A comparative study for petroleum removal capacities of the bacterial consortia entrapped in sodium alginate,sodium alginate/poly(vinyl alcohol),and bushnell haas agar

The purpose of this study was to identify and compare the degradation efficiencies of free and entrapped bacterial consortia(Staphylococcus capitis CP053957.1 and Achromobacter marplatensis MT078618.1)to different polymers such as Sodium Alginate(SA),Sodium Alginate/Poly(Vinyl Alcohol)(SA/PVA),and Bushnell Haas Agar(BHA).In addition to SA and SA/PVA,which are cost-effective,non-toxic and have different functional groups,BHA,which is frequently encountered in laboratory-scale studies but has not been used as an entrapment material until now.Based on these,the polymers with different surface morphologies and chemical compositions were analyzed by SEM and FT-IR.While the petroleum removal efficiency was higher with the entrapped bacterial consortia than with the free one,BHA-entrapped bacterial consortium enhanced the petroleum removal more than SA and SA/PVA.Accordingly,the degradation rate of bacterial consortia entrapped with BHA was 2.039 day^(-1),SA/PVA was 1.560,SA was 0.993,the half-life period of BHA-entrapped bacterial consortia is quite low(t_(1/2)=0.339)compared with SA(t_(1/2)=0.444)and SA/PVA(t_(1/2)=0.697).The effects of the four main factors such as:amount of BHA(0.5,1,1.5,2,2.5,3 g),disc size(4,5,6,7,8 mm),inoculum concentration(1,2.5,5,7.5,10 mL),and incubation period on petroleum removal were also investigated.The maximum petroleum removal(94.5%)was obtained at≥2.5 mL of bacterial consortium entrapped in 2 g BHA with a 7 mm disc size at 168 h and the results were also confirmed by statistical analysis.Although a decrease was observed during the reuse of bacterial consortium entrapped in BHA,the petroleum removal was still above 50%at 10th cycle.Based on GC-MS analysis,the removal capacity of BHA-entrapped consortium was over 90%for short-chain n-alkanes and 80%for medium-chain n-alkanes.Overall,the obtained data are expected to provide a potential guideline in cleaning up the large-scale oil pollution in the future.Since there has been no similar study investigating petroleum removal with the bacterial consortia entrapped with BHA,this novel entrapment material can potentially be used in the treatment of petroleum pollution in advanced remediation studies.

Pretreatment of poly(acrylic acid) sodium by continuous diafiltration and time revolution of filtration potential

The pretreatment for the removal of small molecules from poly(acrylic acid) sodium (PAAS) solution by continuous diafiltration was investigated using ultrafiltration membrane. The effects of PAAS concentration, pH, trans-membrane pressure and pretreatment time on the permeate concentration and permeate flux were studied. The results show that the necessary pretreatment time (NPT) increases with PAAS concentration, decreases with TMP. The change trend of permeate flux with time is affected by pH. The permeate fluxes rapidly decrease from the start, and then increase gradually to stable values at pH 5.0, pH 7.0 and pH 9.3. However, it decreases gradually with time till a state value at pH 3.0 (iso-electric point, IEP). The removal of small molecules is easy at pH greater than iso-electric point (IEP). The change of filtration potential with time indicates the similar trend to that of permeation concentration, but the former is more convenient for indication of NPT.

Effect of Sodium Alginate Concentration on Membrane Strength and Permeating Property of Poly-l-arginine Group Microcapsule

A novel poly-/-arginine microcapsule was prepared due to its nutritional function and pharmacological efficacy. A high-voltage electrostatic droplet generator was used to make uniform microcapsules. The results show that the membrane strength and permeating property are both remarkably affected with the changes of sodium alginate concentration. With the sodium alginate concentration increasing, gel beads sizes increase from 233μm to 350μm, release ratio is also higher at the same time, but the membrane strength decreases.

Aggregation of Sodium Dodecyl Sulfonate in Poly-ethylene Glycol Aqueous Solutions Studied by Two-dimensional Nuclear Overhauser Enhancement Spectroscopy

Two-dimensional nuclear overhauser enhancement (2D NOESY)measurements show that sodium dodecyl sulfonate SDSN molecules co-aggregate with poly-ethylene glycol PEG in their aqueous solution at a concentration range of SDSN between the so-called co-aggregation concentration (cac) and the. Normal critical micellar concentration (cmc). SDSN micelles are formed when the cmc of SDSN is reached with PEG uniformly distributed in the interior.

