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miR-374b、miR-765、miR-126在2型糖尿病变化中的相关性分析
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作者 李志健 曹长峰 王佩佩 《齐齐哈尔医学院学报》 2023年第16期1525-1528,共4页
目的 研究miR-374b、miR-765、miR-126在2型糖尿病变化中的相关性分析。方法 选择2020年3月—2022年3月于本院行健康体检的健康志愿者50名作为健康组,并将同期收治的102例2型糖尿病患者作为疾病组,采用荧光定量PCR法检测所有研究对象miR... 目的 研究miR-374b、miR-765、miR-126在2型糖尿病变化中的相关性分析。方法 选择2020年3月—2022年3月于本院行健康体检的健康志愿者50名作为健康组,并将同期收治的102例2型糖尿病患者作为疾病组,采用荧光定量PCR法检测所有研究对象miR-374b、miR-765、miR-126表达量。对比疾病组、健康组各指标表达水平、各指标在疾病组疾病不同时期、不同严重程度中的表达,并分析miR-374b、miR-765、miR-126在2型糖尿病变化中的相关性。结果 在miR-374b、miR-765水平上,疾病组高于健康组,在miR-126水平上,疾病组低于健康组(P<0.05);随着疾病的加重,患者miR-374b、miR-765水平逐渐升高,重度>中度>轻度,miR-126水平逐渐下降,重度<中度<轻度(P<0.05);相关性分析发现miR-374b与miR-765均为正相关;miR-765与miR-126为负相关,miR-374b与miR-126均负相关,(均P<0.05)。结论 miR-126在疾病中均为低表达,miR-374b、miR-765在疾病中均为高表达,检测miR-374b、miR-765、miR-126水平有助于评价2型糖尿病患者疾病严重程度。 展开更多
关键词 mir-374b mir-765 mir-126 2型糖尿病 疾病严重程度
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Plasma miR-216a as a potential marker of pancreatic injury in a rat model of acute pancreatitis 被引量:19
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作者 Xiang-Yu Kong,Yi-Qi Du,Lei Li,Xiao-Hua Man,Yan-Fang Gong,Li-Ning Xiao,Yong-Zhi Zheng,Shang-Xin Deng,Jun-Jun Gu,Zhao-Shen Li,Department of Gastroenterology,Changhai Hospital,Second Military Medical University,Shang-hai 200433,China Jian-Qiang Liu,Department of Gastroenterology,Fuzhou General Hospital of Nanjing Military Command,Fuzhou 350025,Fujian Province,China Guo-Kun Wang,Jia-Qi Zhu,Department of Cardiology,Changhai Hospital,Second Military Medical University,Shang-hai 200433,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2010年第36期4599-4604,共6页
AIM:To study the potential value and specificity of plasma miR-216a as a marker for pancreatic injury.METHODS:Two rat models were applied in this article:L-arginine-induced acute pancreatitis was used as one model to ... AIM:To study the potential value and specificity of plasma miR-216a as a marker for pancreatic injury.METHODS:Two rat models were applied in this article:L-arginine-induced acute pancreatitis was used as one model to explore the potential value of plasma miR-216a for detection of pancreatic injury;nonlethal sepsis induced in rats by single puncture cecal ligation and puncture(CLP)was used as the other model to evaluate the specificity of plasma miR-216a compared with twocommonly used markers(amylase and lipase)for acute pancreatitis.Plasmas were sampled from rats at indicated time points and total RNA was isolated.Real-Time Quantitative reverse transcriptase-polymerase chain reaction was used to quantify miR-216a in plasmas.RESULTS:In the acute pancreatitis model,among five time points at which plasmas were