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Structure elucidation and in vitro rat intestinal fermentation properties of a novel sulfated glucogalactan from Porphyra haitanensis 被引量:3
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作者 Peilin Chen Lu Liu +4 位作者 Zirun Cheng Yi Zhang Baodong Zheng Xiaoke Hu Hongliang Zeng 《Food Science and Human Wellness》 SCIE CSCD 2023年第2期596-606,共11页
This study was to investigate the structure and rat fecal microbial fermentation properties of a polysaccharide fraction(PHP2)isolated from the red marine alga Porphyra haitanensis.PHP2 was characterized as a sulfated... This study was to investigate the structure and rat fecal microbial fermentation properties of a polysaccharide fraction(PHP2)isolated from the red marine alga Porphyra haitanensis.PHP2 was characterized as a sulfated glucogalactan,with a hypothetical backbone structure of→4)Gα(1→6)G4 Sβ(1→4)Glc(1→and a side chain of Man(1→6)Glc.PHP2 had an irregular spherical chain conformation.The 16 S r RNA sequence analysis revealed that PHP2 modulated the rat fecal micro-flora composition,with a similar effect to inulin,changing the dominant genus(Lactobacillus and Escherichia-Shigella)and promoting the growth of organisms that degrade sulfur-containing polysaccharides,such as Desulfovibrio,Ruminococcaceae_UCG-005,and Ruminococcus_2.PHP2 can promote production of acetic,propionic and butyric acid by rat fecal micro-flora.Prediction of metabolic function suggested that PHP2 could modulate cholesterol metabolism.The sulfated glucogalactan fermentation behavior may be associated with its monosaccharide composition,chain branching and chain conformation.PHP2 appeared to have considerable potential as functional food,and was associated with sulfur-containing polysaccharides in general. 展开更多
关键词 porphyra haitanensis Structure characterization Fecal microflora Short-chain fatty acids Metabolic function prediction POLYSACCHARIDE
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Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis 被引量:2
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作者 李艳燕 沈颂东 +2 位作者 贺丽虹 许璞 王广策 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2009年第3期493-501,共9页
The sequences of the ITS(internal transcribed spacer) and 5.8S rDNA of three cultivated strains of Porphyra haitanensis thalli(NB,PT and ST) were amplified,sequenced and analyzed.In addition,the phylogenic relationshi... The sequences of the ITS(internal transcribed spacer) and 5.8S rDNA of three cultivated strains of Porphyra haitanensis thalli(NB,PT and ST) were amplified,sequenced and analyzed.In addition,the phylogenic relationships of the sequences identified in this study with those of other Porphyra retrieved from GenBank were evaluated.The results are as follows:the sequences of the ITS and 5.8S rDNA were essentially identical among the three strains.The sequences of ITS1 were 331 bp to 334 bp,while those of the 5.8S rDNA were 158 bp and the sequences of ITS2 ranged from 673 bp to 681 bp.The sequences of the ITS had a high level of homology(up to 99.5%) with that of P.haitanensis(DQ662228) retrieved from GenBank,but were only approximately 50% homologous with those of other species of Porphyra.The results obtained when a phylogenetic tree was constructed coincided with the results of the homology analysis.These results suggest that the three cultivated strains of P.haitanensis evolved conservatively and that the ITS showed evolutionary consistency.However,the sequences of the ITS and 5.8S rDNA of different Porphyra species showed great variations.Therefore,the relationship of Porphyra interspecies phyletic evolution could be judged,which provides the proof for Porphyra identification study.However,proper classifications of the subspecies and the populations of Porphyra should be determined through the use of other molecular techniques to determine the genetic variability and rational phylogenetic relationships. 展开更多
关键词 porphyra haitanensis ITS region sequence analysis INTERSPECIES PHYLOGENY
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In vitro Antioxidant Effects of Porphyra haitanensis Peptides on H_2O_2-Induced Damage in HepG2 Cells 被引量:1
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作者 CHEN Shengjun YU Jiao +4 位作者 HU Xiao YANG Xianqing LI Laihao QI Bo DENG Jianchao 《Journal of Ocean University of China》 SCIE CAS CSCD 2021年第2期421-428,共8页
In this study,protein from Porphyra haitanensis was used as raw material to prepare an antioxidant peptide,and its antioxidant activity was evaluated in vitro.A model of H_2O_2-induced oxidative damage in Hep G2 cells... In this study,protein from Porphyra haitanensis was used as raw material to prepare an antioxidant peptide,and its antioxidant activity was evaluated in vitro.A model of H_2O_2-induced oxidative damage in Hep G2 cells was established,and the effects of Porphyra haitanensis hydrolysates (PHHs) on superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were detected.Finally,the structure of PHHs was identified by ESI-MS/MS.The results showed that the 1,1-diphenyl-2-pyridylhydrazine(DPPH)-free radical-scavenging ability of PHHs was the strongest (59.28%at 1.0 mg m L~(-1)) when hydrolyzed with an acidic protease for 4 h.PHHs with different concentrations had protective effects on H_2O_2-induced damage to Hep G2 cells,and the protective effect was enhanced with increasing concentrations.When the level was 400μg m L~(-1),the cell survival rate was as high as 88.62%.Moreover,PHHs can significantly reduce oxidative damage to Hep G2 cells by H_2O_2,improve SOD activity,and reduce MDA content.The tetrapeptide Asp-Lys-Ser-Thr,with a molecular weight of 448 Da,was identified as an important fraction of PHHs by high-resolution mass spectrometry. 展开更多
