Portable and automated analyzer for rapid and high precision in vitro dissolution of drugs

We developed a novel portable and automated dissolution test analyzer for rapid and high precision in vitro dissolution testing of drugs.The analyzer consists of a flow-through-cell drug dissolution system,an automated sequential sampling system,a high-speed capillary electrophoresis(HSCE)system,and a data acquisition system.Combining the high-temporal resolution flow-gating sampling approach with HSCE,which has outstanding advantages of efficient separation and resolution,the analyzer can achieve rapid analysis and exhibits the ability in miniaturization for on-site assessment of different active pharmaceutical ingredients.To integrate the flow-through-cell dissolution system with HSCE,a specially designed flow-gating-injection(FGI)interface was employed.The performance of the analyzer was investigated by analyzing the dissolution of immediate-release drugs including single dose(amoxicillin dispersible tablets)and fixed dose combination(amoxicillin and clavulanate potassium)drug tablets with the high-temporal resolutions of 12 s and 20 s,respectively.The dissolution profiles of different active pharmaceutical ingredients could be simultaneously and automatically monitored with high repeatability and accuracy.The analyzer was successfully utilized for the pharmaceutical quality control and bio-relevant dissolution testing,as well as in vivo-in vitro correlation analysis.Our portable analyzer is miniaturized,convenient and of low-cost,and will provide a valuable tool for dissolution testing in pharmaceutical research and development.

Condition Assessment of August A. Busch Bridge Deck Using Portable Seismic Property Analyzer and Ground Penetrating Radar

Ground penetrating radar (GPR) and the portable seismic property analyzer (PSPA) have been extensively used in the past two decades for monitoring, quantifying, and mapping the deterioration of bridge decks. Using PSPA and GPR ensures regular monitoring of bridge conditions, leads to the early detection of deterioration. This research is to address the condition of August A. Busch bridge deck owned by the Missouri Department of Conservation. Visual inspection, GPR, and PSPA data were acquired on the bridge deck. Over 90% of the bridge deck was in fair to good condition with an average compressive strength of over 2500 psi. GPR data showed no indication of significant deterioration. The overall bridge deck was determined to be in fair to good condition.

Application of Dynamic Linear Detecting Method in Data Processing of the Portable Blood Sugar Analyzer

In this paper,a dynamic linear detecting method,that the non-linear coefficient NL% was led and the non-linearity of data were estimated continuously and dynamically and determined when NL% exceeded reference value (5%),was used for data processing and could solve the problem caused by the phenomenon of substrate depleting occurred following the redox reaction in portable blood sugar analyzer.By contrast to the conventional end-point method,the dynamic linear detecting method is based on multipoint data collecting.Experiments of measuring the calibration glucose solution with 8 various concentrations from 50 mg/dl to 400 mg/dl were carried out with the analyzer developed by our group.The linear regression curve,whose correlation for the data was 0.9995 and the residual was 2.8080,were obtained.The obtained correlation,residual, and the computation workload are all fit for the portable blood sugar analyzer.

Low-cost portable dielectric spectrometer based on mini-vector network analyzer and open-ended coaxial probe technology

As a simple,fast,and non-destructive measuring technology,dielectric spectroscopy is usually used to analyze the dielectric properties of agricultural products and food,and then to predict the main components of materials.However,the large and expensive vector network analyzers(VNA)with expensive analysis software applied in measuring dielectric properties make research limited to the laboratory.To acquire dielectric spectra in situ,a model for solving relative complex permittivity was derived,and its performance was validated.Then,a low-cost portable dielectric spectrometer with a mini VNA,a Raspberry Pi,and a coaxial probe as core parts was developed over the frequency range of 100-3000 MHz.The stability and accuracy of the developed spectrometer were tested using milk and juice.The results indicated that the relative errors of the model were within±5%for dielectric constant(ε′)and loss factor(ε″).The coefficients of variation of measuredε′andε″by the developed spectrometer at 100-3000 MHz were less than 1%and 2%,respectively.Compared with the dielectric properties obtained by using a commercial dielectric measurement system,the relative errors of measuredε′andε″were within±3.4%and±6.0%,respectively.This study makes fast,non-destructive,and on-site food quality detection using dielectric spectra possible.

