The aim of this paper is to report a new coding variance of the TNFRSF9 gene,a candidate for auto-immune diseases.We found the variation in two families with type 2 diabetes mellitus by D-HPLC mutation screening metho...The aim of this paper is to report a new coding variance of the TNFRSF9 gene,a candidate for auto-immune diseases.We found the variation in two families with type 2 diabetes mellitus by D-HPLC mutation screening method and confirmed our results by direct sequencing and PCR-RFLP.Although without changing the amino acid coding,the variance may have an effect on codon usage and play a role in disease development,such as type 2 diabetes mellitus.However,we cannot define the role of this variance because the frequency of the minor allele is low in the Chinese population and no homozygote of the variance was found.More research in multiple populations will be necessary to define the role of this variance.展开更多
Microbes are vital to the earth because of their enormous numbers and instinct function maintaining the natural balance. Since the microbiology was applied in environmental science and engineering more than a century ...Microbes are vital to the earth because of their enormous numbers and instinct function maintaining the natural balance. Since the microbiology was applied in environmental science and engineering more than a century ago, researchers desire for more and more information concerning the microbial spatio-temporal variations in almost every fields from contaminated soil to wastewater treatment plant （WWTP）. For the past 30 years, molecular biologic techniques explored for environ- mental microbial community （EMC） have spanned a broad range of approaches to facilitate the researches with the assistance of computer science： faster, more accurate and more sensitive. In this feature article, we outlined several current and emerging molecular biologic techniques applied in detection of EMC, and presented and assessed in detail the application of three promising tools.展开更多
基金supported by grants from the Major State Basic Research Development Program of China(973 Program)(No.2006CB503901)the National High Technology Research and Development Program of China(863 Program)(No.2002AA223031)The National Scientific Foundation of China(No.30471926,30671110).
文摘The aim of this paper is to report a new coding variance of the TNFRSF9 gene,a candidate for auto-immune diseases.We found the variation in two families with type 2 diabetes mellitus by D-HPLC mutation screening method and confirmed our results by direct sequencing and PCR-RFLP.Although without changing the amino acid coding,the variance may have an effect on codon usage and play a role in disease development,such as type 2 diabetes mellitus.However,we cannot define the role of this variance because the frequency of the minor allele is low in the Chinese population and no homozygote of the variance was found.More research in multiple populations will be necessary to define the role of this variance.
文摘Microbes are vital to the earth because of their enormous numbers and instinct function maintaining the natural balance. Since the microbiology was applied in environmental science and engineering more than a century ago, researchers desire for more and more information concerning the microbial spatio-temporal variations in almost every fields from contaminated soil to wastewater treatment plant （WWTP）. For the past 30 years, molecular biologic techniques explored for environ- mental microbial community （EMC） have spanned a broad range of approaches to facilitate the researches with the assistance of computer science： faster, more accurate and more sensitive. In this feature article, we outlined several current and emerging molecular biologic techniques applied in detection of EMC, and presented and assessed in detail the application of three promising tools.
