The finite-difference-time-domain （FDTD） method is applied to simulate the twodimensional propagation of electromagnetic TM （S-polarization） mode in atmospheric plasma and in metal layer for strong electron-neutra...The finite-difference-time-domain （FDTD） method is applied to simulate the twodimensional propagation of electromagnetic TM （S-polarization） mode in atmospheric plasma and in metal layer for strong electron-neutral collisions. Dependence of the wave attenuation on both plasma parameters and incident wave angle are obtained. It is indicated that for a given electron density profile the attenuation depends strongly on the incident angle, the wave frequency, the width of plasma layer, and the collision frequency between electrons and neutrals.展开更多
TM-2 known as a potential antitumor drug is a novel semi-synthetic taxane derivative. As drug-protein interactions contribute to insights into pharmacokinetic and pharmacodynamic properties, we eluci- dated the bindin...TM-2 known as a potential antitumor drug is a novel semi-synthetic taxane derivative. As drug-protein interactions contribute to insights into pharmacokinetic and pharmacodynamic properties, we eluci- dated the binding of TM-2 to plasma protein. In this study, a simple, rapid and reliable method was developed and validated employing equilibrium dialysis for the separation of bound and unbound drugs and ultra-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） for the quantitation. Protein binding reached equilibrium within 24 h of incubation at 37 ℃. After liquid-liquid extraction with methyl tert-butyl ether, the samples were separated on Thermo Syncronis UPLC C18 （2.1 mm× 50 mm, 1.7 μm）, and acquisition of mass spectrometric data was performed in multiple re- action monitoring （MRM） mode via positive electrospray ionization. The assay was linear over the concentration rang of 5-2000 nglmL The intra- and inter-day precisions were 0.1%-14.8%, and the accuracy was from -6.4% to Z0%. This assay has been successfully applied to a protein binding study of TM-2 in rat, human and beagle dog plasma. TM-2 showed high protein binding of 81.4% ± 6.5% （rat）, 87.9% ± 3.6% （human） and 79.4% ± 4.0% （beagle dog）. The results revealed that there was an insignificant difference among the three species.展开更多
目的探讨妊娠11~14周孕妇血清中PAPP-A水平值在胎盘早剥中的预测价值,以及联合检测孕妇血清中CA125、TM水平对早期诊断胎盘早剥的临床价值。方法前瞻性选取2014年1月到2015年8月,孕11~14周时行Ⅰ期唐氏筛查的孕妇,孕周大于20周后有阴道...目的探讨妊娠11~14周孕妇血清中PAPP-A水平值在胎盘早剥中的预测价值,以及联合检测孕妇血清中CA125、TM水平对早期诊断胎盘早剥的临床价值。方法前瞻性选取2014年1月到2015年8月,孕11~14周时行Ⅰ期唐氏筛查的孕妇,孕周大于20周后有阴道出血、子宫体压痛、腰背痛、不明原因胎儿窘迫及宫缩过频、子宫张力过高等怀疑胎盘早剥的孕妇100例作为研究对象,产后检查是否有胎盘早剥再分为早剥组66例、无早剥组34例;同期选择在该院产检并正常分娩的100例孕妇为对照组,分别登记其PAPP-A值,并行CA125、TM检测,B超检查和产后胎盘检查,比较三组检测结果的差异。应用ROC曲线和约登指数评判各血清学指标在胎盘早剥诊断中的价值,并确定PAPP-A和TM相应的诊断界值。结果妊娠11~14周血清PAPP-A值,胎盘早剥组明显低于无胎盘早剥组和正常对照组（P〈0.05）,胎盘早剥组CA-125、TM水平明显高于无胎盘早剥组和正常对照组,均有统计学意义（P〈0.05）。将PAPP-A、TM作为检验变量得出诊断胎盘早剥的界值,PAPP-A〈2 028 m U/L、TM〉55μg/L时预测价值最高。结论妊娠11~14周低水平的血清PAPP-A值对胎盘早剥有预测作用;高水平的血清CA-125和高TM值对胎盘早剥的早期诊断具有较高的临床应用价值。展开更多
基金supported by National Natural Science Foundation of China(No. 90405004)
文摘The finite-difference-time-domain （FDTD） method is applied to simulate the twodimensional propagation of electromagnetic TM （S-polarization） mode in atmospheric plasma and in metal layer for strong electron-neutral collisions. Dependence of the wave attenuation on both plasma parameters and incident wave angle are obtained. It is indicated that for a given electron density profile the attenuation depends strongly on the incident angle, the wave frequency, the width of plasma layer, and the collision frequency between electrons and neutrals.
基金partly supported by the National High Technology Research and Development Program of China(No.2012AA020305)
文摘TM-2 known as a potential antitumor drug is a novel semi-synthetic taxane derivative. As drug-protein interactions contribute to insights into pharmacokinetic and pharmacodynamic properties, we eluci- dated the binding of TM-2 to plasma protein. In this study, a simple, rapid and reliable method was developed and validated employing equilibrium dialysis for the separation of bound and unbound drugs and ultra-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） for the quantitation. Protein binding reached equilibrium within 24 h of incubation at 37 ℃. After liquid-liquid extraction with methyl tert-butyl ether, the samples were separated on Thermo Syncronis UPLC C18 （2.1 mm× 50 mm, 1.7 μm）, and acquisition of mass spectrometric data was performed in multiple re- action monitoring （MRM） mode via positive electrospray ionization. The assay was linear over the concentration rang of 5-2000 nglmL The intra- and inter-day precisions were 0.1%-14.8%, and the accuracy was from -6.4% to Z0%. This assay has been successfully applied to a protein binding study of TM-2 in rat, human and beagle dog plasma. TM-2 showed high protein binding of 81.4% ± 6.5% （rat）, 87.9% ± 3.6% （human） and 79.4% ± 4.0% （beagle dog）. The results revealed that there was an insignificant difference among the three species.
文摘目的探讨妊娠11~14周孕妇血清中PAPP-A水平值在胎盘早剥中的预测价值,以及联合检测孕妇血清中CA125、TM水平对早期诊断胎盘早剥的临床价值。方法前瞻性选取2014年1月到2015年8月,孕11~14周时行Ⅰ期唐氏筛查的孕妇,孕周大于20周后有阴道出血、子宫体压痛、腰背痛、不明原因胎儿窘迫及宫缩过频、子宫张力过高等怀疑胎盘早剥的孕妇100例作为研究对象,产后检查是否有胎盘早剥再分为早剥组66例、无早剥组34例;同期选择在该院产检并正常分娩的100例孕妇为对照组,分别登记其PAPP-A值,并行CA125、TM检测,B超检查和产后胎盘检查,比较三组检测结果的差异。应用ROC曲线和约登指数评判各血清学指标在胎盘早剥诊断中的价值,并确定PAPP-A和TM相应的诊断界值。结果妊娠11~14周血清PAPP-A值,胎盘早剥组明显低于无胎盘早剥组和正常对照组（P〈0.05）,胎盘早剥组CA-125、TM水平明显高于无胎盘早剥组和正常对照组,均有统计学意义（P〈0.05）。将PAPP-A、TM作为检验变量得出诊断胎盘早剥的界值,PAPP-A〈2 028 m U/L、TM〉55μg/L时预测价值最高。结论妊娠11~14周低水平的血清PAPP-A值对胎盘早剥有预测作用;高水平的血清CA-125和高TM值对胎盘早剥的早期诊断具有较高的临床应用价值。