POLYMERIZATION OF ETHYLENE METHYL PHOSPHATE IN THE PRESENCE OF SODIUM POLY(ETHYLENE GLYCOL)ATE

Poly(ethylene methyl phosphate)-poly(ethylene glycol)-poly(ethylene methyl phosphate) triblock copolymers carrying hydroxyl group at both chain ends were synthesized with sodium poly(ethylene glycol)ate as initiator. The effects of the factors such as solvent, amount of the initiator and reaction time were investigated. The copolymers were characterized by IR, H-1-NMR, H-1{P-31}-NMR, C-13-NMR, P-31{H-1}-NMR, and DSC. High molecular weight of the copolymer and high yield of the polymerization were achieved within 3 min at 25 degrees C. The polymerization process was studied by P-31{H-1}-NMR and transesterification was found during longer polymerization time.

INTERACTION OF POLY(SODIUM SULFODECYL METHACRYLATE)WITH CETYLTRIMETHYL AMMONIUM BROMIDE IN AQUEOUS SOLUTION

The interaction of poly(sodium sulfodecyl methacrylate) (PSSM) with cetyltrimethyl ammonium bromide (CTAB)was studied. It was found that the precipitate formed from PSSM and CTAB will be dissolved by excessive CTAB, resultingin the appearance of two maxima of the solution viscosity at the molar ratio (CTAB/-SO_3^-) of≈ 0.68 and≈1.30,respectively. The first one is related closely to the aggregation of polymer chains via CTAB molecules and the second oneshould be ascribed to the formation of the mixed micelles comprising surfactant and the polymer's hydrophobic chains. Theeffect of NaCl on the viscosity, the transmittance of the aqueous solution and the solubility of oil-soluble dye (dimethyl yellow) in the mixed system were also investigated.

Study of radiation-induced grafting of acrylic acid-sodium styrene sulfonate onto poly (tetrafluoroethylene-co-hexafluoropropylene) film

Poly(tetrafluoroethylene-co-hexafluoropropylene) (FEP) films were immersed in aqueous solution of acrylic acid (AA) and sodium styrene sulfonate (SSS), then irradiated by Co γ-rays at 25℃. The effects of reaction 60 time,absorbed dose, dose-rate, inhibitor and monomer concentration on the grafting yield were studied. Grafting yields of both AA and SSS onto FEP, respectively, increase with irradiation dose, but some saturation will appear at high dose and monomer concentration. The grafting yield increases with reaction time and then levels off. The graft- ing of SSS onto FEP is more difficult than the grafting of AA. The analysis of grafted membranes using DSC and FT-IR have been done.

Microanalyses of the hydroxyl-poly-calcium sodium phosphate coatings produced by ion beam assisted deposition

Thin calcium phosphate catings on titanium alloy substrates were prepared by Ar^+ ion beam assisted deposition(IBAD) from hydroxyl-poly-calcium sodium phosphate(HPPA) target.The coatings were analyzed by XRD,FTIR,XPS,These analyses revealed that the as-deposited films were amorphous or no apparent crystallinity.No distinct absorption band of the hydroxyl group was observed in FTIR spectra of the coatings but new absorption bands were presented for CO3^-2,The calcium to phosphorous ratio of these catings in different IBAD conditions varied from 0.46 to 3.36.

Separation Performance of Sodium Alginate/Poly(Vinyl Pyrrolidone)Membranes for Aqueous/Dimethylformamide Mixtures by Vapor Permeation and Vapor Permeation with Temperature Difference Methods

In this study sodium alginate (NaAlg)/poly (vinyl pyrrolidone) (PVP) blend membranes were prepared and crosslinked with CaCl2 (0.1 Molarity (M)) for the separation of aqueous/dimethylformamide (DMF) mixtures. Membranes were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and their performance was examined by varying experimental parameters such as feed composition (0 - 100 wt%), operating temperature (30℃ - 50℃) and membrane thickness (30 - 90 micrometer (μm)). Blending NaAlg with PVP, decreased separation factor whereas increased the permeation rate as the permeation temperature was increased in Vapor Permeation (VP) and Vapor Permeation with Temperature Difference (TDVP) methods. In the TDVP method, the separation factors increased and the permeation rates decreased as the temperature of the membrane surrounding is decreased. The highest separation factor of 60 was obtained in TDVP method for 90 wt% DMF concentration in the feed.

