),endothelin-1 (ET-1) and nitric oxide (NO) contents in rats with obstructive jaundice.METHODS: Ninety healthy Wistar rats of both sexes weighing 275±25 g were randomly divided into shamoperated, bile duct-ligated, and bile duct-ligated plus glycine-treated groups, the latter was performed with 5 %glycine solution substituting for tap water drunk ad libium for 5 days before and 6 days after operation. Blood and liver tissue were sampled at the time of sacrifice on the 8th day post operation. Plasma total bilirubin, endotoxin, levels,as well as TNF-α, ET-1 and NO contents in liver tissue were determined.RESULTS: Plasma endotoxin and total bilirubin levels were significantly higher in both bile duct-ligated and bile ductligated plus glycine-treated rats than in sham-operated animals (P=0.000613, 0.00921 and 0.00737, 0.00841respectively), whereas they did not display any statistically significant difference between the former groups (P=0.417and 0.374 respectively). Likewise, TNF-α, ET-1 and NO contents in both plasma and liver tissue were significantly increased in both bile duct-ligated and bile duct-ligated plus glycine-treated rats compared with sham-operated animals (P=-0.00813, 0.00793, 0.00671, 0.00804, 0.00872, and 0.00947 in plasma and 0.00531, 0.00785, 0.00912, 0.00981and 0.00635 in liver tissue respectively). However, these inflammatory mediators in both plasma and liver tissue were significantly reduced in bile duct-ligated rats fed on 5 %glycine solution compared with that without (P=0.00953,0.00891, 0.0795, 0.00867, 0.0697 and 0.00907 in plasma and liver tissue respectively).CONCLUSION: Reduction of TNF-α, ET-1 and NO contents in plasma and liver tissue of rats fed on glycine may be helpful to alleviate pathological lesions in obstructive jaundice.展开更多
BACKGROUND Previous studies have shown that the Shi-pi-xiao-ji(SPXJ)herbal decoction formula is effective in suppressing hepatocellular carcinoma(HCC),but the underlying mechanisms are not known.Therefore,this study i...BACKGROUND Previous studies have shown that the Shi-pi-xiao-ji(SPXJ)herbal decoction formula is effective in suppressing hepatocellular carcinoma(HCC),but the underlying mechanisms are not known.Therefore,this study investigated whether the antitumor effects of the SPXJ formula in treating HCC were mediated by acetyl-coA acetyltransferase 1(ACAT1)-regulated cellular stiffness.Through a series of experiments,we concluded that SPXJ inhibits the progression of HCC by upregulating the expression level of ACAT1,lowering the level of cholesterol in the cell membrane,and altering the cellular stiffness,which provides a new idea for the research of traditional Chinese medicine against HCC.AIM To investigate the anti-tumor effects of the SPXJ formula on the malignant progression of HCC.METHODS HCC cells were cultured in vitro with SPXJ-containing serum prepared by injecting SPXJ formula into wild-type mice.The apoptotic rate and proliferative,invasive,and migratory abilities of control and SPXJ-treated HCC cells were compared.Atomic force microscopy was used to determine the cell surface morphology and the Young’s modulus values of the control and SPXJ-treated HCC cells.Plasma membrane cholesterol levels in HCC cells were detected using the Amplex Red cholesterol detection kit.ACAT1 protein levels were estimated using western blotting.RESULTS Compared with the vehicle group,SPXJ serum considerably reduced proliferation of HCC cells,increased stiffness and apoptosis of HCC cells,inhibited migration and invasion of HCC cells,decreased plasma membrane cholesterol levels,and upregulated ACAT1 protein levels.However,treatment of HCC cells with the water-soluble cholesterol promoted proliferation,migration,and invasion of HCC cells as well as decreased cell stiffness and plasma membrane cholesterol levels,but did not alter the apoptotic rate and ACAT1 protein expression levels compared with the vehicle control.CONCLUSION SPXJ formula inhibited proliferation,invasion,and migration of HCC cells by decreasing plasma membrane cholesterol levels and altering cellular stiffness through upregulation of ACAT1 protein expression.展开更多
基金Supported by the National Natural and Science Foundation of China(30871042)
