Knockout of C6orf120 in Rats Alleviates Concanavalin A-induced Autoimmune Hepatitis by Regulating Macrophage Polarization

Objective The effect of the functionally unknown gene C6orf120 on autoimmune hepatitis was investigated on C6orf120 knockout rats(C6orf120^(-/-))and THP-1 cells.Method Six–eight-week-old C6orf120^(-/-)and wild-type(WT)SD rats were injected with Con A(16 mg/kg),and euthanized after 24 h.The sera,livers,and spleens were collected.THP-1 cells and the recombinant protein(rC6ORF120)were used to explore the mechanism in vitro.The frequency of M1 and M2 macrophages was analyzed using flow cytometry.Western blotting and PCR were used to detect macrophage polarization-associated factors.Results C6orf120 knockout attenuated Con A-induced autoimmune hepatitis.Flow cytometry indicated that the proportion of CD68^(+)CD86^(+)M1 macrophages from the liver and spleen in the C6orf120^(-/-)rats decreased.C6orf120 knockout induced downregulation of CD86 protein and the mRNA levels of related inflammatory factors TNF-α,IL-1β,and IL-6 in the liver.C6orf120 knockout did not affect the polarization of THP-1 cells.However,rC6ORF120 promoted the THP-1 cells toward CD68^(+)CD80^(+)M1 macrophages and inhibited the CD68^(+)CD206^(+)M2 phenotype.Conclusion C6orf120 knockout alleviates Con A-induced autoimmune hepatitis by inhibiting macrophage polarization toward M1 macrophages and reducing the expression of related inflammatory factors in C6orf120^(-/-)rats.

基于^(13)C-NMR偶极去相技术的有机肥料中有机组分特征研究

【目的】在固体13C-NMR的交叉极化魔角旋转全边带抑制(cross polarization/magic angle spinning/total suppression of sidebands,CP/MAS/TOSS)技术得到的谱图中,质子碳与非质子碳的信号重叠。偶极去相(dipolar dephasing,DD)技术利用13C-1H异核耦合作用大小的差异,区分与不同质子结合的13C核基团。本研究基于DD技术探讨有机肥料中碳结构特征。【方法】供试有机肥料样品包括3种秸秆(小麦秸秆、玉米秸秆、水稻秸秆)、3种粪肥(鸡粪、牛粪、猪粪)和3种腐植酸肥(腐植酸铵、腐植酸钠、腐植酸钾)样品,利用13C-NMR谱的CP/MAS/TOSS结合DD技术,测定了不同来源的有机肥料样品图谱,比较其碳分布比例。【结果】秸秆和粪肥样品中烷氧碳组分较多,占鉴定出所有碳组分的比例分别为55%和31%~37%,非质子化的烷氧碳占比不到3%;秸秆、猪粪样品中非质子芳香碳占3%~4%,鸡粪和牛粪样品中非质子芳香碳分别占15%和11%,较秸秆和猪粪样品含有更多的非质子化芳香族化合物。粪肥和秸秆样品中甲氧基碳占比低于烷基氮。腐植酸样品以芳香碳、羧基碳和酰胺基碳为主,羧基碳和酰胺基碳占到12%~16%,总芳香族碳占48%~72%,非质子芳香碳占25%~37%,表明腐植酸芳香骨架上可能存在较多取代。【结论】基于CP/TOSS结合DD技术(偶极相移延时时间设置为40μs)分析结果发现,鸡粪和牛粪中非质子芳香碳占比高于猪粪和秸秆;腐植酸肥芳香族碳比例高于粪肥和秸秆,尤其是非质子芳香碳占比较高,表明腐植酸肥芳香化程度高于粪肥和秸秆。

准连续c切Er,Yb:YAl_(3)(BO_(3))_(4)激光器的偏振操控

通过构建谐振腔模型,分析了各向同性固体激光器中两个本征模式相干叠加后的光斑奇异特征。并且实验验证了在不使用任何特定的腔内光学偏振选择元件的情况下,在二极管泵浦的准连续c切Er,Yb:YAl_(3)(BO_(3))_(4)激光器中可以有效操控1.6μm输出激光的偏振态。实现了从部分偏振态转化成稳定的线偏振态,其线偏振方向为可切换的正交特殊情况,均具有21 dB的偏振消光比。同时通过光斑对比,验证了激光器的线偏振输出来源于两个正交本征模式的相干叠加。文中为c切Er,Yb:YAl_(3)(BO_(3))_(4)激光器线偏振光的直接输出与偏振态的调控提供了可靠的方案。

