With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oler...With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oleracea were Optimized. The results showed that there were 8 primers suitable for ISSR-PCR of P. oleracea. The optimal reaction system had a volume of 25 μl, including 2 x Taq Platinum PCR Master Mix 12.5 μl, primer 2 μl, ddH20 9.5 μl, and DNA template 1μl. The optimized ISSR-PCR of P. oleracea was started with pre-denaturation at 94 ℃ for 360 s, followed by 30 cycles of denaturation at 94 ℃ for 60 s, annealing at 54 ℃ for 60 s and extension at 72 ℃ for 90 s, and completed by extension at 72 ℃ for 300 s.展开更多
Background:Mosquito is a big threat to the human health.Mosquito-borne diseases cause millions of death to the human beings.Hence,a permanent solution is eagerly to be established to control its excessive growth in st...Background:Mosquito is a big threat to the human health.Mosquito-borne diseases cause millions of death to the human beings.Hence,a permanent solution is eagerly to be established to control its excessive growth in stagnant water.Portulaca oleracea Linn is a natural larvicidal agent,which contains active ingredients such as linolenic acid,linoleic acid(omega-3 fatty acids).These bioactive compounds may be responsible for its larvicidal properties on mosquito.Objective:The present study is focussed on identifying the bioactive compounds,such as linolenic acid,through GC-MS,and analyzing the larvicidal efficacy of Portulaca oleracea L.against Culex quinquefasciatus and Anopheles stephensi larvae.Methods:Preliminary phytochemical analysis,total protein content,total carbohydrate content,total phenol content,total flavonoid content and GC-MS analysis exhibited the presence of rich phytoconstituents in Portulaca oleracea.DPPH analysis was carried out to analyze the antioxidant potential of plant extract.Larvicidal activity and histological change were detected to evaluate the efficacy of Portulaca oleracea L.on the Culex quinquefasciatus and Anopheles stephensi larvae.Results:Aqueous and ethanol leaf extracts of Portulaca oleracea L.against both Culex quinquefasciatus and Anophe-les stephensi larvae,showed a very good larvicidal activity at 500μg/mL among the various concentrations.His-tological damages of mosquito larvae were observed when treated with the Portulaca oleracea L.extract,and provided further evidence for its larvicidal activity.Conclusion:This study concluded that the plant Portulaca oleracea L.contained many useful bioactive compounds,can be a strong larvicidal agent against both Culex quinquefasciatus and Anopheles stephensi larvae.The molecular mechanism for the larvicidal activity will be identified in future studies.展开更多
Objective: Acute liver injury(ALF) is a potential factor of many serious hepatopathies. Carbon tetrachloride(CCl4) is a possible environmental toxicant that can induce ALF. Portulaca oleracea(PO) is one of the most po...Objective: Acute liver injury(ALF) is a potential factor of many serious hepatopathies. Carbon tetrachloride(CCl4) is a possible environmental toxicant that can induce ALF. Portulaca oleracea(PO) is one of the most popular edible herbs and has several biological activities such as antioxidant, antimicrobial, antiinflammatory effects. We explored the significance of PO in regulating inflammatory function in animal models and cultured hepatocytes during liver damage caused by CCl4.Methods: The effect of PO on ALF was evaluated by CCl4-induced mice models in vivo. Hepatic levels of transaminase activities and inflammatory factors were examined. The gene and protein expression of S100A8 and S100A9 were measured by RT-PCR and Western blot analysis. Meanwhile, the efficacy of PO was certified by HepG2 cells in vitro. The transaminase activities, inflammatory factors, and the protein expression of S100A8 and S100A9 were also detected.Results: Animal tests showed that pretreatment with PO reduced the liver pathological tissue damage and the serum levels of ALT, AST, ALT and LDH, as well as reducing the pro-inflammatory cytokines(IL-1β, IL-6, TNF-a) secretion in CCl4-induced liver injury mice. Simultaneously, Hep G2 cells pretreated with PO exhibited a significant decrease in the activities of ALT and AST. Moreover, PO resulted in a significant downregulation of the pro-inflammatory markers S100A8, S100A9 gene and protein expression on CCl4induced acute liver injury was demonstrated entirely in vivo and vitro experiments.Conclusion: PO may down-regulate S100A8 and S100A9 and inhibit pro-inflammatory cytokines’ release,indicating a potential clinical effect for controlling the disease.展开更多
Purslane, a common weed, has been used as food or folk medicine in many countries. The growth, medicinal components and nutrient contents of the plant are closely associated with endophytes, especially endophytic fung...Purslane, a common weed, has been used as food or folk medicine in many countries. The growth, medicinal components and nutrient contents of the plant are closely associated with endophytes, especially endophytic fungi. In this study, the endophytic fungi associated with purslane were isolated, and the effects of the isolates on the host were investigated to lay a foundation for further research and development of purslane resources. The results showed that a total of eight endophytic fungi were isolated from purslane (collected from Hohhot, Inner Mongolia, China), and they belonged to the genera Penicillium (isolates K, N, P, M and I), Chaetomium (isolate J), Fusarium (isolate H) and Petriella (isolate O). Moreover, the growth of purslane was significantly influenced by its endophytic fungi. Isolate M can significantly decrease the germination rate, while J can significantly increase the germination rate of purslane. In addition, H, J and M can significantly increase the bud length of purslane, and the fermentation broth of P has a negative influence on the bud length of purslane. M and I can significantly increase the height, fresh weight and chlorophyll content of purslane due, in part, to the lower pH of the fermentation broth of I and M.展开更多
文摘With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oleracea were Optimized. The results showed that there were 8 primers suitable for ISSR-PCR of P. oleracea. The optimal reaction system had a volume of 25 μl, including 2 x Taq Platinum PCR Master Mix 12.5 μl, primer 2 μl, ddH20 9.5 μl, and DNA template 1μl. The optimized ISSR-PCR of P. oleracea was started with pre-denaturation at 94 ℃ for 360 s, followed by 30 cycles of denaturation at 94 ℃ for 60 s, annealing at 54 ℃ for 60 s and extension at 72 ℃ for 90 s, and completed by extension at 72 ℃ for 300 s.
