A polymerized liposome （PLS） was prepared using a synthesized phospholipid with a diacetylene moiety.The precipitable property of the PLS on salt additions,ligand immobilization,affinity adsorption kinetics and adso...A polymerized liposome （PLS） was prepared using a synthesized phospholipid with a diacetylene moiety.The precipitable property of the PLS on salt additions,ligand immobilization,affinity adsorption kinetics and adsorption isotherms were studied.Trypsin was purified by affinity precipitation from a crude pancreatic extract.The specific activity reached about 13000 U.mg－1. These results indicate that the PLS is a potential ligand carrier for affinity precipitation of proteins.展开更多
文摘A polymerized liposome （PLS） was prepared using a synthesized phospholipid with a diacetylene moiety.The precipitable property of the PLS on salt additions,ligand immobilization,affinity adsorption kinetics and adsorption isotherms were studied.Trypsin was purified by affinity precipitation from a crude pancreatic extract.The specific activity reached about 13000 U.mg－1. These results indicate that the PLS is a potential ligand carrier for affinity precipitation of proteins.
