A new bovine serum albumin （BSA） surface imprinting method was developed by the incorporation of quantum dots （QDs） into molecularly imprinted polymers （MIP）, which can offer shape selectivity. Preparation and a...A new bovine serum albumin （BSA） surface imprinting method was developed by the incorporation of quantum dots （QDs） into molecularly imprinted polymers （MIP）, which can offer shape selectivity. Preparation and adsorption conditions were optimized. Physical appearance of the QDs and QDs-MIP particles was illustrated by scanning electron microscope images. Photoluminescence emission of CdS was quenched when rebinding of the template.The quenching of photoluminescence emissions is presumably due to the fluorescence resonance energy transfer between quantum dots and BSA template molecules. The adsorption is compiled with Langmuir isotherm, and chemical adsorption is the rate-controlling step.The maximum adsorption capacity could reach 226.0 mg/g, which is 142.4 mg/g larger than that of undoped BSA MIP. This study demonstrates the validity of QDs coupled with MIP technology for analyzing BSA.展开更多
In this paper, a surface plasmon resonance （SPR） sensor chip for detection of bovine serum album （BSA） was prepared by electropolymerization of 3-aminophenylboronic acid （3-APBA） based on molecularly imprinted p...In this paper, a surface plasmon resonance （SPR） sensor chip for detection of bovine serum album （BSA） was prepared by electropolymerization of 3-aminophenylboronic acid （3-APBA） based on molecularly imprinted polymer （MIP） technique. The surface morphology of MIP and non-imprinted （NIP） films were characterized by scanning electroscopy （SEM）. SEM images exhibited nanoscale cavities formed on the MIP films surface homogeneously due to the removal of BSA templates. The effects of pH, ion strength of rebinding BSA, the specific binding and selective recognition were studied for MIP films. Results indicated that the BSA-imprinted films exhibited a good adsorption of template protein （0.02-0.8 mg/mL） in 0.05 mol/L sodium phosphate buffer at pH 5.0 with the limit of detection （LOD） of 0.02 mg/mL.展开更多
基金This work was supported by the National Natural Science Foundation of China （No.20405013）.
文摘A new bovine serum albumin （BSA） surface imprinting method was developed by the incorporation of quantum dots （QDs） into molecularly imprinted polymers （MIP）, which can offer shape selectivity. Preparation and adsorption conditions were optimized. Physical appearance of the QDs and QDs-MIP particles was illustrated by scanning electron microscope images. Photoluminescence emission of CdS was quenched when rebinding of the template.The quenching of photoluminescence emissions is presumably due to the fluorescence resonance energy transfer between quantum dots and BSA template molecules. The adsorption is compiled with Langmuir isotherm, and chemical adsorption is the rate-controlling step.The maximum adsorption capacity could reach 226.0 mg/g, which is 142.4 mg/g larger than that of undoped BSA MIP. This study demonstrates the validity of QDs coupled with MIP technology for analyzing BSA.
基金the National Natural Science Foundation of China(No.20771015)the 111 Project(No.B0712)for the funding
文摘In this paper, a surface plasmon resonance （SPR） sensor chip for detection of bovine serum album （BSA） was prepared by electropolymerization of 3-aminophenylboronic acid （3-APBA） based on molecularly imprinted polymer （MIP） technique. The surface morphology of MIP and non-imprinted （NIP） films were characterized by scanning electroscopy （SEM）. SEM images exhibited nanoscale cavities formed on the MIP films surface homogeneously due to the removal of BSA templates. The effects of pH, ion strength of rebinding BSA, the specific binding and selective recognition were studied for MIP films. Results indicated that the BSA-imprinted films exhibited a good adsorption of template protein （0.02-0.8 mg/mL） in 0.05 mol/L sodium phosphate buffer at pH 5.0 with the limit of detection （LOD） of 0.02 mg/mL.
