BACKGROUND For the first time,we investigated the oncological role of plexin domain-containing 1(PLXDC1),also known as tumor endothelial marker 7(TEM7),in hepatocellular carcinoma(HCC).AIM To investigate the oncologic...BACKGROUND For the first time,we investigated the oncological role of plexin domain-containing 1(PLXDC1),also known as tumor endothelial marker 7(TEM7),in hepatocellular carcinoma(HCC).AIM To investigate the oncological profile of PLXDC1 in HCC.METHODS Based on The Cancer Genome Atlas database,we analyzed the expression of PLXDC1 in HCC.Using immunohistochemistry,quantitative real-time polymerase chain reaction(qRT-PCR),and Western blotting,we validated our results.The prognostic value of PLXDC1 in HCC was analyzed by assessing its correlation with clinicopathological features,such as patient survival,methylation level,tumor immune microenvironment features,and immune cell surface checkpoint expression.Finally,to assess the immune evasion potential of PLXDC1 in HCC,we used the tumor immune dysfunction and exclusion(TIDE)website and immunohistochemical staining assays.RESULTS Based on immunohistochemistry,qRT-PCR,and Western blot assays,overexpression of PLXDC1 in HCC was associated with poor prognosis.Univariate and multivariate Cox analyses indicated that PLXDC1 might be an independent prognostic factor.In HCC patients with high methylation levels,the prognosis was worse than in patients with low methylation levels.Pathway enrichment analysis of HCC tissues indicated that genes upregulated in the high-PLXDC1 subgroup were enriched in mesenchymal and immune activation signaling,and TIDE assessment showed that the risk of immune evasion was significantly higher in the high-PLXDC1 subgroup compared to the low-PLXDC1 subgroup.The high-risk group had a significantly lower immune evasion rate as well as a poor prognosis,and PLXDC1-related risk scores were also associated with a poor prognosis.CONCLUSION As a result of this study analyzing PLXDC1 from multiple biological perspectives,it was revealed that it is a biomarker of poor prognosis for HCC patients,and that it plays a role in determining immune evasion status.展开更多
目的:基于尿蛋白组学技术,筛选IgA肾病(IgAN)患者表达差异的蛋白,为探索IgAN发病机制和发现潜在生物标志物提供新的线索。方法:分析IgAN、膜性肾病及健康对照者的尿液蛋白组学数据。将膜性肾病患者和健康者作为对照,筛选出IgAN中差异表...目的:基于尿蛋白组学技术,筛选IgA肾病(IgAN)患者表达差异的蛋白,为探索IgAN发病机制和发现潜在生物标志物提供新的线索。方法:分析IgAN、膜性肾病及健康对照者的尿液蛋白组学数据。将膜性肾病患者和健康者作为对照,筛选出IgAN中差异表达的蛋白。筛选出蛋白Plexin-B2与临床病理指标进行相关性分析。采用酶联免疫吸附试验(ELISA)对血、尿中Plexin-B2及其配体血管生成素(ANG)表达情况进行检测验证并采用免疫组化检测其在肾脏的表达。结果:与健康对照组相比,质谱法检测的IgAN患者尿液Plexin-B2(uPlexin-B2)表达明显增加,且与总胆固醇(r=0.34,P=0.02)及低密度脂蛋白(r=0.43,P=0.01)呈正相关。uPlexin-B2与节段性肾小球硬化(r=-0.28,P=0.04)呈负相关。ELISA发现IgAN患者uPlexin-B2有升高的趋势。IgAN患者血Ang表达水平明显高于健康对照组(61.97 vs 6.30,P<0.001)。血ANG与肾毛细血管内增殖呈负相关(r=-0.40,P=0.04),与节段性硬化个数呈负相关(r=-0.44,P=0.04)。uPlexin-B2与肾脏球性硬化个数呈负相关(r=-0.95,P=0.01)。Plexin-B2及ANG均在IgAN患者肾小管中高表达。结论:IgAN患者uPlexin-B2升高。Plexin-B2及ANG与IgAN中肾小球硬化显著相关。ANG在IgAN患者血液中明显升高。本研究可能为发现IgAN的潜在生物标志物提供线索,为探索肾脏病理发病机制提供新思路。展开更多
AIM:To investigate the protein and mRNA expression of semaphorin 5A and its receptor plexin B3 in gastric carcinoma and explore its role in the invasion and metastasis of gastric carcinoma.METHODS:Expression of semaph...AIM:To investigate the protein and mRNA expression of semaphorin 5A and its receptor plexin B3 in gastric carcinoma and explore its role in the invasion and metastasis of gastric carcinoma.METHODS:Expression of semaphorin 5A and its receptor plexin B3 in 48 samples of primary gastric carcinoma,its corresponding non-neoplastic mucosa,and matched regional lymph node metastasis was assayed by reverse transcription-polymerase chain reaction(RT-PCR),real-time RT-PCR and Western blotting.RESULTS:The protein and mRNA expression of semaphorin 5A and its receptor plexin B3 increased gradually in non-neoplastic mucosa,primary gastric carcinoma and lymph node metastasis(P<0.05).Moreover,the expression of semaphorin 5A was closely correlated with that of plexin B3.CONCLUSION:Semaphorin 5A and its receptor plexin B3 play an important role in the invasion and metastasis of gastric carcinoma.展开更多
