The HA is present in almost all vertebrates and plays a critical role in tissue development and cell proliferation, it has been demonstrated to promote wound healing and involved in angiogenesis and inflammation. Also...The HA is present in almost all vertebrates and plays a critical role in tissue development and cell proliferation, it has been demonstrated to promote wound healing and involved in angiogenesis and inflammation. Also polynucleotydes (PN) have proved to promote the “in vitro” growth and activity of human fibroblasts and osteoblasts, to increase reparation on UVB damaged dermal fibroblasts and seems to promote proliferation of human pre-adipocytes. Several in vivo studies have demonstrated the PN effect also in vivo, inducing an increase of angiogenesis and healing process. In this paper we have evaluated the effect of a mixture of Polynucleotides (PN) and entire Hyaluronic Acid (HA) on cultured human fibroblasts by analyzing cell growth. Different mixture have been tested and it has been demonstrated that the presence of HA even at low concentration (1 mg/ml) determine an increase of PN activity up to 20%. Furthermore, the addition of HA 1 mg/ml to PN 100 μg/ml induces a cell growth rate comparable to that exerted by PN concentration of 12 μg/ml.展开更多
The transient absorption spectra of 1-naphthylacetic acid in tetrahydrofuran have been obtained by laser flash photolysis technique. The transient species bands are at 509, 535 and 553 nm. These peak intensities incre...The transient absorption spectra of 1-naphthylacetic acid in tetrahydrofuran have been obtained by laser flash photolysis technique. The transient species bands are at 509, 535 and 553 nm. These peak intensities increase in the maximum values at 10.4 mu s.展开更多
A supramolecular inclusion complex between β-cyclodextrin（β-CD） and α-naphthylacetic acid was prepared, and its crystal structure was investigated by single-crystal X-ray crystallography. The complex contains two...A supramolecular inclusion complex between β-cyclodextrin（β-CD） and α-naphthylacetic acid was prepared, and its crystal structure was investigated by single-crystal X-ray crystallography. The complex contains two β-CD molecules, one α-naphthylacetic acid, two ethanols and twenty-eight water molecules in the asymmetric unit, which could be formulated as [（C42H70O35）2·（C12H10O2）·（C2H5OH）2·28H2O]. Two β-CD molecules constitute a dimer by face-to-face contact of their secondary hydroxyl sides. At the interface of the dimer, one α-naphthylacetic acid molecule is sandwiched between two β-CD molecules. Each β-CD unit of the dimer includes one ethanol molecule in its cavity. The β-CD dimers are linked together via hydrogen bonding to form layers that are stacked in a brickwork-like pattern. The comparative study of some sandwich complexes elucidates that the interface of the β-CD dimer has a stronger inclusion capacity than the cavity of β-CD for some suitable planar guest molecules. The novel inclusion structure results from the competitive inclusion of α-naphthylacetic acid and ethanol.展开更多
Poly(ADP-ribose) polymerase(PARP) is a protein-modifying and nucleotide-polymerizing enzyme. As a critical element in DNA repair, PARP can be activated by DNA strand breaks. Excessive activation of PARP, however, can ...Poly(ADP-ribose) polymerase(PARP) is a protein-modifying and nucleotide-polymerizing enzyme. As a critical element in DNA repair, PARP can be activated by DNA strand breaks. Excessive activation of PARP, however, can deplete NAD + and ATP, leads to cell death. Cleavage of PARP by activated caspase-3 play an important role in cell apoptosis.展开更多
以 CEA 为靶抗原的基因免疫治疗是近年来肿瘤治疗学研究的突出进展,我们用 CEA 真核表达质粒(pCEA)免疫兔子,分别以重组人 CEA 痘苗病毒(rV-CEA)和生理盐水作阳性和阴性对照。与生理盐水相比,pCEA 组和 rV-CEA 组兔血清 CEA 含量均明显...以 CEA 为靶抗原的基因免疫治疗是近年来肿瘤治疗学研究的突出进展,我们用 CEA 真核表达质粒(pCEA)免疫兔子,分别以重组人 CEA 痘苗病毒(rV-CEA)和生理盐水作阳性和阴性对照。与生理盐水相比,pCEA 组和 rV-CEA 组兔血清 CEA 含量均明显升高,并出现高滴度的作用时间持续更长久。展开更多
文摘The HA is present in almost all vertebrates and plays a critical role in tissue development and cell proliferation, it has been demonstrated to promote wound healing and involved in angiogenesis and inflammation. Also polynucleotydes (PN) have proved to promote the “in vitro” growth and activity of human fibroblasts and osteoblasts, to increase reparation on UVB damaged dermal fibroblasts and seems to promote proliferation of human pre-adipocytes. Several in vivo studies have demonstrated the PN effect also in vivo, inducing an increase of angiogenesis and healing process. In this paper we have evaluated the effect of a mixture of Polynucleotides (PN) and entire Hyaluronic Acid (HA) on cultured human fibroblasts by analyzing cell growth. Different mixture have been tested and it has been demonstrated that the presence of HA even at low concentration (1 mg/ml) determine an increase of PN activity up to 20%. Furthermore, the addition of HA 1 mg/ml to PN 100 μg/ml induces a cell growth rate comparable to that exerted by PN concentration of 12 μg/ml.
文摘The transient absorption spectra of 1-naphthylacetic acid in tetrahydrofuran have been obtained by laser flash photolysis technique. The transient species bands are at 509, 535 and 553 nm. These peak intensities increase in the maximum values at 10.4 mu s.
基金Supported by the Natural Science Foundation of Hainan Province, China(No.808145)the Doctoral Foundation of Hainan Normal University, China(No.103303)the Key Discipline of Hainan Province Organic Chemistry, China(No.006).
文摘A supramolecular inclusion complex between β-cyclodextrin（β-CD） and α-naphthylacetic acid was prepared, and its crystal structure was investigated by single-crystal X-ray crystallography. The complex contains two β-CD molecules, one α-naphthylacetic acid, two ethanols and twenty-eight water molecules in the asymmetric unit, which could be formulated as [（C42H70O35）2·（C12H10O2）·（C2H5OH）2·28H2O]. Two β-CD molecules constitute a dimer by face-to-face contact of their secondary hydroxyl sides. At the interface of the dimer, one α-naphthylacetic acid molecule is sandwiched between two β-CD molecules. Each β-CD unit of the dimer includes one ethanol molecule in its cavity. The β-CD dimers are linked together via hydrogen bonding to form layers that are stacked in a brickwork-like pattern. The comparative study of some sandwich complexes elucidates that the interface of the β-CD dimer has a stronger inclusion capacity than the cavity of β-CD for some suitable planar guest molecules. The novel inclusion structure results from the competitive inclusion of α-naphthylacetic acid and ethanol.
文摘Poly(ADP-ribose) polymerase(PARP) is a protein-modifying and nucleotide-polymerizing enzyme. As a critical element in DNA repair, PARP can be activated by DNA strand breaks. Excessive activation of PARP, however, can deplete NAD + and ATP, leads to cell death. Cleavage of PARP by activated caspase-3 play an important role in cell apoptosis.
文摘以 CEA 为靶抗原的基因免疫治疗是近年来肿瘤治疗学研究的突出进展,我们用 CEA 真核表达质粒(pCEA)免疫兔子,分别以重组人 CEA 痘苗病毒(rV-CEA)和生理盐水作阳性和阴性对照。与生理盐水相比,pCEA 组和 rV-CEA 组兔血清 CEA 含量均明显升高,并出现高滴度的作用时间持续更长久。
