通过低温等离子体技术对聚乙二醇双丙烯酸酯(PEGDA)/甲基丙烯酸β-羟乙酯(HEMA)共聚物水凝胶生物材料进行表面改性,以骨髓基质干细胞(BMSc)为细胞模型,考察了细胞在等离子体表面改性前后的水凝胶材料的黏附和增值行为.材料的表面性能通...通过低温等离子体技术对聚乙二醇双丙烯酸酯(PEGDA)/甲基丙烯酸β-羟乙酯(HEMA)共聚物水凝胶生物材料进行表面改性,以骨髓基质干细胞(BMSc)为细胞模型,考察了细胞在等离子体表面改性前后的水凝胶材料的黏附和增值行为.材料的表面性能通过X射线光电子能谱、接触角和扫描电镜进行表征.研究结果表明,材料表面经氩等离子体处理后,其亲水性得到较大的改善,表面自由能由45.9 m J/m2增加到70.3 m J/m2;体外实验结果证明,BMSc在等离子体处理后材料表面培养24 h后出现明显细胞核,168 h细胞融合成片,通过等离子体处理方法有利于细胞在水凝胶材料表面的黏附和增殖.展开更多
To investigate the effects of polyethylene glycol cross-linking on the mechanical properties, 80 porcine aortic valves were harvested, decellularized, and introduced with sulflaydryl. Then the valves were randomly ass...To investigate the effects of polyethylene glycol cross-linking on the mechanical properties, 80 porcine aortic valves were harvested, decellularized, and introduced with sulflaydryl. Then the valves were randomly assigned into 5 experimental groups and 1 control group （n=16）. For the valves in those experimental groups, branched polyethylene glycol diacrylate （PEG） of 5 different molecular weights （3.4, 8, 12, 20, 40 kDa） were synthesized and cross-linked with them respectively. The efficiency of the cross-linking was determined by measuring the amount of residual thiol group and the mechanical properties of the cross-linked valve leaflets were assessed by uni-axial planar tensile testing. The efficiency of the PEG 20 kDa group was 70.72±2.33%, obviously superior to that of the other groups （p〈0.05）. Tensile test proved that branched PEG cross-linking can significantly enhance the mechanical behaviors of the deeellularized valve leaflet and the Young＇s modulus of each group was positively correlated with the molecular weight of PEG. It was concluded that branched PEG with the molecular weight of 20 kDa can effectively cross-link the decellularized porcine aortic valves and improve their mechanical properties, which makes it a promising cross-linker that can be used in the modification of decellularized tissue engineering valves.展开更多
Polyethylene glycol diacrylates(PEGDA)monomers, which contain double bond on the terminal group of polyethylene glycol(PEG)and can be used in synthesis of UV-curable solid polymer electrolytes (SPE), were prepared thr...Polyethylene glycol diacrylates(PEGDA)monomers, which contain double bond on the terminal group of polyethylene glycol(PEG)and can be used in synthesis of UV-curable solid polymer electrolytes (SPE), were prepared through esterification of PEG and acrylic acid.A cured conductive polymer film consisting of lithium salt was then obtained by irradiation with ultraviolet rays.The factors affecting film-forming,photosensitivity and conductance were studied.From experiments, while Li/O=1/6 and n =18,the ionic conductivity of the SPE membrane could reach 10 -5 ?S·cm -1 at room temperature and its performance was relatively good.展开更多
Background:Targeted T-cell therapy has emerged as a promising strategy for the treatment of hematological malignancies.However,its application to solid tumors presents significant challenges due to the limited accessi...Background:Targeted T-cell therapy has emerged as a promising strategy for the treatment of hematological malignancies.However,its application to solid tumors presents significant challenges due to the limited accessibility and heterogeneity.Localized delivery of tumor-specific T-cells using biomaterials has shown promise,however,procedures required for genetic modification and generation of a sufficient number of tumor-specific T-cells ex vivo remain major obstacles due to cost and time constraints.Methods:Polyethylene glycol(PEG)-based three-dimensional(3D)scaffolds were developed and conjugated with positively charged poly-L-lysine(PLL)using carbamide chemistry for efficient loading of lentiviruses(LVs)carrying tumor antigen-specific T-cell receptors(TCRs).The physical and biological properties of the scaffold were extensively characterized.Further,the scaffold loaded with OVA-TCR LVs was implanted in B16F10 cells expressing ovalbumin(B16-OVA)tumor model to evaluate the anti-tumor response and the presence of transduced T-cells.Results:Our findings demonstrate that the scaffolds do not induce any systemic inflammation upon subcutaneous implantation and effectively recruit T-cells to the site.In B16-OVA melanoma tumor-bearing mice,the scaffolds efficiently transduce host T-cells with OVA-specific TCRs.These genetically modified T-cells exhibit homing capability towards the tumor and secondary lymphoid organs,resulting in a significant reduction of tumor size and systemic increase in anti-tumor cytokines.Immune cell profiling revealed a significantly high percentage of transduced T-cells and a notable reduction in suppressor immune cells within the tumors of mice implanted with these scaffolds.Conclusions:Our scaffold-based T-cell therapy presents an innovative in situ localized approach for programming T-cells to target solid tumors.This approach offers a viable alternative to in vitro manipulation of T-cells,circumventing the need for large-scale in vitro generation and culture of tumor-specific T-cells.It offers an off-the-shelf alternative that facilitates the use of host cells instead of allogeneic cells,thereby,overcoming a major hurdle.展开更多
文摘通过低温等离子体技术对聚乙二醇双丙烯酸酯(PEGDA)/甲基丙烯酸β-羟乙酯(HEMA)共聚物水凝胶生物材料进行表面改性,以骨髓基质干细胞(BMSc)为细胞模型,考察了细胞在等离子体表面改性前后的水凝胶材料的黏附和增值行为.材料的表面性能通过X射线光电子能谱、接触角和扫描电镜进行表征.研究结果表明,材料表面经氩等离子体处理后,其亲水性得到较大的改善,表面自由能由45.9 m J/m2增加到70.3 m J/m2;体外实验结果证明,BMSc在等离子体处理后材料表面培养24 h后出现明显细胞核,168 h细胞融合成片,通过等离子体处理方法有利于细胞在水凝胶材料表面的黏附和增殖.
