期刊文献+
共找到14篇文章
< 1 >
每页显示 20 50 100
解偶联剂CCCP调控海洋绿藻Platymonas subcordiformis的光照产氢特征
1
作者 冉春秋 乌云娜 +2 位作者 王冰 张凤杰 江姗 《太阳能学报》 EI CAS CSCD 北大核心 2009年第10期1341-1346,共6页
研究了不同浓度解偶联剂CCCP对海水绿藻Platymonas subcordiformis光照产氢的影响。研究结果显示:厌氧暗诱导后,PSII光化学活性、光合放氧能力和光照产氢能力与CCCP浓度密切相关;藻液中CCCP浓度超过4μmol·L^(-1)时,藻细胞PSII光... 研究了不同浓度解偶联剂CCCP对海水绿藻Platymonas subcordiformis光照产氢的影响。研究结果显示:厌氧暗诱导后,PSII光化学活性、光合放氧能力和光照产氢能力与CCCP浓度密切相关;藻液中CCCP浓度超过4μmol·L^(-1)时,藻细胞PSII光化学活性被持续抑制,光合放氧能力明显降低,密闭藻液体系能够保持厌氧状态,进行光照产氢12h以上;光照产氢所需电子的90%来自PSII光解水,10%来自内源底物代谢;随着CCCP浓度增加,其对氢酶活性的抑制作用增强。同时,研究了10μmol/L CCCP对不同pH藻液直接光照产氢的影响。 展开更多
关键词 platymonas subcordiformis 光照 CCCP 产氢 氧抑制 光化学活性
下载PDF
Effects of strontium-induced stress on marine microalgae Platymonas subcordiformis (Chlorophyta: Volvocales) 被引量:8
2
作者 李梅 谢玺韬 +1 位作者 薛任皓 刘志礼 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2006年第2期154-160,共7页
Strontium-induced stress in growth and chlorophyll contents of Platymonas subcordiformis was investigated under laboratory condition. The results showed that strontium exposure had little influ- ences in general on gr... Strontium-induced stress in growth and chlorophyll contents of Platymonas subcordiformis was investigated under laboratory condition. The results showed that strontium exposure had little influ- ences in general on growth and chlorophyll contents of the algae except for very high Sr concentrations. The maximum biosorption capacity of strontium ranged from 69.62 to 269.18 mg Sr2+/g dry weight. The algal biomass exhibited high uptake capacity of strontium. Total superoxide dismutase (SOD) activity and the content of lipid peroxidation products malondialdehyde (MDA) were significantly different in differ- ent treatments. SOD activity reached the highest level at 0.09 mmol/L that was about 55.8 % higher than that in the control. The MDA content increased significantly at 0.36 mmol/L, which was 2.15 times higher than that in the control, indicating a state of oxidative stress. With the increase of strontium concentration, the amount of fatty acids decreased. 展开更多
关键词 platymonas subcordiformis strontium stress BIOSORPTION
下载PDF
Genetic transformation of Platymonas (Tetraselmis) subcordiformis (Prasinophyceae, Chlorophyta) using particle bombardment and glass-bead agitation 被引量:1
3
作者 崔玉琳 姜鹏 +4 位作者 王金锋 李富超 陈英杰 郑国庭 秦松 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2012年第3期471-475,共5页
Platymonas (Tetraselmis) subcordiformis is a unicellular marine green alga. It was found to be very sensitive to the herbicide Basta through a sensitivity test indicating it could be employed as a selective agent. The... Platymonas (Tetraselmis) subcordiformis is a unicellular marine green alga. It was found to be very sensitive to the herbicide Basta through a sensitivity test indicating it could be employed as a selective agent. The bar gene is a practicable and selectable marker gene. The vector containing the expression cassette of the bar gene was transferred to P. subcordiformis by both particle bombardment and glass-bead agitation and transformants were then selected using Basta. Finally, Southern blotting analysis indicated that the bar gene had been successfully integrated into the nuclear genome of P. subcordiformis using both of the transgenic techniques, with the transformation efficiency of the glass-bead method being slightly higher than that of particle bombardment. This is the first report on stable transformation of P. subcordiformis, and will improve fundamental research and enlarge application of this alga. 展开更多