文摘Background:Mosquito is a big threat to the human health.Mosquito-borne diseases cause millions of death to the human beings.Hence,a permanent solution is eagerly to be established to control its excessive growth in stagnant water.Portulaca oleracea Linn is a natural larvicidal agent,which contains active ingredients such as linolenic acid,linoleic acid(omega-3 fatty acids).These bioactive compounds may be responsible for its larvicidal properties on mosquito.Objective:The present study is focussed on identifying the bioactive compounds,such as linolenic acid,through GC-MS,and analyzing the larvicidal efficacy of Portulaca oleracea L.against Culex quinquefasciatus and Anopheles stephensi larvae.Methods:Preliminary phytochemical analysis,total protein content,total carbohydrate content,total phenol content,total flavonoid content and GC-MS analysis exhibited the presence of rich phytoconstituents in Portulaca oleracea.DPPH analysis was carried out to analyze the antioxidant potential of plant extract.Larvicidal activity and histological change were detected to evaluate the efficacy of Portulaca oleracea L.on the Culex quinquefasciatus and Anopheles stephensi larvae.Results:Aqueous and ethanol leaf extracts of Portulaca oleracea L.against both Culex quinquefasciatus and Anophe-les stephensi larvae,showed a very good larvicidal activity at 500μg/mL among the various concentrations.His-tological damages of mosquito larvae were observed when treated with the Portulaca oleracea L.extract,and provided further evidence for its larvicidal activity.Conclusion:This study concluded that the plant Portulaca oleracea L.contained many useful bioactive compounds,can be a strong larvicidal agent against both Culex quinquefasciatus and Anopheles stephensi larvae.The molecular mechanism for the larvicidal activity will be identified in future studies.
基金supported by Independent Research Projects for young teachers of Minzu University of China [No. 2021NQPY90]。
文摘Objective: Acute liver injury(ALF) is a potential factor of many serious hepatopathies. Carbon tetrachloride(CCl4) is a possible environmental toxicant that can induce ALF. Portulaca oleracea(PO) is one of the most popular edible herbs and has several biological activities such as antioxidant, antimicrobial, antiinflammatory effects. We explored the significance of PO in regulating inflammatory function in animal models and cultured hepatocytes during liver damage caused by CCl4.Methods: The effect of PO on ALF was evaluated by CCl4-induced mice models in vivo. Hepatic levels of transaminase activities and inflammatory factors were examined. The gene and protein expression of S100A8 and S100A9 were measured by RT-PCR and Western blot analysis. Meanwhile, the efficacy of PO was certified by HepG2 cells in vitro. The transaminase activities, inflammatory factors, and the protein expression of S100A8 and S100A9 were also detected.Results: Animal tests showed that pretreatment with PO reduced the liver pathological tissue damage and the serum levels of ALT, AST, ALT and LDH, as well as reducing the pro-inflammatory cytokines(IL-1β, IL-6, TNF-a) secretion in CCl4-induced liver injury mice. Simultaneously, Hep G2 cells pretreated with PO exhibited a significant decrease in the activities of ALT and AST. Moreover, PO resulted in a significant downregulation of the pro-inflammatory markers S100A8, S100A9 gene and protein expression on CCl4induced acute liver injury was demonstrated entirely in vivo and vitro experiments.Conclusion: PO may down-regulate S100A8 and S100A9 and inhibit pro-inflammatory cytokines’ release,indicating a potential clinical effect for controlling the disease.
文摘Purslane, a common weed, has been used as food or folk medicine in many countries. The growth, medicinal components and nutrient contents of the plant are closely associated with endophytes, especially endophytic fungi. In this study, the endophytic fungi associated with purslane were isolated, and the effects of the isolates on the host were investigated to lay a foundation for further research and development of purslane resources. The results showed that a total of eight endophytic fungi were isolated from purslane (collected from Hohhot, Inner Mongolia, China), and they belonged to the genera Penicillium (isolates K, N, P, M and I), Chaetomium (isolate J), Fusarium (isolate H) and Petriella (isolate O). Moreover, the growth of purslane was significantly influenced by its endophytic fungi. Isolate M can significantly decrease the germination rate, while J can significantly increase the germination rate of purslane. In addition, H, J and M can significantly increase the bud length of purslane, and the fermentation broth of P has a negative influence on the bud length of purslane. M and I can significantly increase the height, fresh weight and chlorophyll content of purslane due, in part, to the lower pH of the fermentation broth of I and M.
