This study was conducted to optimize the hydrolysis of porcine serum protein by response surface methodology （RSM）. Based on single-factor tests, 3- factor 3-level response surface tests were designed by Box-Behnken...This study was conducted to optimize the hydrolysis of porcine serum protein by response surface methodology （RSM）. Based on single-factor tests, 3- factor 3-level response surface tests were designed by Box-Behnken design method, and the effects of pH, temperature and hydrolysis duration on the response values were analyzed by RSM method. The optimal enzymolysis conditions were obtained as follows： pH value of 7.7, temperature at 46.3 ℃ and time of 7.4 h, under which the predicted degree of hydrolysis was 35.52%, and the chelating rate was 79.41%. This process could provide new ideas for the development of porcine serum protein.展开更多
To establish an animal model of acute-on-chronic liver failure(ACLF) that would replicate the pathological process of ACLF in humans, rats were administered porcine serum(PS) for 11 weeks. Liver fibrosis was determine...To establish an animal model of acute-on-chronic liver failure(ACLF) that would replicate the pathological process of ACLF in humans, rats were administered porcine serum(PS) for 11 weeks. Liver fibrosis was determined by pathological and biochemical assessments. The animals then were injected with D-galactosamine(D-gal) and lipopolysaccharide(LPS). The survival times of animals with cirrhosis and ACLF were determined over 48 h. Other animals were killed at 0, 4, 8 and 12 h after administration of D-gal/LPS. Liver injury was assessed by histopathological analysis and biochemical indices, and apoptosis was detected by Western blot and TUNEL analysis. After PS administration for 11 weeks the serum levels of hyaluronic acid and N-procollagen type III peptide increased significantly, and serious fibrosis and cirrhosis was observed at weeks 10 and 11. Cirrhotic rats were injected with D-gal/LPS to induced ACLF; the rate of mortality over 48 h was 80%. ALT and AST levels increased markedly at 4 h, but decreased significantly at8 and 12 h post-treatment. The total bilirubin, direct bilirubin, and total bile acids levels increased markedly at 8 and 12 h. Clotting times, TNF-α and IL-6 levels increased significantly, except for 12 h post-treatment.Apoptosis, inflammation and necrosis were elevated as determined by hematoxylin-eosin staining and TUNEL assays. BCL-2 levels decreased significantly, While BAX levels increased significantly.Cytochrome c expression peaked at 8 h postD-gal/LPS treatment. In conclusion, an ACLF model induced by PS and D-gal/LPS was established and the underlying mechanisms of ACLF development were explored.展开更多
文摘This study was conducted to optimize the hydrolysis of porcine serum protein by response surface methodology （RSM）. Based on single-factor tests, 3- factor 3-level response surface tests were designed by Box-Behnken design method, and the effects of pH, temperature and hydrolysis duration on the response values were analyzed by RSM method. The optimal enzymolysis conditions were obtained as follows： pH value of 7.7, temperature at 46.3 ℃ and time of 7.4 h, under which the predicted degree of hydrolysis was 35.52%, and the chelating rate was 79.41%. This process could provide new ideas for the development of porcine serum protein.
基金supported by 863 program(No.2014AA021101)from National High-tech R&D Program of Chinathe grants from National Sciences Foundation of China(Grant No.81573487)
文摘To establish an animal model of acute-on-chronic liver failure(ACLF) that would replicate the pathological process of ACLF in humans, rats were administered porcine serum(PS) for 11 weeks. Liver fibrosis was determined by pathological and biochemical assessments. The animals then were injected with D-galactosamine(D-gal) and lipopolysaccharide(LPS). The survival times of animals with cirrhosis and ACLF were determined over 48 h. Other animals were killed at 0, 4, 8 and 12 h after administration of D-gal/LPS. Liver injury was assessed by histopathological analysis and biochemical indices, and apoptosis was detected by Western blot and TUNEL analysis. After PS administration for 11 weeks the serum levels of hyaluronic acid and N-procollagen type III peptide increased significantly, and serious fibrosis and cirrhosis was observed at weeks 10 and 11. Cirrhotic rats were injected with D-gal/LPS to induced ACLF; the rate of mortality over 48 h was 80%. ALT and AST levels increased markedly at 4 h, but decreased significantly at8 and 12 h post-treatment. The total bilirubin, direct bilirubin, and total bile acids levels increased markedly at 8 and 12 h. Clotting times, TNF-α and IL-6 levels increased significantly, except for 12 h post-treatment.Apoptosis, inflammation and necrosis were elevated as determined by hematoxylin-eosin staining and TUNEL assays. BCL-2 levels decreased significantly, While BAX levels increased significantly.Cytochrome c expression peaked at 8 h postD-gal/LPS treatment. In conclusion, an ACLF model induced by PS and D-gal/LPS was established and the underlying mechanisms of ACLF development were explored.