关键词 bar glass-bead agitation nuclear transformation particle bombardment platymonas subcordiformis
下载PDF
The effects of immobilization on physiology and ultrastructure of unicellular alga Platymonas subcordiformis
4
作者 Zhang Xuecheng Wu Xiaonan and Li Yonghong(College of Marine Life Sciences, Ocean University of Qingdao, Qingdao 266003, China Institute of Oceanology, Academia Sinica, Qingdao 266071, China) 《Acta Oceanologica Sinica》 SCIE CAS CSCD 1995年第2期269-275,共7页
In the present paper, the unicellular green alga Platymonas subcordiformis was entrapped in 2% (W/V) alginate, treated with CaCl2 at different concentrations (0. 5, 0.75, 1. 0, 1. 5 and 2. 0 mol/dm3), cultured at 15℃... In the present paper, the unicellular green alga Platymonas subcordiformis was entrapped in 2% (W/V) alginate, treated with CaCl2 at different concentrations (0. 5, 0.75, 1. 0, 1. 5 and 2. 0 mol/dm3), cultured at 15℃ and 25℃. The growth rate and morphology were studied and photosynthesis and respiration rates were measured. The optimum conditions of immobilized culture is in 0. 5 mol/dm3 of CaCl2, at 25℃. Photosynthesis rate of immobilized cells is higher than free cultivated cells, and that at 15℃ than at 25℃. Under scanning electronic microscope, it was shown that the immobilized cells had larger size and rougher surface. The relationship between growth of immobilized cells and abiotic factors and the prospects of applying immobilized cultivation are also discussed. 展开更多
关键词 platymonas subcordiformis IMMOBILIZATION photosynthesis RESPIRATION ULTRASTRUCTURE
下载PDF
孔石莼与2种海洋微藻的胞外滤液交叉培养研究 被引量:8
5
作者 张培玉 蔡恒江 +3 位作者 肖慧 于娟 冯蕾 唐学玺 《海洋科学》 CAS CSCD 北大核心 2006年第5期1-4,共4页
在实验室条件下,采用海洋大型海藻石莼(Ulva pertusaKjellm.)和海洋微藻青岛大扁藻(Platymonas helgolandicaKylin var.tsingtaoensis)、亚历山大藻(Alexandrium tamarense(Lebour)Balech)的滤液做了交叉培养研究,初步探索藻间的克生机... 在实验室条件下,采用海洋大型海藻石莼(Ulva pertusaKjellm.)和海洋微藻青岛大扁藻(Platymonas helgolandicaKylin var.tsingtaoensis)、亚历山大藻(Alexandrium tamarense(Lebour)Balech)的滤液做了交叉培养研究,初步探索藻间的克生机制。试验结果表明,不同温度处理下,2种微藻滤液对石莼生长的影响均有显著的差异。不同温度处理的石莼滤液对青岛大扁藻生长的影响差异显著,而对亚历山大藻生长的影响差异不显著。可以推测:石莼对亚历山大藻的抑制作用很可能是通过细胞的直接接触来完成的;而石莼对青岛大扁藻的抑制作用和两种微藻对石莼的抑制作用主要是通过分泌胞外产物(Extracellular products,ECP)来实现的,至于其它的方式和途径有待于进一步研究。 展开更多
关键词 石莼(Ulva pertusa Kjellm.) 青岛大扁藻(platymonas helgolandica KYLIN var.tsingtaoensis) 亚历山大藻(Alexandrium tamarense(Lebour)Balech) 胞外产物 竞争
下载PDF
混合氮源对扁藻与金藻共培养和单种培养生长的影响 被引量:8
6
作者 朱艺峰 林霞 +1 位作者 朱鹏 李洋 《海洋科学》 CAS CSCD 北大核心 2006年第8期34-40,共7页