文摘Objective: To observe the effect of different combination antihypertensive therapies on plasma endothelin-1(ET-1) concentration and left ventricular diastolic function of essential hypertension patients with left ventricular hypertrophy(LVH). Methods: A total of 111 patients with essential hypertension were selected, including 60 cases(34 males, 26 females) aged(60.7±5.6) years with simple hypertension, and 51 cases(28 males, 23 females) aged(61.8±7.0) years with essential hypertension complicated with LVH. Essential hypertension patients with LVH were randomly divided into the group of amlodipine(2.5 mg/d) combined with telmisartan(40 mg/d, n=26) and the group of amlodipine(2.5 mg/d) combined with amiloride(half tablet/d, n=25),and the treatment lasted for 1 year. Echocardiography was performed before and after the treatment. Left ventricular mass index(LVMI), left ventricular isovolumic relaxation time(IVRT) and other indicators were detected, and plasma ET-1 concentrations were measured for comparative analysis. And 56 patients(31 males, 25 females) aged(59.3±6.7) years with normal blood pressure in the same period in our hospital were selected as the normal control group. Results: The general clinical characteristics were similar between hypertensive LVH group, simple hypertensive group and normal healthy control group. Plasma ET-1 concentrations, LVMI and IVRT of hypertensive LVH group were significantly higher than those of normal control group and simple hypertension group, and the difference was statistically significant. The patients' baseline blood pressure, ET-1, LVMI, interventricular septal thickness(IVST), left ventricular posterior wall thickness(LVPWT), left ventricular end-diastolic diameter(LVEDd) and other clinical parameters showed no significant difference(P>0.05) between the group of amlodipine combined with telmisartan and the group of amlodipine combined with amiloride. It was found that compared with that before treatment, blood pressure could be effectively controlled(P<0.05), and LVMI, IVST, LVPWT and IVRT(P<0.05) were all lowered, and ET-1(P<0.01) was significantly reduced after 1 year of antihypertensive therapy in both the group of amlodipine combined with telmisartan and the group of amlodipine combined with amiloride. The group of amlodipine combined with telmisartan was better in lowering blood pressure and reducing LVMI, IVST, LVPWT, IVRT and ET-1 than the group of amlodipine combined with amiloride(P<0.05). Conclusion: Amlodipine-based combination antihypertensive therapy could reverse LVH and improve left ventricular diastolic function partly by lowering blood pressure and ET-1, and the effect of amlodipine combined with telmisartan was superior to that of amlodipine combined with amiloride.
文摘Objective:To observe the expressions and clinical significance of plasma ET-1 and IL-8 in patients with AECOPD merged with CHD.Methods: A total of 120 patients with AECOPD who were admitted in our hospital from February, 2015 to December, 2015 were included in the study and divided into observation 1 group (AECOPD merged with CHD) and observation 2 group (pure AECOPD) with 60 cases in each group. Moreover, 30 healthy individuals who came for physical examinations in the same period were served as the control group. ELISA was used to detect the plasma ET-1 and IL-8 levels, and their correlation was analyzed. The arterial blood gas and coagulation fibrinolysis indicators among the three groups were compared.Results: The plasma ET-1 and IL-8 levels in observation 1 and 2 groups were significantly higher than those in the control group. The plasma ET-1 and IL-8 levels in observation 1 group were significantly higher than those in observation 2 group. PaO2 in observation 1 and 2 groups was significantly lower than that in the control group, while PaCO2 was significantly higher than that in the control group. The comparison of PaO2 and PaCO2 between the two groups was not statistically significant. PT and APTT in observation 1 and 2 groups were significantly shortened when compared with the control group, while TT was significantly delayed. PT in observation 1 group was significantly shortened when compared with observation 2 group. Fbg and t-PA contents in observation 1 and 2 groups were significantly higher than those in the control group. Fbg, t-PA, and D-D contents in observation 1 group were significantly higher than those in observation 2 group.Conclusions:The hypercoagulation state is presenting in patients with AECOPD merged with CHD, with increased ET-1 and IL-8, both of which can play a synergistic effect, and are involved in the pathogenesis.
