Sixty-five cases of herpes zoster were randomly divided into the He-Ne laser group(33 cases) and polyinosinic acid group (32 cases). Type JI He-Ne laser universal treatment apparatuswas used to irradiate injured cutan...Sixty-five cases of herpes zoster were randomly divided into the He-Ne laser group(33 cases) and polyinosinic acid group (32 cases). Type JI He-Ne laser universal treatment apparatuswas used to irradiate injured cutaneous regions and to radiate Hegu (LI 4) and Zusanli (ST 36) pointswith photoconductive fibers once every day in the He-Ne laser group; 2 ml of polyinosinic acid was in-tramuscularly injected once every other day in the other group. Of the 65 cases 63 were cured, andthe two cases with sequela of neuralgia were all in the latter group. Pain was disappeared and scabswere formed respectively after 1. 48 and 5. 76 days of treatment with He-Ne laser therapy, and after10. 5 and 10. 4 days with polyinosinic acid treatment. The differences of therapeutic effects betweenthe two groups were noticeably significant (P【0. 01 ).展开更多
Background Excessive iodine intake and viral infection are recognized as both critical factors associated with autoimmune thyroid diseases. Toll-like receptors （TLRs） have been reported to play an important role in ...Background Excessive iodine intake and viral infection are recognized as both critical factors associated with autoimmune thyroid diseases. Toll-like receptors （TLRs） have been reported to play an important role in autoimmune and inflammatory disorders. In this study, we aimed to clarify the possible mechanism of TLR3 involved in polyinosine- polycytidylic acid （poly（I：C）） promoting excessive iodine intake induced thyroiditis in non-obese diabetic （NOD） mice. Methods Both NOD and BALB/c mice were randomly assigned to four groups： control group （n=5）, high iodine intake （HI） group （n=7）, poly（I：C） group （n=7） and combination of excessive iodine and poly（l：C） injection （HIP） group （n=7）. After 8 weeks, mice were weighed and blood samples were collected. All the mice were sacrificed before dissection of spleen and thyroid gland. Then, thyroid histology, thyroid secreted hormone, expression of CD3＋ cells and TLR3 as well as inflammatory mRNA level were evaluated. Results Both NOD and BALB/c mice from HI and HIP group represented goiter and increasing thyroid relative weight. Thyroid histology evidence indicated that only HIP group of NOD mice showed severe thyroiditis with lymphocytes infiltration in majority of thyroid tissue, severe damage of follicles and general fibrosis. Immunofiuorescence staining results displayed a large number of CD3~ cells in HIP NOD mice. Real-time polymerase chain reaction （PCR） results suggested interferon （IFN）-a increased over 30 folds and IFN-y expression was doubled compared with control group, but interleukin （IL）-4 remained unchanged in HIP group of NOD mice thyroid. Meanwhile, over one third decrease of blood total thyroxine （TT4） and increased thyroid-stimulating hormone （TSH） was observed in HIP group of NOD mice. Only HIP group of NOD mice represented significantly elevation of TLR3 expression. Conclusion Poly（l：C） enhanced excessive dietary iodine induced thyroiditis in NOD mice through increasing TLR3 mediated inflammation.展开更多
文摘Sixty-five cases of herpes zoster were randomly divided into the He-Ne laser group(33 cases) and polyinosinic acid group (32 cases). Type JI He-Ne laser universal treatment apparatuswas used to irradiate injured cutaneous regions and to radiate Hegu (LI 4) and Zusanli (ST 36) pointswith photoconductive fibers once every day in the He-Ne laser group; 2 ml of polyinosinic acid was in-tramuscularly injected once every other day in the other group. Of the 65 cases 63 were cured, andthe two cases with sequela of neuralgia were all in the latter group. Pain was disappeared and scabswere formed respectively after 1. 48 and 5. 76 days of treatment with He-Ne laser therapy, and after10. 5 and 10. 4 days with polyinosinic acid treatment. The differences of therapeutic effects betweenthe two groups were noticeably significant (P【0. 01 ).
基金This work was supported by the grants from the Science and Technology Council of Tianjin （No. 05YFGDSF02700） for Scientific Research and the National Natural Science Foundation of China （No. 30972559）.
文摘Background Excessive iodine intake and viral infection are recognized as both critical factors associated with autoimmune thyroid diseases. Toll-like receptors （TLRs） have been reported to play an important role in autoimmune and inflammatory disorders. In this study, we aimed to clarify the possible mechanism of TLR3 involved in polyinosine- polycytidylic acid （poly（I：C）） promoting excessive iodine intake induced thyroiditis in non-obese diabetic （NOD） mice. Methods Both NOD and BALB/c mice were randomly assigned to four groups： control group （n=5）, high iodine intake （HI） group （n=7）, poly（I：C） group （n=7） and combination of excessive iodine and poly（l：C） injection （HIP） group （n=7）. After 8 weeks, mice were weighed and blood samples were collected. All the mice were sacrificed before dissection of spleen and thyroid gland. Then, thyroid histology, thyroid secreted hormone, expression of CD3＋ cells and TLR3 as well as inflammatory mRNA level were evaluated. Results Both NOD and BALB/c mice from HI and HIP group represented goiter and increasing thyroid relative weight. Thyroid histology evidence indicated that only HIP group of NOD mice showed severe thyroiditis with lymphocytes infiltration in majority of thyroid tissue, severe damage of follicles and general fibrosis. Immunofiuorescence staining results displayed a large number of CD3~ cells in HIP NOD mice. Real-time polymerase chain reaction （PCR） results suggested interferon （IFN）-a increased over 30 folds and IFN-y expression was doubled compared with control group, but interleukin （IL）-4 remained unchanged in HIP group of NOD mice thyroid. Meanwhile, over one third decrease of blood total thyroxine （TT4） and increased thyroid-stimulating hormone （TSH） was observed in HIP group of NOD mice. Only HIP group of NOD mice represented significantly elevation of TLR3 expression. Conclusion Poly（l：C） enhanced excessive dietary iodine induced thyroiditis in NOD mice through increasing TLR3 mediated inflammation.
