The lower seed setting is one of the major hindrances which face grain yield in rice. One of the main reasons to cause low spikelet fertility （seed setting） is male sterility or pollen abortion. Notably, pollen abor...The lower seed setting is one of the major hindrances which face grain yield in rice. One of the main reasons to cause low spikelet fertility （seed setting） is male sterility or pollen abortion. Notably, pollen abortion has been frequently observed in advanced progenies of rice. In the present study, 149 BC2F6 individuals with significant segregation in spikelet fertility were generated from the cross between N040212 （indica） and Nipponbare （japonica） and used for primary gene mapping. Three QTLs, qSS-1, qSS-7 and qSS-9 at chromosomes 1, 7 and 9, respectively, were found to be associated with seed setting. The recombinant analysis and the physical mapping information from publicly available resources exhibited that the qSS-1, qSS-7 and qSS-9 loci were mapped to an interval of 188, 701 and 3741 kb, respectively. The seed setting responsible for QTL qSS-1 was further fine mapped to 93.5 kb by using BC2F7 population of 1 849 individuals. There are 16 possible putative genes in this 93.5 kb region. Pollen vitality tests and artificial pollination indicated that the male gamete has abnormal pollen while the female gamete was normal. These data showed that low seed setting rate relative to qSS-1 may be caused by abnormal pollen grains. These results will be useful for cloning, functional analysis of the target gene governing spikelet fertility （seed setting） and understanding the genetic bases of pollen sterility.展开更多
Biomization provides an important way to assign pollen taxa to biomes and to simulate palaeo-vegetation patterns, so that pollen data can be mapped to reconstruct biogeography and climate. The authors have tested the ...Biomization provides an important way to assign pollen taxa to biomes and to simulate palaeo-vegetation patterns, so that pollen data can be mapped to reconstruct biogeography and climate. The authors have tested the applicability of this procedure to assign modern pollen surface samples from China to biomes. The procedure successfully delineated the major vegetation types of China. When the same procedure was applied to 6 ka B.P. fossil pollen samples, the reconstructions showed that the forest zones were systematically shifted northwards ca. 300\500 km beyond their present northern limits in eastern China; the area of desert and steppe vegetation was reduced compared to the present in northwestern China; the area of tundra was reduced largely on the Tibetan Plateau. This research is a contribution to the project of BIOME 6000 in Pacific-Asian regions.展开更多
To understand the molecular mechanism of male reproductive development in the model crop rice,we isolated a complete male sterile mutant post-meiotic deficient anther1 （pda1） from a γ-ray-treated rice mutant librar...To understand the molecular mechanism of male reproductive development in the model crop rice,we isolated a complete male sterile mutant post-meiotic deficient anther1 （pda1） from a γ-ray-treated rice mutant library.Genetic analysis revealed that the pda1 mutant was controlled by a recessive nucleus gene.The pda1 mutant anther seemed smaller with white appearance.Histological analysis demonstrated that the pda1 mutant anther undergoes normal early tapetum development without obvious altered meiosis.However,the pda1 mutant displayed obvious defects in postmeiotic tapetal development,abnormal degeneration occurred in the tapetal cells at stage 9 of anther development.Also we observed abnormal lipidic Ubisch bodies from the tapetal layer of the pda1 mutant,causing no obvious pollen exine formation.RT-PCR analysis indicated that the expression of genes involved in anther development including GAMYB,OsC4 and Wax-deficient anther1 （WDA1） was greatly reduced in the pda1 mutant anther.Using map-based cloning approach,the PDA1 gene was finely mapped between two markers HLF610 and HLF627 on chromosome 6 using 3,883 individuals of F2 population.The physical distance between HLF610 and HLF627 was about 194 kb.This work suggests that PDA1 is required for post-meiotic tapetal development and pollen/microspore formation in rice.展开更多
基金supported by the National Key Technologies R&D Program of China during the 12th Five-Year Plan period (2013BAD01B02-15 and 2015BAD02B01)the 948 Project of Minstry of Agriculture, China (2011-G2B and 2011-G1 (2)-25)
文摘The lower seed setting is one of the major hindrances which face grain yield in rice. One of the main reasons to cause low spikelet fertility （seed setting） is male sterility or pollen abortion. Notably, pollen abortion has been frequently observed in advanced progenies of rice. In the present study, 149 BC2F6 individuals with significant segregation in spikelet fertility were generated from the cross between N040212 （indica） and Nipponbare （japonica） and used for primary gene mapping. Three QTLs, qSS-1, qSS-7 and qSS-9 at chromosomes 1, 7 and 9, respectively, were found to be associated with seed setting. The recombinant analysis and the physical mapping information from publicly available resources exhibited that the qSS-1, qSS-7 and qSS-9 loci were mapped to an interval of 188, 701 and 3741 kb, respectively. The seed setting responsible for QTL qSS-1 was further fine mapped to 93.5 kb by using BC2F7 population of 1 849 individuals. There are 16 possible putative genes in this 93.5 kb region. Pollen vitality tests and artificial pollination indicated that the male gamete has abnormal pollen while the female gamete was normal. These data showed that low seed setting rate relative to qSS-1 may be caused by abnormal pollen grains. These results will be useful for cloning, functional analysis of the target gene governing spikelet fertility （seed setting） and understanding the genetic bases of pollen sterility.
基金This Work was supported by me Major Basic Research Program of Chinese Ministry of science and Technology(No.2003CCA01100)and the National Natural Science Foundation of China(No.30470170).
文摘Biomization provides an important way to assign pollen taxa to biomes and to simulate palaeo-vegetation patterns, so that pollen data can be mapped to reconstruct biogeography and climate. The authors have tested the applicability of this procedure to assign modern pollen surface samples from China to biomes. The procedure successfully delineated the major vegetation types of China. When the same procedure was applied to 6 ka B.P. fossil pollen samples, the reconstructions showed that the forest zones were systematically shifted northwards ca. 300\500 km beyond their present northern limits in eastern China; the area of desert and steppe vegetation was reduced compared to the present in northwestern China; the area of tundra was reduced largely on the Tibetan Plateau. This research is a contribution to the project of BIOME 6000 in Pacific-Asian regions.
基金supported by the Funds from the National Key Basic Research Developments Program of the Ministry of Science and Technology, China (No. 2009CB941500)Shanghai Leading Academic Discipline Project (No. B205)
文摘To understand the molecular mechanism of male reproductive development in the model crop rice,we isolated a complete male sterile mutant post-meiotic deficient anther1 （pda1） from a γ-ray-treated rice mutant library.Genetic analysis revealed that the pda1 mutant was controlled by a recessive nucleus gene.The pda1 mutant anther seemed smaller with white appearance.Histological analysis demonstrated that the pda1 mutant anther undergoes normal early tapetum development without obvious altered meiosis.However,the pda1 mutant displayed obvious defects in postmeiotic tapetal development,abnormal degeneration occurred in the tapetal cells at stage 9 of anther development.Also we observed abnormal lipidic Ubisch bodies from the tapetal layer of the pda1 mutant,causing no obvious pollen exine formation.RT-PCR analysis indicated that the expression of genes involved in anther development including GAMYB,OsC4 and Wax-deficient anther1 （WDA1） was greatly reduced in the pda1 mutant anther.Using map-based cloning approach,the PDA1 gene was finely mapped between two markers HLF610 and HLF627 on chromosome 6 using 3,883 individuals of F2 population.The physical distance between HLF610 and HLF627 was about 194 kb.This work suggests that PDA1 is required for post-meiotic tapetal development and pollen/microspore formation in rice.
