Objective: To explore the characteristics of crisis state at erythrocytic stage of Plasmodium berghei chloroquine-resistant (RC) strain. Methods: Agarose electrophoresis, optical and transmission electron microscopes ...Objective: To explore the characteristics of crisis state at erythrocytic stage of Plasmodium berghei chloroquine-resistant (RC) strain. Methods: Agarose electrophoresis, optical and transmission electron microscopes were used. Patterns of genomic DNA structures and ultra-structures of the erythrocytic parasites were observed in ICA mice (infected with the RC strain) during rising and declining of parasitemia. Results: During the declining parasitemia, the erythrocytic stage parasites of the RC strain showed round or oval appearance with intact plasma membrane and shrank nuclei with no metabolic window, mitochondria or other membranaceous structures. Their DNA electrophoretogram revealed a ladder pattern which evidently differed from the parasites of the RC strain in the rising parasitemia and the chloroquine-sensitive (N) strain.Conclusion: The crisis state of the erythrocytic stage parasites of the P. berghei chloroquine-resistant (RC)strain is characterized by apoptosis.展开更多
目的:研究疟原虫抗青蒿酯钠的产生机理。方法:分别采用感染抗青篙酯钠伯氏疟原虫株小鼠血清(SM SA-R)、感染青蒿酯钠敏感株伯氏疟原虫小鼠血清(SM SA-S)和无原虫感染的正常小鼠血清(SM)与青蒿酯钠(SA)水溶液的混合物治疗感染正常伯氏疟...目的:研究疟原虫抗青蒿酯钠的产生机理。方法:分别采用感染抗青篙酯钠伯氏疟原虫株小鼠血清(SM SA-R)、感染青蒿酯钠敏感株伯氏疟原虫小鼠血清(SM SA-S)和无原虫感染的正常小鼠血清(SM)与青蒿酯钠(SA)水溶液的混合物治疗感染正常伯氏疟原虫小鼠,于给药后3天(d3)、4天(d4)和5天(d5)涂片检查其感染率。结果:SM SA-R 加 SA 组、SMSA-S 加 SA 组和 SM 加 SA 组的原虫感染率分别为:d3:(16.8±1.7)%、(10.3±2.5)%、(9.0±2.0)%,d4:(28.6±5.0)%、(20.9±5.2)%、(17.6±4.4)%,d5:(39.2±8.5)%、(27.0±7.1)%、(23.1±5.4)%。SM SA-R 加 SA 组与其他两组原虫感染率比较,差异显著(P<0.01)。结论:SM SA-R 对 SA 抗疟药药效具有拮抗作用。提示 SA-R 疟原虫可能产生了某种“特异性抗药物质”。展开更多
基金Supported by the National Natural Sciences Foundation of China (No. 39880041)
文摘Objective: To explore the characteristics of crisis state at erythrocytic stage of Plasmodium berghei chloroquine-resistant (RC) strain. Methods: Agarose electrophoresis, optical and transmission electron microscopes were used. Patterns of genomic DNA structures and ultra-structures of the erythrocytic parasites were observed in ICA mice (infected with the RC strain) during rising and declining of parasitemia. Results: During the declining parasitemia, the erythrocytic stage parasites of the RC strain showed round or oval appearance with intact plasma membrane and shrank nuclei with no metabolic window, mitochondria or other membranaceous structures. Their DNA electrophoretogram revealed a ladder pattern which evidently differed from the parasites of the RC strain in the rising parasitemia and the chloroquine-sensitive (N) strain.Conclusion: The crisis state of the erythrocytic stage parasites of the P. berghei chloroquine-resistant (RC)strain is characterized by apoptosis.
文摘目的:研究疟原虫抗青蒿酯钠的产生机理。方法:分别采用感染抗青篙酯钠伯氏疟原虫株小鼠血清(SM SA-R)、感染青蒿酯钠敏感株伯氏疟原虫小鼠血清(SM SA-S)和无原虫感染的正常小鼠血清(SM)与青蒿酯钠(SA)水溶液的混合物治疗感染正常伯氏疟原虫小鼠,于给药后3天(d3)、4天(d4)和5天(d5)涂片检查其感染率。结果:SM SA-R 加 SA 组、SMSA-S 加 SA 组和 SM 加 SA 组的原虫感染率分别为:d3:(16.8±1.7)%、(10.3±2.5)%、(9.0±2.0)%,d4:(28.6±5.0)%、(20.9±5.2)%、(17.6±4.4)%,d5:(39.2±8.5)%、(27.0±7.1)%、(23.1±5.4)%。SM SA-R 加 SA 组与其他两组原虫感染率比较,差异显著(P<0.01)。结论:SM SA-R 对 SA 抗疟药药效具有拮抗作用。提示 SA-R 疟原虫可能产生了某种“特异性抗药物质”。
