Excess extracellular glutamate leads to excitotoxicity,which induces neuronal death through the overactivation of N-methyl-D-aspartate receptors(NMDARs).Excitotoxicity is thought to be closely related to various acute...Excess extracellular glutamate leads to excitotoxicity,which induces neuronal death through the overactivation of N-methyl-D-aspartate receptors(NMDARs).Excitotoxicity is thought to be closely related to various acute and chronic neurological disorders,such as stroke and Alzheimer’s disease.Polygalasaponin F(PGSF)is a triterpenoid saponin monomer that can be isolated from Polygala japonica,and has been reported to protect cells against apoptosis.To investigate the mechanisms underlying the neuroprotective effects of PGSF against glutamateinduced cytotoxicity,PGSF-pretreated hippocampal neurons were exposed to glutamate for 24 hours.The results demonstrated that PGSF inhibited glutamate-induced hippocampal neuron death in a concentration-dependent manner and reduced glutamate-induced Ca^(2+)overload in the cultured neurons.In addition,PGSF partially blocked the excess activity of NMDARs,inhibited both the downregulation of NMDAR subunit NR2A expression and the upregulation of NMDAR subunit NR2B expression,and upregulated the expression of phosphorylated cyclic adenosine monophosphate-responsive element-binding protein and brain-derived neurotrophic factor.These findings suggest that PGSF protects cultured hippocampal neurons against glutamate-induced cytotoxicity by regulating NMDARs.The study was approved by the Institutional Animal Care Committee of Nanchang University(approval No.2017-0006)on December 29,2017.展开更多
目的建立测定瓜子金皂苷己的含量的方法,为瓜子金药材质量控制提供依据。方法采用HPLC-ELSD法;色谱柱：SHISEIDO Capcell Pak C18柱;流动相：乙腈-水（30：70）,等度洗脱;流速：1.0 ml/min;柱温：25℃,检测器：蒸发光散射检测器。结果...目的建立测定瓜子金皂苷己的含量的方法,为瓜子金药材质量控制提供依据。方法采用HPLC-ELSD法;色谱柱：SHISEIDO Capcell Pak C18柱;流动相：乙腈-水（30：70）,等度洗脱;流速：1.0 ml/min;柱温：25℃,检测器：蒸发光散射检测器。结果瓜子金中瓜子金皂苷己获得良好分离,在0.346~3.460μg范围内呈现良好线性关系;精密度试验RSD为1.64%;重现性试验RSD为2.12%;加样回收率为99.85%。结论建立了HPLC-ELSD法测定瓜子金皂苷己的含量,方法简单可行。展开更多
基金supported by the National Natural Science Foundation of China,Nos.31971035(to BML),31771182(to BML),81471116(to BML)the Natural Science Foundation of Jiangxi Province of China,Nos.20171BAB204019(to CS),20192ACB20022(to CS)。
文摘Excess extracellular glutamate leads to excitotoxicity,which induces neuronal death through the overactivation of N-methyl-D-aspartate receptors(NMDARs).Excitotoxicity is thought to be closely related to various acute and chronic neurological disorders,such as stroke and Alzheimer’s disease.Polygalasaponin F(PGSF)is a triterpenoid saponin monomer that can be isolated from Polygala japonica,and has been reported to protect cells against apoptosis.To investigate the mechanisms underlying the neuroprotective effects of PGSF against glutamateinduced cytotoxicity,PGSF-pretreated hippocampal neurons were exposed to glutamate for 24 hours.The results demonstrated that PGSF inhibited glutamate-induced hippocampal neuron death in a concentration-dependent manner and reduced glutamate-induced Ca^(2+)overload in the cultured neurons.In addition,PGSF partially blocked the excess activity of NMDARs,inhibited both the downregulation of NMDAR subunit NR2A expression and the upregulation of NMDAR subunit NR2B expression,and upregulated the expression of phosphorylated cyclic adenosine monophosphate-responsive element-binding protein and brain-derived neurotrophic factor.These findings suggest that PGSF protects cultured hippocampal neurons against glutamate-induced cytotoxicity by regulating NMDARs.The study was approved by the Institutional Animal Care Committee of Nanchang University(approval No.2017-0006)on December 29,2017.
文摘目的建立测定瓜子金皂苷己的含量的方法,为瓜子金药材质量控制提供依据。方法采用HPLC-ELSD法;色谱柱：SHISEIDO Capcell Pak C18柱;流动相：乙腈-水（30：70）,等度洗脱;流速：1.0 ml/min;柱温：25℃,检测器：蒸发光散射检测器。结果瓜子金中瓜子金皂苷己获得良好分离,在0.346~3.460μg范围内呈现良好线性关系;精密度试验RSD为1.64%;重现性试验RSD为2.12%;加样回收率为99.85%。结论建立了HPLC-ELSD法测定瓜子金皂苷己的含量,方法简单可行。
基金Project supported by the Medicinal Science and Technology Research Project(No.BWS11J052)the Ministry of Science and Technology of China(No.2012ZX09J12201)the Department of Science and Technology of Xinjiang Uygur Autonomous Region,China(No.201491174)
