The blend film of silk fibroin (SF) and poly(ethylene glycol)400 (PEG400) with a blend ratio of 2/1 (wt/wt) wasprepared simply by dropping a little PEG400 into the SF solution and then casting the mixed aqueous soluti...The blend film of silk fibroin (SF) and poly(ethylene glycol)400 (PEG400) with a blend ratio of 2/1 (wt/wt) wasprepared simply by dropping a little PEG400 into the SF solution and then casting the mixed aqueous solution at 50℃. Theresulting film exhibited much better mechanical properties in the dry and wet state than SF itself, owing to theconformational change of SF in the blends from the random coil to the β-sheet structure and intermolecular hydrogen bondformation between SF and PEG400. Thermogravimetric analysis showed that the initial thermal decomposition temperatureof the blend film was 170℃, which was 80℃ lower than that of SF (250℃) and 20℃ higher than that of PEG400 (150℃),and indicated a Strong interaction between two components of the blend. No crystalline peaks were observed in the X-raydiffraction curve of the blend film. Cell culture test showed that SF/PEG400 was a suitable substrate for the growth of humanumbilical vein endothelial cells (HUVEC).展开更多
基金This work was supported by the National Basic Science Research and Development Grants (973) of China (No. G1999064705).
文摘The blend film of silk fibroin (SF) and poly(ethylene glycol)400 (PEG400) with a blend ratio of 2/1 (wt/wt) wasprepared simply by dropping a little PEG400 into the SF solution and then casting the mixed aqueous solution at 50℃. Theresulting film exhibited much better mechanical properties in the dry and wet state than SF itself, owing to theconformational change of SF in the blends from the random coil to the β-sheet structure and intermolecular hydrogen bondformation between SF and PEG400. Thermogravimetric analysis showed that the initial thermal decomposition temperatureof the blend film was 170℃, which was 80℃ lower than that of SF (250℃) and 20℃ higher than that of PEG400 (150℃),and indicated a Strong interaction between two components of the blend. No crystalline peaks were observed in the X-raydiffraction curve of the blend film. Cell culture test showed that SF/PEG400 was a suitable substrate for the growth of humanumbilical vein endothelial cells (HUVEC).