文摘目的:探讨针灸在颈性眩晕治疗中的临床效果及对中医症状积分、C反应蛋白(C-reactive protein,CRP)、内皮素-1(Endothelin-1,ET-1)、降钙素基因相关肽(Calcitonin gene related peptide,CGRP)水平及生活质量的影响。方法:选取我院2020年3月至2021年11月期间收治的80例颈性眩晕患者作为研究对象。用随机数字法将其分对照组和观察组,每组各40例。对照组接受整脊法治疗,观察组在对照组基础上再接受针灸治疗。分析比较两组的疗效、中医症状积分、CRP、ET-1、CGRP水平以及生活质量。结果:观察组疗效显著高于对照组(P<0.05)。治疗前,两组的中医症状积分比较无显著差异(P>0.05)。治疗后,观察组视物模糊、头痛积分均显著低于对照组(P<0.05)。治疗前,两组的CRP、ET-1、CGRP指标水平比较无显著差异(P>0.05)。治疗后,观察组的CRP、ET-1均显著高于对照组,CGRP水平显著低于对照组(P<0.05)。治疗前,两组的SF-36评分比较无显著差异(P>0.05)。治疗后,观察组的SF-35评分显著高于对照组(P<0.05)。结论:颈性眩晕患者在接受整脊法治疗基础上,再给予针灸治疗,能提高临床疗效,改善CRP、ET-1、CGRP指标水平和临床症状,提高患者的生活质量。
文摘AIM: To evaluate the effect of glycine on plasma and liver tissue changes of tumor necrosis factor-α (TNF-α),endothelin-1 (ET-1) and nitric oxide (NO) contents in rats with obstructive jaundice.METHODS: Ninety healthy Wistar rats of both sexes weighing 275±25 g were randomly divided into shamoperated, bile duct-ligated, and bile duct-ligated plus glycine-treated groups, the latter was performed with 5 %glycine solution substituting for tap water drunk ad libium for 5 days before and 6 days after operation. Blood and liver tissue were sampled at the time of sacrifice on the 8th day post operation. Plasma total bilirubin, endotoxin, levels,as well as TNF-α, ET-1 and NO contents in liver tissue were determined.RESULTS: Plasma endotoxin and total bilirubin levels were significantly higher in both bile duct-ligated and bile ductligated plus glycine-treated rats than in sham-operated animals (P=0.000613, 0.00921 and 0.00737, 0.00841respectively), whereas they did not display any statistically significant difference between the former groups (P=0.417and 0.374 respectively). Likewise, TNF-α, ET-1 and NO contents in both plasma and liver tissue were significantly increased in both bile duct-ligated and bile duct-ligated plus glycine-treated rats compared with sham-operated animals (P=-0.00813, 0.00793, 0.00671, 0.00804, 0.00872, and 0.00947 in plasma and 0.00531, 0.00785, 0.00912, 0.00981and 0.00635 in liver tissue respectively). However, these inflammatory mediators in both plasma and liver tissue were significantly reduced in bile duct-ligated rats fed on 5 %glycine solution compared with that without (P=0.00953,0.00891, 0.0795, 0.00867, 0.0697 and 0.00907 in plasma and liver tissue respectively).CONCLUSION: Reduction of TNF-α, ET-1 and NO contents in plasma and liver tissue of rats fed on glycine may be helpful to alleviate pathological lesions in obstructive jaundice.
基金Supported by the National Natural Science Foundation of China,No.82074425Hunan Science and Technology Planning Project,No.2016SK2051 and No.2023SK2057the Hunan Provincial Administration of Traditional Chinese Medicine Research Project,No.B2023089.
文摘BACKGROUND Previous studies have shown that the Shi-pi-xiao-ji(SPXJ)herbal decoction formula is effective in suppressing hepatocellular carcinoma(HCC),but the underlying mechanisms are not known.Therefore,this study investigated whether the antitumor effects of the SPXJ formula in treating HCC were mediated by acetyl-coA acetyltransferase 1(ACAT1)-regulated cellular stiffness.Through a series of experiments,we concluded that SPXJ inhibits the progression of HCC by upregulating the expression level of ACAT1,lowering the level of cholesterol in the cell membrane,and altering the cellular stiffness,which provides a new idea for the research of traditional Chinese medicine against HCC.AIM To investigate the anti-tumor effects of the SPXJ formula on the malignant progression of HCC.METHODS HCC cells were cultured in vitro with SPXJ-containing serum prepared by injecting SPXJ formula into wild-type mice.The apoptotic rate and proliferative,invasive,and migratory abilities of control and SPXJ-treated HCC cells were compared.Atomic force microscopy was used to determine the cell surface morphology and the Young’s modulus values of the control and SPXJ-treated HCC cells.Plasma membrane cholesterol levels in HCC cells were detected using the Amplex Red cholesterol detection kit.ACAT1 protein levels were estimated using western blotting.RESULTS Compared with the vehicle group,SPXJ serum considerably reduced proliferation of HCC cells,increased stiffness and apoptosis of HCC cells,inhibited migration and invasion of HCC cells,decreased plasma membrane cholesterol levels,and upregulated ACAT1 protein levels.However,treatment of HCC cells with the water-soluble cholesterol promoted proliferation,migration,and invasion of HCC cells as well as decreased cell stiffness and plasma membrane cholesterol levels,but did not alter the apoptotic rate and ACAT1 protein expression levels compared with the vehicle control.CONCLUSION SPXJ formula inhibited proliferation,invasion,and migration of HCC cells by decreasing plasma membrane cholesterol levels and altering cellular stiffness through upregulation of ACAT1 protein expression.