关键词 porphyra haitanensis hydrolysates(PHHs) antioxidant peptides radical-scavenging activity cell antioxidant capacity electrospray ionization-mass spectrometry
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The UV-absorbing compounds in gametophytic blades of Porphyra haitanensis and the effect on formation of sporangial branchlets of conchocelis
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作者 陈烨 严小军 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2007年第3期343-346,共4页
Endogenous levels of UVAC in different parts of matured gametophytic blades of Porphyra haitanensis and the effect of exogenous UVAC on sporangial branchlet formation are studied. The UVAC value in vegetative (36. 78 ... Endogenous levels of UVAC in different parts of matured gametophytic blades of Porphyra haitanensis and the effect of exogenous UVAC on sporangial branchlet formation are studied. The UVAC value in vegetative (36. 78 mg/g) and reproductive (29.25 mg/g) parts of female thallus is higher than their counterparts in male thallus (34.68 mg/g and 20.46 mg/g). The UVAC value of reproductive parts is higher than vegetative parts of same sexual thallus. The optimal UVAC concentration for sporangial branchlet formation is 0.1 mg/g. 展开更多
关键词 UV-absorbing compounds porphyra haitanensis REPRODUCTION sporangial branchlets
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Porphyra haitanensis polysaccharide(PH)attenuates cell hyperplasia via remodeling the cross-talk between Hippo/YAP and mTOR pathways
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作者 Chong Wang Wanglei Lin +3 位作者 Zhihua Sun Yiming Sun Yanbo Wang Linglin Fu 《Food Science and Human Wellness》 SCIE CSCD 2023年第2期424-430,共7页
Porphyra polysaccharide is a special kind of nutrient showing multiple physiological functions including regulating cell proliferation,but the detailed mechanisms are not fully revealed,impairing its further developme... Porphyra polysaccharide is a special kind of nutrient showing multiple physiological functions including regulating cell proliferation,but the detailed mechanisms are not fully revealed,impairing its further development and applications.This work was to purify and characterize the Porphyra haitanensis polysaccharide(PH),investigate its physiological function,and demonstrate the underlying mechanisms.The purified PH was first characterized by Fourier-transform infrared spectroscopy.Then an intestinal epithelial cell model was established,in which PH significantly suppressed cell hyperplasia.Specifically,PH activated the Hippo/YAP pathway,which subsequently activated m TOR pathway,however m TOR activated Hippo in the absence of PH.Moreover,both the inhibition of Hippo by YAP1 knock-down and the suppression of m TOR by rapamycin impaired PH function.These results indicated that PH attenuated hyperplasia activity by remodeling the cross-talk between Hippo/YAP and m TOR pathways,which revealed potential targets and approaches for treating hyperplasia-related diseases and provided novel ways to utilize P.haitanensis as well as other related functional foods. 展开更多
关键词 porphyra haitanensis POLYSACCHARIDE HIPPO MTOR Cancer
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Subunit composition and chromophore content of R-phycocyanin and allophycocyanin from Porphyra haitanensis
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作者 Gao Hongfeng Cao Wenda and Ji Minghou 《Acta Oceanologica Sinica》 SCIE CAS CSCD 1994年第2期253-260,共8页
R-phycocyanin (RPC) of Porphyra haitanensis (T. J. Chang et B. F. Zheng )' was chromatographed on Bio-Rex 70 column with urea solution (pH 3. 0) as an eluent, and a and β two subunits were isolated.Their molecula... R-phycocyanin (RPC) of Porphyra haitanensis (T. J. Chang et B. F. Zheng )' was chromatographed on Bio-Rex 70 column with urea solution (pH 3. 0) as an eluent, and a and β two subunits were isolated.Their molecular weights were determined on SDS-PAGE at 18 400 and 20 500, respetively,while those of a and β subunits of allophycocyanin (APC) at 18 800 and 19 700, respectively,and those of RPC and APC were at 117 000 and 122 000,respectively.Both the molar ratio of a and β subests of RPC and APC were 1:1, and the subunit composition was confirmed to be (αβ )3.It was ascertained that in RPC αsubunit contains one chromophore phycocyanobilin (PCB) and β subunit has one chromophore PCB and one phycoerythrobilin (PEB), while in APC both α and βsubunits contain one PCB. 展开更多
关键词 porphyra haitanensis R-phycocyanin ALLOPHYCOCYANIN subunit and chromophore.
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