Portable and automated analyzer for rapid and high precision in vitro dissolution of drugs

We developed a novel portable and automated dissolution test analyzer for rapid and high precision in vitro dissolution testing of drugs.The analyzer consists of a flow-through-cell drug dissolution system,an automated sequential sampling system,a high-speed capillary electrophoresis(HSCE)system,and a data acquisition system.Combining the high-temporal resolution flow-gating sampling approach with HSCE,which has outstanding advantages of efficient separation and resolution,the analyzer can achieve rapid analysis and exhibits the ability in miniaturization for on-site assessment of different active pharmaceutical ingredients.To integrate the flow-through-cell dissolution system with HSCE,a specially designed flow-gating-injection(FGI)interface was employed.The performance of the analyzer was investigated by analyzing the dissolution of immediate-release drugs including single dose(amoxicillin dispersible tablets)and fixed dose combination(amoxicillin and clavulanate potassium)drug tablets with the high-temporal resolutions of 12 s and 20 s,respectively.The dissolution profiles of different active pharmaceutical ingredients could be simultaneously and automatically monitored with high repeatability and accuracy.The analyzer was successfully utilized for the pharmaceutical quality control and bio-relevant dissolution testing,as well as in vivo-in vitro correlation analysis.Our portable analyzer is miniaturized,convenient and of low-cost,and will provide a valuable tool for dissolution testing in pharmaceutical research and development.

Condition Assessment of August A. Busch Bridge Deck Using Portable Seismic Property Analyzer and Ground Penetrating Radar

Ground penetrating radar (GPR) and the portable seismic property analyzer (PSPA) have been extensively used in the past two decades for monitoring, quantifying, and mapping the deterioration of bridge decks. Using PSPA and GPR ensures regular monitoring of bridge conditions, leads to the early detection of deterioration. This research is to address the condition of August A. Busch bridge deck owned by the Missouri Department of Conservation. Visual inspection, GPR, and PSPA data were acquired on the bridge deck. Over 90% of the bridge deck was in fair to good condition with an average compressive strength of over 2500 psi. GPR data showed no indication of significant deterioration. The overall bridge deck was determined to be in fair to good condition.

Application of Dynamic Linear Detecting Method in Data Processing of the Portable Blood Sugar Analyzer

In this paper,a dynamic linear detecting method,that the non-linear coefficient NL% was led and the non-linearity of data were estimated continuously and dynamically and determined when NL% exceeded reference value (5%),was used for data processing and could solve the problem caused by the phenomenon of substrate depleting occurred following the redox reaction in portable blood sugar analyzer.By contrast to the conventional end-point method,the dynamic linear detecting method is based on multipoint data collecting.Experiments of measuring the calibration glucose solution with 8 various concentrations from 50 mg/dl to 400 mg/dl were carried out with the analyzer developed by our group.The linear regression curve,whose correlation for the data was 0.9995 and the residual was 2.8080,were obtained.The obtained correlation,residual, and the computation workload are all fit for the portable blood sugar analyzer.

便携式锂钾分析仪现场测定热泉水中的锂

锂是绿色能源和轻质合金的理想原料,作为一种重要的战略性资源而备受各国重视。热泉水中富含锂,储量可观,然而热泉水主要分布在西藏、云南等偏远地区,样品运输与实验室测试成本高、效率低,锂资源勘查急需现场分析技术的支持。热泉水一般含有较高浓度的钠、钾等元素,基体效应显著。本文利用自主研发的便携式锂钾分析仪,搭配负性滤光片,选择锂的分析谱线波长670.78nm,通过优化测量条件,建立了标准曲线法与标准加入法现场测定热泉水中锂元素的分析方法。实验结果表明,当电解质是体积分数为1.5%的盐酸,工作电流为70mA,进样流速为3.0mL/min时,锂检出限为4.07μg/L,相对标准偏差(RSD)为1.03%。对热泉水样品进行加标测试,加标回收率为81.6%~115.9%。当热泉水样品基体组成较简单时,直接采用标准曲线法即可获得较准确的分析结果;当样品基体组成较复杂或者基体浓度高时,采用标准加入法可有效地减小基体效应,获得的分析结果相对更准确。本方法适用于不同类型基体的热泉水中锂含量的分析测试。