sampled,miR-216a concentrations were significantly elevated 24 h after arginine administration and remained significantly increased until 48 h after operation(compared with 0 h time point,P<0.01,Kruskal-Wallis Test).In the CLP model,plasma amylase and lipase,two commonly used biomarkers for acute pancreatitis,were significantly elevated 24 h after operation(compared with 0 h time point,P<0.01 and 0.05 respectively,Pairwise Bonferroni corrected ttests),while miR-216a remained undetectable among four tested time points.CONCLUSION:Our article showed for the first time that plasma miR-216a might serve as a candidate marker of pancreatic injury with novel specificity. 展开更多
关键词 mir-216a plasma miRNA PANCREATIC INJURY Acute PANCREATITIS BIOMARKER
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冠心病合并2型糖尿病患者血浆miR-765水平、临床意义及靶基因功能研究 被引量:4
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作者 付霞 罗茂 《中国动脉硬化杂志》 CAS 2021年第5期405-411,共7页
目的调查冠心病(CHD)合并2型糖尿病(T2DM)患者血浆miR-765水平变化及其靶基因功能,探讨血浆miR-765作为CHD合并T2DM潜在生物标志物的临床意义。方法收集本院行冠状动脉造影术确诊为CHD的患者56例,根据是否合并T2DM分为两组:单纯CHD组(CH... 目的调查冠心病(CHD)合并2型糖尿病(T2DM)患者血浆miR-765水平变化及其靶基因功能,探讨血浆miR-765作为CHD合并T2DM潜在生物标志物的临床意义。方法收集本院行冠状动脉造影术确诊为CHD的患者56例,根据是否合并T2DM分为两组:单纯CHD组(CHD组,33例)、CHD合并T2DM组(CHD+T2DM组,23例),另设冠状动脉造影结果阴性者为对照组(30例)。采用Gensini评分系统评价冠状动脉狭窄程度。实时荧光定量PCR检测血浆miR-765水平。生物信息学分析方法预测miR-765靶基因,并且进行靶基因Gene Ontology(GO)功能注释及编码蛋白质间相互作用(PPI)网络构建。结果与CHD组比较,CHD+T2DM组Gensini评分显著增高。与对照组比较,CHD组血浆miR-765水平显著升高(P<0.05);与CHD组比较,CHD+T2DM组血浆miR-765水平显著升高(P<0.05)。靶基因生物信息学分析共获得30个潜在靶标基因,生物学功能、分子功能和细胞组成分类分析结果表明,miR-765参与调节细胞形态建成与趋化、炎症、免疫、稳态与转运和蛋白质氨基酸磷酸化等进程,提示miR-765可能调控趋化因子信号通路、钙信号途径和磷脂酰肌醇信号通路。进一步的PPI网络分析发现miR-765靶基因编码蛋白质间具有复杂的相互作用。结论CHD合并T2DM患者血浆miR-765水平显著升高,血浆miR-765是CHD合并T2DM患者潜在的临床诊断标志物,对CHD合并T2DM具有较高的诊断价值。 展开更多
关键词 冠心病合并2型糖尿病 血浆mir-765 临床意义 生物信息学分析
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血清miR-424及miR-765在多发性骨髓瘤中的表达及其临床意义 被引量:5
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作者 黄娟娟 卓芬 +1 位作者 蔡月红 肖华 《中国实验血液学杂志》 CAS CSCD 北大核心 2022年第2期461-465,共5页
目的:探讨多发性骨髓瘤(MM)患者血清miR-424及miR-765的表达及其临床意义。方法:选取2017年1月-2020年7月三亚中心医院收治的81例MM患者,依据国际分期系统分为Ⅰ期(n=16)、Ⅱ期(n=25)和Ⅲ期(n=40);根据免疫分型结果分为IgG型(n=46)、IgA... 目的:探讨多发性骨髓瘤(MM)患者血清miR-424及miR-765的表达及其临床意义。方法:选取2017年1月-2020年7月三亚中心医院收治的81例MM患者,依据国际分期系统分为Ⅰ期(n=16)、Ⅱ期(n=25)和Ⅲ期(n=40);根据免疫分型结果分为IgG型(n=46)、IgA型(n=19)、轻链型(n=10)和非分泌型(n=6)。另选取同期50例健康体检正常者作为对照组。检测各组血清miR-424、miR-765及胱抑素C(Cys-C)水平。应用受试者工作特征(ROC)曲线分析血清miR-424、miR-765及Cys-C水平诊断MM的价值。采用Pearson相关分析MM患者血清miR-424、miR-765水平与Cys-C的相关性。结果:MM组血清miR-424(2.74±1.30 vs 0.85±0.26)、miR-765(2.05±0.82 vs 0.63±0.17)及Cys-C[(2.18±0.86 vs 0.72±0.15)mg/L]水平均明显高于对照组(P<0.001)。MMⅢ期患者血清miR-424(5.08±2.36 vs 1.12±0.34,2.24±0.93)、miR-765(3.50±1.52 vs 0.74±0.20,1.78±0.65)及Cys-C[(3.81±1.30 vs 0.92±0.24,1.68±0.55) mg/L]水平均明显高于Ⅰ期和Ⅱ期(P<0.001),且Ⅱ期患者血清miR-424、miR-765及Cys-C水平均明显高于Ⅰ期(P<0.001)。MM IgG型患者血清miR-424及miR-765水平均明显高于IgA、轻链和非分泌型(P<0.001)。ROC曲线分析结果显示,miR-424、miR-765、Cys-C及3项联合诊断MM的曲线下面积以后者为最大(0.952,95%CI:0.890-0.993),其敏感度和特异度为95.0%和87.2%。相关分析结果显示,MM患者血清miR-424及miR-765水平与Cys-C均呈正相关(r=0.795,r=0.760)。结论:MM患者血清miR-424及miR-765水平明显升高,且随着MM病情分期呈增高趋势,联合Cys-C检测对MM诊断具有较高的价值。 展开更多