基金funded by the National High-Technology Research and Development Program of China(863 Program)(No.2009AA03Z420)the National Natural Science Foundation of China(Nos.30872540,81400290)
文摘To investigate the effects of polyethylene glycol cross-linking on the mechanical properties, 80 porcine aortic valves were harvested, decellularized, and introduced with sulflaydryl. Then the valves were randomly assigned into 5 experimental groups and 1 control group （n=16）. For the valves in those experimental groups, branched polyethylene glycol diacrylate （PEG） of 5 different molecular weights （3.4, 8, 12, 20, 40 kDa） were synthesized and cross-linked with them respectively. The efficiency of the cross-linking was determined by measuring the amount of residual thiol group and the mechanical properties of the cross-linked valve leaflets were assessed by uni-axial planar tensile testing. The efficiency of the PEG 20 kDa group was 70.72±2.33%, obviously superior to that of the other groups （p〈0.05）. Tensile test proved that branched PEG cross-linking can significantly enhance the mechanical behaviors of the deeellularized valve leaflet and the Young＇s modulus of each group was positively correlated with the molecular weight of PEG. It was concluded that branched PEG with the molecular weight of 20 kDa can effectively cross-link the decellularized porcine aortic valves and improve their mechanical properties, which makes it a promising cross-linker that can be used in the modification of decellularized tissue engineering valves.
文摘Polyethylene glycol diacrylates(PEGDA)monomers, which contain double bond on the terminal group of polyethylene glycol(PEG)and can be used in synthesis of UV-curable solid polymer electrolytes (SPE), were prepared through esterification of PEG and acrylic acid.A cured conductive polymer film consisting of lithium salt was then obtained by irradiation with ultraviolet rays.The factors affecting film-forming,photosensitivity and conductance were studied.From experiments, while Li/O=1/6 and n =18,the ionic conductivity of the SPE membrane could reach 10 -5 ?S·cm -1 at room temperature and its performance was relatively good.
基金Department of Biotechnology(DBT,Govt of India)(BT/PR31315/MED/32/667/2019)DBT along with Wadhwani Research Center for Bioengineering,IIT Bombay(BT/INF/22/SP23026/2017)Department of Biotechnology(DBT,Govt of India)(BT/INF/22/SP17358/2016).
文摘Background:Targeted T-cell therapy has emerged as a promising strategy for the treatment of hematological malignancies.However,its application to solid tumors presents significant challenges due to the limited accessibility and heterogeneity.Localized delivery of tumor-specific T-cells using biomaterials has shown promise,however,procedures required for genetic modification and generation of a sufficient number of tumor-specific T-cells ex vivo remain major obstacles due to cost and time constraints.Methods:Polyethylene glycol(PEG)-based three-dimensional(3D)scaffolds were developed and conjugated with positively charged poly-L-lysine(PLL)using carbamide chemistry for efficient loading of lentiviruses(LVs)carrying tumor antigen-specific T-cell receptors(TCRs).The physical and biological properties of the scaffold were extensively characterized.Further,the scaffold loaded with OVA-TCR LVs was implanted in B16F10 cells expressing ovalbumin(B16-OVA)tumor model to evaluate the anti-tumor response and the presence of transduced T-cells.Results:Our findings demonstrate that the scaffolds do not induce any systemic inflammation upon subcutaneous implantation and effectively recruit T-cells to the site.In B16-OVA melanoma tumor-bearing mice,the scaffolds efficiently transduce host T-cells with OVA-specific TCRs.These genetically modified T-cells exhibit homing capability towards the tumor and secondary lymphoid organs,resulting in a significant reduction of tumor size and systemic increase in anti-tumor cytokines.Immune cell profiling revealed a significantly high percentage of transduced T-cells and a notable reduction in suppressor immune cells within the tumors of mice implanted with these scaffolds.Conclusions:Our scaffold-based T-cell therapy presents an innovative in situ localized approach for programming T-cells to target solid tumors.This approach offers a viable alternative to in vitro manipulation of T-cells,circumventing the need for large-scale in vitro generation and culture of tumor-specific T-cells.It offers an off-the-shelf alternative that facilitates the use of host cells instead of allogeneic cells,thereby,overcoming a major hurdle.