3种不同添加物对凡纳滨对虾生长、非特异免疫和抗病力的影响

为评估3种不同添加物对凡纳滨对虾(Litopenaeus vannamei)生长性能、非特异免疫反应及抗病力的影响,在基础饲料中分别添加了丁酸梭菌CBG01(Clostridium butyricum CBG01)活菌(CB组)、3%聚β-羟基丁酸酯(PHB组)和1%丁酸钠(BS组)来投喂对虾,对照组投喂基础饲料,养殖6周后测量对虾生长情况、检测血清非特异免疫指标和肝胰腺免疫相关基因表达水平,进行副溶血弧菌(Vibrio parahaemolyticus)攻毒实验。研究表明,CB组和PHB组对虾末体质量和特定生长率显著高于对照组(P<0.05),CB组最高,BS组与对照组差异不显著(P>0.05)。3个处理组对虾成活率和饲料效率均显著高于对照组(P<0.05)。3个处理组对虾血清中溶菌酶、过氧化物酶、超氧化物歧化酶活性以及总抗氧化能力显著高于对照组(P<0.05),CB组对虾血清中碱性磷酸酶、酸性磷酸酶、酚氧化酶活性显著高于对照组(P<0.05),PHB组对虾血清中碱性磷酸酶、酸性磷酸酶、总一氧化氮合酶活性显著高于对照组(P<0.05),BS组对虾血清中酸性磷酸酶活性显著高于对照组(P<0.05)。与对照组相比,CB组对虾肝胰腺中所有免疫相关基因(SOD、LZM、proPO、LGBP、HSP70、Imd、Toll、Relish、TOR、4E-BP、eIF4E1α、eIF4E2)相对表达量均显著上调(P<0.05),而PHB组的Toll基因以及BS组的Imd、Toll、Relish、eIF4E2基因相对表达量与对照组无显著差异(P>0.05)。副溶血弧菌攻毒实验表明,3个处理组对虾的累积死亡率均显著低于对照组(P<0.05)。研究结果表明,饲料中添加丁酸梭菌、PHB和丁酸钠均可不同程度地提高凡纳滨对虾的生长性能和非特异免疫能力,能够显著提高对虾对副溶血弧菌感染的抵抗力。总体比较,添加丁酸梭菌和PHB的作用效果基本相当,在一定程度上要优于添加丁酸钠。在饲料生产加工过程中,在难以添加丁酸梭菌活菌的情况下,建议适当添加聚β-羟基丁酸酯来替代丁酸梭菌。

聚苯乙烯磺酸钠与季鏻盐表面活性剂复合物的结构调控及其抗菌性能

发展非抗生素抗菌策略在减少医疗器械相关的细菌感染并降低对抗生素的过度依赖方面具有重大意义。对医疗器械表面进行功能化,赋予其抗菌性能是非抗生素抗菌所采用的常用方式。聚电解质-表面活性剂复合物的醇溶、水不溶的特点使其在构建抗菌涂层方面具有操作简单、不受器械结构限制的优点,有望应用于各种医疗器械的表面功能化。本文通过构建不同烷基链长季鏻盐表面活性剂与聚苯乙烯磺酸钠聚阴离子复合物,系统研究了复合物在水溶液中的组装行为以及所制备复合物涂层的抗菌性能与表面活性剂组分疏水碳链长度之间的内在关联。通过浊度、等温滴定量热法等测试手段对聚苯乙烯磺酸钠与3种三丁基烷基季鏻盐表面活性剂在水中的组装过程进行了分析,结果表明增加表面活性剂烷基链长将促进聚电解质-表面活性剂复合物形成。通过在常用医疗器械材料表面构建复合物涂层,对比了3种复合物对两种模型菌种的抗菌性能。对于以金黄色葡萄球菌为代表的革兰氏阳性菌,所制备的3种复合物涂层的杀菌率分别为42.9%、99.97%和99.99%,随着表面活性剂烷基链越长而提高;对于大肠杆菌为代表的革兰氏阴性菌,随着表面活性剂烷基链长度增加,单独季鏻盐表面活性剂的抗菌性能增强,而其对应复合物涂层的抗菌性能有所下降,杀菌率分别为99.3%、92.3%和64.8%。以上结果表明,通过优化结构,此类聚电解质-表面活性剂复合物涂层有望用于减少医疗器械相关感染。