黔北一次冰雹天气过程C波段双偏振雷达资料分析

【目的】为进一步探索C波段双偏振雷达参数指标的本地化运用。【方法】利用习水C波段双偏振雷达资料、地面自动站资料、探空资料,对2022年3月16日发生在黔北的一次冰雹天气过程成因以及双偏振雷达回波特征进行分析。【结果】(1)此次强对流天气发生在500 hPa前倾槽前、低层低涡切变东南侧,中低空急流的北侧及地面低压倒槽内,同时午后升温及中高层干冷平流入侵,使得层结更趋于上干冷下暖湿的不稳定层结,冷锋前沿的地面辐合线为过程提供了触发机制。(2)过程由多单体风暴引发,最大反射率因子达65 dBz, 40 mm冰雹出现了三体散射,剖面图中出现了悬垂结构和有界弱回波区,低层观测到辐合速度对。(3)对比30 mm和40 mm的冰雹双偏振参数发现:融化层以上冰雹ZDR在-1~0.2 dB,CC值在0.90~0.99之间,KDP均为0;从融化层以下,30 mm冰雹CC值在0.70~0.90,40 mm冰雹CC值在0.80~0.97,两者有所区别,ZDR也有明显增加,在0~4 dB之间;由于融化层以下大雨滴的混合,KDP在0~3.1°·km^(-1)之间。(4)大冰雹偏振参数出现明显ZDR柱、KDP柱,CC谷,CC图中出现明显的非均匀波束充塞,ZDR和CC图中有明显的三体散射现象。【结论】该文仅是对一次多单体天气过程个例的偏振参数特征粗略分析,未来仍需分析更多个例用以完善C波段双偏振天气雷达在强对流天气中各种偏振参数的标准,为实际业务提供更精准的参考。

Determination of Structure and Polarity of Si C Single Crystal by X-Ray Diffraction Technique

Structure and polarity of the Si C single crystal have been analyzed with the four- circle X- ray diffraction method by a double- crystal diffractom eter.The hexagonal{ 10 15 } pole figure shows that this Si C sam ple has a6 H modification.The difference between the integrated intensities m easured byω scan in the triple- axis diffraction set- up finds some convincing evidence that the surface is either a Si- terminated face or C- terminated face.The experi- mental ratios of| F( 0 0 0 L) | 2 / | F( 0 0 0 L) | 2 are in good agreem entwith the calculated ones after the dispersion cor- rections to the atomic scattering factors( L=6 ,12 and18,respectively) .Thus,this m easurem ent technique is con- venient for the application of the materials with remarkable surface polarity.

Robust adaptive polarization analysis method for eliminating ground roll in 3C land seismics

To improve the data quality of converted waves, and better identify and suppress the strong ground-roll interference in three-component （3C） seismic recordings on land, we present an adaptive polarization filtering method, which can effectively separate the ground- roll interference by combining complex polarization and instantaneous polarization analysis. The ground roll noise is characterized by elliptical plane polarization, strong energy, low apparent velocity, and low frequency. After low-pass filtering of the 3C data input within a given time-window of the ground roll, the complex covariance matrix is decomposed using the sliding time window with overlapping data and length that depends on the dominant ground-roll frequency. The ground-roll model is established using the main eigenvectors, and the ground roll is detected and identified using the instantaneous polarization area attributes and average energy constraints of the ground-roll zone. Finally, the ground roll is subtracted. The threshold of the method is stable and easy to select, and offers good ground- roll detection. The method is a robust polarization filtering method. Model calculations and actual data indicate that the method can effectively identify and attenuate ground roll while preserving the effective signals.

C-C chemokine receptor type 2-overexpressing exosomes alleviated experimental post-stroke cognitive impairment by enhancing microglia/macrophage M2 polarization