关键词 多发性骨髓瘤 mir-424 mir-765 临床意义
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LncRNA BDNF-AS靶向miR-765影响缺氧缺糖诱导的PC12细胞神经损伤的实验研究
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作者 蒋邦治 唐永刚 +2 位作者 韩志安 陈林坤 韦家俊 《河北医药》 CAS 2022年第20期3075-3078,3083,共5页
目的探讨LncRNA BDNF-AS对缺氧缺糖诱导的PC12细胞神经损伤的影响及分子机制。方法PC12细胞分为对照组、模型组、si-LncRNA BDNF-AS+模型组、si-NC+模型组、miR-765+模型组、miR-NC+模型组、anti-miR-NC+si-LncRNA BDNF-AS+模型组、anti... 目的探讨LncRNA BDNF-AS对缺氧缺糖诱导的PC12细胞神经损伤的影响及分子机制。方法PC12细胞分为对照组、模型组、si-LncRNA BDNF-AS+模型组、si-NC+模型组、miR-765+模型组、miR-NC+模型组、anti-miR-NC+si-LncRNA BDNF-AS+模型组、anti-miR-765+si-LncRNA BDNF-AS+模型组;实时荧光定量PCR(RT-qPCR)检测LncRNA BDNF-AS和miR-765的表达水平;流式细胞术检测细胞凋亡;蛋白质印迹法检测蛋白表达;酶联免疫吸附法(ELISA)检测丙二醛(MDA)、超氧化歧化酶(SOD)、过氧化氢酶(CAT)水平;双荧光素酶报告实验检测LncRNA BDNF-AS和miR-765的靶向关系。结果与对照组比较,模型组LncRNA BDNF-AS表达水平升高,miR-765表达水平降低,PC12细胞凋亡率以及Cleaved-caspase3、Bax表达水平升高,MDA含量升高,SOD和CAT活性降低(P<0.05)。抑制LncRNA BDNF-AS表达或过表达miR-765后,PC12细胞凋亡率、Cleaved-caspase3和Bax表达水平降低,MDA含量降低,SOD和CAT活性升高(P<0.05)。LncRNA BDNF-AS靶向调控miR-765;抑制miR-765可以逆转低表达LncRNA BDNF-AS对缺氧缺糖诱导的PC12细胞神经损伤的影响。结论抑制LncRNA BDNF-AS表达通过靶向调控miR-765抑制缺氧缺糖诱导的PC12细胞神经损伤。 展开更多
关键词 LncRNA BDNF-AS mir-765 缺氧缺糖 PC12细胞 神经损伤
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lncRNA NCRNA00173靶向miR-765对骨肉瘤细胞增殖、迁移和侵袭的影响的实验研究 被引量:1
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作者 吴昌强 韦敏 +1 位作者 郑一鸣 林正坚 《广西医科大学学报》 CAS 2021年第9期1748-1754,共7页
目的:探讨长链非编码RNA(lncRNA)NCRNA00173是否通过靶向调控miR-765影响骨肉瘤细胞增殖、迁移和侵袭。方法:采用实时荧光定量PCR(q PCR)检测骨肉瘤细胞U2-OS、Saos-2、SJSA-1和SOSP-9607以及人成骨细胞hFOB1.19中NCRNA00173和miR-765... 目的:探讨长链非编码RNA(lncRNA)NCRNA00173是否通过靶向调控miR-765影响骨肉瘤细胞增殖、迁移和侵袭。方法:采用实时荧光定量PCR(q PCR)检测骨肉瘤细胞U2-OS、Saos-2、SJSA-1和SOSP-9607以及人成骨细胞hFOB1.19中NCRNA00173和miR-765的表达情况。选择骨肉瘤U2-OS细胞为研究对象,将细胞随机分为Con组(正常培养的U2-OS细胞)、pcDNA组(转染空载体质粒)和NCRNA00173组(转染pcDNA-NCRNA00173过表达载体质粒)。qPCR检测NCRNA00173和miR-765的表达变化;双荧光素酶报告基因实验检测NCRNA00173是否靶向miR-765;分别采用噻唑蓝(MTT)法、克隆形成实验、划痕实验和Transwell实验检测过表达NCRNA00173对U2-OS细胞增殖、克隆形成、迁移和侵袭能力的影响,蛋白质印迹(Western blotting)检测增殖细胞核抗原(PCNA)、基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶-9(MMP-9)的表达。结果:与人成骨细胞h FOB1.19相比,骨肉瘤细胞U2-OS、Saos-2、SJSA-1和SOSP-9607中NCRNA00173的表达明显下调(P<0.05),而miR-765的表达明显上调(P<0.05);与Con组和pcDNA组相比,NCRNA00173组细胞中NCRNA00173的表达显著升高(P<0.05),而miR-765、PCNA、MMP-2和MMP-9的表达显著降低(P<0.05),细胞增殖和克隆形成能力降低(P<0.05),划痕愈合率明显降低(P<0.05),迁移和侵袭细胞数明显减少(P<0.05);双荧光素酶报告基因实验证实NCRNA00173可靶向结合miR-765。结论:NCRNA00173可通过靶向miR-765影响骨肉瘤细胞增殖、迁移和侵袭。 展开更多
