Three polysaccharides, TAA, TAB, TAC have been isolated from the pollen of Typha angustifolia L. , and purified by hot water extraction, alcohol precipitation, Sevage method, gel filtration (Sephadex G50) and ion-exch...Three polysaccharides, TAA, TAB, TAC have been isolated from the pollen of Typha angustifolia L. , and purified by hot water extraction, alcohol precipitation, Sevage method, gel filtration (Sephadex G50) and ion-exchange chromatography on DEAE Sephadex A25 and DEAE-cellulose. Their purities were tested by gel filtration and electrophoresis. Their structures were characterized by paper chromatography of the hydrolysis products, GLC analysis of the alditol acetates, alkylation analysis, 1H NMR and 13C NMR spectra. TAA is mainly composed of a-L-arabinofuanose, β-D-galactose and α-D-galacturonic acid, while the backbones of TAB and TAC are composed of (1→5)-linked α- L-arabinofuranosyl residues.展开更多
Three polysaccharides TAA,TAB and TAC were purified from the pollen of Typha An- gustifilia L..TAA is mainly composed of α-L-arabinofuranose,β—D—galactose and α—D—galac- turonic acid.TAB and TAC contain the bac...Three polysaccharides TAA,TAB and TAC were purified from the pollen of Typha An- gustifilia L..TAA is mainly composed of α-L-arabinofuranose,β—D—galactose and α—D—galac- turonic acid.TAB and TAC contain the backbone mainly composed of (1→5)—linked—L—arabi- nosyl residue.展开更多
Objective: To observe the lipid-lowering effect of Typha angustifolia extract(TAE) in Sprague Dawley rats.Methods: The rats were randomly divided into six groups, including the hypercholesterolemic diet(HYD)group. Ad ...Objective: To observe the lipid-lowering effect of Typha angustifolia extract(TAE) in Sprague Dawley rats.Methods: The rats were randomly divided into six groups, including the hypercholesterolemic diet(HYD)group. Ad libitum food was given to the animals for 60 d to establish dyslipidemia models. The rats were received an ig administration of 1 mL medicine daily. After 120 d the animals were sacrificed by cervical dislocation; Blood was collected to measure total cholesterol(TC), triglycerides(TG), low-density lipoprotein cholesterol(LDL-C), high-density lipoprotein-cholesterol(HDL-C), nitric oxide(NO), and endothelin(ET) plasma concentration; Livers were collected to measure ApoE mRNA and protein expression; Morphologic changes of aorta ventralis tissue were also observed.Results: Compared with HYD group, TAE had the ability of reducing TC, TG, LDL-C, NO, and ET(P < 0.01),thereby increasing ApoE mRNA and protein expression of the liver(P < 0.01).Conclusion: These results suggested that TAE was capable of effectively decreasing the circulating lipids levels and enhancing the protective effects of artery.展开更多
文摘Three polysaccharides, TAA, TAB, TAC have been isolated from the pollen of Typha angustifolia L. , and purified by hot water extraction, alcohol precipitation, Sevage method, gel filtration (Sephadex G50) and ion-exchange chromatography on DEAE Sephadex A25 and DEAE-cellulose. Their purities were tested by gel filtration and electrophoresis. Their structures were characterized by paper chromatography of the hydrolysis products, GLC analysis of the alditol acetates, alkylation analysis, 1H NMR and 13C NMR spectra. TAA is mainly composed of a-L-arabinofuanose, β-D-galactose and α-D-galacturonic acid, while the backbones of TAB and TAC are composed of (1→5)-linked α- L-arabinofuranosyl residues.
文摘Three polysaccharides TAA,TAB and TAC were purified from the pollen of Typha An- gustifilia L..TAA is mainly composed of α-L-arabinofuranose,β—D—galactose and α—D—galac- turonic acid.TAB and TAC contain the backbone mainly composed of (1→5)—linked—L—arabi- nosyl residue.
基金supported by Research Fund and Natural Science Foundation of Yichang City in Hubei Province,China(Nos.KFJ2013009A14301-06)
文摘Objective: To observe the lipid-lowering effect of Typha angustifolia extract(TAE) in Sprague Dawley rats.Methods: The rats were randomly divided into six groups, including the hypercholesterolemic diet(HYD)group. Ad libitum food was given to the animals for 60 d to establish dyslipidemia models. The rats were received an ig administration of 1 mL medicine daily. After 120 d the animals were sacrificed by cervical dislocation; Blood was collected to measure total cholesterol(TC), triglycerides(TG), low-density lipoprotein cholesterol(LDL-C), high-density lipoprotein-cholesterol(HDL-C), nitric oxide(NO), and endothelin(ET) plasma concentration; Livers were collected to measure ApoE mRNA and protein expression; Morphologic changes of aorta ventralis tissue were also observed.Results: Compared with HYD group, TAE had the ability of reducing TC, TG, LDL-C, NO, and ET(P < 0.01),thereby increasing ApoE mRNA and protein expression of the liver(P < 0.01).Conclusion: These results suggested that TAE was capable of effectively decreasing the circulating lipids levels and enhancing the protective effects of artery.