阳极溶出伏安法便携式重金属分析仪性能测试方法研究

阳极溶出伏安法便携式重金属分析仪被广泛应用于水体、土壤、食品、固态物质的重金属含量检测。除镉、铅、铜外,使用该分析仪检测砷、汞、锌、铬、锰、镍、锡、铊、铁、钴、硒、银、碲、金、铋等重金属时,需选择合适的计量性能测试项目及测试方法。同时,JJF 2037-2023《便携式溶出伏安法重金属检测仪校准规范》对测量镉、铅、铜元素的便携式溶出伏安法重金属检测仪的计量特性及校准方法做出了要求。

便携式车辆平稳性测量分析仪在动车组上的应用建议

随着智能化程度和对动车组的监控要求提高,复兴号动车组通过车载装置即可对车辆运行平稳性进行实时监控,本文基于中国和谐号动车组平稳性检测现状与实际需求,介绍了便携式车辆平稳性测量分析仪的使用情况,通过对便携式仪器与复兴号动车组车载平稳性装置测量数据的对比分析,结合便携式仪器使用经验,以减少对乘客的影响的同时提高便携式仪器所测数据的准确性为目的,最终提出了使用便携式车辆平稳性测量分析仪检测和谐号动车组平稳性的建议方案。

Portable Analyzer for Rapid Analysis of Total Protein, Fat and Lactose Contents in Raw Milk Measured by Non-dispersive Short-wave Near-infrared Spectrometry

A novel portable analyzer for raw milk quality control during the material purchase at dairy plants was developed, by which the percentages（mass fraction） of main components including total protein, fat, and lactose of an unhomogenized milk sample could be determinated in 1 min with the help of non-dispersive short-wave near-infrared （NDSWNIR） spectrometry in a wavelength range from 600 nm to 1100 nm and multivariate calibration. The analyzer was designed with a single-beam optical system, which comprised a temperature control module, a multi-channel narrow-band light source（16 wavelengths）, a glass absorption cell with 15 mm sample thickness, a silicon photodiode detector, several compound lenses and a recorder module. A total of 80 raw milk samples were collected at a dairy farm twice a month for 4 months. The samples were scanned with a common UV-Vis-NIR spectrometer and analyzed according to China GB standard methods. The uninformative variables elimination（UVE） method was carried out on the spectrum data and the percentages of main components of all the samples to choose the peak emitting wavelength of each channel of the light source. Another 90 raw milk samples were collected from the same dairy farm thrice a month for 3 months. The samples were analyzed according to China GB standard methods and with the proposed analyzer. The percentages of the main components and the NDSWNIR absorption data of the samples were used for the construction and validation of the multivariate calibration model with partial least squares（PLS） method. The root-mean-square errors of prediction（RMSEP） of total protein, fat and lactose were 0.201, 0.172 and 0.247 and the coefficients of correlation（R） were 0.932, 0.981 and 0.933, respectively.

Multiplexed detection of respiratory pathogens with a portable analyzer in a “raw-sample-in and answer-out” manner

Coronavirus disease 2019(COVID-19)has emerged,rapidly spread and caused significant morbidity and mortality worldwide.There is an urgent public health need for rapid,sensitive,specific,and on-site diagnostic tests for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.In this study,a fully integrated and portable analyzer was developed to detect SARS-CoV-2 from swab samples based on solid-phase nucleic acid extraction and reverse transcription loop-mediated isothermal amplification(RT-LAMP).The swab can be directly inserted into a cassette for multiplexed detection of respiratory pathogens without pre-preparation.The overall detection process,including swab rinsing,magnetic bead-based nucleic acid extraction,and 8-plex real-time RT-LAMP,can be automatically performed in the cassette within 80 min.The functionality of the cassette was validated by detecting the presence of a SARS-CoV2 pseudovirus and three other respiratory pathogens,i.e.,Klebsiella pneumoniae,Pseudomonas aeruginosa,and Stenotrophomonas maltophilia.The limit of detection(LoD)for the SARS-CoV-2 pseudovirus was 2.5 copies/μL with both primer sets(N gene and ORF1ab gene),and the three bacterial species were successfully detected with an LoD of 2.5 colony-forming units(CFU)/μL in 800μL of swab rinse.Thus,the analyzer developed in this study has the potential to rapidly detect SARS-CoV-2 and other respiratory pathogens on site in a“raw-sample-in and answer-out”manner.