关键词 lncRNA NCRNA00173 mir-765 骨肉瘤细胞 增殖 迁移
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lncRNA RP11-351J23.1靶向miR-765对食管鳞癌细胞增殖、侵袭和迁移的影响及其机制研究
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作者 康书红 蔺红丽 赵霞 《实验与检验医学》 CAS 2021年第4期762-767,共6页
目的研究lncRNA RP11-351J23.1对食管鳞癌细胞增殖、侵袭和迁移的影响和潜在机制。方法以正常食管上皮细胞HEEC为对照,qRT-PCR检测食管鳞癌Eca109、KYSE150和EC9706细胞中lncRNA RP11-351J23.1和miR-765表达量,用分子生物学方法上调或下... 目的研究lncRNA RP11-351J23.1对食管鳞癌细胞增殖、侵袭和迁移的影响和潜在机制。方法以正常食管上皮细胞HEEC为对照,qRT-PCR检测食管鳞癌Eca109、KYSE150和EC9706细胞中lncRNA RP11-351J23.1和miR-765表达量,用分子生物学方法上调或下调Eca109细胞中RP11-351J23.1和miR-765表达量,CCK8法和Transwell实验分别检测Eca109细胞增殖、侵袭和迁移能力,双荧光素酶报告系统验证RP11-351J23.1和miR-765的调控关系。结果与对照相比,在食管癌Eca109、KYSE150和EC9706细胞中lncRNA RP11-351J23.1表达量均显著降低(P<0.05),miR-765表达量均显著升高(P<0.05);过表达lncRNA RP11-351J23.1可抑制Eca109细胞增殖、侵袭和迁移;lncRNA RP11-351J23.1靶向miR-765,抑制miR-765的表达;抑制miR-765表达可抑制Eca109细胞增殖、侵袭和迁移;过表达miR-765可逆转过表达lncRNA RP11-351J23.1对Eca109细胞增殖、侵袭和迁移的抑制作用。结论LncRNA RP11-351J23.1在食管鳞癌细胞中表达下调,过表达lncRNA RP11-351J23.1可靶向miR-765抑制食管鳞癌Eca109细胞增殖、侵袭和迁移。LncRNA RP11-351J23.1可能是食管鳞癌的潜在分子靶点。 展开更多
关键词 食管癌 RP11-351J23.1 mir-765 增殖 迁移 侵袭
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重型颅脑外伤患者血清MicroRNA765水平变化及意义 被引量:1
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作者 秦国强 黄卫民 +3 位作者 施波 彭才祖 王冠 严程芬 《山东医药》 CAS 2013年第36期44-46,共3页
目的观察重型颅脑外伤(sTBI)患者血清MicroRNA765(miR-765)水平变化,并探讨其意义。方法应用实时荧光定量PCR检测10例健康志愿者(HV组)及sTBI(STBI组)、轻型颅脑外伤(mTBI组)、四肢骨折(FRA组)各10例患者的血清miR-765。结果 sTBI组血清... 目的观察重型颅脑外伤(sTBI)患者血清MicroRNA765(miR-765)水平变化,并探讨其意义。方法应用实时荧光定量PCR检测10例健康志愿者(HV组)及sTBI(STBI组)、轻型颅脑外伤(mTBI组)、四肢骨折(FRA组)各10例患者的血清miR-765。结果 sTBI组血清miR-765水平较其他3组升高(P均<0.05),而mTBI组相对FRA及HV组无明显改变(P均>0.05)。sTBI组miR-765 ROC曲线下面积为0.88,诊断sTBI准确性为100%,诊断特异性为100%。结论 sTBI患者血清miR-765水平升高,其可能成为诊断sTBI的一种新型生化标志物。 展开更多
关键词 重型颅脑外伤 mir-765 实时定量PCR ROC曲线
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重型颅脑外伤患者血清MicroRNA765的表达与预后的研究
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作者 秦国强 黄卫民 +4 位作者 彭才祖 施波 王冠严 程芬 王淳良 《临床神经外科杂志》 CAS 2014年第5期367-370,共4页
目的检测血清MicroRNA765(miR-765)的表达与重型颅脑外伤(sTBI)患者预后的关系。方法应用实时定量PCR检测健康对照组(CTL)、sTBI组监测患者伤后第1、4、7天血清miR-765水平。结果sTBI组血清miR-765表达水平相对CTL组有显著改变(P<0.0... 目的检测血清MicroRNA765(miR-765)的表达与重型颅脑外伤(sTBI)患者预后的关系。方法应用实时定量PCR检测健康对照组(CTL)、sTBI组监测患者伤后第1、4、7天血清miR-765水平。结果sTBI组血清miR-765表达水平相对CTL组有显著改变(P<0.05),患者miR-765第一天开始上升,第4天达峰值水平,第7天明显下降,并与脑外伤后6个月GOS评分显著负相关,也与入院时GCS评分负相关。结论sTBI患者血清miR-765z早期变化可以作为评价患者预后的重要生化指标之一。 展开更多
关键词 重型颅脑外伤 mir-765 预后
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Reduction of Plasma MicroRNA-21 is Associated with Chemotherapeutic Response in Patients with Non-small Cell Lung Cancer 被引量:9
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作者 Juan Wei Lian-ke Liu Wen Gao Cheng-jun Zhu Yi-qian Liu Ting Cheng Yong-qian Shu 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2011年第2期123-128,共6页