BACKGROUND Human-derived mesenchymal stromal cells have been shown to improve cognitive function following experimental stroke.The activity of exosomes has been verified to be comparable to the therapeutic effects of mesenchymal stromal cells.However,the effects of exosomes derived from human umbilical cord mesenchymal stem cells(HUC-MSCs)(ExoCtrl)on post-stroke cognitive impairment(PSCI)have rarely been reported.Moreover,whether exosomes derived from C-C chemokine receptor type 2(CCR2)-overexpressing HUC-MSCs(ExoCCR2)can enhance the therapeutic effects on PSCI and the possible underlying mechanisms have not been studied.AIM To investigate the effects of ExoCtrl on PSCI and whether ExoCCR2 can enhance therapeutic effects on PSCI.METHODS Transmission electron microscopy,qNano®particles analyzer,and Western blotting were employed to determine the morphology and CCR2 expression of ExoCtrl or ExoCCR2.ELISA was used to study the binding capacity of exosomes to CC chemokine ligand 2(CCL2)in vivo.After the intravenous injection of ExoCtrl or ExoCCR2 into experimental rats,the effect of ExoCtrl and ExoCCR2 on PSCI was assessed by Morris water maze.Remyelination and oligodendrogenesis were analyzed by Western blotting and immunofluorescence microscopy.QRT-PCR and immunofluorescence microscopy were conducted to compare the microglia/macrophage polarization.The infiltration and activation of hematogenous macrophages were analyzed by Western blotting and transwell migration analysis.RESULTS CCR2-overexpressing HUC-MSCs loaded the CCR2 receptor into their exosomes.The morphology and diameter distribution between ExoCtrl and ExoCCR2 showed no significant difference.ExoCCR2 bound significantly to CCL2 but ExoCtrl showed little CCL2 binding.Although both ExoCCR2 and ExoCtrl showed beneficial effects on PSCI,oligodendrogenesis,remyelination,and microglia/macrophage polarization,ExoCCR2 exhibited a significantly superior beneficial effect.We also found that ExoCCR2 could suppress the CCL2-induced macrophage migration and activation in vivo and in vitro,compared with ExoCtrl treated group.CONCLUSION CCR2 over-expression enhanced the therapeutic effects of exosomes on the experimental PSCI by promoting M2 microglia/macrophage polarization,enhancing oligodendrogenesis and remyelination.These therapeutic effects are likely through suppressing the CCL2-induced hematogenous macrophage migration and activation.Key words:Cognitive impairment;Stroke;Exosomes;C-C chemokine receptor type 2;Microglia/macrophage polarization;Remyelination.

PI3K/SHIP2/PTEN pathway in cell polarity and hepatitis C virus pathogenesis

Hepatitis C virus(HCV) infects hepatocytes, polarized cells in the liver. Chronic HCV infection often leads to steatosis, fibrosis, cirrhosis and hepatocellular carcinoma, and it has been identified as the leading cause of liver transplantation worldwide. The HCV replication cycle is dependent on lipid metabolism and particularly an accumulation of lipid droplets in host cells. Phosphoinositides(PIs) are minor phospholipids enriched in different membranes and their levels are tightly regulated by specific PI kinases and phosphatases. PIs are implicated in a vast array of cellular responses that are central to morphogenesis, such as cytoskeletal changes, cytokinesis and the recruitment of downstream effectors to govern mechanisms involved in polarization and lumen formation. Important reviews of the literature identified phosphatidylinositol(Ptd Ins) 4-kinases, and their lipid products Ptd Ins(4)P, as critical regulators of the HCV life cycle. SH2-containing inositol polyphosphate 5-phosphatase(SHIP2), phosphoinositide 3-kinase(PI3K) and their lipid products Ptd Ins(3,4)P2 and Ptd Ins(3,4,5)P3, respectively, play an important role in the cell membrane and are key to the establishment of apicobasal polarity and lumen formation. In this review, we will focus on these new functions of PI3 K and SHIP2, and their deregulation by HCV, causing a disruption of apicobasal polarity, actin organization and extracellular matrix assembly. Finally we will highlight the involvement of this pathway in the event of insulin resistance and nonalcoholic fatty liver disease related to HCV infection.

LATE CENOZOIC LITHOLOGY AND MAGNETIC POLARITY STRATIGRAPHY IN THE JIUXI BASIN: IMPLICATIONS FOR TECTONIC EVENTS OF THE WEST QILIAN MT

The synorogenic sediments in the foreland basins preserve a potential record of varied depositional environments that respond to and are largely controlled by tectonic events of the mountain along the basin margin. In the exploration of the uplift process of the Qinghai—Tibet Plateau, research on the foreland basins around the plateau is of vital significance. Much work have been carried out on the southern margin along the Himalayas and on the eastern margin, however, seldom work was reported along the northern boundary of the plateau, this have blocked the generation of an integrative image of the evolution of the entire plateau. The Gansu Corridor, located on the northeast margin of the Qinghai—Tibet Plateau, is a Cenozoic foreland basin system formed due to the northward overthrust and overload of the plateau. Thousand of meters detrital sediments derived mainly from denudation of the Qilian orogen have deposited in the basin since Oligocene. Here we report a detailed lithologic and magnetostratigraphic work carried out on the late Cenozoic sedimentary sequences in the Jiuxi Basin, western Gansu Corridor, to investigate the neotectonism of the western Qilian Mt.