抗凝剂物质对便携式血糖仪比对测试结果的影响研究

目的 探讨抗凝剂物质对便携式血糖仪比对测试结果的影响。方法 根据中华人民共和国国家标准GB/T19634-2005制备低浓度、正常范围、高浓度血糖标本各100份。以肝素锂、乙二胺四乙酸二钾(EDTA-K2)及氟化钠作为抗凝物质,采用拜尔抗RO、强生、罗氏、韩国i-SENS4种便携式血糖仪检测;采用日立全自动生化分析仪检测以上浓度的血糖值。比较不同类型便携式血糖仪比对试验通过率,各便携式血糖仪与生化分析仪血糖检测结果;并分析抗凝剂物质对便携式血糖仪比对测试结果的影响,分析不同类型便携式血糖仪与生化分析仪血糖检测结果的相关性,分析不同类型便携式血糖仪精密度。结果 采用拜尔抗RO、强生稳豪、罗氏、韩国i-SENS便携式血糖仪30台完成血糖仪比对试验,30台便携式血糖仪中3台仪器未通过,通过率为90.0%,其中拜尔抗RO通过率为100.00%,而韩国i-SENS便携式血糖仪通过率最低,为66.67%。拜尔抗RO、强生稳豪、罗氏、韩国i-SENS便携式血糖仪对低浓度、正常范围、高浓度标本的测定结果与生化分析仪比较差异均无统计学意义(P>0.05)。为了分析肝素锂、EDTA-K2及氟化钠不同类型抗凝剂物质对便携式血糖仪测定结果的影响,通过不同血糖浓度下利用4种便携式血糖仪测定,结果表明:血糖浓度1.1~3.3 mmol/L、11.1~16.7 mmol/L、18.1~22.2 mmol/L下拜尔抗RO、强生稳豪、罗氏、韩国i-SENS便携式血糖仪采用肝素锂、EDTA-K2抗凝对血糖测定结果无影响;拜尔抗RO、强生稳豪、罗氏、韩国i-SENS便携式血糖仪采用氟化钠作为抗凝物质测定结果高于肝素锂和EDTA-K2,差异具有统计学意义(P<0.05)。采用Pearson相关性分析软件,以生化分析仪作为因变量,便携式血糖仪为自变量,完成线性回归分析,拜尔抗RO、强生稳豪、罗氏、韩国i-SENS便携式血糖仪的r值分别为0.999、0.994、0.984、0.987。以便携式血糖测定仪重复测定100份标本进行计算,拜尔抗RO、强生稳豪、罗氏、韩国i-SENS便携式血糖仪变异系数(CV)分别为1.49%~2.21%、1.84%~2.51%、5.29%~5.53%和5.13%~6.98%,以CV≤7.5%为标准,所有仪器均合格。结论 拜尔抗RO、罗氏、韩国i-SENS血糖仪的检测一致性较好,而国产强生稳豪的检测结果受到氟化钠抗凝物质的影响,但是均与日立全自动生化分析仪结果存在强相关性,临床检测时应根据需求选择合适的便携式血糖仪,降低内外源性干扰物的影响。

一种便携式荧光测油仪在海上溢油应急监测中的适用性研究

研究了一种便携式荧光测油仪在海上溢油应急监测中的适用性,并与现行仲裁标准方法进行比较分析。研究表明:该便携式荧光测油仪测定海水中油类样品仅需100 mL水样,平均每个样品所需的前处理时间约340 s,方法检出限为0.003 mg/L,测定下限为0.012 mg/L。测定用标准物质配制的不同浓度海水样品,相对标准偏差为0.08%~0.87%,测定标准样品的相对误差为0.49%~4.50%,实际样品加标回收率为90%~107%。对标准物质配制的海水样品进行检测,与现行仲裁标准方法在0.05水平下无显著性差异。在渤海中部海上监测中,连续6 d测定不同标准样品的相对标准偏差为0.26%~0.83%。研究结果表明,该便携式荧光测油仪适用于现场对油类的定量分析和水质评价。