Objective: To examine plasma microRNA-21 (miR-21) level in patients with non-small cell lung cancer (NSCLC) and its potential correlation with chemotherapeutic response. Methods: 77 NSCLC patients and 36 age and... Objective: To examine plasma microRNA-21 (miR-21) level in patients with non-small cell lung cancer (NSCLC) and its potential correlation with chemotherapeutic response. Methods: 77 NSCLC patients and 36 age and sex-matched healthy controls were included. Plasma miR-21 concentration was examined using a quantitative real-time reverse transcription polymerase chain reaction assay (qRT-PCR). Potential correlation between plasma mir-21 concentrations with chemotherapeutic responses was analyzed in 35 patients with advanced NSCLC (stages IIIB and IV). Results: Plasma miR-21 was significantly higher in NSCLC patients relative to the healthy controls (P0.0001). As a biomarker, plasma mir-21 had a receiver operating characteristic (ROC) curve area of 0.729 with 61.04% sensitivity and 83.33% specificity. Chemotherapeutic response in the 35 patients with advanced NSCLC (stages IIIB and IV) included partial response (PR) (n=11), stable disease and progression disease (SD+PD) (n=24). The overall response rate (CR+PR) was 31.4%. Plasma miR-21 in patients who achieved PR was significantly lower than those who did not respond (SD+PD) (P=0.0487), and comparable to that of the healthy controls (P=0.2744). Conclusion: Plasma miR-21 is a good biomarker for NSCLC, and could be used to predict responses to chemotherapy. 展开更多
关键词 NSCLC mir-21 BIOMARKER plasma Chemotherapeutic response
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miR-93-5p Transferred by Exosomes Promotes the Proliferation of Esophageal Cancer Cells via Intercellular Communication by Targeting PTEN 被引量:14
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作者 LIU Meng Xin LIAO Juan +7 位作者 XIE Ming GAO Zhi Kui WANG Xiang Hu ZHANG Ying SHANG Mu He YIN Li Hong PU Yue Pu LIU Ran 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2018年第3期171-185,共15页
Objective To investigate the relationship between plasma miR-93-5p and the risk of esophageal cancer, as well as the influence of miR-93-5p on the biological function of esophageal cancer cells,exerted through exosome... Objective To investigate the relationship between plasma miR-93-5p and the risk of esophageal cancer, as well as the influence of miR-93-5p on the biological function of esophageal cancer cells,exerted through exosomes.Methods The expression of plasma miR-93-5p in esophageal cancer patients and healthy controls was analysed by real-time quantitative PCR. The influence of miR-93-5p on the