Plexins and semaphorins are a large family of proteins that are involved in cell movement and response.The importance of plexins and semaphorins has been emphasized by their discovery in many organ systems including t...Plexins and semaphorins are a large family of proteins that are involved in cell movement and response.The importance of plexins and semaphorins has been emphasized by their discovery in many organ systems including the nervous(Nkyimbeng-Takwi and Chapoval,2011;McCormick and Leipzig,2012;Yaron and Sprinzak,2012),epithelial(Miao et al.,1999;Fujii et al.,2002),and immune systems(Takamatsu and Kumanogoh,2012)as well as diverse cell processes including angiogenesis(Serini et al.,2009;Sakurai et al.,2012),embryogenesis(Perala et al.,2012),and cancer(Potiron et al.,2009;Micucci et al.,2010).Plexins and semaphorins are transmembrane proteins that share a conserved extracellular semaphorin do-main(Hota and Buck,2012).The plexins and sema-phorins are divided into four and eight subfamilies respectively based on their structural homology.Semaphorins are relatively small proteins containing the extracellular semaphorin domain and short intra-cellular tails.Plexins contain the semaphorin domain and long intracellular tails(Hota and Buck,2012).The majority of plexin and semaphorin research has fo-cused on the nervous system,particularly the devel-oping nervous system,where these proteins are found to mediate many common neuronal cell processes including cell movement,cytoskeletal rearrangement,and signal transduction(Choi et al.,2008;Takamatsu et al.,2010).Their roles in the immune system are the focus of this review.展开更多
基金Supported by the Anhui Provincial Health Scientific Research Project Provincial Financial Support Key Project,No.AHWJ2023A10110College Teaching Quality Engineering Project of Anhui Educational Committee,No.2021jyxm0954College Student Innovation Training Program of Bengbu Medical College,No.Byycxz22110.
文摘BACKGROUND For the first time,we investigated the oncological role of plexin domain-containing 1(PLXDC1),also known as tumor endothelial marker 7(TEM7),in hepatocellular carcinoma(HCC).AIM To investigate the oncological profile of PLXDC1 in HCC.METHODS Based on The Cancer Genome Atlas database,we analyzed the expression of PLXDC1 in HCC.Using immunohistochemistry,quantitative real-time polymerase chain reaction(qRT-PCR),and Western blotting,we validated our results.The prognostic value of PLXDC1 in HCC was analyzed by assessing its correlation with clinicopathological features,such as patient survival,methylation level,tumor immune microenvironment features,and immune cell surface checkpoint expression.Finally,to assess the immune evasion potential of PLXDC1 in HCC,we used the tumor immune dysfunction and exclusion(TIDE)website and immunohistochemical staining assays.RESULTS Based on immunohistochemistry,qRT-PCR,and Western blot assays,overexpression of PLXDC1 in HCC was associated with poor prognosis.Univariate and multivariate Cox analyses indicated that PLXDC1 might be an independent prognostic factor.In HCC patients with high methylation levels,the prognosis was worse than in patients with low methylation levels.Pathway enrichment analysis of HCC tissues indicated that genes upregulated in the high-PLXDC1 subgroup were enriched in mesenchymal and immune activation signaling,and TIDE assessment showed that the risk of immune evasion was significantly higher in the high-PLXDC1 subgroup compared to the low-PLXDC1 subgroup.The high-risk group had a significantly lower immune evasion rate as well as a poor prognosis,and PLXDC1-related risk scores were also associated with a poor prognosis.CONCLUSION As a result of this study analyzing PLXDC1 from multiple biological perspectives,it was revealed that it is a biomarker of poor prognosis for HCC patients,and that it plays a role in determining immune evasion status.