便携式Li-K分析仪的研制及其在锂辉石中锂的分析应用

我国锂钾主要矿产资源大多分布在西部偏远地区,其勘查找矿或综合利用迫切需要现场快速分析技术的支持。本文介绍了自行开发的便携式Li-K分析仪的主要性能及其在锂辉石中锂的分析应用。Li-K分析仪是一种基于大气压的液体阴极辉光放电光谱仪,以待测液体为放电阴极,实现了样品中Li、K等元素的原子化和激发。该仪器以光纤传导CCD光谱仪作为检测器,其波长范围为345-1015 nm,分辨率3 nm;以Li 670.78 nm和K 769.90 nm分析谱线,在仪器最佳工作条件下测定Li、K的精密度（RSD,n=14）均低于2%,检出限为0.03μg/m L,检测范围0.1-10μg/m L。研究表明,不同酸度和酸的类型对谱线强度影响较大,且存在显著的样品基体效应,标准曲线法分析锂辉石中Li的结果偏差高达267%;而标准加入法可克服基体效应的影响,获得与ICP-OES一致性较好的分析结果,为现场开展固体样品中Li的测定奠定了基础。

Portable Analyzer for Rapid Analysis of Total Protein, Fat and Lactose Contents in Raw Milk Measured by Non-dispersive Short-wave Near-infrared Spectrometry

A novel portable analyzer for raw milk quality control during the material purchase at dairy plants was developed, by which the percentages（mass fraction） of main components including total protein, fat, and lactose of an unhomogenized milk sample could be determinated in 1 min with the help of non-dispersive short-wave near-infrared （NDSWNIR） spectrometry in a wavelength range from 600 nm to 1100 nm and multivariate calibration. The analyzer was designed with a single-beam optical system, which comprised a temperature control module, a multi-channel narrow-band light source（16 wavelengths）, a glass absorption cell with 15 mm sample thickness, a silicon photodiode detector, several compound lenses and a recorder module. A total of 80 raw milk samples were collected at a dairy farm twice a month for 4 months. The samples were scanned with a common UV-Vis-NIR spectrometer and analyzed according to China GB standard methods. The uninformative variables elimination（UVE） method was carried out on the spectrum data and the percentages of main components of all the samples to choose the peak emitting wavelength of each channel of the light source. Another 90 raw milk samples were collected from the same dairy farm thrice a month for 3 months. The samples were analyzed according to China GB standard methods and with the proposed analyzer. The percentages of the main components and the NDSWNIR absorption data of the samples were used for the construction and validation of the multivariate calibration model with partial least squares（PLS） method. The root-mean-square errors of prediction（RMSEP） of total protein, fat and lactose were 0.201, 0.172 and 0.247 and the coefficients of correlation（R） were 0.932, 0.981 and 0.933, respectively.

Multiplexed detection of respiratory pathogens with a portable analyzer in a “raw-sample-in and answer-out” manner

Coronavirus disease 2019(COVID-19)has emerged,rapidly spread and caused significant morbidity and mortality worldwide.There is an urgent public health need for rapid,sensitive,specific,and on-site diagnostic tests for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.In this study,a fully integrated and portable analyzer was developed to detect SARS-CoV-2 from swab samples based on solid-phase nucleic acid extraction and reverse transcription loop-mediated isothermal amplification(RT-LAMP).The swab can be directly inserted into a cassette for multiplexed detection of respiratory pathogens without pre-preparation.The overall detection process,including swab rinsing,magnetic bead-based nucleic acid extraction,and 8-plex real-time RT-LAMP,can be automatically performed in the cassette within 80 min.The functionality of the cassette was validated by detecting the presence of a SARS-CoV2 pseudovirus and three other respiratory pathogens,i.e.,Klebsiella pneumoniae,Pseudomonas aeruginosa,and Stenotrophomonas maltophilia.The limit of detection(LoD)for the SARS-CoV-2 pseudovirus was 2.5 copies/μL with both primer sets(N gene and ORF1ab gene),and the three bacterial species were successfully detected with an LoD of 2.5 colony-forming units(CFU)/μL in 800μL of swab rinse.Thus,the analyzer developed in this study has the potential to rapidly detect SARS-CoV-2 and other respiratory pathogens on site in a“raw-sample-in and answer-out”manner.

便携式数字多道脉冲幅度分析器的设计

为便于野外测试,设计了一款便携式的数字多道脉冲幅度分析器。采用ARM+FPGA的设计方案,通过嵌入式Linux系统实现控制和用户交互。脉冲信号经调理电路处理后,由采样电路转为数字信号,并在FPGA中完成滤波成形等数字处理,最终由嵌入式系统处理并显示能谱图。采用银粉样品测试,全能峰处能量分辨率为2.80%,道址漂移在2个道址以内,能够满足设计要求。