risk and prognosis of esophageal carcinoma was analyzed by conditional logistic regression and survival analysis. The effect of miR-93-5p on the biological function of recipient cells was investigated by establishing an in vitro donor cell co-culture model. The target gene of miR-93-5p was validated by luciferase reporter assay and Western Blotting.Results Upregulation of plasma miR-93-5p expression significantly increases the risk of esophageal cancer and is associated with poor prognosis. miR-93-5p transferred by exosomes promotes the proliferation of recipient esophageal cancer cells and affects the expression of PTEN and its downstream proteins p21 and cyclin D1.Conclusion Our study provides a reference for the identification of biomarkers for the diagnosis and prognosis of esophageal cancer. 展开更多
关键词 EXOSOMES mir-93-5p Esophageal cancer plasma biomarker
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miR-765通过Wnt/β-catenin信号通路调控甲状腺乳头状癌细胞生物学行为的研究 被引量:1
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作者 李锐 刘宏宇 +1 位作者 张洋 盖保东 《中华内分泌外科杂志》 CAS 2023年第4期430-434,共5页
目的探究miR-765在甲状腺乳头状癌(papillarythyroidcarcinoma,PTC)细胞中的作用及相关信号通路机制。方法利用qPCR检测miR-765在正常人甲状腺细胞系Nthy-ori3-1及人PTC细胞系B-CPAP和TPC-1中的表达。将PTC细胞分成空白对照组(blank con... 目的探究miR-765在甲状腺乳头状癌(papillarythyroidcarcinoma,PTC)细胞中的作用及相关信号通路机制。方法利用qPCR检测miR-765在正常人甲状腺细胞系Nthy-ori3-1及人PTC细胞系B-CPAP和TPC-1中的表达。将PTC细胞分成空白对照组(blank control,BC)、阴性对照组(negative control,NC)和miR-mimic组,BC组不做特殊处理,NC组转染阴性对照序列,miRNA模拟物(miR-mimic)组转染miR-765模拟物上调其表达。分别利用CCK-8实验、平板克隆形成实验、划痕实验和Transwell侵实验检测转染miR-mimic后PTC细胞的增殖、迁移及侵袭能力。利用Westernblot实验检测转染miR-mimic后PTC细胞中Wnt/β-catenin信号通路关键蛋白β-catenin的核内表达。结果与Nthy-ori 3-1细胞相比,PTC细胞中的miR-765表达显著下降(B-CPAP,P=0.0003;TPC-1,P=0.0003)。转染miR-mimic可显著上调PTC细胞中miR-765的表达(B-CPAP,P<0.0001;TPC-1,P<0.0001)。CCK-8实验(B-CPAP,P<0.05;TPC-1,P<0.05)、平板克隆形成实验(B-CPAP,P=0.0001;TPC-1,P<0.0001)、划痕实验(B-CPAP,P=0.0006;TPC-1,P<0.0001)及Tran-Swell侵袭实验(B-CPAP,P=0.001;TPC-1,P=0.0014)结果显示,上调miR-765的表达可显著抑制PTC细胞的增殖、迁移和侵袭能力。Western实验结果显示上调miR-765的表达可显著抑制PTC细胞中Wnt/β-catenin信号通路的水平(B-CPAP,P=0.0039;TPC-1,P=0.0004)。结论上调miR-765可抑制Wnt/β-catenin信号通路并抑制PTC细胞的增殖、迁移和侵袭能力,这不仅说明miR-765是PTC治疗的潜在新靶点,还进一步揭示了其发挥调控作用的机制。 展开更多
关键词 mir-765 Wnt/β-catenin信号通路 甲状腺乳头状癌 肿瘤细胞表型
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Seminal plasma miR-192a: a biomarker predicting successful resolution of nonobstructive azoospermia following varicocele repair 被引量:3
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作者 Er-Lei Zhi Guo-Qing Liang +4 位作者 Peng Li Hui-Xing Chen Ru-Hui Tian Peng Xu Zheng Li 《Asian Journal of Andrology》 SCIE CAS CSCD 2018年第4期396-399,共4页