文摘目的:基于尿蛋白组学技术,筛选IgA肾病(IgAN)患者表达差异的蛋白,为探索IgAN发病机制和发现潜在生物标志物提供新的线索。方法:分析IgAN、膜性肾病及健康对照者的尿液蛋白组学数据。将膜性肾病患者和健康者作为对照,筛选出IgAN中差异表达的蛋白。筛选出蛋白Plexin-B2与临床病理指标进行相关性分析。采用酶联免疫吸附试验(ELISA)对血、尿中Plexin-B2及其配体血管生成素(ANG)表达情况进行检测验证并采用免疫组化检测其在肾脏的表达。结果:与健康对照组相比,质谱法检测的IgAN患者尿液Plexin-B2(uPlexin-B2)表达明显增加,且与总胆固醇(r=0.34,P=0.02)及低密度脂蛋白(r=0.43,P=0.01)呈正相关。uPlexin-B2与节段性肾小球硬化(r=-0.28,P=0.04)呈负相关。ELISA发现IgAN患者uPlexin-B2有升高的趋势。IgAN患者血Ang表达水平明显高于健康对照组(61.97 vs 6.30,P<0.001)。血ANG与肾毛细血管内增殖呈负相关(r=-0.40,P=0.04),与节段性硬化个数呈负相关(r=-0.44,P=0.04)。uPlexin-B2与肾脏球性硬化个数呈负相关(r=-0.95,P=0.01)。Plexin-B2及ANG均在IgAN患者肾小管中高表达。结论:IgAN患者uPlexin-B2升高。Plexin-B2及ANG与IgAN中肾小球硬化显著相关。ANG在IgAN患者血液中明显升高。本研究可能为发现IgAN的潜在生物标志物提供线索,为探索肾脏病理发病机制提供新思路。
文摘AIM:To investigate the protein and mRNA expression of semaphorin 5A and its receptor plexin B3 in gastric carcinoma and explore its role in the invasion and metastasis of gastric carcinoma.METHODS:Expression of semaphorin 5A and its receptor plexin B3 in 48 samples of primary gastric carcinoma,its corresponding non-neoplastic mucosa,and matched regional lymph node metastasis was assayed by reverse transcription-polymerase chain reaction(RT-PCR),real-time RT-PCR and Western blotting.RESULTS:The protein and mRNA expression of semaphorin 5A and its receptor plexin B3 increased gradually in non-neoplastic mucosa,primary gastric carcinoma and lymph node metastasis(P<0.05).Moreover,the expression of semaphorin 5A was closely correlated with that of plexin B3.CONCLUSION:Semaphorin 5A and its receptor plexin B3 play an important role in the invasion and metastasis of gastric carcinoma.
文摘Plexins and semaphorins are a large family of proteins that are involved in cell movement and response.The importance of plexins and semaphorins has been emphasized by their discovery in many organ systems including the nervous(Nkyimbeng-Takwi and Chapoval,2011;McCormick and Leipzig,2012;Yaron and Sprinzak,2012),epithelial(Miao et al.,1999;Fujii et al.,2002),and immune systems(Takamatsu and Kumanogoh,2012)as well as diverse cell processes including angiogenesis(Serini et al.,2009;Sakurai et al.,2012),embryogenesis(Perala et al.,2012),and cancer(Potiron et al.,2009;Micucci et al.,2010).Plexins and semaphorins are transmembrane proteins that share a conserved extracellular semaphorin do-main(Hota and Buck,2012).The plexins and sema-phorins are divided into four and eight subfamilies respectively based on their structural homology.Semaphorins are relatively small proteins containing the extracellular semaphorin domain and short intra-cellular tails.Plexins contain the semaphorin domain and long intracellular tails(Hota and Buck,2012).The majority of plexin and semaphorin research has fo-cused on the nervous system,particularly the devel-oping nervous system,where these proteins are found to mediate many common neuronal cell processes including cell movement,cytoskeletal rearrangement,and signal transduction(Choi et al.,2008;Takamatsu et al.,2010).Their roles in the immune system are the focus of this review.
