传粉昆虫是生物多样性重要组成部分,昆虫传粉生境建设能有效减缓植物授粉危机,有利于城市生物多样性保护和促进自然教育。文章通过检索文献库CNKI和Web of Science近20年相关文献,采用文献计量法、内容分析法,对昆虫传粉生境营建相关的...传粉昆虫是生物多样性重要组成部分,昆虫传粉生境建设能有效减缓植物授粉危机,有利于城市生物多样性保护和促进自然教育。文章通过检索文献库CNKI和Web of Science近20年相关文献,采用文献计量法、内容分析法,对昆虫传粉生境营建相关的规划与设计、建设与管理研究成果进行梳理。结果表明:规划方面,注重该类绿地与区域生态网络和城市专项规划融合;设计方面,强调植物选用遵循“双高”“本土”“多样化”原则,并采用“高密群植”“防风向阳”方式配置;建设方面,提倡保留多种地表,增加与雨水花园、绿色屋顶等生态景观布置和栖息设施举措;管理方面,建议纳入4种特色日常管理措施,并提出管理部门的政策支持能助力实现昆虫多样性目标。文章最后提出传粉生境营建研究还存在意义彰显不足、实践应用待加强,以及我国发展缓慢等相关思考,旨在对我国城市昆虫传粉生境营建与城市生物多样性保护提供研究参考。展开更多
Prezygotic isolation is important for successful fertilization in rice, significantly affecting yield. This study focused on F_(5:6) generation plants derived from inter-subspecific crosses(Nipponbare × KDML105) ...Prezygotic isolation is important for successful fertilization in rice, significantly affecting yield. This study focused on F_(5:6) generation plants derived from inter-subspecific crosses(Nipponbare × KDML105) with low(LS) and high seed-setting rates(HS), in which normal pollen fertility was observed. However, LS plants showed a reduced number of pollen grains adhering to the stigma and fewer pollen tubes reaching the ovules at 4-5 h post-pollination, compared with HS plants. Bulked segregant RNA-Seq analysis of pollinated pistils from the HS and LS groups revealed 249 and 473 differentially expressed genes(DEGs), respectively. Kyoto Encyclopedia of Genes and Genomes analysis of the HS and LS-specific DEGs indicated enrichment in metabolic pathways, pentose and glucuronate interconversions, and flavonoid biosynthesis. Several of these DEGs exhibited co-expression with pollen development genes and formed extensive clusters of co-expression networks. Compared with LS pistils, enzyme genes controlling pectin degradation, such as OsPME35 and OsPLL9, showed similar expression patterns, with higher levels in HS pistils pre-pollination. Os02g0467600, similar to cinnamate 4-hydroxylase gene(CYP73), involved in flavonoid biosynthesis, displayed higher expression in HS pistils post-pollination. Our findings suggest that OsPME35, OsPLL9, and Os02g0467600 contribute to prezygotic isolation by potentially modifying the stigma cell wall(OsPME35 and OsPLL9) and controlling later processes such as pollen-stigma adhesion(Os02g0467600) genes. Furthermore, several DEGs specific to HS and LS were co-localized with QTLs and functional genes associated with spikelet fertility. These findings provide valuable insights for further research on rice spikelet fertility, ultimately contributing to the development of high-yielding rice varieties.展开更多
文摘传粉昆虫是生物多样性重要组成部分,昆虫传粉生境建设能有效减缓植物授粉危机,有利于城市生物多样性保护和促进自然教育。文章通过检索文献库CNKI和Web of Science近20年相关文献,采用文献计量法、内容分析法,对昆虫传粉生境营建相关的规划与设计、建设与管理研究成果进行梳理。结果表明:规划方面,注重该类绿地与区域生态网络和城市专项规划融合;设计方面,强调植物选用遵循“双高”“本土”“多样化”原则,并采用“高密群植”“防风向阳”方式配置;建设方面,提倡保留多种地表,增加与雨水花园、绿色屋顶等生态景观布置和栖息设施举措;管理方面,建议纳入4种特色日常管理措施,并提出管理部门的政策支持能助力实现昆虫多样性目标。文章最后提出传粉生境营建研究还存在意义彰显不足、实践应用待加强,以及我国发展缓慢等相关思考,旨在对我国城市昆虫传粉生境营建与城市生物多样性保护提供研究参考。
基金supported by the Agricultural Research Development Agency of Thailand (Grant No. PRP6405030280)Research Promotion fund for International and Educational Excellence, Thailand (Grant No. 08/2562)。
文摘Prezygotic isolation is important for successful fertilization in rice, significantly affecting yield. This study focused on F_(5:6) generation plants derived from inter-subspecific crosses(Nipponbare × KDML105) with low(LS) and high seed-setting rates(HS), in which normal pollen fertility was observed. However, LS plants showed a reduced number of pollen grains adhering to the stigma and fewer pollen tubes reaching the ovules at 4-5 h post-pollination, compared with HS plants. Bulked segregant RNA-Seq analysis of pollinated pistils from the HS and LS groups revealed 249 and 473 differentially expressed genes(DEGs), respectively. Kyoto Encyclopedia of Genes and Genomes analysis of the HS and LS-specific DEGs indicated enrichment in metabolic pathways, pentose and glucuronate interconversions, and flavonoid biosynthesis. Several of these DEGs exhibited co-expression with pollen development genes and formed extensive clusters of co-expression networks. Compared with LS pistils, enzyme genes controlling pectin degradation, such as OsPME35 and OsPLL9, showed similar expression patterns, with higher levels in HS pistils pre-pollination. Os02g0467600, similar to cinnamate 4-hydroxylase gene(CYP73), involved in flavonoid biosynthesis, displayed higher expression in HS pistils post-pollination. Our findings suggest that OsPME35, OsPLL9, and Os02g0467600 contribute to prezygotic isolation by potentially modifying the stigma cell wall(OsPME35 and OsPLL9) and controlling later processes such as pollen-stigma adhesion(Os02g0467600) genes. Furthermore, several DEGs specific to HS and LS were co-localized with QTLs and functional genes associated with spikelet fertility. These findings provide valuable insights for further research on rice spikelet fertility, ultimately contributing to the development of high-yielding rice varieties.