预防性使用维生素C可通过调节巨噬细胞极化和肺泡上皮细胞凋亡缓解放射性肺损伤

随着新冠肺炎在我国的持续流行和放射疗法普遍开展,放射性肺损伤(RILI)也逐渐成为了一个备受关注的临床问题。放射性肺损伤的发病机制十分复杂,该过程涉及到肺泡巨噬细胞极化状态的失衡以及肺泡上皮细胞凋亡水平的上调。既往的研究表明,维生素C是重要的抗氧化物质,预防性使用维生素C可有效治疗急性肺损伤。然而,预防性使用维生素C是否可有效预防或治疗放射性物质导致的肺损伤,以及其具体分子机制尚待研究。该研究旨在探讨预防性使用维生素C处理肺泡巨噬细胞系RAW 264.7,以及人肺上皮细胞BEAS-2B是否能有效控制巨噬细胞的异常极化及其肺上皮细胞的异常凋亡,并研究该过程中的分子机制。本研究发现,放射性X线照射4周、8周后,巨噬细胞M1极化状态标志物例如iNOS会发生显著的表达上调(P<0.05),预防性使用维生素C处理巨噬细胞以及肺上皮细胞,能缓解放射性外照射导致的巨噬细胞极化状态紊乱以及肺泡上皮细胞凋亡,具体表现为切割胱天蛋白酶(cleaved caspase3)水平的表达下调。此外,预防性应用维生素C处理能抑制放射性外照射激活的MAPK信号通路。进一步的研究结果表明,对MAPK通路的抑制作用是抑制巨噬细胞M1极化以及肺上皮细胞凋亡的关键。总之,我们的研究结果提示,维生素C能通过抑制巨噬细胞M1极化/促进巨噬细胞M2极化以及缓解肺泡上皮细胞凋亡而发挥急性放射性肺损伤的保护作用。该研究将更深入地认识维生素C对放射性肺损伤预防作用的过程与机制。

Thin Film Characterization Using Rotating Polarizer Analyzer Ellipsometer with a Speed Ratio 1:3

In a recent previous work, we proposed a rotating polarizer-analyzer ellipsometer (RPAE) in which the two elements are rotating synchronously in the same direction with a speed ratio 1:3. We applied this technique to bulk samples. In this work, we present theoretically the characterization of 100 nm SiO2 thin film using this spectroscopic RPAE. We assume a structure consisting of air (ambient)/SiO2 (thin film)/c-Si (substrate). The ellipsometric parameters ψ and Δ are calculated when a clean signal is received by the detector and when a hypothetical noise is imposed on this signal. The film thickness and the optical constants of the film are calculated for the noisy signal in the spectrum range 200 - 800 nm. The results are compared with the proposed thickness and with the accepted values for SiO2 optical constants.

Polarization of ∧'s Produced in S+Pb Collisions at 200 GeV/c per Nucleon

The ∧^(0) polarization is analyzed with the experinental data in S+Pb collisions at energy 200GeV per nucleon,which were produced at SPS of CERN.The snall.polarization rxalue and thecorrelation betrween the transverse polarization and transverse momentum were obtained in Feyn-man parameter x_(F)>0,r_(F)<0 and all xp.The results show that the non-polarization of lamb-da appears in the vegion of larger transterse momenta,say,at 1-2 GeV/c in P_(T).The originsof polarization reduction are discussed.

Correlation Between Transverse Polarization and Rapidity of Λ Produced in S+Pb Collisions at 200GeV/c Per Nucleon

The dependence of transverse polarization on rapidity ofΛproduced in S+Pb collisions at 200 GeV/c per nucleon has been studied.The original experimental data are from NA36 Collaboration at SPS of CERN.The precession effect ofΛspin,owing to the interactions between spin moment ofΛand the magnetic field on the path whichΛpasses through,on the polarization has been taken into account.The results show that there are slight differences for the transverse polarization in different rapidity-region.The origins for these results have been analysed and discussed.