采用均匀设计,研究了硝酸钠(NO3-N)、尿素(NH2-N)和硫酸铵(NH4-N)3种氮源对亚心形扁藻(Platymonas subcordiformis)与球等鞭金藻(Isochrysis galbana)共培养和单种培养生长的影响。结果表明,不同氮源对扁藻与金藻在不同培养方式下的生... 采用均匀设计,研究了硝酸钠(NO3-N)、尿素(NH2-N)和硫酸铵(NH4-N)3种氮源对亚心形扁藻(Platymonas subcordiformis)与球等鞭金藻(Isochrysis galbana)共培养和单种培养生长的影响。结果表明,不同氮源对扁藻与金藻在不同培养方式下的生长均存在显著差异(P<0.05)。共培养时,硝酸钠质量浓度对扁藻和金藻的总细胞密度(Yt)和最终细胞密度的体积比(Yr)没有影响,尿素质量浓度对Yt有正调节作用,而硫酸铵质量浓度对Yt有负调节作用、对Yr有正调节作用。当培养液中NH2-N质量浓度为21.01 mg/L,扁藻和金藻接种体积比(Xv)为14.15时,Yt最大(1 508.9×104个/mL);当NH4-N质量浓度、Xv分别为21.00 mg/L和14.15时,Yr最大,为87.46。单种培养金藻时,硝酸钠略优于尿素,2种氮源对其生长均有正调节作用,而硫酸铵起负调节作用。当培养液中加21.01 mg/L的NO3-N和NH2-N时,金藻的细胞密度达到最大值(862.6×104个/mL);不同氮源对扁藻生长的调节作用与金藻相反,当扁藻接种密度为4.6×104个/mL,培养液中NH4-N质量浓度为21.00mg/L时,扁藻细胞密度达到102.2×104个/mL,其值最大。 展开更多
关键词 氮源 亚心形扁藻(platymonas subcordiformis) 球等鞭金藻(Isochrysis galbana) 共培养 单种培养 生长
下载PDF
亚心型扁藻叶绿体psbA基因的克隆及序列分析 被引量:5
7
作者 侯士昌 崔玉琳 +2 位作者 温少红 唐志红 秦松 《生物学杂志》 CAS CSCD 2014年第1期1-4,14,共5页
psbA基因是叶绿体基因组中一个重要的光调控基因,编码光和系统Ⅱ反应中心的D1蛋白。根据叶绿体基因组序列高度保守的特性,利用莱茵衣藻(Chlamydomonas reinhardtii)psbA基因的保守序列(基因登录号:HQ667991.1)设计引物,采用PCR步移的方... psbA基因是叶绿体基因组中一个重要的光调控基因,编码光和系统Ⅱ反应中心的D1蛋白。根据叶绿体基因组序列高度保守的特性,利用莱茵衣藻(Chlamydomonas reinhardtii)psbA基因的保守序列(基因登录号:HQ667991.1)设计引物,采用PCR步移的方法从亚心型扁藻(Platymonas subcordiformis)基因组DNA中克隆到psbA基因全长(基因登录号:KF528742)。序列分析表明,亚心型扁藻psbA基因全长1939 bp,编码区长度为1062 bp,推导编码353个氨基酸,包括4个赖氨酸残基。有效密码子数显示psbA基因具有明显的密码子偏好性,并且偏好使用以A/T结尾的密码子。相对同义密码子使用度表明25个密码子在编码使用时具有偏好性,其中20个密码子以A/T碱基结尾,占到80%。其终止密码子使用了TAG。 展开更多
关键词 亚心型扁藻(platymonas subcordiformis) PCR步移 PSBA基因 序列分析
下载PDF
模拟电厂温排水温升和游离余氯对亚心形扁藻生长影响的研究 被引量:2
8
作者 戴雅奇 仇建标 +4 位作者 潘利平 彭欣 李彬彬 黄莹莹 陈雪初 《海洋科学》 CAS CSCD 北大核心 2018年第2期134-140,共7页
以亚心形扁藻(Platymonas subcordiformis)为受试藻种,模拟温升和游离余氯条件,研究浮游植物对核电厂温排水的生态响应。结果表明,春秋季温升对其生长影响较小,而在夏季,其生长受到显著抑制。亚心形扁藻对游离余氯非常敏感,0.1 mg/L游... 以亚心形扁藻(Platymonas subcordiformis)为受试藻种,模拟温升和游离余氯条件,研究浮游植物对核电厂温排水的生态响应。结果表明,春秋季温升对其生长影响较小,而在夏季,其生长受到显著抑制。亚心形扁藻对游离余氯非常敏感,0.1 mg/L游离余氯既能抑制其生长,抑制作用随着游离余氯浓度的升高而增强。温升会加剧游离余氯的抑制作用,4℃温升和0.1 mg/L游离余氯联合作用对亚心形扁藻的抑制效果明显增加。由于温升和游离余氯的联合作用,即使在离排口较远的水域,仍然会导致亚心形扁藻数量减少。 展开更多
关键词 温排水 温升 游离余氯 亚心形扁藻(platymonas subcordiformis)
下载PDF
无硫培养对扁藻的氢代谢及其吸收光谱的影响 被引量:1
9
作者 管英富 邓麦村 +2 位作者 金美芳 虞星炬 张卫 《植物生理学通讯》 CSCD 北大核心 2003年第6期605-608,共4页
扁藻在无硫海水培养基中的放氢能力比在天然海水中的高 13倍。在此过程中 。
关键词 platymonas subcordiformis 氢代谢 无硫培养 吸收光谱
下载PDF
富勒醇减轻蒽对亚心形扁藻伤害的作用研究
10
作者 黄健 王俊 +2 位作者 曲爱敏 陈滇宝 唐学玺 《海洋科学》 CAS CSCD 北大核心 2004年第9期78-80,共3页
采用新型抗氧化剂———富勒醇((C60(OH)n),探讨了其减轻蒽对亚心形扁藻(Plattymonassubcordiformis)伤害的作用。亚心形扁藻在蒽的半致死浓度0.06mg/L胁迫下分别加入n=14,浓度为2.29×103mg/L的C60(OH)n20μL和n≥20浓度同样为为2.... 采用新型抗氧化剂———富勒醇((C60(OH)n),探讨了其减轻蒽对亚心形扁藻(Plattymonassubcordiformis)伤害的作用。亚心形扁藻在蒽的半致死浓度0.06mg/L胁迫下分别加入n=14,浓度为2.29×103mg/L的C60(OH)n20μL和n≥20浓度同样为为2.29×103mg/L的C60(OH)n200μL后,分别测定了对照组和胁迫组在0、12、24、36、、8、60和72h的相对增长率K 并作图。结果分析表明富勒醇(C60(OH)n)有减轻亚心形扁藻蒽伤害的作用 。 展开更多
关键词 亚心形扁藻(platymonas subcordiformis) 富勒醇
下载PDF
两种光强条件下亚心形扁藻各生长阶段的产氢能力 被引量:3
11
作者 刘润国 虞星炬 +1 位作者 金美芳 张卫 《海洋科学》 CAS CSCD 北大核心 2008年第3期1-5,共5页
海洋亚心形扁藻(Platymonas subcordiformis)在光照生长后,经过暗培养诱导其可逆产氢酶,然后转移到光照下可产生氢气。在300 mL产氢反应器中对海洋亚心形扁藻产氢情况的考察表明,不同的光强条件可导致扁藻产生不同的生长曲线,而在不同... 海洋亚心形扁藻(Platymonas subcordiformis)在光照生长后,经过暗培养诱导其可逆产氢酶,然后转移到光照下可产生氢气。在300 mL产氢反应器中对海洋亚心形扁藻产氢情况的考察表明,不同的光强条件可导致扁藻产生不同的生长曲线,而在不同的培养阶段,扁藻的产氢能力也有很大的差别。其他培养条件相同,高光照强度(E=13000 lx)条件下,扁藻生长较快,而且可以获得更大的产氢能力,300 mL密度为3×106个/mL、叶绿素质量浓度为5.8 mg/L的藻液最大产氢浓度可达17.5%,比低光照强度(E=5000 lx)时提高了32%,叶绿素含量降低了61%。根据产氢前后以及暗诱导后扁藻生理状态的考察结果,可以推测出是不同生长阶段扁藻的生理状态差异导致了其产氢能力的变化。 展开更多
关键词 亚心形扁藻(platymonas subcordiformis) 生长阶段 可逆产氢酶 氢能