便携式锂钾分析仪现场测定热泉水中的锂

锂是绿色能源和轻质合金的理想原料,作为一种重要的战略性资源而备受各国重视。热泉水中富含锂,储量可观,然而热泉水主要分布在西藏、云南等偏远地区,样品运输与实验室测试成本高、效率低,锂资源勘查急需现场分析技术的支持。热泉水一般含有较高浓度的钠、钾等元素,基体效应显著。本文利用自主研发的便携式锂钾分析仪,搭配负性滤光片,选择锂的分析谱线波长670.78nm,通过优化测量条件,建立了标准曲线法与标准加入法现场测定热泉水中锂元素的分析方法。实验结果表明,当电解质是体积分数为1.5%的盐酸,工作电流为70mA,进样流速为3.0mL/min时,锂检出限为4.07μg/L,相对标准偏差(RSD)为1.03%。对热泉水样品进行加标测试,加标回收率为81.6%~115.9%。当热泉水样品基体组成较简单时,直接采用标准曲线法即可获得较准确的分析结果;当样品基体组成较复杂或者基体浓度高时,采用标准加入法可有效地减小基体效应,获得的分析结果相对更准确。本方法适用于不同类型基体的热泉水中锂含量的分析测试。

阳极溶出伏安法便携式重金属分析仪性能测试方法研究

阳极溶出伏安法便携式重金属分析仪被广泛应用于水体、土壤、食品、固态物质的重金属含量检测。除镉、铅、铜外,使用该分析仪检测砷、汞、锌、铬、锰、镍、锡、铊、铁、钴、硒、银、碲、金、铋等重金属时,需选择合适的计量性能测试项目及测试方法。同时,JJF 2037-2023《便携式溶出伏安法重金属检测仪校准规范》对测量镉、铅、铜元素的便携式溶出伏安法重金属检测仪的计量特性及校准方法做出了要求。

医用便携式血糖仪与生化分析仪检测结果的对比研究

目的评价医用便携式血糖仪在临床血糖检测方面的性能,为医疗机构采购便携式血糖仪提供参考。方法本研究采集2024年3月10日温州医科大学附属第一医院的5份人体静脉全血样本,血糖浓度为1.7~3.5mmol/L、3.5~6.1mmol/L、6.2~8.3mmol/L、8.4~13.9mmol/L、14.0~25.0mmol/L。分别采用6个品牌各10台血糖仪和生化分析仪重复检测2次,参照《便携式血糖仪临床操作和质量管理指南WS/T781-2021》评价各品牌血糖仪的准确度、精密度及与生化分析仪的相关性和可比性。结果当血糖浓度<5.5 mmol/L时,6个品牌血糖仪绝对偏差分布在-0.52~+0.54 mmol/L,标准差(s)分布在0.04~0.13mmol/L;当血糖浓度≥5.5mmol/L时,相对偏差分布在-19.88%~12.78%,变异系数(CV)分布在1.3%~3.65%。6个品牌血糖仪与生化分析仪相关系数分布在0.997~0.999,检测结果偏差范围的符合率分布在91%~100%,其中有5个品牌符合率>95%,1个品牌相对偏差超出±15%。结论6个品牌便携式血糖仪精密度均较好,与生化分析仪检测结果有较好相关性。5个品牌便携式血糖仪准确度较好,1个品牌准确度未达到指南要求。医疗机构采用新型号的血糖仪前,有必要进行精密度、准确度和相关性等验证。

便携式重金属分析仪测定水中14种重金属元素

近年来,重金属污染突发事故时有发生,危害人民的财产和人身安全。为突发事故风险评估和补救措施制定提供及时可靠的数据支持,发展一种快速、便捷、准确的现场应急监测方法迫在眉睫。为满足工作需求,本文采用便携式重金属分析仪对水中Cu、Cd、Pb、Zn、Hg、As、TI、Mn、Ag、Sb、Ni、Cr、Fe和Co14种元素进行测定,溶出伏安法监测中Cu、Cd、Pb、Zn、Hg、As、TI、Mn、Ag、Sb和Ni的检出限为0.14~1.47μg/L,比色法中Cu、Cr、Ni、Pb、Fe、Co和Mn元素的检出限为4.8~23.5μg/L,样品加标回收率为84.0%~117%,平行测定3次的RSD<5%。

XRF和PXRF测定土壤中10种重金属元素方法研究

本文采用台式X射线荧光光谱仪(台式XRF)和便携式X射线荧光分析仪(PXRF)分别对土壤中Cu、Cr、Pb、Zn、Ni、As、Co、V、Mn和Ti等10种元素进行监测分析。采用台式XRF分析这10种元素在各自相应的范围内线性良好,r=0.9990~0.9999,检出限为0.8 mg/kg~5.5 mg/kg,平行测定3次的RSD<2%;而采用PXRF测定,RSD<10%,准确度明显低于台式XRF,对土壤中Cr、Zn、Ni、As、Co、V和Mn等部分元素仅可进行半定量分析。X射线荧光光谱法简化了土壤样品分析,解决了传统的检测分析方法,耗时长,前处理复杂,强酸的使用易导致环境的二次污染等问题,在土壤重金属分析中具有明显的优势。