含左旋乳酸-乙二醇共聚物微球的交联透明质酸钠凝胶用于中面部年轻化的临床效果观察

目的:探究含左旋乳酸-乙二醇共聚物微球的交联透明质酸钠凝胶用于中面部年轻化的有效性与安全性。方法:回顾性分析13例已接受含左旋乳酸-乙二醇共聚物微球的交联透明质酸钠凝胶进行中面部注射填充的就医者病例资料,术后第1、3、6、12个月进行随访观察,评估患者鼻唇沟皱纹、中面部容量缺失、中面部整体美学改善效果、就医者满意度及不良反应。结果:本组13例就医者,10例进行了面颊部和鼻唇沟双部位注射填充,3例只进行了鼻唇沟注射填充,所有就医者均接受单次治疗,左侧鼻唇沟平均注射剂量为(0.69±0.45)ml,右侧鼻唇沟平均注射剂量为(0.69±0.40)ml;左侧面颊部平均注射剂量为(0.46±0.23)ml,右侧面颊部平均注射剂量为(0.56±0.22)ml。术后第1个月和第3个月,就医者鼻唇沟皱纹、中面部容量缺失、中面部整体美学改善率及就医者满意度均为100%;术后第6个月和第12个月,就医者以上各指标改善率均有所下降。所有就医者术后均未发生严重不良反应。结论:本研究应用含左旋乳酸-乙二醇共聚物微球的交联透明质酸钠凝胶用于中面部年轻化的临床效果与安全性良好,值得临床推广应用。

GRAFTING OF POLY(SODIUM STYRENESULFONATE) ONTO GOLD SURFACE FROM NaNO_3 SOLUTION

The chemical grafting of thiol terminated poly（sodium styrenesulfonate） （HS-PSSS） chains from sodium nitrate （NaNO3） salt solution to a gold surface was investigated with a quartz crystal microbalance with dissipation monitoring （QCM-D） in different salt concentrations, It was found that at low salt concentration grafting density of HS-PSSS was low and the grafted chains adopted a mushroom conformation. With the increase of salt concentration polyelectrolyte chains underwent a transition toward coiled state due to reduction of electrostatic repulsion and as a result more chains were grafted on the surface, When the grafting density reached a certain limit after which further grafting caused repulsion between already grafted HS-PSSS chains and as a result grafted chains adopted a brush conformation.

基于聚(3,4-亚乙基二氧噻吩)掺杂聚(苯乙烯磺酸)的柔性织物电极的研究进展

介绍了基于聚(3,4-亚乙基二氧噻吩)导电聚合物掺杂聚(苯乙烯磺酸)的柔性织物电极的常用制备方法,评估了各方法的优缺点。概述了基于聚(3,4-亚乙基二氧噻吩)掺杂聚(苯乙烯磺酸)的织物电极在能源存储、心电图监测、电子传感和电磁屏蔽等领域的应用。此外,还分析了织物基电极在能源储存方面所面临的挑战,并进行了展望。

柔性PEDOT:PSS修饰Te纳米棒/PEDOT纳米线复合薄膜的制备及热电性能

通过改进的自组装胶束软模板法和湿化学法分别成功合成了聚(3,4-乙烯二氧噻吩)纳米线(PEDOT NW)和聚(3,4-乙烯二氧噻吩):聚苯乙烯磺酸(PEDOT:PSS)修饰的Te纳米棒(PC-Te),采用真空抽滤工艺制备了柔性自支撑PC-Te/PEDOT NW复合薄膜。通过透射电子显微镜等对PC-Te的显微结构进行表征,研究了PC-Te的添加量对复合薄膜的热电性能的影响规律。随着PC-Te含量的增加,复合薄膜的Seebeck系数增大,电导率减小,PC-Te含量为70%时,复合薄膜的功率因子的最大值达到47.4μW/mK^(2)(380 K)。该薄膜具有良好的柔性,弯曲500次后,其电阻变化率为9.2%。

Synthesis of poly(m-phenylenediamine) with improved properties and superior prospect for Cr(VI) removal

Chemically oxidative polymerization of m-phenylenediamine was improved through adding the weak alkaline, Na2CO3. Results show that the poly （m-phenylenediamine） （PmPD） possesses a weak solubility in acidic solution according to total organic carbon （TOC） that the TOC is less than 8 mg/L, which is much lower than the discharge standard （20 mg/L）. The TOC of the PmPD synthesized with NaOH can be as high as 120.9 mg/L. This very weak solubility of PmPD synthesized with Na2CO3 facilitates its application in water purification. The oxidation state of PmPD is decreased and the yield is increased with a maximum of 84%, promoting the concentration of Na2CO3 in the synthesis. Moreover, the Cr（VI） performance of PmPD was marvelously enhanced with Na2CO3 to improve the synthesis. The largest Cr（VI） adsorbance can reach as high as 666.8 mg/g, which is far more than the performance of other common adsorbents.

