We report on the use of a fiber-based Mueller-matrix optical coherence tomography(OCT)system with continuous source-polarization modulation for in vivo imaging of early stages of skin cancer in SENCAR mice.A homemade ...We report on the use of a fiber-based Mueller-matrix optical coherence tomography(OCT)system with continuous source-polarization modulation for in vivo imaging of early stages of skin cancer in SENCAR mice.A homemade hand-held probe with integrated optical scanning and beam delivering optics was coupled in the sample arm.The OCT images show the morphological changes in skin resulting from pre-cancerous papilloma formations that are consistent with histology,thus demonstrating the system’s potential for early skin cancer detection.展开更多
Polarization-sensitive optical coherence tomo- graphy （PS-OCT） enables depth-resolved mapping of sample polarization information, such as phase-retardation and optical axis orientation, which is particularly useful ...Polarization-sensitive optical coherence tomo- graphy （PS-OCT） enables depth-resolved mapping of sample polarization information, such as phase-retardation and optical axis orientation, which is particularly useful when the nano-scale organization of tissue that are difficult to be observed in the intensity images of a regular optical coherence tomography （OCT）. In this review, we survey two types of methods and systems of PS-OCT. The first type is PS-OCT with single input polarization state, which contain bulk optics or polarization maintaining fiber （PMF） based systems and single-mode fiber （SMF） based systems. The second type is PS-OCT with two different input polarization states, which contain SMF based systems and PMF based systems, through either time, frequency, or depth multiplexing. In addition, representa- tive biomedical applications using PS-OCT, such as retinal imaging, skin cancer detection, and brain mapping, are demonstrated.展开更多
Spectral domain polarization-sensitive optical coherence tomography （SDPS-OCT） is a depth-resolved polarization-sensitive interferometry which integrates polarization optics into spectral domain optical co- herence ...Spectral domain polarization-sensitive optical coherence tomography （SDPS-OCT） is a depth-resolved polarization-sensitive interferometry which integrates polarization optics into spectral domain optical co- herence tomography （SD-OCT）. This configuration can obtain birefringence information of samples and improve the imaging speed. In this paper, horizontally polarized light is used to replace natural light of the source. Then, right-rotated circularly polarized light is the incident sample light. To obtain two orthogonal components of the polarized interferogram, the reflected light of the reference arm is set to be 45° linearly polarized light. These two components are acquired by two spectrometers synchronously. The system was employed to achieve 12.8μm axial resolution and 4.36μm transverse resolution. We have imaged in vitro chicken tendon and muscle tissues with these svstem.展开更多
Background:The retina has been used to study the pathophysiology of multiple sclerosis(MS).Peripapillary retinal nerve fiber layer(pRNFL)thinning has been suggested as an ocular biomarker of neurodegeneration in MS.Th...Background:The retina has been used to study the pathophysiology of multiple sclerosis(MS).Peripapillary retinal nerve fiber layer(pRNFL)thinning has been suggested as an ocular biomarker of neurodegeneration in MS.The goal of this project was to determine the birefringence of the pRNFL by measuring the fiber birefringence using polarization sensitive optical coherence tomography(PS-OCT).Methods:Sixty-six MS patients without history of optic neuritis(age:39.9±11.0 yrs.old,53 females and 13 males)and 66 age-and gender-matched normal controls(age:40.7±11.4 yrs.old)were recruited.Custom built PS-OCT was used to measure phase retardation per unit depth(PR/UD,proportional to the birefringence)and pRNFL thickness in each quadrant of the pRNFL.In addition,clinical manifestation was used to correlate with the pRNFL birefringence.Results:The pRNFL was thinner in the temporal and inferior quadrants in MS patients compared with normal controls(P<0.05).The PR/UD of the pRNFL was significantly decreased in MS patients(P<0.05)in all quadrants except for the nasal quadrant.In both groups,the PR/UD from all four quadrants was not related to the averaged pRNFL thickness(P>0.05).In MS patients,the PR/UD was not related to the expanded disability status scale(EDSS)nor disease duration(r ranged from−0.17 to 0.02,P>0.05).Conclusion:This is the first study using PS-OCT to study the pRNFL birefringence in MS patients.Decreased birefringence of the pRNFL may indicate microtubule abnormality,and could be a potential biomarker for detecting early neurodegeneration in MS.展开更多
基金the Department of the Army(Cooperative Agreement Number:DAMD17-97-2-7016)the National Institutes of Health(R01 CA092415)。
文摘We report on the use of a fiber-based Mueller-matrix optical coherence tomography(OCT)system with continuous source-polarization modulation for in vivo imaging of early stages of skin cancer in SENCAR mice.A homemade hand-held probe with integrated optical scanning and beam delivering optics was coupled in the sample arm.The OCT images show the morphological changes in skin resulting from pre-cancerous papilloma formations that are consistent with histology,thus demonstrating the system’s potential for early skin cancer detection.