便携式激光诱导击穿光谱仪的研制及其在煤质检测中的应用

目前煤炭企业或火电厂对煤炭质量的检测普遍采用多种离线分析技术,虽然检测精度高,但离线分析程序复杂、分析时间长、信息反馈滞后,不能适应国家低碳经济建设和节能环保的发展战略要求。为满足煤炭现场快速检测的应用目标,开展了基于激光诱导击穿光谱的便携式煤质快速检测装置的研制,从光路的稳定性、设备的紧凑性和光谱定量算法方面开展了设计和优化。所研制的便携式激光煤质快速检测装置具有激光能量高(单脉冲能量>80 mJ),整机重量轻(<10 kg),光谱波段范围宽(200~1100 nm)和重复精度高的特点,可满足煤中所有元素和工业指标的分析。采用该装置对30个电厂常用煤样进行了光谱测试和分析,采用基于马氏距离(MD)的异常数据剔除算法和基于稀疏矩阵的基线估计与降噪算法(BEADS),对煤饼的实测光谱数据进行了预处理,结合支持向量机算法(SVR)对煤炭的碳含量、灰分和热值进行快速检测,SVR模型的拟合系数R 2均能达到0.9999,其中,碳含量的预测均方根误差(RMSEP)为4.5126%,平均相对误差(MRE)为6.74%;灰分的RMSEP为2.8315%,MRE为8.19%;热值的RMSEP为1.4399 MJ/kg,MRE为5.96%。该装置样机可用于多场景下煤质的现场快速检测,对于实现锅炉的燃烧优化、煤炭资源的高效利用和节能减排具有重要意义。

一种便携式呼出气体酒精含量检测仪智能检定装置

本文介绍了一种便携式呼出气体酒精含量检测仪智能检定装置,解决了现有检定装置无法对呼出气体酒精含量检测仪进行现场测量,以及人工读取数据效率低、数据处理过程烦琐的问题。该检定装置实现了呼气中酒精质量浓度与酒精浓度的实时换算,可自动读取和比较酒精质量浓度显示值,为呼出气体酒精含量检测仪测量方法的优化提供了新的思路,也为保障检测仪准确可靠提供了有效的技术支撑。

基于STM32的干式生化电解质分析仪的研究与设计

国产电解质分析仪大多基于湿式法,结构相对复杂,操作过程繁琐,且由于样本外露存在一定的生物安全隐患;针对临床医学上对人体体液中关键电解质成分的检测需要,设计了一种基于干式生化法测试钾离子、钠离子、氯离子的人体电解质分析仪;分析仪基于Cortex-M3嵌入式系统开发,设计了新型干式生化电解质测试片,采用离子选择电极法进行离子测量,结合Nernst方程,提出了一种新的干式电解质测量方法,完成了系统软硬件设计;整机符合医疗仪器行业标准,性能稳定,检测精度高,通过了医疗仪器注册检验;该设计在一定程度上提高了国产电解质分析仪的设计水平,突破了人体电解质干式检测的技术瓶颈,对中小型医院普及人体电解质分析仪具有一定的促进作用。

固定污染源废气中氧化亚氮测定方法研究

为完善温室气体排放监测技术体系,更好服务碳达峰、碳中和目标,建立一种固定污染源废气中氧化亚氮(N2O)的测定方法是非常必要的。利用便携式傅里叶变换红外光谱分析仪测定固定污染源废气中的N2O浓度,对方法的检出限、测定下限、精密度、准确度等技术指标以及仪器抗水分干扰能力进行研究,并开展现场实际样品测试。结果表明,该方法能够准确、连续的检测固定污染源废气中N2O浓度水平,方法检出限为1.0 mg/m^(3)、测定下限为4.0 mg/m^(3),各项技术指标均满足HJ 1011—2018、HJ 168—2020相关要求和实际监测需求。