This study was performed to investigate a potential marker for the presence of spermatozoa in the ejaculate following varicocelectomy in Chinese men with nonobstructive azoospermia and varicoceles. The micro-RNA (miR... This study was performed to investigate a potential marker for the presence of spermatozoa in the ejaculate following varicocelectomy in Chinese men with nonobstructive azoospermia and varicoceles. The micro-RNA (miR)-192a levels in seminal plasma and testicular tissue were evaluated by quantitative real-time polymerase chain reaction from 60 men with nonobstructive azoospermia and varicoceles (Group A: 27 men with spermatozoa found in the ejaculate after surgery; Group B: 33 men without spermatozoa found in the ejaculate after surgery) and 30 controls. The seminal plasma and testicular tissue miR-192a levels were higher in Group B than in Group A and the controls (P〈 0.001), and there was no significant difference between Group A and the controls (P〉 0.05). Apoptosis and proliferation assays with miR mimics and inhibitors showed that miR-192a induced GC-2 cell apoptosis through the activation of Caspase-3 protein. Thus, seminal plasma miR-192a appears to be a potential marker for successfully indicating spermatozoa in the ejaculate following microsurgical varicocelectomy in men with nonobstructive azoospermia and varicoceles. Seminal plasma miR-192a may be a useful clinical marker for prescreening to determine which patients with nonobstructive azoospermia and varicoceles would benefit from varicocelectomy. 展开更多
关键词 mir-192a nonobstructive azoospermia seminal plasma VARICOCELECTOMY
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Enhanced Expression of miR-425 Promotes Esophageal Squamous Cell Carcinoma Tumorigenesis by Targeting SMAD2 被引量:11
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作者 Lingyan Liu Zitong Zhao +3 位作者 Wei Zhou Xinyi Fan Qimin Zhan Yongmei Song 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2015年第11期601-611,共11页
Esophageal squamous cell carcinoma (ESCC) is one of the most common and deadly cancers in the world, Currently, clinical therapy of ESCC remains limited and the five-year survival rate is poor. The function of miR-4... Esophageal squamous cell carcinoma (ESCC) is one of the most common and deadly cancers in the world, Currently, clinical therapy of ESCC remains limited and the five-year survival rate is poor. The function of miR-425 has been reported in multiple human cancers. However. the tumorigenic role and clinical significance of miR-425 in ESCC remains unclear. We found that enhanced expression of miR- 425 in ESCC cell lines not only promoted cell proliferation and colony formation, but also increased cellular metastasis. Furthermore, we revealed the mechanism that miR-425 inhibited the expression of SMAD2 by targeting the second binding site in the 3'-untranslated