文摘Polarization-sensitive optical coherence tomo- graphy （PS-OCT） enables depth-resolved mapping of sample polarization information, such as phase-retardation and optical axis orientation, which is particularly useful when the nano-scale organization of tissue that are difficult to be observed in the intensity images of a regular optical coherence tomography （OCT）. In this review, we survey two types of methods and systems of PS-OCT. The first type is PS-OCT with single input polarization state, which contain bulk optics or polarization maintaining fiber （PMF） based systems and single-mode fiber （SMF） based systems. The second type is PS-OCT with two different input polarization states, which contain SMF based systems and PMF based systems, through either time, frequency, or depth multiplexing. In addition, representa- tive biomedical applications using PS-OCT, such as retinal imaging, skin cancer detection, and brain mapping, are demonstrated.
文摘Spectral domain polarization-sensitive optical coherence tomography （SDPS-OCT） is a depth-resolved polarization-sensitive interferometry which integrates polarization optics into spectral domain optical co- herence tomography （SD-OCT）. This configuration can obtain birefringence information of samples and improve the imaging speed. In this paper, horizontally polarized light is used to replace natural light of the source. Then, right-rotated circularly polarized light is the incident sample light. To obtain two orthogonal components of the polarized interferogram, the reflected light of the reference arm is set to be 45° linearly polarized light. These two components are acquired by two spectrometers synchronously. The system was employed to achieve 12.8μm axial resolution and 4.36μm transverse resolution. We have imaged in vitro chicken tendon and muscle tissues with these svstem.
基金Supported by the National Multiple Sclerosis Society,NIH Center Grant P30 EY014801a grant from Research to Prevent Blindness(RPB).
文摘Background:The retina has been used to study the pathophysiology of multiple sclerosis(MS).Peripapillary retinal nerve fiber layer(pRNFL)thinning has been suggested as an ocular biomarker of neurodegeneration in MS.The goal of this project was to determine the birefringence of the pRNFL by measuring the fiber birefringence using polarization sensitive optical coherence tomography(PS-OCT).Methods:Sixty-six MS patients without history of optic neuritis(age:39.9±11.0 yrs.old,53 females and 13 males)and 66 age-and gender-matched normal controls(age:40.7±11.4 yrs.old)were recruited.Custom built PS-OCT was used to measure phase retardation per unit depth(PR/UD,proportional to the birefringence)and pRNFL thickness in each quadrant of the pRNFL.In addition,clinical manifestation was used to correlate with the pRNFL birefringence.Results:The pRNFL was thinner in the temporal and inferior quadrants in MS patients compared with normal controls(P<0.05).The PR/UD of the pRNFL was significantly decreased in MS patients(P<0.05)in all quadrants except for the nasal quadrant.In both groups,the PR/UD from all four quadrants was not related to the averaged pRNFL thickness(P>0.05).In MS patients,the PR/UD was not related to the expanded disability status scale(EDSS)nor disease duration(r ranged from−0.17 to 0.02,P>0.05).Conclusion:This is the first study using PS-OCT to study the pRNFL birefringence in MS patients.Decreased birefringence of the pRNFL may indicate microtubule abnormality,and could be a potential biomarker for detecting early neurodegeneration in MS.