余氯、总氯现场快速测定仪的研制和应用

研制了一种基于光度法现场测定水中余氯、总氯的手持式仪器 ,开发了相应的专用试剂包 ,讨论了影响该仪器分析性能的主要因素。用该仪器和试剂现场测定水中的余氯和总氯 ,线性范围是 0 .0 1～ 5 .0mg/L ;RSD <3% ;单个样品分析时间不超过 3min。

地震后危重多发伤患者野外条件下的救治体会

目的:探讨突发灾难后危重多发伤患者在野外条件下的救治方案。方法和结果:患者女性,53岁,因"地震中骨盆砸伤、大量血尿"入院,呼吸困难,全腹压痛,褥疮。便携式B超示腹腔积液,左胸腔积液,膀胱破裂。便携式血气分析仪提示"严重贫血+低氧血症+低钾+酸中毒"。术前行左胸腔穿刺,膀胱穿刺引流。剖腹探查见直肠破裂(穿孔),遂行Harttman手术加膀胱造瘘术和抗感染治疗。术后第18日基本恢复。结论:术者具有精湛的技术和丰富的经验、充分发挥便携式设备的作用、依靠具有地理优势的当地医院、积极抗感染是艰苦环境下救治危重多发伤患者的成功因素。

新型高灵敏度XRF分析仪的研制与应用

新型高灵敏度便携式X射线荧光(XRF)分析仪基于电致冷Si-PIN半导体X射线探测器和微机多道(2048道)谱仪系统,整机重3.8kg(探头重0.6kg),对铜、锌、砷等元素的分析检出限为10μg·g-1,适用于野外现场的天然岩石、土壤和水系沉积物的多元素含量的定量测定,以及工业原料、半成品、产品快速分析。

新一代高灵敏度手提式X荧光仪的研制

介绍了新一代高灵敏度手提式X射线荧光仪 ,该仪器由电制冷Si PIN半导体X射线探测器、放射性同位素X射线激发源和基于PC/ 10 4工控机的 4 0 96道多道脉冲幅度分析器组成 ,对铜、锌、砷等元素的分析检出限为 10× 10 -6,适用于野外现场的天然岩石、土壤和水系沉积物的多元素含量的定量测定 ,基本满足新一轮地质大调查的需要 ,特别是二、三级异常点 (带 )的快速查证与野外追索工作。

铁矿石XRF现场取样技术的研究与应用

介绍铁矿石Ｘ射线荧光分析（ＸＲＦ）现场取样的技术及其在武钢金山店铁矿的应用效果。主要讨论ＸＲＦ现场取样中的几何效应、基体效应、水份及矿化不均匀性的影响和校正办法．试验表明，ＸＲＦ现场取样的合格率达到８７．６％，超过了传统的人工取样化验方法的重现性，其速度比传统方法至少提高２００倍，并明显地降低了生产成本，提高了经济效益．

便携式血糖仪与全自动生化分析仪血糖监测的比较研究

目的:探讨便携式血糖仪与大型生化分析仪检测血糖是否存在差异,为临床糖尿病患者及时检测、调控血糖水平等提供快速、简便、准确可靠的血糖检测方法。方法:60例门诊患者同时抽取末梢血与静脉血。末梢血葡萄糖采用酶电极法分别使用当前医院临床使用的2种便携式血糖仪进行测定。静脉血离心后,血清葡萄糖浓度采用葡萄糖氧化酶法使用全自动生化分析仪进行测定。结果:2种便携式血糖仪与大生化分析仪检测血糖水平差异无统计学意义(P>0.05),检测结果之间存在明显相关性,R2分别为0.980 5,0.986 7;2种便携式血糖仪进行比较,检测血糖水平差异无统计学意义(P>0.05),结果之间存在明显相关性,R2=0.960 6。结论:2种便携式血糖仪与全自动生化分析仪检测的血糖结果具有良好的相关性,但应定期对院内便携式血糖仪与实验室全自动生化分析仪的结果进行比对,以确保结果的准确性。