下载PDF
Studies on the Effects on Growth and Antioxidant Responses of Two Marine Microalgal Species to Uniconazole 被引量:3
12
作者 MEI Xueqiao ZHENG Kang +1 位作者 WANG Lingdong LI Yantuan 《Journal of Ocean University of China》 SCIE CAS 2014年第5期877-882,共6页
Uniconazole, as a plant growth retardant, can enhance stress tolerance in plants, possibly because of improved antioxidation defense mechanisms with higher activities of superoxide dismutase(SOD) and peroxidase(POD) e... Uniconazole, as a plant growth retardant, can enhance stress tolerance in plants, possibly because of improved antioxidation defense mechanisms with higher activities of superoxide dismutase(SOD) and peroxidase(POD) enzymes that retard lipid peroxidation and membrane deterioration. These years much attention has been focused on the responses of antioxidant system in plants to uniconazole stress, but such studies on aquatic organism are very few. Moreover, no information is available on growth and antioxidant response in marine microalgae to uniconazole. In this paper, the growth and antioxidant responses of two marine microalgal species, Platymonas helgolandica and Pavlova viridis, at six uniconazole concentrations(0-15 mg L-1) were investigated. The results demonstrated that 3 mg L-1 uniconazole could increase significantly chlorophyll a and carbohydrate contents of P. helgolandica(P < 0.05). Higher concentrations(≥12 mg L-1) of uniconazole could inhibit significantly the growth, dry weight, chlorophyll-a and carbohydrate contents of P. helgolandica and P. viridis(P < 0.05). Uniconazole caused a significant increase in lipid peroxidation production(MDA) at higher concentrations(≥ 9 mg L-1). The activities of antioxidant enzymes, superoxide dismutase(SOD) and catalase(CAT) were enhanced remarkably at low concentrations of uniconazole. However, significant reduction of SOD and CAT activities was observed at higher concentrations of uniconazole. 展开更多
关键词 catalase(CAT) lipid peroxidation platymonas helgolandica Pavlova viridis superoxide dismutase(SOD) UNICONAZOLE
下载PDF
A Comparative Study of the Sensitivity of F_v/F_m to Phosphorus Limitation on Four Marine Algae 被引量:1
13
作者 QI Hongju WANG Jiangtao WANG Zhaoyu 《Journal of Ocean University of China》 SCIE CAS 2013年第1期77-84,共8页
We studied the effects of nitrogen (N) and phosphorus (P) supply on Fv/Fm (maximal quantum yield of photosystem Ⅱ) in the algae Chaetoceros debilis, Dicrateria inornata, Platymonas subcordiformis and Heterosigm... We studied the effects of nitrogen (N) and phosphorus (P) supply on Fv/Fm (maximal quantum yield of photosystem Ⅱ) in the algae Chaetoceros debilis, Dicrateria inornata, Platymonas subcordiformis and Heterosigma akashiwo to determine the sensitivity of Fv/Fm to P-limitation of the four species. Obvious decrease of Fv/Fm value was monitored in periods of P-depletion, the decrease showing different magnitudes among algal species. A more clear decrease of Fv/Fm in Platymonas subcordiformis and Heterosigma akashiwo was observed, compared with that in Chaetoceros debilis and Dicrateria inornata. After the re-supply of