便携式车辆平稳性测量分析仪在动车组上的应用建议

随着智能化程度和对动车组的监控要求提高,复兴号动车组通过车载装置即可对车辆运行平稳性进行实时监控,本文基于中国和谐号动车组平稳性检测现状与实际需求,介绍了便携式车辆平稳性测量分析仪的使用情况,通过对便携式仪器与复兴号动车组车载平稳性装置测量数据的对比分析,结合便携式仪器使用经验,以减少对乘客的影响的同时提高便携式仪器所测数据的准确性为目的,最终提出了使用便携式车辆平稳性测量分析仪检测和谐号动车组平稳性的建议方案。

便携式列车工业以太网性能分析仪研制

文章中设计了一种便携式列车工业以太网性能分析仪,用于动车组和城轨车辆多场景下的工业以太网性能测试和智能分析。该分析仪具备人机交互界面、独立电池、丰富的接口、可扩展内存、高频数据采集器和专业测试软件。分析仪可实时监控分析以太网数据,并检查MAC、IP、带宽、TRDP报文、数据包等参数是否满足技术要求。同时,该分析仪还具有自动化预警功能和长时间记录网络数据的功能,可用于定位和解决列车运行中偶发且难以捕捉的网络故障。目前,该分析仪已应用于时速250 km中国标准动车组项目和深圳地铁20号线等项目中,实现了快速全面地对列车工业以太网网络进行测试和诊断。

余氯、总氯现场快速测定仪的研制和应用

研制了一种基于光度法现场测定水中余氯、总氯的手持式仪器 ,开发了相应的专用试剂包 ,讨论了影响该仪器分析性能的主要因素。用该仪器和试剂现场测定水中的余氯和总氯 ,线性范围是 0 .0 1～ 5 .0mg/L ;RSD <3% ;单个样品分析时间不超过 3min。

地震后危重多发伤患者野外条件下的救治体会

目的:探讨突发灾难后危重多发伤患者在野外条件下的救治方案。方法和结果:患者女性,53岁,因"地震中骨盆砸伤、大量血尿"入院,呼吸困难,全腹压痛,褥疮。便携式B超示腹腔积液,左胸腔积液,膀胱破裂。便携式血气分析仪提示"严重贫血+低氧血症+低钾+酸中毒"。术前行左胸腔穿刺,膀胱穿刺引流。剖腹探查见直肠破裂(穿孔),遂行Harttman手术加膀胱造瘘术和抗感染治疗。术后第18日基本恢复。结论:术者具有精湛的技术和丰富的经验、充分发挥便携式设备的作用、依靠具有地理优势的当地医院、积极抗感染是艰苦环境下救治危重多发伤患者的成功因素。

新型高灵敏度XRF分析仪的研制与应用

新型高灵敏度便携式X射线荧光(XRF)分析仪基于电致冷Si-PIN半导体X射线探测器和微机多道(2048道)谱仪系统,整机重3.8kg(探头重0.6kg),对铜、锌、砷等元素的分析检出限为10μg·g-1,适用于野外现场的天然岩石、土壤和水系沉积物的多元素含量的定量测定,以及工业原料、半成品、产品快速分析。

新一代高灵敏度手提式X荧光仪的研制

介绍了新一代高灵敏度手提式X射线荧光仪 ,该仪器由电制冷Si PIN半导体X射线探测器、放射性同位素X射线激发源和基于PC/ 10 4工控机的 4 0 96道多道脉冲幅度分析器组成 ,对铜、锌、砷等元素的分析检出限为 10× 10 -6,适用于野外现场的天然岩石、土壤和水系沉积物的多元素含量的定量测定 ,基本满足新一轮地质大调查的需要 ,特别是二、三级异常点 (带 )的快速查证与野外追索工作。

铁矿石XRF现场取样技术的研究与应用

介绍铁矿石Ｘ射线荧光分析（ＸＲＦ）现场取样的技术及其在武钢金山店铁矿的应用效果。主要讨论ＸＲＦ现场取样中的几何效应、基体效应、水份及矿化不均匀性的影响和校正办法．试验表明，ＸＲＦ现场取样的合格率达到８７．６％，超过了传统的人工取样化验方法的重现性，其速度比传统方法至少提高２００倍，并明显地降低了生产成本，提高了经济效益．