Development of Polycaprolactone/Poly(Vinyl Alcohol)/Clay Microparticles by Spray Drying

In this study, nanostructured microparticles was developed with polycaprolactone (PCL), poly(vinyl alcohol) (PVAL) and nanoparticles of the commercial sodium clay NT-25®by using the spray drying technique. The systems obtained were characterized by Nuclear Magnetic Resonance (NMR), Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Dynamic Laser Light Scattering (DLS) and Differential Scanning Calorimetry (DSC). The NMR 13C and FTIR techniques showed that both polymers were present in the microparticles and the DSC analysis revealed a small variation in the glass transition temperature of the PCL. The XRD and SEM analyses showed that the microparticles produced were amorphous and had a concave morphology. The NT-25 nanoload reduced the microparticles’ size due to the multiple interactions formed in the hybrid nanocomposite material. Therefore, it was possible to develop microparticles by using biodegradable and biocompatible polymers, with different polarities, allowing the incorporation of hydrophilic and hydrophobic materials and enabling the inclusion of otherwise incompatible materials in the same system.

聚电解质盐三聚磷酸钠对聚乙烯醇凝胶的Hofmeister效应研究

聚乙烯醇(PVA)物理微晶水凝胶由于化学稳定性好、无毒、生物相容性好等特点,近年来成为物理微晶凝胶研究的模板聚合物。但其力学性能较差,如何提高凝胶的力学性能,拓展其应用范围一直是水凝胶领域的研究难点和热点。Hofmeister盐析效应是提升凝胶力学性能一种高效、绿色的制备手段,但之前的研究多集中在小分子无机盐对凝胶性能的影响,对聚电解质盐的盐析效应鲜见报道。本文研究了三聚磷酸钠(STPP)聚电解质对PVA凝胶性能的影响,系统比较了其与氯化钠及氢氧化钠处理的PVA凝胶;重点从微观形态和结晶结构两方面研究了聚电解质盐与小分子盐引起PVA凝胶的微观结构差异,并分析了结构与性能间的响应关系。聚电解质STPP处理的PVA凝胶薄膜应力达到了23 MPa,应变高达1 100%,断裂韧性(110 MJ/cm^(3)),比纯水凝胶提升55倍之多,并兼具良好的阻燃性能。

聚丙烯酸钠对聚丁二酸丁二醇酯水解性能的影响

采用熔融共混法制备了聚丁二酸丁二醇酯/聚丙烯酸钠(PBS/PAAS)共混物,探究PAAS对PBS水解性能的影响。通过吸水率和失重率分析了共混物的水解性能,利用拉力试验机、差示扫描量热、扫描电镜和X射线衍射仪等对共混物的力学性能及水解前后结晶性能、微观形貌和结晶结构进行表征。结果表明,随PAAS用量的增大,共混物基体孔洞增多,水解性能有较大提升。当PAAS用量为100份时,共混物的吸水率最高为110.1%,水解失重率为44.6%;加入PAAS后,共混物的拉伸强度、屈服强度和断裂伸长率降低,弹性模量升高。PAAS可以提高共混物的结晶温度,但对熔融温度影响不大,随水解时间的延长,共混物的熔融温度没有发生变化,但PBS组分的熔融焓变化明显。同时,水解过程中共混物中PBS的晶型没有变化。

脂肪醇聚氧乙烯醚的原料指标对AES中二噁烷含量的影响

为研究脂肪醇聚氧乙烯醚原料(简称AEO)对脂肪醇聚氧乙烯醚硫酸钠(AES)中二噁烷的影响,在嘉兴赞宇工厂的膜式磺化器装置上,考察了AEO中水分含量、PEG含量、环氧乙烷含量对AES中二噁烷含量的影响。结果表明:随着AEO中水分、PEG、环氧乙烷含量的增加,使AES中二噁烷增加,特别是环氧乙烷的影响最为显著,并且环氧乙烷的增加与二噁烷含量的增加呈非线性关系。文中从反应机理上进行了推导和解释。