Exosomes derived from inflammatory myoblasts promote M1 polarization and break the balance of myoblast proliferation/differentiation

BACKGROUND Acute muscle injuries are one of the most common injuries in sports.Severely injured muscles are prone to re-injury due to fibrotic scar formation caused by prolonged inflammation.How to regulate inflammation and suppress fibrosis is the focus of promoting muscle healing.Recent studies have found that myoblasts and macrophages play important roles in the inflammatory phase following muscle injury;however,the crosstalk between these two types of cells in the inflammatory environment,particularly the exosome-related mechanisms,had not been well studied.AIM To evaluate the effects of exosomes from inflammatory C2C12 myoblasts(IFC2C12-Exos)on macrophage polarization and myoblast proliferation/differentiation.METHODS A model of inflammation was established in vitro by lipopolysaccharide stimulation of myoblasts.C2C12-Exos were isolated and purified from the supernatant of myoblasts by gradient centrifugation.Multiple methods were used to identify the exosomes.Gradient concentrations of IF-C2C12-Exos were added to normal macrophages and myoblasts.PKH67 fluorescence tracing was used to identify the interaction between exosomes and cells.Microscopic morphology,Giemsa stain,and immunofluorescence were carried out for histological analysis.Additionally,ELISA assays,flow cytometry,and western blot were conducted to analyze molecular changes.Moreover,myogenic proliferation was assessed by the BrdU test,scratch assay,and CCK-8 assay.RESULTS We found that the PKH-67-marked C2C12-Exos can be endocytosed by both macrophages and myoblasts.IF-C2C12-Exos induced M1 macrophage polarization and suppressed the M2 phenotype in vitro.In addition,these exosomes also stimulated the inflammatory reactions of macrophages.Further-more,we demonstrated that IF-C2C12-Exos disrupted the balance of myoblast proliferation/differentiation,leading to enhanced proliferation and suppressed fibrogenic/myogenic differentiation.CONCLUSION IF-C2C12-Exos can induce M1 polarization,resulting in a sustained and aggravated inflammatory environment that impairs myoblast differentiation,and leads to enhanced myogenic proliferation.These results demonstrate why prolonged inflammation occurs after acute muscle injury and provide a new target for the regulation of muscle regeneration.

Interplay among cellular polarization,lipoprotein metabolism and hepatitis C virus entry

Hepatitis C virus (HCV) infects more than three million new individuals worldwide each year. In a high percent age of patients, acute infections become chronic, eventually progressing to fibrosis, cirrhosis, and hepatocellular carcinoma. Given the lack of effective prophylactic or therapeutic vaccines, and the limited sustained virological response rates to current therapies, new approaches are needed to prevent, control, and clear HCV infection.Entry into the host cell, being the first step of the viral cycle, is a potential target for the design of new antiviral compounds. Despite the recent discovery of the tight junction-associated proteins claudin-1 and occludin as HCV co-receptors, which is an important step towards the understanding of HCV entry, the precise mechanisms are still largely unknown. In addition, increasing evidence indicates that tools that are broadly employed to study HCV infection do not accurately reflect the real process in terms of viral particle composition and host cell phenotype. Thus, systems that more closely mimic natural infection are urgently required to elucidate the mechanisms of HCV entry, which will in turn help to design antiviral strategies against this part of the infection process.

Dielectric and Spontaneous Polarization Studies of a Ferroelectric Chiral Smectic Liquid Crystal

Dielectric relaxation and spontaneous polarization studies have been carried out in a low molar mass organosiloxane liquid crystal, TSi10PPBN4 which displays a wide temperature range of de Vries Smectic A* and chiral Smectic C* phases. The temperature variation of the permittivity and dielectric loss studied are found to have anomalous variations in the vicinity of the phase transitions. The frequency variation of permittivity and dielectric loss are also studied in the de Vries Smectic A* and Smectic C* phases. The relaxation frequency, fR is found to decrease with decreasing temperature. Temperature variation of fR is found to follow an Arrhenius shift from which the activation energy is estimated. The thermal variation of the spontaneous polarization, Ps is measured using the polarization reversal technique in the Smectic C* phase. Ps increases initially, reaches a peak value and then drops drastically in the vicinity of the SmC*-SmA*de Vries phase transition.

High stability and low noise laser-diode end-pumped Nd:YAG ceramic passively Q-switched laser at 1123 nm based on a Ti_(3)C_(2)T_(x)-PVA saturable absorber

We report a high repetition frequency, high power stability and low laser noise laser-diode(LD) end-pumped Nd: YAG ceramic passively Q-switched laser at 1123 nm based on a Ti_(3)C_(2)T_(x)-polyvinyl alcohol(PVA) film as a saturable absorber(SA). A Brewster polarizer(BP) and a birefringent crystal(BC) are incorporated to enable frequency selection and filtering for the passively Q-switched 1123 nm pulsed laser to improve the power stability and reduce the noise. When the pump power is 5.1 W, an average output power of 457.9 m W is obtained, corresponding to a repetition frequency of 1.09 MHz,a pulse width of 56 ns, a spectral line width of 0.65 nm, a power instability of ±0.92%, and a laser noise of 0.89%.The successful implementation of the “Ti_(3)C_(2)T_(x)-PVA film passively Q-switching” combined with “frequency selection and filtering of BP + BC” technology path provides a valuable reference for developing pulsed laser with high repetition frequency, high stability and low noise.

