Effect of Surface Modification on Microbiol Polyhydroxyalkanoate Films on Biocompatibility

The purpose of this study was to investigate in vitro biocompatibility of a new type of polymer, polyhydroxybutyrate co hexanoate (PHBHHx). The hydrophilicity and biocompatibility were studied with two kinds of enzymes, amylase BAN480L and lipase Novozym388. The degree of hydrophilicity was observed using contact angle measurements. In vitro biocompatibility evaluations were carried out by direct incubation of mouse fibroblast cell line L929 on the polyhydroxyalkanoate (PHA) films. The samples treated with BAN480L showed that the PHA biocompatibility increased while the hydrophilicity decreased. Relative to untreated samples, the number of cells on the Novozym388 modified PHBHHx significant decrease as the hydrophilicity also decreased. The results indicated that other surface characteristics besides hydrophilicity influence the biocompatibility of PHBHHx films.

生物可降解塑料——聚羟基脂肪酸酯(PHA)的生产技术研究

清华大学和中国科学院微生物研究所合作超额完成了可生物降解塑料专题的攻关任务,该专题包括用废糖蜜为原料生产可生物降解塑料聚羟基丁酸酯(PHB)、基因工程菌生产可生物降解塑料PHB、用水解淀粉为原料生产可生物降解塑料PHB及其共聚物PH-BV、可生物降解塑料PHB的改性和应用研究等4个子专题。并在此基础上实现了世界上首次规模化生产第三代PHA——羟基丁酸共聚羟基己酸酯(PHBHHx),为我国的生物可降解塑料工业化研究开辟了广阔的前景。