P, the index Fv/Fm of the four species all recovered quickly in 24h. Cell division was maintained and chlorophyll content continued to increase until the end of the experiment in Platymonas subcordiformis and Heterosigma akashiwo, while in Chaetoceros debilis and Dicrateria inornata, the division stopped in the later period when the growth of organisms came into the stationary phase. The most obvious respond of chlorophyll content to P-additions was observed in Heterosigma akashiwo culture in 24 h. The sensitivity of Fv/Fm to nutrient limitations is different among algal species, and much should be done to improve the application of this index. 展开更多
关键词 Chaetoceros debilis Dicrateria inornata platymonas subcordiformis Heterosigma akashiwo P limitation P depletion Fv/Fm ratio
下载PDF
Production of dimethylsulfide and acrylic acid from dimethylsulfoniopropionate during growth of three marine microalgae 被引量:1
14
作者 刘春颖 高彩霞 +4 位作者 张海波 陈烁 邓平 岳新安 郭肖依 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2014年第6期1270-1279,共10页
We measured the concentrations of dimethylsulfide(DMS),acrylic acid(AA),and dimethylsulfoniopropionate(DMSP) during growth of three microalgae:Prorocentrum micans,Gephyrocapsa oceanica,and Platymonas subcordiformis.Th... We measured the concentrations of dimethylsulfide(DMS),acrylic acid(AA),and dimethylsulfoniopropionate(DMSP) during growth of three microalgae:Prorocentrum micans,Gephyrocapsa oceanica,and Platymonas subcordiformis.The DMSP,AA,and DMS concentrations in culture media varied significantly among algal growth stages,with the highest concentrations in the late stationary growth stage or the senescent stage.In the stationary growth stage,the average DMSP concentration per cell in P.micans(0.066 5 pmol/cell) was 1.3 times that in G.oceanica(0.049 5 pmol/cell) and 20.2 times that in P.subcordiformis(0.003 29 pmol/cell).The average concentrations of AA were0.044 6,0.026 9,and 0.003 05 pmol/cell in P.micans,G.oceanica,and P.subcordiformis,respectively,higher than the concentrations of DMS(0.272,0.497,and 0.086 2 fmol/cell,respectively).There were significant positive correlations between cell density and AA,DMSP,and DMS concentrations.The ratios of DMS/AA and AA/(DMSP+AA) in the three algae differed significantly over the growth cycle.In all three microalgae,the DMS/AA ratios were less than 25%during the growth period,suggesting that the enzymatic cleavage pathway,which generates DMS,was not the main DMSP degradation pathway.The changes in the DMS/AA ratio indicated that there was a higher rate of enzymatic breakdown of DMSP in the early growth period and a lower rate during senescence.In all three microalgae,the AA/(DMSP+AA) ratio(degradation ratio of DMSP) decreased during the exponential growth phase,and then increased.The variations in these ratios can approximately indicate the cleavage mechanism of DMSP at different stages of algal growth. 展开更多
关键词 Prorocentrum micans' Gephyrocapsa oceanica platymonas subcordiformis' dimethylsulfide(DMS) acrylic acid (AA) dimethysulfoniopropionate (DMSP)
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部