region (3'-UTR) in ESCC. This mode of action influenced not only SMAD2 rnRNA expression but also protein expression. In addition, we detected the expression of miR-425 in ESCC tissues and plasma. Moreover, we analyzed the relationship between miR-425 expression and SMAD2 mRNA expression. We found that miR-425 was overexpressed in ESCC tissues and the plasma relative to adjacent normal tissues and plasma of healthy individuals. Furthermore, there was a negative correlation between miR-425 expression and SMAD2, Taken together, our results show that miR-425 functions as an oncogene by targeting the 3'-UTR of SMAD2 and indicate the potential utility of plasma miR-425 as a novel biomarker for ESCC diagnosis. 展开更多
关键词 Esophageal squamous cell carcinoma mir-425 plasma miRNA SMAD2
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miR-503-3p promotes epithelialemesenchymal transition in breast cancer by directly targeting SMAD2 and E-cadherin 被引量:15
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作者 Zitong Zhao Xinyi Fan +4 位作者 Lanfang Jiang Zhongqiu Xu Liyan Xue Qimin Zhan Yongmei Song 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2017年第2期75-84,共10页
Although progress in clinical and basic research has significantly increased our understanding of breast cancer, little is known about the molecular mechanism underlying breast cancer metastasis. Identification of eff... Although progress in clinical and basic research has significantly increased our understanding of breast cancer, little is known about the molecular mechanism underlying breast cancer metastasis. Identification of effective therapeutic targets to prevent breast cancer metastasis is urgently needed. The function of mi R-503-3p has been investigated in other cancers, but its role in breast cancer remains undefined.Here, we found that mi R-503-3p was overexpressed in breast cancer tissue and plasma compared with adjacent normal breast tissue and with plasma from healthy individuals. Moreover, we identified mi R-503-3p to be an oncogene of breast cancer cell proliferation, migration and invasion. Upregulation of mi R-503-3p in breast cancer cells inhibited expression of epithelialemesenchymal transition(EMT)-related protein SMAD2 and the epithelial marker protein E-cadherin by directly binding to their m RNA30 untranslated region, whereas increased expression of mesenchymal marker proteins, including vimentin and N-cadherin. Taken together, our findings support a critical role for mi R-503-3p in induction of breast cancer EMT and suggest that plasma mi R-503-3p may be a useful diagnostic biomarker for breast cancer. 展开更多
关键词 Breast cancer mir-503-3p plasma miRNA SMAD2 E-cadherin
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