两次森林火灾的双偏振雷达探测特征分析

通过对2020年3月17日山东烟台、4月23日山东青岛发生的两次森林火灾的双偏振雷达探测资料进行分析,结果表明:(1)森林火灾燃烧产生的灰烬上升到空中,随环境风向下风方飘散,形成烟羽,烟羽的雷达反射率因子在20 dBZ左右,火灾点上空很小范围最大值达36 dBZ。(2)两次山火的灰烬飘浮物随环境风向下游扩散的最远距离分别是65 km和94 km,最高上升高度分别达3.7 km和4.8 km。(3)追踪烟羽的前沿可以估算相应高度上环境风的速度;两次火灾都发生在晴天,环境风速不大,除了烟羽边缘位置速度谱宽值较大外,其余部分速度谱宽都很小,大部分为1~2 m·s^(-1)。(4)相关系数(C_(c))、差分反射率(Z_(DR))和差分相移(ΦDP)探测到的范围与雷达反射率因子的演变形态和面积接近,同样随烟羽的扩散而逐渐增大;C_(c)值非常小,大多数区域小于0.9,少数距离库库元的C_(c)值低于0.5;火灾点上空的Z_(DR)值有相对较小的区域,为2~3 dB,而火灾点下风方,大部分Z_(DR)值都很大,大多超过5 dB。(5)两次森林火灾的双偏振探测特征表明,产生回波的反射物主要是干草、松针等燃烧后的灰烬形成的水平尺度较大的非球形粒子反射物。

一种对称极坐标图像模糊C均值聚类的电主轴失衡故障诊断方法

为解决电主轴转子不平衡故障的可视化智能识别问题,提出了一种对称极坐标图像和模糊C均值(FCM)聚类相结合的失衡故障诊断新方法。首先对转子时域振动信号进行经验模态分解降噪,按对称极坐标方法将其转化为二维雪花图像,通过灰度共生矩阵,提取雪花图像二维特征参数;然后对已知样本信号的特征参数组建故障特征向量,标准化后作为FCM输入,得到分类矩阵和聚类中心;最后计算待测样本和已知故障样本聚类中心贴进度,实现失衡故障识别和分类。在某电主轴系统平台上完成了1800 r/min时转子3种不同失衡状态的诊断试验,在对45组小样本识别中该方法的分类准确率达到73%。

C波段偏振雷达数据预处理及在降水估计中的应用

利用移动式C波段双线偏振雷达(PCDJ)观测资料,对安徽定远2013年6—7月3次降水过程进行数据处理方法和降水估计效果的分析,提出了优化的C波段双偏振雷达数据处理方案,在优化的R(Z_H)关系条件下,分析了降水估测的效果。对比分析中值滤波和小波分析方法对差传播相移Φ_(DP)数据处理的效果,发现小波分析方法对Φ_(DP)数据处理具有明显的改善作用;采用变距离法对小波分析处理后的Φ_(DP)数据进行拟合得到的K_(DP),数据的连续性和平滑度较好,且应用于降水估计的精度更高;利用较好的数据处理方法(小波分析)拟合得到的K_(DP)对水平反射率因子Z_H进行雨区衰减校正;以及利用SA雷达与PCDJ雷达衰减校正后的数据进行对比观测,表明PCDJ雷达回波强度比SA雷达偏弱。对两部雷达进行定量降水估计,并与地面雨量计小时降水量资料进行对比分析,结果表明:当降水强度>5 mm·h^(-1)时,PCDJ雷达利用R(Z_H,K_(DP))关系估测降水的精度比R(Z_H)关系高,当降水强度<5 mm·h^(-1)时,则相反;SA雷达与PCDJ雷达均基于反射率因子估测降水,SA雷达的效果更好;但在降水强度>5 mm·h^(-1)时,PCDJ雷达基于R(Z_H,K_(DP))关系估测降水较SA雷达基于R(Z)关系方法具有更明显的优势。